This text attempts to introduce the molecular biology of cell membranes to students and professionals of diverse backgrounds. Although several membrane biology books are available, they do not integrate recent knowledge gained using modern molecular tools with more traditional membrane topics. Molecular techniques, such as cDNA cloning and x-ray diffraction, have provided fresh insights into cell membrane structure and function. The great excitement today, which I attempt to convey in this book, is that molecular details are beginning to merge with physiological responses. In other words, we are beginning to understand precisely how membranes work. This textbook is appropriate for upper-level undergraduate or beginning graduate students. Readers should have previous or concurrent coursework in biochemistry; prior studies in elementary physiology would be helpful. I have found that the presentation of topics in this book is appropriate for students of biology, biochemistry, biophysics and physiology, chemistry, and medicine. This book will be useful in courses focusing on membranes and as a supplementary text in biochemistry courses. Professionals will also find this to be a useful resource book for their personal libraries.

Epigenetic modifications comprise heritable gene expression changes that occur without alteration of the DNA sequence and 'co-act' with genetic factors to shape development processes and evolutionary trajectories. Multicellular organisms receive different types of environmental stimuli/stresses that trigger epigenetic modifications during development. These environmentally driven mechanisms represent an underlying cause of phenotypic diversity, especially in metazoans. This book aims to present some of the latest epigenetic insights into the development of metazoans (including humans) as an intersection between their ecology and evolution.

This book provides salient information on all aspects of influenza/flu viruses affecting animals and humans. It specifically reviews the properties and replication of influenza viruses; their evolution and emergence; epidemiology; role of migratory birds in disease transmission; clinical signs in humans, animals, and poultry; pathogenesis and pathogenicity; public health importance and potential threats; diagnosis; prevention and control measures; and pandemic preparedness. Influenza/flu viruses evolve continuously and jump species causing epidemics as well as pandemics in both human and animals. During the past 150 years, various strains of influenza virus like the Spanish flu, Asian flu, Hong Kong flu, bird flu, and swine flu were responsible for high mortality in humans as well as birds. High mutation rates, antigenic shifts, drifts, reassortment phenomena, and the development of antiviral drug resistance all contribute to ineffective chemotherapy and vaccines against influenza viruses. Due to their devastating nature, high zoonotic implications, and high mortality in humans and poultry, they have a severe impact on the socioeconomic status of countries. Disease awareness, rapid and accurate diagnosis, surveillance, strict biosecurity, timely adoption of appropriate preventive and control measures, and pandemic preparedness are crucial to help in decreasing virus transmission, thus reducing clinical cases, deaths, and pandemic threats.

This book has arisen from the Second European Meeting on Bacterial Genetics and Ecology (Bageco-2) held at the University of Wales, College of Cardiff which we organised on 11-12 April 1989. The meeting was attended by some 60 participants from eight European countries and was made possible by partial financial support from the Commission of the European Communities (CEC) and Imperial Chem ical Industries (UK) Ltd. The meeting was organised to discuss modern developments in the genetics of bacteria in aquatic and terrestrial habitats. It followed on from, and complemented, the first meeting of this series organised by Jean-Pierre Gratia in Brussels during April 1987 which concentrated more on medical and epidemiological issues. ! The next meeting will be organised by Michel J. Gauthier in 1991 at Nice, France. If you have been fired with enthusiasm for ecological bacterial genetics after having read this book, and want to attend the next meeting but did not hear about the one in Cardiff, you should write to Dr Gauthier to be put on the address list. A lot is now known about bacterial genetics at the physiological, biochemical and molecular level, and bacterial ecology has developed rapidly over the last 20 years. However, until very recently, few researchers have crossed the divide and linked these two specialisms.

This volume reviews the historical roots and theoretical foundations of biological systematics in an approachable text. The author outlines the structure and main tasks of systematics. Conceptual history is characterized as a succession of scientific revolutions. The philosophical foundations of systematic research are briefly reviewed as well as the structure and content of taxonomic theories. Most important research programs in systematics are outlined. The book includes analysis of the principal problematic issues as "scientific puzzles" in systematics. This volume is intended for professional taxonomists, biologists of various specialties, students, as well as all those interested in the history and theory of biology and natural sciences. Key Features Considers the conceptual history of systematics as the framework of evolutionary epistemology Builds a hierarchically organized quasi-axiomatic system of taxonomic theory Contends that more reductionist taxonomic concepts are less objective Supports taxonomic pluralism by non-classic philosophy of science as a normal condition of systematics Documents that "taxonomic puzzles" result from conflict between monistic and pluralistic attitudes Related Titles de Queiroz, K. et al., eds. Phylonyms: A Companion to the PhyloCode (ISBN 978-1-1383-3293-5) Sigwart, J. D. What Species Mean: A User's Guide to the Units of Biodiversity (ISBN 978-1-4987-9937-9) Rieppel, O. Phylogenetic Systematics: Haeckel to Hennig (ISBN 978-1-4987-5488-0) Wilkins, J. S. Species: The Evolution of the Idea, 2nd ed. (ISBN 978-1-1380-5574-2)

Entomopathogenic bacteria (Bacillus thuringiensis and B. sphaericus) are increasingly used as biopesticides to control larval insect populations which are either agricultural or forestry pests and to reduce those which as adults are vectors of severe human diseases. This new book, the first since 1993 to address all aspects of entomopathogenic bacteria, provides undergraduate and graduate students as well as research scientists with a complete, modern view of this important group of bacteria. The authors, chosen for their sustained contributions to the field, cover both fundamental and applied research in this area. The main topics include bacterial ecology and taxonomy, toxin diversity, activity and mode of action, regulation and environment of the genes, safety and ecotoxicology, production and field application of the bacteria, and outbreaks of resistant populations. The book concludes with the most recent data obtained on transgenic biotechnology and addresses environmental impact issues.

Clonal animals, that is those that are genetically identical, are of great importance in biology. The supposed evolutionary advantages of sexual versus asexual reproduction are one of the central paradoxes of current evolutionary theory. The evolved strategy of asexual reproduction includes a large number of diverse species in many different groups, for example aphids, guppies, planktonic rotifers and others. This book draws together many aspects of the functional biology of clonal animals, from their taxonomy and feeding ecology to their life-histories and evolutionary biology. The mechanism of somatic division and parthenogenesis and the benefits of cloning to a variety of Metazoa are examined. Together with a consideration of obligate cloning and modular organisms, this book should be a valuable reference for biologists with an interest in genetics, ecology and evolutionary biology.

In recent years, parasitologists have learned much about the way in which parasite biochemistry differs from that of free-living organisms. Inside the host, factors such as temperature, oxygen tension, carbon dioxide concentration and pH have important consequences for the biochemistry and physiology of the parasitic organism. Strong selection pressure therefore operates at all stages of a parasite's life cycle. The parasite's adaptational response to this pressure is the subject of this book. Under the headings "The nature of parasite adaptation", "Energy metabolism", "Digestion and uptake of nutrients", "Host immunity and parasite adaptation", "Biochemical adaptation and the magic bullet" and "Biochemical variation in parasites", present knowledge of parasite metabolism and how it reflects adaptation to particular environments is surveyed. The ways in which these adaptations may have developed during their evolution - with reference to the modern evolution of strain variations and the development of resistance to antiparasitic compounds - are explored, together with immunological aspects of the host-parasite relationship and potential metabolic targets for chemotherapy. This book should be of use to all those involved and interested in the biochemistry of parasites and its relation to their evolution, ecology, adaptation and variation. Those aspects of parasite metabolism that are attacked by anti-parasitic drugs are described, making this a useful book for those involved in the pharmaceutical industry. This book should be of interest to parasitologists and comparative biochemists.

A 29-volume set which contains all Charles Darwin's published works. Darwin was one of the most influential figures of the 19th century. His work remains a central subject of study in the history of ideas, the history of science, zoology, botany, geology and evolution.

The present book is a monograph about two groups of hypotrichous ciliates, namely the Amphisiellidae and the Trachelostylidae. It is the third of six volumes which - view the Hypotricha, one of the three major taxa of the spirotrichs. The first volume is about the Oxytrichidae, a rather large group, many species of which have 18 highly characteristically arranged frontal-ventral-transverse cirri and, much more importantly, a comparatively complex dorsal ciliature due to (oxytrichid) fragmen- tion of dorsal kineties during cell division (Berger 1999). The second volume deals with the Urostyloidea, which are characterised by a zigzag-arrangement of the ventral cirri (Berger 2006). Although this pattern is often very impressive, it is a relatively simple feature originating by a more or less distinct increase of the number of frontal-ventral-transverse cirri anlagen. These anlagen produce cirral pairs which are serially arranged in non-dividing specimens. Some - ers are likely astonished that the monograph on urostyloids does not include Urol- tus, a group of tailed species, which also have a distinct zigzagging cirral pattern. However, morphological and molecular data indicate that the zigzag pattern of U- leptus evolved independently, that is, convergently to that of the urostyloids. Thus, Uroleptus was excluded from the urostyloid review. A zigzag pattern is also known from some oxytrichids, for example, Neokeronopsis, Territricha, Pattersoniella, showing that this pattern evolved several times independently (Berger 1999, 2006, Foissner et al. 2004).

Volumes 21 to 29 in a 29-volume set which contain all Charles Darwin's published works. Darwin was one of the most influential figures of the 19th century whose work remains a central subject of study in the history of ideas, the history of science, zoology, botany, geology and evolution.

The IACUC Administrator's Guide to Animal Program Management supports IACUC administrators who assist with developing, managing, and overseeing a program of animal care and animal use. It provides many options and possibilities for specific operational practices (e.g., how to build a well-functioning IACUC, what a functional protocol template looks like) to satisfy regulatory requirements. The material provided is a compilation of several years of Best Practices (BP) meetings among IACUC administrators across the country. The BP meetings included representatives from the NIH/OLAW, AAALAC, and the USDA, whose presence and dialogue assured the BP discussion met or exceeded all regulatory or accreditation minimum standards. BP meeting attendees from private, public, governmental, and academic organizations have helped to shape and develop the information offered herein. It is through the insight of several hundred colleagues-their successes as well as their failures-that the authors have distilled suggestions and considerations for your local animal care and use program. This handbook complements other useful references and manuals regarding programmatic function-it is not intended to replace them. The primary difference you will find is the transparent and open nature of describing processes that have been time tested and proven to help you and your organization satisfy the regulatory requirements.

Volumes 11 to 20 in a 29-volume set which contain all Charles Darwin's published works. Darwin was one of the most influential figures of the 19th century whose work remains a central subject of study in the history of ideas, the history of science, zoology, botany, geology and evolution.

An interdisciplinary challenge to assumptions about animals and animality deeply embedded in our own ways of thought, exposing sensitive and largely unexplored aspects of the understanding of our common humanity.

Ever since the discovery of fossil remains of extinct animals associated with flint implements, bones and other animal remains have been providing invaluable information to the archaeologist. In the last 20 years many archaeologists and zoologists have taken to studying such "archaeofaunal" remains, and the science of "zoo-archaeology" has come into being. What was the nature of the environment in which our ancestors lived? In which season were sites occupied? When did our earliest ancestors start to hunt big game, and how efficient were they as hunters? Were early humans responsible for the extinction of so many species of large mammals 10-20,000 years ago? When, where and why were certain animals first domesticated? When did milking and horse-riding begin? Did the Romans influence our eating habits? What were sanitary conditions like in medieval England? And could the terrible pestilence which afflicted the English in the seventh century AD have been plague? These are some of the questions dealt with in this book. The book also describes the nature and development of bones and teeth, and some of the methods used in zoo-archaeology.

Receptor specific antibodies are excellent probes for a wide range of biological investigations on receptor structure and function. The hybridoma technology (Kohler and Milstein, 1975) has inevitably had a major impact on this field with most of the better known receptors now identified with monoclonal antibodies. This volume of the Receptors and Recognition series provides reviews of recent developments in this field and emphasizes in particular the new opportunities afforded by the judicious application of monoclonal reagents. It is assumed that most readers will be familiar with the now fairly routine methods of cell fusion, hybridoma cloning and selection for producing monoclonal antibodies and so few details of the basic technical procedures are described. Several good reviews on this topic are however available (see Galfre and Milstein, 1981; Goding, 1980; Yelton and Scharf, 1981; McMichael and Fabre, 1982). By no means all vertebrate receptor species are discussed here; omissions include antibodies to low density lipoprotein receptors (Beisiegel et al. , 1981; Kita et al. , 1981), prolactin and growth hormone receptors (Friesen etal. , 1982; Simpson et al. , 1983) and the hepatocyte asialoglycoprotein receptor (Schwartz et al. , 1981; Harford et al. , 1982). Nevertheless the coverage is comprehensive and critical and the individual chapters provided illustrate vividly the rapid progress being made.

Sunday Times Bestseller 'Passionate and well-researched' Tatler 'A must-read' Independent A social history of Labradors, and how they have become the world's most beloved dogs, by writer, presenter and long-time dog lover Ben Fogle. Labradors are the most popular breed of dog in the world. Not only a great family companion, they also excel at hunting, tracking, retrieving, guiding and rescuing. But where did the breed originally come from? How did it develop? When did black, yellow and chocolate Labradors first appear? Did they really all come from Labrador in Canada and are they really all related to just one dog? In this first history of the Labrador, Ben Fogle goes in search of what makes Labradors so special. Their extraordinary companionship, intelligence, work ethic and loyalty is captured by Ben as he weaves the story of the breed into his own story of his beloved Inca. Ben visits Canada, discovers hair-raising stories of early Labrador exploits and uncovers stories of RNIB Labradors and Labradors at war, Labradors as working dogs and every other manifestation of the Labrador's character. Exploring their origin, early characteristics, their use as gun dogs, as therapy dogs, as police dogs, as search and rescue dogs and last - and absolutely not least - as family pets, Ben tells the story of a dog breed which has captured our imagination and love for hundreds of years.

The suppression of apoptosis by the IGF system is critical for normal cell development, proliferation, differentiation and motility. Aberrations in IGF signalling mechanisms contribute to cell transformation, tumour progression and metastasis. Many questions remain to be answered as to how exactly the IGF system mediates its effects both in normal and tumour cells and how the IGF-1R interacting proteins and downstream signalling cascades are regulated. The importance of the IGF system is underscored by the significant interest in the development of anti-IGF therapies for IGF sensitive cancers. Future developments in cancer therapy are likely to focus on methods to target these therapies to diseased but not normal cells. 14. Acknowledgements We would like to thank Kurt Tidmore for preparing the illustrations. The Health Research Board of Ireland and Science Foundation Ireland are grateful acknowledged for funding. 15. References Adamo M., Roberts C. T., Jr. and LeRoith D. (1992) How distinct are the insulin and insul- like growth factor I signalling systems? Biofactors 3, 151-7. Adams T. E., Epa V. C., Garrett T. P. and Ward C. W. (2000) Structure and function of the type 1 insulin-like growth factor receptor. Cell Mol Life Sci 57, 1050-93. Adler V., Polotskaya A., Wagner F. and Kraft A. S. (1992) Affinity-purified c-Jun ami- terminal protein kinase requires serine/threonine phosphorylation for activity. J Biol Chem 267, 17001-5.

Animal cell technology has undergone a rapid transformation over the last decade from a research tool and highly specialised technology to a central resource for innovation in pharmaceutical research and development. These proceedings of the 14th Meeting of the European Society for Animal Cell Technology (Vilamoura, Portugal, May 1996) bring up to date the historical perspective of animal cell technology for the benefit of society, `From Vaccines to Genetic Medicine', and will charter this vital technology for the years to come. Strong contributions are grouped in the traditional ESACT areas of 'Cell and Physiology Engineering' dealing with cell state, including genetics, and its environment, and 'Animal Cell Process Engineering' covering integration of bioreaction with bioseparation coupled with on-line monitoring to improve protein production and consistency. Extensive coverage of metabolic engineering on synthesis, folding, assembly, transiting and secretion is dealt with in the session on 'Recombinant Proteins: Biosynthesis and Bioprocessing'. Two traditional but expanding areas of animal cell technology relevance are highlighted in the broad sessions of 'Animal Cells as Tools for Discovery and Testing' and 'Animal Cell Vaccines: Present and Future'. Two sessions finally cover the more recent domains of animal cell technology work - 'Tissue Engineering and Biomedical Devices' and 'Cells and Vectors for Genetic Medicine' - where one can foresee a very bright future.

This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy. The volume provides molecular approaches to be used within an "integrative taxonomy" framework, combining a range of nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany, etc.), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Molecular Plant Taxonomy: Methods and Protocols, Second Edition is an ideal guide for researchers seeking a better understanding of evolutionary processes, at species and population level, through molecular techniques.