One of the most striking and persistent ways humans dominate Earth is by changing land-cover as we settle a region. Much of our ecological understanding about this process comes from studies of birds, yet the existing literature is scattered, mostly decades old, and rarely synthesized or standardized. The twenty-seven contributions authored by leaders in the fields of avian and urban ecology present a unique summary of current research on birds in settled environments ranging from wildlands to exurban, rural to urban. Ecologists, land managers, wildlife managers, evolutionary ecologists, urban planners, landscape architects, and conservation biologists will find our information useful because we address the conservation and evolutionary implications of urban life from an ecological and planning perspective. Graduate students in these fields also will find the volume to be a useful summary and synthesis of current research, extant literature, and prescriptions for future work. All interested in human-driven land-cover changes will benefit from a perusal of this book because we present high altitude photographs of each study area.

If I had to nominate an area of food production in which science has played a major role in addressing product quality to meet market needs I would not pass by the intimate rela tionship of cereaI chemistry with cereaI plant breeding programs. In Australia, cereaI chemistry and product quality labs ha ve long been associated with wheat and barley breeding programs. Grain quality characteristics have been principal factors determining registration of new cultivars. This has not been without pain in Australia. On the one hand some cultivars with promising yield and agronomic characteristics have been rejected on the basis of quality characteristics, and for a period our breeders imposed selection regimes based on yield which resulted in declining quality characteristics. In the end the market provides the critic al signals. For many years Australia held a commanding market position on the basis of a single quality image, initiaHy based on bulked wheat of fair/average quality (FAQ). Later this was improved by segregation into four broad classes* based around Australian Standard White (ASW). This is no longer a viable marketing strategy. We were probably a little slow in rec ognising the mosaic of present day wheat markets, but now have up to 18 different grades available. Around the world wheat is a grain with many end uses. Its use in bread is expanding."

Despite the many advances made in the diagnosis and therapy of cancer, the mortality rate is still about half that of the incidence rate. However, the odds are not evenly distributed. Prognosis for some cancers is good, but for others, few patients will survive 12 months. This latter group of cancers is characterised by a proclivity to disseminate malignant cells in the host organ. The degree of surgery possible may be limited by the critical nature of the organ, and chemotherapy and radiotherapy are of palliative value only. In some cases systemic metastases occur, but in other cases, failure to achieve local control results in death. First among these cancers are the high grade brain tumours, astrocytoma 3,4 and glioblastoma multiforme. Local control of these tumours should lead to cure. Other cancers melanoma metastatic to the brain, for which a useful palliative therapy is not yet available, and pancreatic cancer for which localised control at an early stage could bring about improved prognosis. Patients with these cancers have little grounds for hope. Our primary objective is to reverse this situation with Neutron Capture Therapy (NCT). The purpose of this fourth symposium is to hasten the day whereby patients with these cancers can reasonably hope for substantial remissions. The first symposium on NCT was held in Boston in 1983, followed by Tokyo in 1985 and Bremen, Germany in 1988.

The anther tapetum, present in all land plants, is a highly specialized, transient tissue surrounding the (micro-)spores and/or pollen grains during their development. Any tapetum malfunction causes male sterility. The exact knowledge of tapetum form and function therefore is indispensable not only for basic research, but also and especially in plant breeding and plant genetics. In fourteen contributions by reknown experts, a comprehensive account of the various characters and functions of the tapetum is provided, covering the areas of cytology, cytophysiology, biochemistry, tapetum development and function.

As one of the most quantitative of ecological subdisciplines, resource competition is an important, central area of ecology. Recently research into this area has increased dramatically and resource competition models have become more complex. The characterisation of this phenomenon is therefore the aim of this book. Resource Competition seeks to identify the unifying principles emerging from experimental and theoretical approaches as well as the differences between organisms, illustrating that greater knowledge of resource competition will benefit human and environmental welfare. This book will serve as an indispensable guide to ecologists, evolutionary biologists and environmental managers, and all those interested in resource competition as an emerging discipline.

Following the demise of the MAFF's Perma some leaflets to fill outstanding gaps. The nent Leaflet Series in 1985, it was suggested content of three leaflets has been altered more that the final editions of the crop pest advisory extensively. Thus, Chafer grubs (Chapter 32) leaflets should be produced in a bound volume now incorporates material from Leaflet 449, for the benefit of future agricultural entomolo Japanese beetle, which has been omitted from gists and others interested in crop pests. This this collection as this pest has not become idea originated outside MAFF but was enthusi established in Europe. Nematodes on straw astically supported by entomologists in MAFF, berry (Chapter 81) has been enlarged by the the agricultural research institutes and the uni addition of further information on free-living versities. As editor of the leaflets from 1965 nematodes from the plant pathology leaflet on until 1985, I offered to undertake the task of Soil-borne virus diseases of fruit plants. The compilation and editing, and this offer was joint plant pathology/entomology leaflet on accepted. Raspberry cane blight and midge blight has To prevent the book from becoming quickly been included, but the information has been outdated, the sections on control measures mainly restricted to raspberry cane midge and have been redrafted by the Advisory Entomol the title changed accordingly.

This volume contains the papers presented in the International Summer Course on "Cirrhosis, Hyperammonemia and Hepatic Encephalopathy," which was one of the prestigious Summer Course, of the Complutense University of Madrid held in EI Escorial, Spain, during August 10-14, 1992. Liver cirrhosis is one of the main causes of death in western countries. In addition there is a series of liver dysfunctions including fulminant hepatic failure, Reye's syndrome and congenital defects of urea cycle enzymes that could lead to hepatic encephalopathy, coma and death. As a consequence of impaired liver function, the ability to detoxify ammonia by its incorporation into urea is diminshed, resulting in increased ammonia levels in blood and brain. Hyperammonemia is considered one of the main factors in the mediation of hepatic encephalopathy and the classical clinical treatments are directed towards reducing blood ammonia levels. A part of the book is therefore devoted to the study of certain aspects of ammonia metabolism such as the regulation of the urea cycle, the main mechanism of ammonia detoxification in mammals, which is located mainly in the liver. The metabolism of ammonia in other tissues, including brain, is also presented, as well as the effects of hyperammonemia on brain metabolism and function and on brain microtubules. The control of cerebral protein breakdown is reviewed. The classical and some recently proposed clinical treatments as well as nutritional considerations in the management of patients with liver failure are also discussed.

Advances in DNA sequencing and phylogenetic inference have created powerful methods to investigate many dangerous human viruses. The Molecular Epidemiology Of Viruses provides a comprehensive introduction to the use of genetic methods in molecular epidemiology and in-depth examples of analyses from many viruses. This book is of interest to researchers in the fields of infectious disease, virology, microbiology, evolutionary biology, epidemiology and molecular biology as well as anyone interested in tracking the spread of disease.

Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to uncover their abilities for industrial and medical purposes. The goal of animal cell technology includes clonal expansion of differentiated cells with useful abilities, optimization of their culture conditions on the industrial scale, modulation of their ability in order efficiently to produce medically and pharmaceutically important proteins, and application of animal cells to gene therapy and formation of artificial organs. This Volume gives the readers a complete review of the present state of the art in Japan, a country where this field is well advanced, as well as in Asia, Europe and the United States. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, biochemical engineers and those in other disciplines related to animal cell culture, working in academic environments as well as in the biotechnology and pharmaceutical industries.

The 5th International Conference on Aspartic Proteinases was held on September 19 through 24, 1993, at Naito Museum of Pharmaceutical Science and Industry, Kawashima cho, Gifu Prefecture, Japan, about 15 miles northwest of Nagoya City. About 100 scientists attended the conference, including 52 from 14 countries outside Japan, and 32 papers were presented by invited speakers, and 58 papers as posters. The purpose of this conference was to present and discuss new information on the structure, function, and biology, and related topics, including biomedical implications, of aspartic proteinases, and this book is a collec tion of nearly all the papers presented at the meeting. Aspartic proteinases belong to one of the four major classes of proteinases, the others being serine, cysteine, and metalloproteinases, and are so called since they have two catalytic aspartic acid residues in common in their active sites. Most of them are optimally active at acidic pH, hence the long-used name "acid proteinases," which, indeed, was the major title of the first conference of this series. However, some of them are active at around neutral pH, indicating their physiological roles in a wider range of pH than hitherto considered.

Recent years have seen tremendous advances in our understanding of the molecular mechanism of platelet activation. All aspects of signal transduction in platelets from the identification of surface receptors, G proteins, phospholipases, protein kinases and phosphatases, intracellular receptors for inositol phosphates, the Ca2+ regulatory machinery, cytoskeletal constituents to the control mechanism employing cyclic nucleotides has seen an explosion of information regarding their importance and for each constituent in the family of molecules to which they belong. This information has been of interest to researchers across a wide spectrum of disciplines including biochemists, pharmacologists, cell biologists and clinicians. In April 1992 an International Symposium bearing the name of this volume was organised at the Thrombosis Research Institute to bring together scientists from across the world whose common interest was the study of platelet activation and its regulation. We were particularly encouraged by the positive response from our speakers and the participants, their detailed contributions and the very lively discussions that took place throughout the two days of the symposium. Almost every aspect of signal transduction in human platelets was represented. Of the invited speakers twelve were from Europe (including the U. K. ), eight from North America and one from Japan. This volume is a compilation of chapters submitted by the speakers and represents a concise but informative picture of the present knowledge of the mechanisms of platelet activation and control.

Over the last decade enormous advances 10 our understandmg of the functionmg of "tomata have been made Today, the overall pattern of events 10 stomatal functloOlng IS generally under~tood although some of the fmer details of the mechaOlsm remam to be eluCIdated However, the mechaOlsm whereby changmg CO levels wlthm a leaf control stom- 2 atal movements remam~ one of the biggest puzzles The excellent text PhYSlOlogy of Stomata, by Professors H Meldner and T A Mamfleld, I~ over 12 years old and mevltably sections of It are now outdated Although numerou~ flOe review,> on vanous aspects of stomata have been wntten 10 the meantime, none could be conSidered as complete, compact appraisals of the subject dnd thus to have super- seded the Meldner and Mansfield book Now IS therefore an appropnate time to review the statu~ of our knowledge about stomata This book encompas~es all a~pect~ of the subject except the phySICal processes mvolved 10 the exchange of gases between a leaf and ItS envI- ronment ThiS topIC IS dealt With very adequately 10 a vanety of other books and reVlew~, and the subject matter, With mmor differences of opmlon, remams much the '>ame today as It was perhaps 20 years ago A descnption of how to measure stomatal apertures and the use of porometers has been restncted to a fmal chapter whICh also deals with expenments which can be carned out by students Agam, there are

Surprising though it seems, the world faces almost as great a threat today from arthropod-borne diseases as it did in the heady days of the 1950s when global eradication of such diseases by eliminating their vectors with synthetic insecticides, particularly DDT, seemed a real possibility. Malaria, for example, still causes tremendous morbidity and mortality throughout the world, especially in Africa. Knowledge of the biology of insect and arachnid disease vectors is arguably more important now than it has ever been. Biological research directed at the development of better methods of control becomes even more important in the light of the partial failure of many control schemes that are based on insecticide- although not all is gloom, since basic biological studies have contributed enormously to the outstanding success of international control programmes such as the vast Onchocerciasis Control Programme in West Africa. It is a sine qua non for proper understanding of the epidemiology and successful vector control of any human disease transmitted by an arthropod that all concerned with the problem - medical entomologist, parasitologist, field technician - have a good basic understanding of the arthropod's biology. Knowledge will be needed not only of its direct relationship to any parasite or pathogen that it transmits but also of its structure, its life history and its behaviour - in short, its natural history. Above all, it will be necessary to be sure that it is correctly identified.

It is now well recognised that many environments considered by man to be extreme are colonised by micro-organisms which are specifically adapted to these ecological niches. These organisms not only survive but actively grow under such conditions. A diverse range of bacteria, cyanobacteria, algae and yeasts has now been isolated from these habitats which are extreme in terms oftemperature, pH, salinity and pressure as well as species which are resistant to radiation and toxic chemicals. Whilst originally considered to be mere 'scientific curiosities', it is now generally accepted that many have con siderable biotechnological and commercial significance. Recently the term 'extremophile' has been used to describe these organisms. Over the past twenty years extensive studies of the ecology, physiology, taxonomy and molecular biology of these micro-organisms have been undertaken. These have resulted in a complete reassessment of our concept ofmicrobial evolution. The identification ofthe Archaeobacteria as the third kingdom ofliving organisms has given considerable impetus to extremophile research and is presenting many new challenges."

After volume 33, this book series was replaced by the journal "Evolutionary Biology." Please visit www.springer.com/11692 for further information. This volume continues bringing to readers the findings of eminent evolutionary biologists and paleobiologists. Among the topics discussed in this book are the origin of the dermal skeleton in conodont chordates, patterns of nucleotide substitution and codon usage in plasmid DNA evolution, a model to explain phenotype stability in functional systems, and inter-island speciation of Hawaiian biota.

The Seventh International Congress of Mucosal Immunology held in Prague, the beautiful old capital of The Czech Republic, 16-20 August 1992, was the first to be sponsored by the Society for Mucosal Immunology, and was the largest since their inception 20 years earlier in Birmingham, Alabama. It was attended by 624 participants who gave 538 presentations, more than 10 times the numbers of the first meeting; these proceedings contain 354 papers that were submitted for publication. The political events in Europe that made it possible to hold this Congress in Prague also allowed for the first time the participation of large numbers of scientists from Eastern Europe, as weil as from Asia, and the organizers were truly gratified by this happy circumstance. It is now clear not only that mucosal immunityencompasses the huge area of mucosal surfaces and most physiological organ systems, but also that mucosal immunology extends over the whole global surface and all continents! The sheer size of the Congress and number of manuscripts unfortunately entailed some unexpected problems in editing and assembling the proceedings, partly due to the diversity of linguistic styles not represented at earlier meetings, and we apologize to the authors who have patiently awaited the publication of their contributions.

Genomic instability is a major threat to living organisms. To counteract the damaging effects posed by endogenous and environmental agents, such as chemicals or radiation, micro-organisms devote several percent of their genome to encode proteins that function in the repair and recombination of DNA. For many years, a relatively small group of scientists have carefully delineated the molecular mechanisms of these repair processes, using the simplest model systems available, namely Escherichia coli and Saccharomyces cerevisiae. These studies, which until recently had only moderate impact outside of the field, now provide the cornerstone for exciting new research into analogous processes in human cells. The reason for this is the revelation that the biochemical pathways for the accurate replication, repair and recombination of DNA have been conserved through evolution. New research shows that human cells use DNA repair mechanisms that are analogous to those found in bacteria to overcome the damaging effects of environmental mutagens and carcinogens. Of particular significance is the observation that certain cancers are caused by defects in DNA repair enzymes. For example, the human inherited non-polyposis colon cancer is now known to be caused by defects in the enzymes that repair DNA mismatches. Because much detailed information has been accumulated in studies of analogous mismatch repair proteins from bacteria, rapid progress can now be made.

Kevin Marshall is a hard act to follow. Volume 13 of Advances in Microbial Ecology has been produced by a new editorial board, and we, the members of that board, are delighted to have the opportunity to pay tribute to Kevin's achievements. In his time as Series Editor, the quality of the chapters submitted and the range of subject matter covered have ensured an expanding and more stimulated readership. This represents a considerable achievement, given the growth in the number of review volumes and the increasing tendency for journals to publish review articles. The achievement was reached not only through metic ulous attention to quality and detail but also by providing a forum for the expression of views, information, and results that would stimulate discussion. Advances in Microbial Ecology will continue to provide such a focus, although, because of the frequency of publication, it would not be practicable to introduce a "reply" or "comment" section. Although we do not deliberately aim to provide a forum for controversy, we encourage speculation based on sound scientific arguments. In addition, we would like to encourage authors to offer chapters for consideration. In the past, the volumes have largely comprised invited chapters. With the best will in the world, an editorial board of four cannot claim adequate coverage of such a vast and rapidly developing research area. We would there fore welcome submission of outline plans for chapters, which should be sent to the Editor.

The book is the first annotated reader to focus specifically on the discipline of zoosemiotics. Zoosemiotics can be defined today as the study of signification, communication and representation within and across animal species. The name for the field was proposed in 1963 by the American semiotician Thomas A. Sebeok. He also established the framework for the paradigm by finding and tightening connections to predecessors, describing terminology, developing methodology and setting directions for possible future studies. The volume includes a wide selection of original texts accompanied by editorial introductions. An extensive opening introduction discusses the place of zoosemiotics among other sciences as well as its inner dimensions; the understanding of the concept of communication in zoosemiotics, the heritage of biologist Jakob v. Uexkull; contemporary developments in zoosemiotics and other issues. Chapter introductions discuss the background of the authors and selected texts, as well as other relevant texts. The selected texts cover a wide range of topics, such as semiotic constitution of nature, cognitive capabilities of animals, typology of animal expression and many other issues. The roots of zoosemiotics can be traced back to the works of David Hume and John Locke. Great emphasis is placed on the heritage of Thomas A. Sebeok, and a total of four of his essays are included. The Reader also includes influential studies in animal communication (honey bee dance language, vervet monkey alarm calls) as well as theory elaborations by Gregory Bateson and others. The reader concludes with a section dedicated to contemporary research. Readings in Zoosemiotics is intended as a primary source of information about zoosemiotics, and also provides additional readings for students of cognitive ethology and animal communication studies.

Investigation of the structure and function of biological molecules through spectroscopic methods is a field rich in revealing, clever techniques and demanding experiments. It is most gratifying to see that the basic concepts are applied to more and more complex systems, making feasible the study of the behaviour of whole systems in relation to molecular disturbances. The analytical potential of spectroscopy and spectroscopic imaging enables species identification of bacteria and tissue recognition. Clear opportunities for in vivo applications become apparent in the medical field. The methods developed in biophysics start to generate spin-off in the direction of biotechnology, where in previous years we have seen this happen for biochemical techniques. New directions are manifest. Tools are being developed to investigate the behaviour of single molecules in interaction with their environment. Individual interactions can now be investigated and individual molecules in complexes can be visualized. Processes that were previously unobservable as a result of ensemble averaging can now be investigated on a single molecule level. Completely new information with regard to molecular behaviour is obtained in this way. The insights amaze us and the prospect that this development will continue is exciting. The 8th European Conference on the Spectroscopy of Biological Molecules is proud to have contributed to the dissemination of these new directions. This proceedings book is an appropriate reflection of the progress obtained so far in the spectroscopy of biological molecules.

P. Doherty...VB Introduction D. Dobbelaere and D. McKeever...IX Theileria Development and Host Cell Invasion Michael K. Shaw...1 Genomic Polymorphism, Sexual Recombination and Molecular Epidemiology af Theileria Parva R. Bishop, D. Geysen, R. Skilton, D. Odongo, V. Nene, B. Allsopp, S. Mbogo, P. Spooner and S. Morzaria...23 Epidemiological Significance of Strain-Specific Immunity to Theileria Parva D. J. McKeever and W. I. Morrison...41 Virulence and Attenuation in Theileria Annulata R. Adamson and R. Hall...55 Theileria Survival Strategies and Host Cell Transformation V. T. Heussler...69 Genomics of Theileria Parva V. Nene, R. Bishop, J. Quackenbush, M. Pertea, S. L. Salzberg, E. Taracha, S. Morzaria, C. M. Fraser and M. Gardner...85 Non-Transforming Theileria Parasites of Ruminants C. Sugimoto and K. Fujisaki 93 Index...107 FOREWORD The apicomplexan protozoal parasites continue to provide major challenges for human and animal health. While most of us have some familiarity with the ravages of malaria, there is relatively little awareness of diseases caused by parasites of the Theileria species. The reason is that these tick-borne organisms are problematic only in cattle and small ruminants. This does not mean, however, that the various manifestations of Theileriosis are of little interest to those concerned principally with human health. The economic loss and diminished food production associated with East Coast Fever (ECF, caused by T. parva) continues to be a major problem in East Africa.

Biochemistry And Genetics of RecQ-Helicases provides a background into the role of helicases in general and RecQ helicases specifically in DNA repair. Helicases- enzymes which break down hydrogen bonds between nucleic acid strands in a nucleoside triphosphate-dependent manner-are ubiquitous in biology, participating in processes as diverse as replication, repair, recombination, transcription, and translation. The RecQ-family helicases are a group of helicases which have important roles in the maintenance of genomic stability in many organisms. In humans, mutations in three RecQ-family helicases lead to disease. This book thoroughly examines these helicases. Mutations in the BLM gene lead to Bloom syndrome, a disorder characterized by a susceptibility to many types of cancer. Mutations in the WRN gene cause Werner syndrome, a disease which in some respects resembles premature aging. Finally, mutations in a newly characterized RecQ-family member, RECQ4, may lead to the very rare recessive disorder Rothmund-Thomson syndrome, a condition characterized by developmental abnormalities and some aging-like manifestations. This book is intended for any researchers invested in these particular disorders, or with a general interest in DNA.

Marine biological science is now studied at the molecular level and although research scientists depend on information gained using molecular techniques, there is no book explaining the philosophy of this approach. Molecular Approaches to the Study of the Ocean introduces the reasons why molecular technology is such a powerful tool in the study of the oceans, describing the types of techniques that can be used, why they are useful and gives examples of their application. Molecular biological techniques allow phylogenetic relationships to be explored in a manner that no macroscopic method can; although the book deals with organisms near the base of the marine food web, the ideas can be used in studies of macroorganisms as well as those in freshwater environments.

Molecular and Cell Biology of Human Diseases reviews the status of research on a range of sexually transmitted diseases whose incidence has paralleled the increase in HIV infection and examines the ways in which new methods are influencing current practice and are likely to shape future management.

The articles in this volume represent papers delivered by invited speakers at the 7th International Symposium on the Immunobiology of Proteins and Peptides. In addition, a few of the abstracts submitted by participants were scheduled for minisymposia and some of the authors, whose presentations were judged by the Scientific Council to be of high quality, were invited to submit papers for publication in this volume. This symposium was established in 1976 for the purpose of bringing together, once every two or three years, active investigators in the forefront of contemporary immunology, to present their findings and discuss their significance in the light of current concepts and to identify important new directions of investigation. The founding of the symposium was stimulated by the achievement of major breakthroughs in the understanding of the immune recognition of proteins and peptides. We believed that these breakthroughs will lead to the creation of a new generation of peptide reagents which should have enormous potential in biological, therapeutic, and basic applications. This anticipated explosion has in fact since occurred and many applications of these peptides are now being realized. The seventh symposium focused on immune responses that have undesirable effects on the host, hence we named them unwanted immune responses. Two major aspects of unwanted immune responses were discussed at the symposium: Allergy and Autoimmunity.