Advances in Microbial Physiology, Volume 78, the latest release in this ongoing series, continues the long tradition of topical, important, cutting-edge reviews in microbiology. This updated release contains the latest information in the field, with comprehensive chapters covering Microbubble Intensification of Bioprocessing, Bacterial cellulose: biosynthesis, production, and applications, Microbial energy management - a product of three broad tradeoffs, and more.

Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Apoptosis in Health and Disease - Part B, Volume 126 in the Advances in Protein Chemistry and Structural Biology focuses on apoptotic responses in numerous conditions - from bacterial and parasite infections, to pathological states such as oxidative stress, pulmonary hypertension, and different cancer types, etc. In addition, the book provides therapeutic strategies for targeting apoptosis. These new advanced understandings are playing a major influence in drug discovery and the introduction of new therapies that target the cell death process. Apoptosis, or programmed cell death, is the mechanism by which cells die either physiologically or pathologically. Vast research in apoptosis has advanced our understanding of basic physiological and pathological processes occurring in cells, organs and organisms, and its role in a number of diseases.

Advances in Clinical Chemistry, Volume 102, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians and research scientists. The serial discusses the most up-to-date technologies related to the field of clinical chemistry. Chapters in this new release cover Advances in immunosensor technology, Extracellular Vesicles: Roles and Applications in Drug-Induced Liver Injury, Oxidative stress biomarkers in the preterm infant, Translational biomarkers in the era of precision medicine, Metabolomics applications in coronary artery disease personalized medicine, Quantitative EEG biomarkers for epilepsy and their relation to chemical biomarkers.

Herbal Biomolecules in Healthcare Applications presents extensive detailed information on all the vital principles, basics and fundamental aspects of multiple herbal biomolecules in the healthcare industry. This book examines important herbal biomolecules including alkaloids, glycosides, flavonoids, anthraquinones, steroids, polysaccharides, tannins and polyphenolic compounds, terpenes, fats and waxes, proteins and peptides, and vitamins. These herbal biomacromolecules are responsible for different bioactivities as well as pharmacological potentials. A systematic understanding of the extraction, purification, characterization, applications of these herbal biomolecules and their derivatives in healthcare fields is developed in this comprehensive book. Chapters explore the key topics along with an emphasis on recent research and developments in healthcare fields by leading experts. They include updated literature review of the relevant key topics, good quality illustrations, chemical structures, flow charts, well-organized tables and case studies. Herbal Biomolecules in Healthcare Applications will be useful for researchers working on natural products and biomolecules with bioactivity and nutraceutical properties. Professionals specializing in scientific areas such as biochemistry, pharmacology, analytical chemistry, organic chemistry, clinics, or engineering focused on bioactive natural products will find this book useful.

Biological Experiments in Space: 30 Years Investigating Life in Space Orbit covers investigations of plant, algae, animals, fish, microorganisms and tissue cultures on space flights, beginning with the first orbital space station on Salyut 1. The book includes results on the influence of the entire complex of physical factors associated with spaceflight on biological systems, including analysis of the impact of microgravity on organisms, as well as the effects of electric and magnetic fields. This book offers important insights for researchers of space biology and astrobiology, as well as space agency and industry specialists developing future space stations and missions. Lack of gravity, temperature and chemical gradients, magnetic and electrical fields, spectral composition and intensity of light, and high-energy cosmic radiation influence many important metabolic and physiological processes in animals, plants, and microorganisms, as well as transfer phenomena in and around them. Success of future space exploration depends on understanding the effects of these factors on biological organisms and developing appropriate countermeasures, aimed at improving growth, development, and reproduction in microgravity.

mRNA 3' End Processing and Stability, Volume 655 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics. Each chapter is written by an international board of authors.

Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems Part B, represents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. It is a simple, rapid, precise, and cost-effective tool for genome editing and regulation of a wide range of organisms. It has gained scientific and public attention worldwide. This volume mainly covers insect cell line, protozoans, zebrafish, drosophila, CRISPRi, patents as well as technology transfer, and many more. This book is a key source of information available in a single volume. This book will be useful for not only beginners in genome engineering, but also students, researchers, scientists, policymakers, and stakeholders interested in harnessing the potential of reprogramming of the genomes in several areas.

Ion Channels, Part C, Volume 653 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Nonsense suppression in ion channels, Engineering Ion Channels Using Protein Trans-splicing, Probing Ion Channel Neighborhoods Using APEX, STX based probes for NaVs, ANAP: a versatile, fluorescent probe of ion channel gating and regulation, High Throughput Screens for Small Molecule Ion Channel Modulators, Using toxins to study ion channels, Re/de-constructing ubiquitin regulation of ion channels, Tethered Peptide Toxins for Ion Channels, Voltage-Sensing Phosphatase Molecular Engineering, and more. Additional chapters cover Engineering excitable cells, Stretch and Poke Stimulation of Mechanically-Activated Ion Channels, Optical Control of STIM Channels, High Throughput Electrophysiological Evaluation of Mutant Ion Channels, Evaluating BEST1 Mutations in RPE Stem Cells, Long Read Transcript Profiling of Ion Channel Splice Variants, Permeation of Connexin Channels, Ratiometric pH indicator for melanosomes and lysosomes, and Ion channels in the epithelial cells of the choroid plexus.

Apoptosis, or programmed cell death, is the mechanism by which cells die either physiologically or pathologically. A vast research in apoptosis has advanced our understanding of basic physiological and pathological processes occurring in cells, organs and organisms, and its role in a number of diseases. These new advanced understandings are playing a major influence in drug discovery and the introduction of new therapies that target this cell death process. These two thematic volumes 125 and 126 of the Advances in Protein Chemistry and Structural Biology focus on apoptotic responses in numerous conditions - from bacterial and parasite infections to pathological states such as oxidative stress, pulmonary hypertension, different cancer types, etc. Finally, therapeutic strategies for targeting apoptosis are also discussed.

Ion Channels Part B, Volume 652 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including NMDAR, Pannexin, and CALHM, Making NaV1.4 and NaV1.7, TRPVs, Purification native nAChRs, GABAR Radu Aricescu, TRPV5/2, NaV1.5, KATP, TRPA1, TREK-1, SARS-CoV-2 3a ion channel, Ion channel conformational dynamics by encoded unnatural amino acid, Fluorescence lifetime measurement of absolute membrane potential, Fluorescent Toxins as Activity Sensors, FRET Analyses of Ion Channel Protein-Protein Interactions, Control of Ion Channel Gating with Photo-Switchable Tweezers, and Counting Subunits in Kv Channel Complexes.

Curing Genetic Diseases through Genome Reprogramming, Volume 182 captures an historic moment in the field of gene therapy-the dawn of a new age in which the dream of curing genetic diseases has become realizable. The volume presents the most clinically advanced gene therapy and genome editing approaches for the treatment of genetic diseases in specific organs, including difficult therapeutic targets, futuristic ideas of genetic interventions, and large scale human genome repair. An initial chapter addresses the complex ethical aspects involved in the very idea of modifying the human genome.

Ion Channels Part A, Volume 651 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of new developments on the topic. Each chapter is written by an international board of authors.

Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems, Part A presents a collation of chapters written by global, eminent scientists. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. Chapters in this release include An Introduction and applications of CRISPR-Cas Systems, History, evolution and classification of CRISPR-Cas associated systems, CRISPR based bacterial genome editing and removal of pathogens, CRISPR based genome editing and removal of human viruses, CRISPR based development of RNA editing and diagnostic platform, and much more. Additional sections cover Genome engineering in insects for control of vector borne diseases, Development of insect cell line using CRISPR technology, CRISPRing protozoan parasites to better understand the biology of diseases, CRISPR based genome editing of Caenorhabditis elegans, and a variety of other important topics.

Vitamins and Hormones serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Pore Forming Toxins, Volume 649 in the Methods in Enzymology series continues the legacy of this premier serial with quality chapters authored by field leaders. Chapters in this new release include X-ray crystallography shines a light on pore-forming toxins, Giant unilamellar vesicles for studying activity of pore forming proteins, Combined applications of fluorescence spectroscopy and molecular dynamics simulations in studies of Diphtheria Toxin translocation domain, Biophysical approaches to study actinoporin-lipid interactions, Molecular basis for activation of Actinoporins by lipids, Engineered ClyA for detection of biological molecules, Pore-forming Toxins for the Size-Discrimination of Polymers and Biopolymers: Towards Biomolecules Sequencing, and much more.

Advances in CRISPR/Cas and related technologies, Volume 179, the latest release in this ongoing series, deals with a wide variety of research topics related to recent advancement in the genome editing techniques. Associated chapters in this new release include Challenges for Therapeutic application of CRISPR Cas techniques, Mitochondrial DNA modification by CRISPR Cas System: Challenges and future direction, Trends in CRISPR Cas technology application in cancer, Modified CRISPR-Cas for next generation application, Application of CRISPR Cas in Synthetic Biology: Challenges and Scopes, History of CRISPR Cas system from bacterial Adaptive Immune System to research application, and more.

Hormones and Aging, Volume 115 in the Vitamins and Hormones series, highlights advances in the field, with this new volume presenting timely topics, including hypothalamic aging and hormones, endocannabinoids and aging-inflammation, neuroplasticity, mood and pain, the impact of hormones and bone loss across the menopause transition, and much more.

Advances in Clinical Chemistry, Volume 101, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians and research scientists. The serial discusses the latest and most up-to-date technologies related to the field of clinical chemistry, with this new release including chapters on Exosomes in cancer, Parathyroid hormone, Clinical relevance of biochemical and metabolic changes in osteoarthritis, Early diagnosis with ultrasensitive ELISA, Cytokines and the immune response in obesity-related disorders, Metabolic profiling of organic and fatty acids in chronic and autoimmune diseases, and more.

Metal and Nutrient Transporters in Abiotic Stress focuses on the different forms of environmental stress related to heavy metal, metalloid and nutrient deficiency that have the potential to inflict major damages to crop plants, leading to a massive decrease in crop yield and productivity. The book presents the current state of knowledge of the biochemical and molecular regulation of several classes of membrane transporters related to the uptake of metals/metalloids and nutrient elements during different stresses and their probable mechanisms of operation in plant stress tolerance. Metal and Nutrient Transporters in Abiotic Stress provides a comprehensive discussion that will help in mitigating multiple forms of stresses utilizing transporter proteins. Edited by leading experts and written by a global team of knowledgeable contributors, this book will further stimulate research in the field of transporter proteins and will foster further interests for researchers, academicians and scientists worldwide. It is complimented by its companion book titled Transporters and Plant Osmotic Stress.

Protein Kinases in Drug Discovery, Volume 124 discusses the latest information on protein kinases and how they modify other proteins by chemically adding phosphate groups to them. New chapters in this release include Transport Proteins and AMPs: Implications in Human Disease, Protein kinase CK2 inhibition as a pharmacological strategy, Emerging role of Protein kinase in diabetes mellitus: From Mechanism to therapy, Dual Roles of ATP-binding site in Protein Kinases: Orthosteric inhibition and Allosteric Regulation, Pseudokinases in drug discovery and development: progress, challenges and future prospects, Comparison of knowledge-based vs. combinatorial peptide library approaches for the identification of protein kinase substrates, and more.

Methods in Enzymology, Volume 650 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Biophysical methods to study lanthanide-protein interactions, Genetically encoded sensors to study lanthanide biology, Spectrophotometric methods to determine the stability constants of lanthanide-macromolecule complexes, Lanthanide-based probes for amino acid modifications, In vitro selection and application of lanthanide-dependent DNAzymes, LRET biosensors for imaging protein interactions in living cells, Synthetic Modeling of the Structure and Function of the Lanthanide-Dependent, MDH Cofactor, EPR spectroscopy of lanthanides, Macromolecular crystallography for f-element complex characterization, and much more.

Advances in Clinical Chemistry, Volume 100, the latest installment in this internationally acclaimed series, contains chapters authored by world-renowned clinical laboratory scientists, physicians and research scientists. The serial discusses the latest and most up-to-date technologies related to the field of clinical chemistry, with chapters in this new release focusing on Chimeric RNAs in cancer, Pathophysiologic landscape of osteoarthritis, Advances in lung cancer biomarkers: The role of (metal-) metabolites and selenoproteins, Advances in cerebral palsy biomarkers, Cytokines in psoriasis, Biochemical analysis of oral fluids for disease detection, and more.