The papers in this volume were presented at the Symposium on Cell Biology of the Uterus held December 12, 1986, on the NIH campus, Bethesda, MD. This was the first of a series of meetings that will be held in con junction with the annual meeting of the American Society for Cell Biology. The uterus is now recognized as an extremely complex organ whose nor mal function is orchestrated by a delicate procession of cellular and molecular events that investigators are beginning to unravel for the first time. Powerful new analytical methods and the tools of molecular biology are now providing exciting breakthroughs in our basic understanding of uterine structure and function. Thus, the program of this meeting was or ganized to cover recent developments in uterine cell biology including the mechanism of hormone action, control of gene expression by nuclear acceptor sites and nuclear receptors, role of growth factors, endometrial cell kine tics during the menstrual cycle, regulation of specific protein synthesis and secretion, decidual cell function, and the role of early pregnancy pro teins. The material presented in this volume is concerned not only with how hormones and growth factors prepare the endometrium for implantation of the blastocyst, but it also details the recent characterization and identification of specific marker proteins secreted in response to hormone action and early pregnancy."

This book is intended as a general text for undergraduates studying the manage ment of forest insect pests. It is divided into four parts: insects, ecology, manage ment, and practice. Part I, Insects, contains two chapters. The first is intended to provide an overview of the general attributes of insects. Recognizing that it is impossible to adequately treat such a diverse and complex group of organisms in such a short space, I have attempted to highlight those insectan characteristics that make them difficult animals to combat. I have also tried to expose the insects' weak points, those attributes that make them vulnerable to manipulation by human actions. Even so, this first chapter will seem inadequate and sketchy to many of my colleagues. Ideally, this book should be used in conjunction with a laboratory manual covering insect anatomy, physiology, biology, behavior, and classifica tion in much greater depth-in fact, this is how I organize my forest entomology course. It is hoped that this first chapter will provide nonentomologists with a general feel for the insects and with a broad understanding of their strengths and weaknesses, while Chapter 2 will provide a brief overview of the diverse insect fauna that attacks the various parts of forest trees and their products."

The function of the central nervous system as a coordinator and regulator of cellular processes in multicellular organisms is unequivocal. Until recently, however, the chemical evidence necessary for validating speculations on neurophysiological function in inverte brates has been lacking. In insects, because of their small size, heroic efforts were needed to collect the millions of tissues or organs necessary for isolation and identification of neurochemicals. With the spec tacular advances in physical and analytical technology within the last decade and with significant advances in radiochemical, radioimmunological and neurophysiological assays, researchers are, for the first time, able to handle microgram and nanogram quantities of complex biological substances. More recent developments in immunology promise to lower these levels further. It is not surprising that these new opportunities accelerated progress in insect neuroscience and that the time was right for a rapid and personal exchange of ideas and information on techniques. These considerations were the primary impetus for convening the International Conference on Insect Neurochemistry and Neurophysiology (ICINN) at the University of Maryland, College Park, MD, on August 1-3, 1983."

viii The final section consists of a chapter on development by Thomas Schoenfeld and Christiana Leonard and three chapters devoted to functions of individuals. These in clude biological rhythms by Lawrence Morin, energy balance by Katarina Borer, and the visual and somatosensory systems by Barbara Finlay and Claire Berian. I am extremely delighted and proud to have played a role in this work and I wish to thank each contributor, Plenum Press (especially Kirk Jensen), Jay Rosenblatt, and the Rutgers University Research Council. In addition, I extend my thanks and my love to my family-Marilyn, Matt, Paul, Scott, and my mother and father, who taught me to appreciate animals. Harold I. Siegel Newark, New Jersey Contents I. Origin 0/ the Hamster Chapter 1 History of the Capture and Domestication of the Syrian Golden Hamster (Mesocricetus auratus Waterhouse) MICHAEL R. MURPHY 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 2. The Early Years: Discovery and Description. . . . . . . . . . . . . . . . . . . . . . . . 4 3. On the Road to Jerusalem: Capture and Breeding ................... 7 4. The Invasion of America: Dissemination and Proliferation . . . . . . . . . . . . 13 . 5. New Genes on the Line: A Reconnaissance Expedition to Aleppo ....... 17 6. Conclusion ................................................. 18 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 . . . . . . . . . . . . . II. Reproductive Endocrinology Chapter 2 The Estrous Cycle ROBERT D. LISK 1. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 . . . . . . . . . . . . 2. Sexual Maturation-The First Estrous Cycle ....................... 24 2.1. The Ovary ............................................. 26 2.2. The Pituitary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 . . . . . . . . . . 2.3. The Brain ............................................. 31 3. Follicle Maturation and Ovulation ............................... 33 3.1. Time of Ovulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 . . . . . . . . . 3.2. Ovarian Steroids and Ovulation . . . . . . . . . . . . . . . . . . . . . . . 35 . . . . . ."

This volume is a compilation of the papers presented at a meeting that took place in April 1980 at the Mote Marine Laboratory, Sarasota, Florida. The meeting and this volume are outgrowths of two earlier international meetings on marine bio-acoustics that occurred in 1963 and 1966 (Tavolga 1964, 1967). The first meeting took place at the Lerner Marine Laboratory of the American Museum of Natural History, while the second meeting was at the American Museum itself, and was under the sponsorship of the Department of Animal Behavior. It is apparent that these two volumes have had immense impact on the current study of marine bio-acoustics, and particularly on fish audition. In a preliminary conference in Sarasota in 1979 we decided that it was time for another such meeting, to bring together as many as possible of the investigators interested in fish acoustics in order to assess the current state of our knowledge and predict directions for research for the next several years. Such a meeting appeared par ticularly timely, since over the past four or five years there have been many new studies that have provided new empirical and theoretical work on basic mechanisms of fish audition. Furthermore, it became evident, as we made up preliminary lists of possible participants, that few of the currently active workers were in the field back in 1966. In fact, of the current participants, only Drs."

The deposition of calcium-containing salts is a widespread phenomenon in both the plant and animal kingdoms. Its occurrence suggests a generalized biological adaptation to environments rich in calcium. Indeed, the Archaean ocean was rich in calcium carbonate, and traces of ancient organisms have been found in lime stones estimated to be about 2. 7 billion years old. The fundamental nature of biological calcification makes it a subject of interest not only to the student of calcium metabolism and skeletal physiology, but also to biologists in general. As in many areas of biological science recent progress in this field has been rapid, and no attempt was made here to cover all the biological systems in which calcifica tion is an important facet of the organisms' method of operation. Calcification is approached in this volume at the levels of the cellular sites and molecular mechan isms that are involved in this process. The ultrastructural and chemical features of the cells and their products which are associated with calcification are empha sized in several chapters. The editor, in inviting contributions from authors, in tended that collectively the chapters should convey a sense of the ubiquitous and essential nature of the role of calcification in several phyla of the plant and animal kingdoms. The researchers were biochemists, physical chemists, cell biologists and physiologists; some represented medicine and dentistry; all were interested in calcification.

"Calcareous algae and stromatolites" is shorthand for a wider array of organisms and fabrics that also includes calcified cyanobacteria, plus thrombolites and other microbial carbonates. Composition is the link: these are all important components of CaC0 sediments, from 3 Archaean to present and from the ocean floor to streams and lakes. It is hardly possible to examine limestones of any age without en- countering them. Simultaneously they are fossils, sediments, and en- vironmental indicators. It is the range of significance, coupled with the breadth of their distribution in time and space, which compels their study. Modern calcareous marine algae mainly include reds (corallines, squamariaceans, and the nemalialean Galaxaura) and greens (dasy- cladaleans, udoteaceans, halimedaceans). Blue-greens, of course, are cyanobacteria and not algae, and significantly, although they are largely responsible for Recent tidal flat stromatolites, they are not calcified in the same way that pre-Cenozoic marine blue-greens are. It is in the freshwater environment of calcareous streams and lakes that we find modern calcified cyanobacteria, and they are commonly associated with the only major group of non-marine calcareous algae, the charophytes. However, in the past, and especially in the Palaeozoic and Mesozoic, things look radically different. Mingling with the ancestors of the modern flora are distinct, and often problematic, organisms.

This volume represents the proceedings of a NATO Advanced Study Institute on "Molecular Aspects of Monooxygenases and Bioactivation of Toxic Compounds" which was held in ~e me (Izmir) Turkey, August 27 to September 7, 1989. The Institute emphasized the potential dangers of drugs, pollutants, pesticides, carcinogens, and both nutrient and non-nutrient food additives and their interactions at the molecular level. These xenobiotics are metabolized in phase I reactions by monooxygenases, primarly cytochrome P450-isozyme dependent, followed by phase II conjugation enzymes. The versatility of these enzymes is unique in biochemistry since they catalyze the metabolism of an almost limitless number of compounds through a diverse array of reactions. Although these metabolic sequences are usually detoxication mechanisms, in some cases the reactive intermediates are more mutagenic, more carcinogenic and ultimately more toxic than the parent compound. The Institute was devoted to recent scientific progress in the understanding of the biochemical and molecular properties of these enzymes and factors that influence the metabolism of chemicals to toxic and non-toxic compounds. Overview lectures focused on xenobiotic metabolism by cytochrome P450, the flavin-containing monooxygenase, cytochrome b5, epoxide hydrolase, glutathione S-transferase and UDP-glucuronosyl transferase. Several additional presentations stressed various aspects of cytochrome P450 mediated metabolism including: specific forms in the rabbit and insects; topology in membranes; posttranslational modification; control of constitutive forms in the rat; hormonal and xenobiotic regulation; prostanoid metabolism; endogenous substrate metabolism; interaction with estrogenic pesticides; pesticide bioactivation. The Institute also included talks on interactions between enzymes and on benzene metabolism and toxicity.

Mycobacteria is divided into two volumes. The first volume deals with the basic biology of mycobacteria. With its emphasis on the state of the art outlook, this volume includes taxonomy and molecular biology of mycobacteria, modern approaches for detection of mycobacteria, and immunology and immunization against tuberculosis. The second volume covers drug trestments for mycobacteria anad tuberculosis. It outlines trends of discovery and development of chemotherapy, starting from the mid-50's to present day uses of chemotherapy in treating AIDS, drug-resistant tuberculosis, and other non-tuberculosis mycobacterial diseases.

Post-translational modifications serve many different purposes in several cellular processes such as gene expression, protein folding and transport to appropriate cell compartment, protein-lipid and protein-protein interactions, enzyme regulation, signal transduction, cell proliferation and differentiation, protein stability, recycling and degradation. Although several-hundred different modifications are known, the significance of many of them remains unknown. The enormous versatility of the modifications which frequently alter the physico-chemical properties of the respective proteins represents an extraordinary challenge in understanding their physiological role. Since essential cellular functions are regulated by protein modifications, an improvement of current understanding of their meaning might allow new avenues to prevent and/or alleviate human and animal diseases.

The publication of this volume of The Viruses entitled The Togaviridae and Flaviviridae comes at an appropriate time. The structure and rep lication strategies of these viruses are now known to be sufficiently di verse to warrant the removal of flaviviruses from the Togaviridae family and establish them as an independent family. Flaviviridae have a special place in the history of virology. The prototype virus-yellow fever virus was the first virus to be identified as the cause of a human disease. Some of the history of this discovery is described in Chapter 1 of this volume; in Chapter 10 the complete sequence of the RNA genome of the virus is presented. This sequence not only defines the primary structure of the viral proteins, it also clarifies the mechanism of translation of the fla vivirus genome. Knowledge of the sequence of the structural proteins of these viruses represents an important step in the potential goal of using purified flavivirus glycoproteins as vaccines. Many of the chapters in this volume focus on the structure and replication of the Togaviridae. These viruses have provided valuable models for studies in cell biology, partic ularly with regard to the cotranslational and posttranslational steps re quired for the synthesis and localization of membrane glycoproteins. Fur thermore, Togaviridae have been pivotal in our growing understanding of how enveloped viruses enter and exit from cells. The broad outlines of the structure and gene expression of Togavir idae and Flaviviridae are known, but important questions remain."

It is perhaps obvious to any student of Biology that the discovery of chemical processes in whole organisms has usually preceded the elucidation of the compo nent steps. However, it is perhaps less obvious that the unravelling of the se quences in which those chemical steps occur in living matter, of the precise mechanisms involved, and of the manner in which they are regulated, would have been achieved neither by the study of intact plants and animals nor even of extracts derived from them. Our ability to understand the nature and regulation of metabolism rests on two main premises: the postulate that life processes can indeed be validly investigated with individual cells and cell-free extracts, and the thesis that there is an essential "unity in biochemistry" (as Kluyver put it, 60 years ago) that enables events in one organism to be legitimately studied in another. Of particular utility in this latter respect has been the use of cultures of single-celled organisms, growing in defined media-especially prokaryotes, such as Escherichia coli, and eukaryotes, such as Neurospora and Sac charomyces sp. , to which both biochemical and genetical techniques could be applied. It was, of course, Pasteur's observations of bacterial fermentations that first overthrew the belief that oxygen was essential for all energy-yielding pro cesses: his recognition that "La fermentation . . . . . c' est La vie sans air" laid the foundations of our knowledge of glycolysis.

since the earliest results on the linkage between ras activation and cell transformation appeared a vast amount of additional information has been generated which emphasizes the importance of ras-related genes in membrane trafficking, cell proliferation, differentiation and cancer. These advances led to the development of new strategies for the diagnosis, prognosis and prevention of cancer. Ras-related genes appear to be central to the mechanism of transformation by other oncogenes and therefore constitute a focal point upon which attempts to intervene in the process of carcinogenesis will be concentrated. The present volume contains the contributions to the NATO Advanced Research Workshop on the "The Superfamily of ras-Related Genes" held in Agia Pelagia, Heraklion, Crete, Greece, May 1991. At this meeting the leading researchers in this field carne together to discuss the most recent progress in analysis of biological function of the ras-related genes. As an organizer I feel that the Workshop was a success, and would like to thank all the participants and the contributors to this volume. I thank the NATO Scientific Affairs Division, Brussels, the Greek Ministry of Industry, Energy and Technology, the International Center for Cancer Research, the National Hellenic Research Foundation and the Medical School, University of Crete for sponsoring the Meeting.

During the past decade a significant international research effort has been directed towards understanding the composition and regulation of the preocular tear film. This effort has been motivated by the recognition that the tear film plays an essential role in maintaining corneal and conjunctival integrity, protecting against microbial challenge and preserving visual acuity. In addition, research has been stimulated by the knowledge that alteration or deficiency of the tear film, which occurs in countless individuals throughout the world, may lead to desiccation of the ocular surface, ulceration and perforation of the cornea, an increased incidence of infectious disease, and potentially, pronounced visual disability and blindness. 7 To promote further progress in this field of vision research, the International Conference on the Lacrimal Gland, Tear Film and Dry Eye Syndromes: Basic Science and Clinical Relevance was held in the Southampton Princess Resort in Bermuda from November 14 to 17, 1992. This meeting was designed to assess critically the current knowledge and 'state of the art' research on the structure and function of lacrimal tissue and tears in both health and disease. The goal of this conference was to provide an international exchange of information that would be of value to basic scientists involved in eye research, to physicians in the ophthalmological community, and to pharmaceutical companies with an interest in the treatment of lacrimal gland, tear film or ocular surface disorders (e. g. Sjogren's syndrome).

As the editors of the first book on the squirrel monkey prophesied in 1968, * there has been an incredible expansion in primate research during the past 16 years. Their projection that the squirrel monkey would play an increasingly important role in this research effort has also come to be true during the ensuing years. One inadvertent result of the rapid growth, however, is that it has become more and more difficult for investigators to keep track of new information, both in their own disciplines and in related fields. For scientists who study and use the squirrel monkey in research, this problem is particularly pronounced, because articles are often published in specialized and disparate journals. We felt that a new synthesis of the vast amount of information on Saimiri would resolve this problem and would provide an extremely valuable com panion volume to the first book. The idea grew out of a small symposium held at the IX Congress of the International Primatological Society in Atlanta, Geor gia, during August, 1982. Following the format of The Squirrel Monkey, ad ditional authors were invited to discuss advances in areas which had experi enced exceptional growth or to review basic information that would be of practical value to future researchers. Even with focused topics and synthetic reviews, the wealth of new data resulted in many long manuscripts. In response to the continuing problems with Saimiri nomenclature, Richard Thorington has provided us with a definitive statement on squirrel monkey taxonomy."

The pUrpOSE! of this conference was not to define the two areas that are being bound, which might be a well nigh impossible proposition. Rather, its focus was to concentrate on the mechanistic similarities between promotion and progression. Are the areas involved within the boundaries a continuum? Are these two simultaneous processes? Or are some of the affected cells in the stage of promotion when at the same time others have undergone irreversi ble changes tha. t position them in the stage of progression? Or are these two stages the same thing, but called by different names? To explore such concepts we assembled investigators with various back grounds and asked them to specifically address these and other questions about "The Boundaries", within the context of the session to which they con tributed. The conference lasted two and a half days, from Wednesday to Friday. There were at least four speakers per session with morning and after noon sessions each day, except on Friday when the meeting ended at noon. The first day, each speaker had 25 minutes to present a position, followed by five minutes of discussion. At the end of the session there were 40 or 50 minutes of exchange on all the issues examined. For the remaining days, there were 25 minutes of presentation and 15 minutes of discussion.

This book represents the proceedings of the NATO Advanced Study Institute held in Santa Flavia, Sicily from the 20 - 29th June, 1977. In addition to the review talks given by the Lecturers at the Institute it proved feasible for other topics to be splendidly reviewed. This has led to a much wider subject coverage than would otherwise have been possible. The discussion sessions which followed these review talks were extremely valuable and almost all the participants played an active role. Essentially all of the verbal contributions presented at this ASI were subsequently put into written format, which is why these proceedings are so extensive. ~hey do, however, provide an up-to-date summary of DNA synthesis in a wide variety of subjects with many of the remaining problems clearly expressed. The editing of these contributions has been essentially confined to alterations in style and presentation. We have taken some liberties in the re-organization of the papers into related sections. We express our thanks to those who helped organize the ASI and to the session conveners who attempted to confine and contain those who became too verbose. We are indebted to NATO, Scientific Affairs Division for the financial support that made this ASI possible. Finally, we express our gratitude to Miss Brenda Marriott. She typed all seventy five papers in this book, which was originally estimated to be less than half its present length and which just grew and grew. She deserves our special thanks.

This book launches a new continuing series in developmental biology. Any new publishing venture in science can only be justified if it satisfies a need and fulfills that need in an exemplary way. I believe that there is an acute need for comprehensive treatises on specialized topics in developmental biology-especially because this is a very diverse field that receives con tributions from a wide variety of disciplines. It is difficult to remain abreast of progress in multifaceted topics and to understand how the various aspects of a topic interrelate. The volumes that will comprise this series are designed to facilitate those processes. Each volume of this series will attempt to convey the current status of research and thought on a specific topic in development, place current research within historical perspective, and lay the intellectual groundwork for future research. These volumes are intended to provide an in-depth background on these topics for advanced undergraduates, graduate stu dents, and senior scientists. An investigator considering research in one of these areas will get an overview of the field as well as discover the status of recent research in the area."

Genetic Engineering, Volume 24 contains discussions of contemporary and relevant topics in genetics, including: -Gene silencing: principles and applications, -Integrins and the myocardium, -Plant virus gene vectors: biotechnology and applications in agriculture and medicine, -Novel approaches to controlling transcription, -Use of DNA polymorphisms in genetic mapping, -Application of FLP/FRT site-specific DNA recombination system in plants. This principles and methods approach to genetics and genetic engineering is essential reading for all academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in this continuously emerging field.

Lakes in the Balkans are numerous, but few of them have been studied, and even fewer on a long-term basis. This book is the first on a lake of the Balkans since the monograph on Lake Ohrid (1956). It is a synthesis of our present knowledge on the ecology and functioning of the lake and its resources, with an emphasis on its endemism and on the management needed to preserve its unique fauna and flora. It also contains original long-term studies on pelicans and fish. The target readership is well-educated lay people, conservationists (especially birdwatchers) and wetland scientists.

The NATO Advanced Study Institute on "Experimental Embryology in Aquatic Plant and Animal Organisms" was attended by more than 70 participants, including 15 invited main lecturers from 18 different countries. In accordance with the main purpose of the meeting, senior scientists, postdoctoral investigators and graduate students working in areas of descriptive and experimental embryology, classical, molecular and developmental biology, physiology and biochemistry etc. , were brought together for two weeks as a community with a strong common interest in "development"; that is, the multiple phenomena and mechanisms, in molecular, cellular, genetic and organismic terms, observed in the development of aquatic organisms. Initial concern that the great variety of biological models as well as of research subjects would harm the scientific quality and coherency of the course was unnecessary. It was exactly this breadth which made the Institute worthwhile for each of the participants. Since many of the "students" were younger scientists starting a career, it was the main goal of the course to offer a concise overview of selected system models of primarily aquatic organisms and to present and discuss research carried out in the past and in progress. Thus, each main speaker gave two in-depth lectures: one in which he presented an overview of "his" model and another dealing with current investigations.

''An exciting glance at key issues in contemporary hematopoiesis.'' -The Quarterly Review of Biology

Seventeen years after its initial description, nuclear factor-KB (NF-KB) endures as one of the most studied transcription factors. NF-KB has attracted widespread interest based on the variety of stimuli that activate it, the diverse genes and bio logical responses that it controls, the striking evolutionary conservation of struc ture and function among species, and its involvement in a variety of human diseases. The biochemical basis by which several stimuli converge to activate NF-KB has been largely elucidated during recent years. While first discovered as a key regulatory factor of the immune system, NF-KB is now recognized as an important player in the functioning of many organs and cell types. The ongoing examination of NF-KB signaling has revealed its ever expanding role in immune and inflammatory responses, but also in cancer and development. For this reason, numerous efforts are underway to develop safe inhibitors of NF-KB to be used in the treatment of both chronic and acute disease situations. The present book is the first to review and synthesize our knowledge of this interesting transcription factor. As such, the choice of subjects to review was daunting. To set the stage, an introductory chapter on activators and target genes, as well as the role they play in several responses, has been included."

Among the fishes. a remarkably wide range ofbiological adaptations to diverse habitats has evolved. As weIl as living in the conventional habitats of lakes. ponds. rivers. rock pools and the open sea. fish have solved the problems of life in deserts. in the deep sea. in the cold Antarctic. and in warm waters of high alkalinity or of low oxygen. Along with these adaptations. we find the most impressive specializations of morphology. physiology and behaviour. For example we can marvel at the high-speed swimming of the marlins. sailfish and warm-blooded tunas. air breathing in catfish and lungfish. parental care in the mouth-brooding cichlids and viviparity in many sharks and toothcarps. Moreover. fish are of considerable importance of the survival of the human species in the form ofnutritious and delicious food ofnumerous kinds. Rational exploitation and management of our global stocks of fishes must rely upon a detailed and precise insight of their biology. The Chapman and Hall Fish and Fisheries Series aims to present timely volumes reviewing important aspects of fish biology. Most volumes will be of interest to research workers in biology. zoology. ecology and physiology. but an additional aim is for the books to be accessible to a wide spectrum of non specialist readers ranging from undergraduates and postgraduates to those with an interest in industrial and commercial aspects of fish and fisheries."

Chemical Anatomy of the Zebrafish Retina is an excellent reference, providing excellent images and insight into one of the most increasingly important model systems in neurobiology today. Papers include a visually stunning molecular phenotype atlas and an exhaustive treatment of the neurochemical anatomy of the zebrafish retina as determined by immunocytochemistry.