Proceedings of an international workshop held in Hamburg, Germany, May 19-23, 1996

Since the advent of the Human Genome Project, an increasing number of disease-causing genes have been discovered and, in some cases, genetic tests developed. However, this is only the first step. The second, much larger phase is the analysis of the total sequence. What does the rest of the DNA do? The answer to this question will be determined by computer prediction, expression profiling, and comparative genome analysis. Comparative Genomics covers such topics as identifying novel genes, determining gene function, control sequences, and developmental switches. The book aims to demonstrate how different approaches taken with model organisms, such as mutation studies, expression profiling of cDNAs, in situ localization of message and comparative genome analysis (both at the gene and nucleotide level) will aid in our understanding of the results coming out of the Human Genome Project and contribute significantly to our understanding of how genes function.

People have relied on medicinal products derived from natural sources for millennia, and animals have long been an important part of that repertoire; nearly all cultures, from ancient times to the present, have used animals as a source of medicine. Ingredients derived from wild animals are not only widely used in traditional remedies, but are also increasingly valued as raw materials in the preparation of modern medicines. Regrettably, the unsustainable use of plants and animals in traditional medicine is recognized as a threat to wildlife conservation, as a result of which discussions concerning the links between traditional medicine and biodiversity are becoming increasingly imperative, particularly in view of the fact that folk medicine is the primary source of health care for 80% of the world's population. This book discusses the role of animals in traditional folk medicine and its meaning for wildlife conservation. We hope to further stimulate further discussions about the use of biodiversity and its implications for wildlife conservation strategies.

Based on the author's more than 40 years experience, Bacterial Growth and Form examines such important questions as what bacteria were, what they are, and what they do. Particular emphasis is placed on the ability of bacteria to establish their shapes as they grow and divide. By developing an understanding of the properties of these simple and early life forms, especially at the levels of physics and mathematics, the book provides insight into the mechanism used by bacteria to subvert physical forces to their own ends. A major consideration of this work is that prokaryotes do many of the same things that eukaryotes do, but with simpler equipment employed in an extremely sophisticated way. The book illustrates this point by closely examining the basic mechanismof hydrostatic or turgor pressure: how it functions for many of the mechanical purposes in the prokaryote, how it leads to mechanisms for resisting turgor pressure, and how it ultimately led to the development of exoskeletons and endoskeletons, and to the refinement of bacteria. Bacterial Growth and Form brings together biochemical, biophysical, and physiological principles in an authoritative, single-source volume. It provides researchers, and students in biophysics and microbiology with an indispensible reference and a new perspective into the biology of life.

This book is about video techniques, not video technology. To deal with the latter would be a thankless task, as by the time the book was published it would be out of date, given the rapid rate of development of video hardware. However, these technological advances do help to make it an exciting field. As Joe Riley says in Chapter 1, 'Advances in video technology continually produce improvements in performance and reductions in both the cost and size of equipment, so it seems certain that the technique will prove to be an even more useful resource . . . in the future. ' In selecting the topics and authors represented in this book, I have tried to encompass most of the behavioural and ecological uses to which video is likely to be put over the next decade or so. I believe the book has captured the invaluable accumulated experience of the most active practitioners of the medium in this research area. The idea for the book arose from two workshops on the use of video in ecology and behaviour held at Southampton University, UK, during the 1980s. Three learned societies were involved in these meetings: the Association of Applied Biologists, the British Ecological Society and the Society for Experimental Biology.

As an industry, biotechnology may be likened to the Hymn Book, being both ancient and modern. Whereas activities such as baking, brewing, the fermenting of foods date from our earliest attempts to control and utilise the environment, the application of recombinant DNA technology is recognised as being at the forefront of novel industrial development. Perhaps because of its association with processing foodstuffs together with the benefits derived from applications in the early organic chemistry and pharmaceutical industries, biotechnology has been regarded as being inherently safe. Yet unlike other modern industries, such as chemical and nuclear, where regulation has followed from incidents or accidents, modern biotechnology has been subject to close scrutiny and regulation almost from its inception. The process of regulation itself is somewhat unusual in that it was initially self-imposed by the very scientists who developed the fundamental techniques of recombinant DNA technology. They recognised the signific ance of their development but were concerned of the effects on humans and the environment of uncontrolled application of the new, powerful technology. Concern about the possible consequences of genetic manipula tion has undoubtedly been the driving force behind the regulations that are now in place in many parts of the world and which are the subject of this book. Safety issues in the biotechnology industry can be categorised under three headings: worker, environmental and consumer (product) safety."

The Symposium on Swimming and Flying in Nature which was held at the California Institute of Technology, Pasadena, California from July 8-12, 1974 was conceived with the objective of providing an interdisciplinary forum for the discussion of funda mental biological and fluid mechanical aspects of these forms of natura110comotion. It was the earnest hope of all concerned in the organization of the Symposium that the exchange of knowledge and interaction of ideas from the disciplines involved would stimu late new research in this developing field. If the liveliness of the discussion generated among the 250 or so participants is any measure, then this objective was fulfilled to a significant degree. These two companion volumes contain the manuscripts of the papers presented during the Symposium. It is hoped that this permanent record will serve to perpetuate the enthusiasm and active thought generated during those days in Pasadena. The first volume contains the proceedings of the first two days of the confer ence (Sessions I to IV) which concentrated on the locomotion of micro-organisms. The second volume (Sessions V to VIII) deals with the propulsion of larger fish, insects and birds. Professor Sir James Lighthill's Special Invited Lecture which opened the Symposium is contained in the second volume, rather than the first, since it deals with natural flight."

This collection of 29 papers grew out of a symposium entitled "Setting the Future Agenda for Neotropical Primates. " The symposium was held at the Department of Zoo logical Research, National Zoological Park, Washington D. C., on February 26-27, 1994, and was sponsored by the Wenner-Gren Foundation for Anthropological Research, Smith sonian Institution, and Friends of the National Zoo. We put the symposium together with two objectives: to honor Warren G. Kinzey for his contributions to the growing field of platyrrhine studies and to provide researchers who work in the Neotropics with the oppor tunity to discuss recent developments, to identify areas of research that require additional study, and especially to help guide the next generation of researchers. The symposium provided the opportunity to recognize Warren as a mentor and col laborator to the contribution of the study of platyrrhines. Contributions to the book were expanded in order to provide a more comprehensive view of platyrrhine evolution and ecology, to emphasize the interdisciplinary nature of many of these studies, and to high light the central role that New World monkeys play in advancing primatology. If this vol ume were to require major revisions after just one more decade of research, that would be a fitting testament to Warren's enthusiasm and his drive to continually update the field with new ideas and methods. Tributes to Warren and a list of his publications have been published elsewhere (Norconk, 1994, 1996; Rosenberger 1994, 1995)."

Although interest in evolutionary novelties can be that these different mechanisms cooperate in the mak traced back to the time of Darwin, the appreciation ing of new genes. In the second phase of new gene evolution, conventional models of new gene evolution, and systematical experimental pursuit of the origin and evolution of new gene functions did not appear for example by gene duplication, held that the muta until the early years of last decade. Since the 1970s, tions fixed in the early stages of the new genes are Susumu Ohno, Walter Gilbert, and others from the assumed to be neutral or nearly neutral. However, it area of evolutionary genetics have made pioneer ef appears that the force of Darwinian positive selection has been detectably strong from the outset in avail forts to elaborate possibilities for major biological mechanisms, for example, gene duplication and exon able population genetic studies of young genes created through the process of exon recombination. This may shuffling, by which new gene functions could arise. However, the problem of new gene evolution did not account for a common phenomenon in phylogenetic catch significant attention among biologists generally analyses of genes with changed functions: the early even recently. One of the reasons was the lack of ex stages of such genes are usually associated with accel perimental or observational systems for investigating erated substitution rates. Nonetheless, a more general factual details of the 'birth' process of new genes.

Planthoppers include some of the most devastating pests of major agricultural crops throughout the world. One species, the rice brown planthopper, is among the most economically important pests in Asia. In past decades, government policies encouraged the control of rice planthoppers with synthetic pesticides, a tactic which promoted insecticide resistance and often led to the pesticide-induced resurgence of pest populations. To deter planthopper outbreaks, a more ecologically sound management strategy is being implemented, one based on a thorough investigation of population dynamics, natural enemies, and the genetics of host plant and insecticide adaptation. In the natural habitats of North America and Europe, scientists have also used planthoppers as model organisms to test ecological and evolutionary theory. The consequence of these diverse studies is an extremely scattered literature on planthoppers that has never been synthesized from an ecological perspective. This volume summarizes what is known about planthopper ecology and biological control. It takes a theoretical approach yet is deeply concerned with the application of theory to the practical problems of pest management.

Morphometrics is concerned with the study of variations and change in the form (size and shape) of organisms or objects adding a quantitative element to descriptions and thereby facilitating the comparison of different objects and organisms. This volume provides an introduction to morphometrics in a clear and simple way without recourse to complex mathematics and statistics. This introduction is followed by a series of case studies describing the variety of applications of morphometrics from paleontology and evolutionary ecology to archaeological artifacts analysis. This is followed by a presentation of future applications of morphometrics and state of the art software for analyzing and comparing shape.

The International Symposium on Heparin, held May 13-15, 1974, in St. Louis, Missouri, as a part of the dedication of the Shoenberg Pavilion of the Jewish Hospital of St. Louis, was conceived as a forum to bring together physicians and scientists with a basic in terest in the structure, function and clinical usefulness of heparin. Few naturally occurring substances have commanded the breadth of interest among members of the biomedical research community as this compound has. Aspects of its covalent and three-dimensional struc ture, its biosynthesis, its interaction with and effect on physio logically important moieties and its use as a therapeutic agent in a variety of disease states have been actively studied for the past several decades. Thus, the present state of these studies seemed to be a timely subject for discussion, not only to gather together in one place representative samples of the myriad of data on heparin but also to underscore the ever increasing necessity for communica tion between basic research and clinical practice."

Phytophagous insects represent a very particular not really belong to their host plant range. This may group of organisms. Firstly, their number amounts lead to mistaken conclusions especially in regions to more than one quarter of all recent species (ex where only few observations were possible, as well cluding fungi, algae and microbes) and together with as in the case of uncommon insect species. Fourthly, the green plants on which they feed they form al the great majority (99. 4%) of the agromyzid species most one half of all living species described so far. studied show a high degree of host specialization Secondly, their overwhelming majority shows very which makes these insects especially suitable for narrow host plant specialization, that is they feed taxonomic-phylogenetic considerations. only on one or a few, mostly closely related plant With such an enormous amount of data, it may species, a characteristic that led J. H. Fabre to elab have been tempting to draw far-reaching conclu orate the notion of the 'insects' botanical instinct' a sions. However, the author has been very careful in century ago. doing this."

The development of specialised feeding habits during the course of time by human beings is paralleled in the majority of animals, in particular have developed special peculiarities, and insect larvae which in most cases are quite characteristic of the species concerned. This applies especially to phytophagous insect larvae, and anyone with the requisite experience can say with a fair degree of certainty which insect larva is responsible for any damage to be found on a plant. It leaves behind a definite "feeding pattern" which might be compared to a "visiting card" on which the genus and species are marked in runic characters. Whoever has learned to read the runes can readily determine who has been feeding on the affected spot, solely on the basic of the "visiting card" left behind. From the known factors - the name of the plant and the type of feeding patter- and after some study of the various types of plant infestation, both the genus and species of the larva producing the feeding pattern can be worked out without difficulty. The importance of "feeding pattern investigation" has now far outstripped the successes to be obtained by normal collecting. Previously, when wishing to list the species of insects present in any given locality they were caught with the net, by sugaring and other methods. This always resulted in a very defective "list" of the insects in fact existing in the locality concerned.

Increasing rate of species extinction in the present day will lead to a huge biodiversity crisis; eventually, this will lead to the paucity of non-renewable resources of energy making our Earth unsustainable in future. To save our mother planet from this crisis, studies need to be performed to discover abundant new fossil sites on Earth for continued access to oil-rich locations. Most importantly, a holistic approach is necessary in solving the present problem of biodiversity loss. This book presents newly developed quantitative models in understanding the biodiversity, evolution and ecology of extinct organisms. This will assist future earth scientists in understanding the natural and anthropogenic causes behind biodiversity crisis and ecosystem collapse. In addition, this study would be of great interest to exploration geologists and geophysicists in potentially unraveling natural resources from our sustainable Earth.

This is the third book chronicling the scientific activities of the European Society for Photobiology (ESP). It contains 56 chapters, written by authors from 16 countries, based on presentations at the 3rd Congress of the European Society for Photobiology held in Budapest, Hungary on the 27th August - 2nd September 1989. The science of photobiology, which can simply be defined as the study of the effects of light on living matter, covers so many subject areas that no single book can hope to do justice to them all. This multidisciplinary nature of photobiology is reflected by the material covered in this volume, which contains chapters on such diverse themes as motile photoresponses in bacteria, cancer therapy and photosynthesis. Interestingly, the emphasis placed on various subject areas differs quite markedly from the preceding volume ('Light in Biology & Medicine, volume 1, eds. R. Douglas, J. Moan & F. Dall'Acqua, Plenum Press, 1988). It is hoped that by highlighting different areas of photobiology these and future pUblications emanating from the ESP will, in time, produce a comprehensive record of photobiological research, . not only in Europe but throughout the world. Unlike many conference proceedings all the chapters con tained within this book have been subjected to rigorous peer review and several potential contributions were rejected during the editing process. Furthermore, most manuscripts underwent extensive editing to try and produce chapters of a uniform format and standard."

This series was established to create comprehensive treatises on specialized topics in developmental biology. Such volumes are especially vital in develop mental biology, since it is a very diverse field that receives contributions from a wide variety of disciplines. This series is a meeting-ground for the various practitioners of this science, facilitating an integration of heterogeneous infor mation on. specific topics. Each volume is intended to provide the conceptual basis for a comprehen sive understanding of its topic as well as an analysis of the key experiments upon which that understanding is based. The specialist in any aspect of devel opmental biology should understand the experimental background of the field and be able to place that body of information in context to ascertain where additional research would be fruitful. At that point, the creative process takes over, and new experiments are designed. This series is intended to be a vital link in that ongoing process of learning and discovery. If it facilitates schol arship, it will serve an important function."

During the past three decades, the cerebral vasculature and its role in blood-brain transport has been an increasingly active area of investigation and learning, particularly from an anatomical and physiological point of view. However, much less is known at the molecular and cellular level about the blood-brain barrier especially regarding the macromolecules responsible for transport, the roles played by vascular wall components (endothelial cell, pericyte, smooth muscle, basement membrane), and the mechanisms regulating brain vascular-specific protein expression and their molecular alterations during development and disease. Fundamental questions still unanswered include: What are the molecular constituents of brain endothelial cell tight junctions? What are the membrane proteins responsible for transport of specific substrates? What are the molecular signals that cause glucose transporter gene expression to be 20 to 100 times greater in brain endothelial cells in vivo than in vitro? What roles do pericytes, smooth muscle cells and basement membrane have in establishing or maintaining blood-brain transport characteristics? Are brain vascular transport systems responsible for edema following injury? Are transporter systems regulated via receptor-mediated events? Do hormones or neuromodulators regulate transporter expression? What is the molecular mechanism by which plasma proteins enter the extravascular space? Are transporters asymmetrically distributed between the luminal and abluminal endothelial cell membranes? Can prodrugs or pharmacologic agents be designed as substrate analogs and be delivered to the central nervous system via existing transporters or receptors? Can new and beneficial transporters be introduced into the brain vasculature?

The discovery of adenoviruses naturally induced a new interest in viruses of the human upper respiratory tract since previously unknown viruses infecting this portion of the human body had not been identified in 20 years, and their unique characteristics stimulated investigations into the biochemical events essential for replication of animal viruses. Indeed, the field of molecular virology has evolved during the period since their dis covery, and adenoviruses have played a major role in this development. The exciting discoveries made with adenoviruses have had such a pro found effect on knowledge in basic virology, molecular biology, viral ge netics, human and animal infections, and cell transformation that this seemed a propitious time to have some of the major contributors review this field. This volume pays tribute to the late Wallace Rowe, Robert Huebner, and Maurice Hilleman whose initial discoveries of adenoviruses have tremendously enriched virology. Harold S. Ginsberg vii Contents Chapter 1 An Overview 1 Harold S. Ginsberg Chapter 2 The Architecture of Adenoviruses M. V. Nermut I. Introduction ................................... . 5 II. Chemical and Physical Properties ................... . 6 III. Virus Capsid: Composition and Organization .......... . 7 A. Hexon ..................................... . 10 B. Penton .................................... . 12 C. Other Virus Polypeptides Associated with the Capsid 13 D. Organization of the Capsid ..................... . 14 IV. Virus Core .................................... . 15 A. Evidence for the Core Shell ..................... . 17 B. Organization of the DNA-Protein Complex (Nucleoc- sid) ....................................... . 18 C. Tentative Model of the Adenovirus Nucleocapsid ... . 22 V. Model of the Adenovirion ......................... . 29 32 References .......................................... ."

Biological control is the suppression of pest populations using predators, parasitoids and pathogens. Historically, biological control has largely been on a trial-and-error basis, and has failed more often than it has succeeded. However by developing theories based upon fundamental population principles and the biological characteristics of the pest and agent, we can gain a much better understanding of when and how to use biological control. This book gathers together recent theoretical developments and provides a balanced guide to the important issues that need to be considered in applying ecological theory to biological control. It will be a source of productive and stimulating thought for all those interested in pest management, theoretical ecology and population biology.

The integument plays an important role in the survival of meta zoans by separating and protecting them from a hostile environ ment. Its function ranges from protection against injury and in fection; partlcipation in the regulation of body temperature and water balance, to respiratory activity, monitoring of the environ ment and production of signals related to behaviour. All these result from specific structural, biochemical and physiological properties of intra-and extracellular components of the integu ment. Thus its characterization can be best accomplished by a multidisciplinary approach with authors specialized in different fields of science. This multi-author book, in two volumes, provides an up-to date survey of the literature. The first volume deals with the integument of invertebrates, the second with that of vertebrates, both organized primarily on a phylum basis. As the level of knowledge on the integument of phyla differs considerably, the information provided is correspondingly either limited or con densed. For some of the smaller groups of invertebrates little information is available, as often only a few electron micrographs are to be found in the literature; on the other hand, from the large body of knowledge existing for vertebrates, particularly for mammals, no complete overview can be provided, but publica tions giving access to further information have been reviewed critically."

MATERIALS AND METHODS ...330 RESULTS ...331 DISCUSSION ...333 REFERENCES ...335 SEcnON VI: THE MARATEA CONFERENCE ...337 PHOTO LAYOUT ...339 PARTICIPANTS ...341 INDEX ...*. **. ****************************** 345 xxv SECTION I Biochemistry and cell biology of serine proteases and serpins THROMBIN STRUCIURAL REGIONS IN DETERMINING BIOREGULATORY FUNCTIONS JOHN W. FENTON n Wadsworth Center for Laboratories & Research, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201, and Department of Physiology, The Albany Medical College of Union University, Albany, NY 12208, USA INTRODUCTION Thrombin (EC 3. 4. 21. 5) is the activation product of its blood-circulating or cellular-sequestered zymogen, prothrombin. Unlike the majority of enzymes and activated proteins of the blood-coagulation, fibrinolytic, and complement systems, thrombin has multiple bioregulatory functions, which are central in hemostasis, wound 1 6 healing, and perhaps developmental, as well as certain disease processes. - Hemostasis occurs at three levels, which are: i) plasma proteins (e. g. , coagulation factors V, VIII, and IX; proteins C and S, complement components), ii) blood cells (e. g. , platelets, monocytes, neutrophils), and iii) blood vessels (e. g. , endothelium, smooth muscles). Moreover, thrombin functions at all three levels of hemostasis, while the majority of cell types known are responsive to greater or lesser extents to thrombin. Thrombin furthermore functions both as a proteolytic enzyme with arginine- or lysine-directed specificities or as a nonenzymic activated protein 1 6 (hormone-like) involving receptor occupancy on certain cells.

There have been substantial developments in the methodologies available for the non-destructive and non-invasive measurement of body composition in animals. By bringing together in a single volume a mix of traditional and well-established analytical methods with more modern techniques, Body Composition Analysis: A Handbook of Non-destructive Methods provides a theoretical overview of different methodologies combined with practical advice on the use of these techniques. Methods covered include the use of destructive methods of analysis, body condition indices, isotope and gas dilution methods, total body electrical conductivity, bio-impedance analysis, ultrasound scanning and dual energy X-ray absorptiometry. Aimed at active research workers from advanced undergraduate level upwards, this book will be of particular interest to those working in the fields of animal ecology, conservation biology, animal nutrition and physiology.