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Modern Vibrational Spectroscopy & Micro-Spectroscopy - Theory, Instrumentation & Biomedical Applications (Hardcover)
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Modern Vibrational Spectroscopy & Micro-Spectroscopy - Theory, Instrumentation & Biomedical Applications (Hardcover)
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Modern Vibrational Spectroscopy and Micro-Spectroscopy: Theory,
Instrumentation and Biomedical Applications unites the theory and
background of conventional vibrational spectroscopy with the
principles of microspectroscopy. It starts with basic theory as it
applies to small molecules and then expands it to include the large
biomolecules which are the main topic of the book with an emphasis
on practical experiments, results analysis and medical and
diagnostic applications. This book is unique in that it addresses
both the parent spectroscopy and the microspectroscopic aspects in
one volume. Part I covers the basic theory, principles and
instrumentation of classical vibrational, infrared and Raman
spectroscopy. It is aimed at researchers with a background in
chemistry and physics, and is presented at the level suitable for
first year graduate students. The latter half of Part I is devoted
to more novel subjects in vibrational spectroscopy, such as
resonance and non-linear Raman effects, vibrational optical
activity, time resolved spectroscopy and computational methods.
Thus, Part 1 represents a short course into modern vibrational
spectroscopy. Part II is devoted in its entirety to applications of
vibrational spectroscopic techniques to biophysical and
bio-structural research, and the more recent extension of
vibrational spectroscopy to microscopic data acquisition.
Vibrational microscopy (or microspectroscopy) has opened entirely
new avenues toward applications in the biomedical sciences, and has
created new research fields collectively referred to as Spectral
Cytopathology (SCP) and Spectral Histopathology (SHP). In order to
fully exploit the information contained in the micro-spectral
datasets, methods of multivariate analysis need to be employed.
These methods, along with representative results of both SCP and
SHP are presented and discussed in detail in Part II.
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