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Books > Science & Mathematics > Biology, life sciences > Cellular biology
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HPLC in Enzymatic Analysis 2e (Hardcover, 2nd Edition)
Loot Price: R9,388
Discovery Miles 93 880
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HPLC in Enzymatic Analysis 2e (Hardcover, 2nd Edition)
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The use of High Performance Liquid Chromatography (HPLC) techniques
in the study of enzymatic reactions has grown significantly since
the publication of the first edition of this highly successful
book: the role of enzymes in biological research has expanded; the
application of HPLC and enzymes has extended to more disciplines;
advances in separation techniques and instrumentation have
increased the capability of HPLC; and the discovery of new enzymes
has spawned new methods of analysis.
High Performance Liquid Chromatography in Enzymatic Analysis,
Second Edition addresses these developments in its coverage of the
refinements of HPLC methods and their use in a wide range of
laboratory applications. It offers the same practical approach
found in the first edition, incorporates a wealth of new
information into existing chapters, and adds new chapters to deal
with new applications, including capillary electrophoresis,
forensic chemistry, microdialysis, and the polymerase chain
reaction. Topics include:
* Application of HPLC to the assay of enzymatic activities
* Concepts and principles of HPLC, including the latest
technological advances
* Concepts and principles of capillary electrophoresis (CE)
* Strategy for design of an HPLC/CE system for assay of enzyme
activity
* Preparation of enzymatic activities from tissues and single
cells
* Analysis of enzymatic activities in body fluids, including
chromatobiosis
* HPLC for the identification of new enzymatic activities
* Fundamentals of the polymerase chain reaction
* HPLC in forensics
* Survey of enzymatic activities assayed by the HPLC method,
including many new categories
* Multienzymesystems, including many new examples
* HPLC in the analysis of contaminated food
"It is the ability of HPLC to accomplish separations completely
and rapidly that led to its original application to problems in the
life sciences, particularly those related to purification. An
analysis of the literature revealed that this technique was used
primarily for the purification of small molecules, macromolecules
such as peptides and proteins, and more recently, antibodies. This
application to purification has all but dominated the use of the
method, and there has been a plethora of books, symposia, and
conferences on the use of HPLC for these purposes. However, it was
only a matter of time before others began to look beyond and to
explore the possibilities that result from the capacity to make
separations quickly and efficiently." --from the preface to the
First Edition
Easy to read and full of practical advice and hundreds of diagrams
and examples, High Performance Liquid Chromatography in Enzymatic
Analysis, Second Edition is an invaluable resource for students,
researchers, and laboratory workers in analytical chemistry and
biochemistry, molecular biology and cell biology, and for anyone
interested in keeping up with this fast-growing field.
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