Fishermen, marine aquarists, biologists studying seashore and coastal waters, and those involved in trading shellfish and even restaurateurs are aware of the great diversity of crustaceans inhabiting the seas around the British Isles, Northern Europe and the Mediterranean. Crayfishes, Lobsters and Crabs of Europe will enable the reader to identify 42 crustacean species of commercial importance found in these regions during coastal explorations, fishing trips, displayed in public aquaria or available in restaurants, including selected freshwater crayfishes, deep-sea species and some imported species. The book also includes sections on the gross internal and external structure of these Crustacea, their life histories, classification and nomenclature. The book is of interest to students of marine biology and researchers in fisheries science.

This compendium is the result of the FEMS Workshop on "Rapid Diagnosis of Mycoplasmas" which I organized and which took place in Jerusalem, Israel, August 11-23, 1991. The first week's sessions were held at a resort on the outskirts of Jerusalem and consisted of lectures and discussions. This part was modelled along the lines of the Gordon Conference in the USA, i.e., in an intimate atmo sphere in which everyone could mix and exchange ideas, and was very benefi cial. About 100 scientists from around the world attended the first week. Dur ing the first week, the biology, molecular biology and pathophysiology of myco plasmas, as well as all the main diagnostic methods were covered, including both conventional and the newer technologies. The session on mycoplasmas in the human urogenital tracts was held in conjunction with the Israel Society for the Study and Prevention of Sexually Transmitted Disease. The second week was a laboratory session and was held at the Hebrew University-Hadassah Medical School campus in Ein Karem, Jerusalem. All ex periments were conducted by eminent specialists in their field. The lab session had 36 participants from 19 countries who used the most modern techniques for the diagnosis of mycoplasmas in medicine, veterinary medicine and agri culture. The efficacy of several commercial kits were also tested at this time. I want to again thank everyone who helped and supported this work shop, as well as the authors of the various chapters.

This is a handbook of artificial diets for rearing insects, mites and spiders. Its origin goes back to my association with Dr H. L. House at the Research Institute of the Canadian Department of Agriculture at Belleville, Ontario. In 1967, Dr House published a bulletin Artificial Diets for Insects: A Compilation of References with Abstracts. We jointly updated this work in 1971 to include papers published from 1967-1970 and to take special note of the use of antimicrobial agents. In 1970 I left Belleville to join the Department of Scientific and Industrial Research in New Zealand where I continued to catalogue insect diets, and in 1972 compiled a Bibliography on Artificial Diets for Insects and Mites covering the period of 1900-1970. This was followed in 1974 by another publication entitled Artificial Diets for Insects containing insect diets published during May 1970-December 1972. These publications laid the foundations for the present volume. The aim of this book is to give complete but concise coverage of exi- ing knowledge of insect dietetics. It brings together in one volume the most complete survey of artificial diets for insects, mites and spiders to date. I hope its content will aid all those concerned with insect rearing programmes. I would like to thank the Department of Scientific and Industrial Research for permission to publish this book. I am particularly grateful to Dr J. M. Hoy, Director of the Entomology Division, for his encouragement.

This volume includes papers presented at the Third Annual Computation and Neural Systems meeting (CNS*94) held in Monterey California, July 21 - July 26, 1994. This collection includes 71 of the more than 100 papers presented at this year's meeting. Acceptance for meeting presentation was based on the peer review of preliminary papers by at least two referees. The papers in this volume were submitted in final form after the meeting. As represented by this volume, CNS meetings continue to expand in quality, size and breadth of focus as increasing numbers of neuroscientists are taking a computational approach to understanding nervous system function. The CNS meetings are intended to showcase the best of current research in computational neuroscience. As such the meeting is fundamentally focused on understanding the relationship between the structure of neIVOUS systems and their function. What is clear from the continued expansion of the CNS meetings is that computational approaches are increasingly being applied at all levels of neurobiological analysis. in an ever growing number of experimental preparations. and neural subsystems. Thus. experimental subjects range from crickets to primates; sensory systems range from vision to electroreception; experimental approaches range from realistic models of ion channels to the analysis of the information content of spike trains. For this reason, the eNS meetings represent an opportunity for computational neurobiologists to consider their research results in a much broader context than is usually possible.

This book contains the proceedings of the first meeting on invertebrate immunity ever sponsored as a summer research conference by the Federation of American Societies for Experimental Biology (FASEB). The conference was held in Copper Mountain, CO from July 11-16, 1999. It was a an extension of a New York Academy of Sciences meeting entitled "Primordial Immunity: Foundations for the Vertebrate Immune System" held on May 2-5,1993 at the Marine Biological Laboratories in Woods Hole, MA. The proceedings of that meeting were published in The Annals of the New York Academy of Sciences (volume 712). At that meeting all the attendes agreed that this type of conference (a relatively small focused gathering) allowed for participation by investigators at all levels of their careers. We further agreed that we should search for a forum that would allow this meeting to continue. The FASEB Summer Research Conference was an excellent vehicle for this type of meeting. Furthermore, this year's participants decided to continue this meeting as a regularly scheduled FASEB sponsored event. This was a unique conference in the sense that it focused upon mechanisms of development and defense in protostome and deuterostome invertebrates and lower vertebrates. There was a strong emphasis on evolutionary cell biology, phylogenetic inferences and the evolution of recognition and regulatory systems.

Research in Antarctica in the past two decades has fundamentally changed our perceptions of the southern continent. This volume describes typical terrestrial environments of the maritime and continental Antarctic. Life and chemical processes are restricted to small ranges of ambient temperature, availability of water and nutrients. This is reflected not only in life processes, but also in those of weathering and pedogenesis. The volume focuses on interactions between plants, animals and soils. It includes aspects of climate change, soil development and biology, as well as above- and below-ground results of interdisciplinary research projects combining data from botany, zoology, microbiology, pedology, and soil ecology.

A wide range of microbiologists, molecular biologists, and molecular evolutionary biologists will find this new volume of singular interest. It summarizes the present knowledge about the structure and stability of microbial genomes, and reviews the techniques used to analyze and fingerprint them. Maps of approximately thirty important microbes, along with articles on the construction and relevant features of the maps are included. The volume is not intended as a complete compendium of all information on microbial genomes, but rather focuses on approaches, methods and good examples of the analysis of small genomes.

W. French Anderson, M.D. The publication of this book comes at an opportune time for the young field of human gene therapy. After a decade of long struggle at the laboratory bench and many long hours under the harsh lights of the federal review process, gene therapy has emerged as a legitimate scientific discipline. It is now time to move away from the period of questioning whether gene therapy will be a useful part of the physician armamentarium to begin to actively teach the concepts and practices that make gene therapy a reality. This book is a comprehensive collection of chapters that describe the basic biology and potential application of viruses as gene transfer reagents. It is not a coincidence that a modified virus was the reagent used in the first human gene therapy trials. Viruses have evolved with the human species (and most likely with all forms of life) to be the masters of gene transfer.

From July 28 to August 3, 1991, an International Meeting on the REGULATION OF CHLOROPLAST BIOGENESIS was held at the capsis Beach Hotel in Aghia Pelaghia, on the island of crete, Greece. The Meeting (Advanced Research Workshop-Lecture Course) was co-sponsored by NATO, FEBS and IUB, and was held under the auspices of the International society for Chloro plast Development, the Greek Ministry of Industry, Research and Technol ogy, and the National Center for Scientific Research "Demokritos". The Meeting focused on recent advances in the field of chloroplast biogenesis and the regulatory mechanisms underlined, and brought together over 120 experts and students of the field from 22 countries. The subject of chloroplast biogenesis has experienced great progress in recent years mainly thanks to the application of Molecular Biology techniques and methodology. New findings that emerge gradually unravel the regulatory mechanisms involved in the assembly, stabilization and growth of the photosynthetic units in thylakoids, the signal transduction chain leading from photoreception to gene expression, the transport of nuclear-coded proteins into stroma-soluble supramolecular enzyme complexes as well as thylakoid-bound supramolecular complexes, involved in light-energy transduction. It was the aim of this meeting to bring together experts and students coming from diverse disciplines (ranging from Botany and plant physiology to Molecular Biology, Biophysics and Biotechnology), to discuss the recent advances in the field so that thorough exchange of ideas and working hypotheses would be achieved.

A there it is! guide to insects of medical and public health concern, mainly in the tropics. Each chapter covers identification, life cycle and habits of the causative stage and its medical/public health significance.

Honeybees are as small as flies or as large as hornets, nesting in nar row cavities of trees and rocks or in the open on large limbs of trees 30 m above ground. They occur in tropical zones and in the forests of the Ural mountains, they survive seven months of winter and even longer periods of drought and heat. Historically, they lived through a extended time of stagnation in the tropics from the mid-Tertiary, but then experienced an explosive evolution during the Pleistocene, re sulting in the conquest of huge new territories and the origin of two dozen subspecies in Apis mellifera. This vast geographic and ecologic diversification of the genus Apis was accompanied by a rich morphological variation, less on the level of species than at the lowest rank, the subspecies level. Variation being exclusively of a quantitative kind at this first step of speciation, tradi tional descriptive methods of systematics proved to be unsatisfactory, and honeybee taxonomy finally ended up in a confusing multitude of inadequately described units. Effective methods of morphometric-sta tistical analysis of honeybee popUlations, centered on limited areas, have been developed during the last decades. Only the numerical characterization of the populations, together with the description of behavior, shows the true geographic variability and will end current generalizations and convenient stereotypes."

The 11th International Conference on Lymphoid Tissues and Immune Reactions was held in Spa-Liege (Belgium), from 4 to 8 July 1993. The regular devotees refer to these conferences as the "Germinal Centre Conferences or GCC". In the 1960s, the germinal centres were the subject of such considerable study and speculation that a group of dynamic people decided to devote an international conference centered on that topic. This led to the fIrst GCC organized in Bern in 1966. Following the success of this initial meeting, further sessions have been organized at regular intervals and, over the years, the scope of the GCC has been broadened. Nowadays, the GCC conferences are dedicated to in vivo immunology and deal mainly with cellular, functional and molecular aspects of the lymphoid system. The credo of these conferences is "in vivo veritas," implying that the sole investigation of components (like molecules or cell populations) only gives a partial truth. Ultimately, the components have to be explored in their global context in order to see how they interact with other parts and how they are integrated as a whole to ensure the homeostasis of the immune system. In 1966, the GCC lasted 3 days and included 57 contributions "which were discussed at length. " The present conference lasted 4 days and accommodated 2 honorary lectures, 80 free papers and 209 poster presentations. Out of those presentations, a selection was performed by the Chairpersons to compose the present volume.

From May 3-7,1997, the NATO Advanced Research Workshop on 'Biological Electron Transfer Chains' was organized in Tomar, Portugal. In the application for support the choice of the topic was justified as follows: " Until recently efforts] have concentrated on the study of the structure and function of individual redox enzymes and proteins. Enough information is now available to make a start with the study of biological electron transfer (E1) at the next higher level of organization, that of the complete ET chain." The interest in the workshop was high: the majority of participants had registered before the workshop was formally announced, which illustrates the popularity of the topic within the biochemical and biophysical communities. The present volume contains a number of reports based on the lectures presented by the key speakers during the meeting. The workshop dealt with the following three themes: a) Electron transfer, which is the subject of Chapter 1. The analysis of ET at the molecular level is still fundamental for an understanding of how ET chains operate in vivo. After 40 years of research the contours of the subject are becoming clear now. b) Bacterial redox chains. This is the subject of Chapter 2. Its contents show how complicated these chains can be, often involving a number of gene clusters. Our understanding of the regulatory aspects and control mechanisms of these chains is only in its beginning.

Sound formulation is a vital aspect of microbial products used to protect plants from pests and diseases and to improve plant performance. Formulation of Microbial Biopesticides is an in-depth treatment of this vitally important subject. Written by experts and carefully edited, this important title brings together a huge wealth of information for the first time within the covers of one book. The book is broadly divided into five sections, covering principles of formulation, organisms with peroral and contact modes of action, organisms with the power of search, and future trends. Each section contains comprehensive chapters written by internationally acknowledged experts in the areas covered; the book also includes three very useful appendices, cataloguing formulation additives, spray application criteria and terminology. This outstanding book is a vitally important reference work for anyone involved in the formulation of microbial biopesticides and should find a place on the shelves of agriculture and plant scientists, microbiologists and entomologists working in academic and commercial agrochemical situations, and in the libraries of all research establishments and companies where this exciting subject is researched, studied or taught.

Molecular and Cell Biology of Human Diseases reviews the status of research on a range of sexually transmitted diseases whose incidence has paralleled the increase in HIV infection and examines the ways in which new methods are influencing current practice and are likely to shape future management.

Animal cell technology is a newly growing discipline of cell biology which aims not only to understand structure, function and behavior of differentiated animal cells but also to uncover their ability useful for industrial and medical purpose. The goal of animal cell technology includes clonal expansion of differentiated cells with useful ability, optimization of their culturing in industrial scale, modulation of their ability for production of pharmaceutical proteins and monoclonal antibodies, and newly application to gene therapy and organ culture. The last seven Annual Meetings of the Japanese Association for Animal Cell Technology (JAACT) had attracted increasing number of participants. At the Eighth Meeting (JAACT'95) held in Iizuka from November 6 through 10, 1995. Before this Meeting, we were all shocked by the sudden death of a founder of JAACT, the late Prof. Hiroki Murakami in February of this year. But we had more than 90 participants from outside of Japan and 170 from Japan in this Meeting. The editors express their sincere gratitude to all researchers who joined the meeting, to the organizers of the Symposium Sessions, to members of the organizing committee who dedicated themselves in assuring the Meeting's success in the absence of Prof. H. Murakami, and the graduates and undergraduates students of Kyushu University and Kyushu Institute of Technology who supported management of the Meeting. We also thank the Japanese Bioindustry Association and Fukuoka Science & Technology Foundation for the financial support.

This volume contains the papers presented in the International Summer Course on "Cirrhosis, Hyperammonemia and Hepatic Encephalopathy," which was one of the prestigious Summer Course, of the Complutense University of Madrid held in EI Escorial, Spain, during August 10-14, 1992. Liver cirrhosis is one of the main causes of death in western countries. In addition there is a series of liver dysfunctions including fulminant hepatic failure, Reye's syndrome and congenital defects of urea cycle enzymes that could lead to hepatic encephalopathy, coma and death. As a consequence of impaired liver function, the ability to detoxify ammonia by its incorporation into urea is diminshed, resulting in increased ammonia levels in blood and brain. Hyperammonemia is considered one of the main factors in the mediation of hepatic encephalopathy and the classical clinical treatments are directed towards reducing blood ammonia levels. A part of the book is therefore devoted to the study of certain aspects of ammonia metabolism such as the regulation of the urea cycle, the main mechanism of ammonia detoxification in mammals, which is located mainly in the liver. The metabolism of ammonia in other tissues, including brain, is also presented, as well as the effects of hyperammonemia on brain metabolism and function and on brain microtubules. The control of cerebral protein breakdown is reviewed. The classical and some recently proposed clinical treatments as well as nutritional considerations in the management of patients with liver failure are also discussed.

Following the demise of the MAFF's Perma some leaflets to fill outstanding gaps. The nent Leaflet Series in 1985, it was suggested content of three leaflets has been altered more that the final editions of the crop pest advisory extensively. Thus, Chafer grubs (Chapter 32) leaflets should be produced in a bound volume now incorporates material from Leaflet 449, for the benefit of future agricultural entomolo Japanese beetle, which has been omitted from gists and others interested in crop pests. This this collection as this pest has not become idea originated outside MAFF but was enthusi established in Europe. Nematodes on straw astically supported by entomologists in MAFF, berry (Chapter 81) has been enlarged by the the agricultural research institutes and the uni addition of further information on free-living versities. As editor of the leaflets from 1965 nematodes from the plant pathology leaflet on until 1985, I offered to undertake the task of Soil-borne virus diseases of fruit plants. The compilation and editing, and this offer was joint plant pathology/entomology leaflet on accepted. Raspberry cane blight and midge blight has To prevent the book from becoming quickly been included, but the information has been outdated, the sections on control measures mainly restricted to raspberry cane midge and have been redrafted by the Advisory Entomol the title changed accordingly.

I first became involved in research into primate behavior and ecology in 1968, over 40 years ago, driven by a quest for a better understanding of the natural context of primate evolution. At that time, it was virtually unknown that primates can exploit exudates as a major food source. I was certainly unaware of this myself. By good fortune, I was awarded a postdoctoral grant to work on lemurs with Jean-Jacques Petter in the general ecology division of the Museum National d'Histoire Naturelle in Brunoy, France. This provided the launching-pad for my first field study of lesser mouse lemurs in Madagascar, during which I gained my initial inklings of exudate feeding. It was also in Brunoy that I met up with Pierre Charles- Dominique, who introduced me to pioneering observations of exudate feeding he had made during his field study of five lorisiform species in Gabon. This opened my eyes to a key feeding adaptation that has now been reported for at least 69 primate species in 12 families (Smith, Chap. 3) - almost 20% of extant primate species. So exudativory is now firmly established as a dietary category for p- mates, alongside the long-recognized classes of faunivory (including insectivory), frugivory, and folivory. Soon after I encountered Charles-Dominique, he published the first synthetic account of his Gabon field study in a French language journal (Charles-Dominique 1971).

Kevin Marshall is a hard act to follow. Volume 13 of Advances in Microbial Ecology has been produced by a new editorial board, and we, the members of that board, are delighted to have the opportunity to pay tribute to Kevin's achievements. In his time as Series Editor, the quality of the chapters submitted and the range of subject matter covered have ensured an expanding and more stimulated readership. This represents a considerable achievement, given the growth in the number of review volumes and the increasing tendency for journals to publish review articles. The achievement was reached not only through metic ulous attention to quality and detail but also by providing a forum for the expression of views, information, and results that would stimulate discussion. Advances in Microbial Ecology will continue to provide such a focus, although, because of the frequency of publication, it would not be practicable to introduce a "reply" or "comment" section. Although we do not deliberately aim to provide a forum for controversy, we encourage speculation based on sound scientific arguments. In addition, we would like to encourage authors to offer chapters for consideration. In the past, the volumes have largely comprised invited chapters. With the best will in the world, an editorial board of four cannot claim adequate coverage of such a vast and rapidly developing research area. We would there fore welcome submission of outline plans for chapters, which should be sent to the Editor.

Marine biological science is now studied at the molecular level and although research scientists depend on information gained using molecular techniques, there is no book explaining the philosophy of this approach. Molecular Approaches to the Study of the Ocean introduces the reasons why molecular technology is such a powerful tool in the study of the oceans, describing the types of techniques that can be used, why they are useful and gives examples of their application. Molecular biological techniques allow phylogenetic relationships to be explored in a manner that no macroscopic method can; although the book deals with organisms near the base of the marine food web, the ideas can be used in studies of macroorganisms as well as those in freshwater environments.

During recent years the subject of extreme environments and extremophiles has become a central topic in modern Biology. The capability of some microorganisms to withstand, and often prefer, the harsh conditions found in such environments is helping to define the physicho-chemicallimits of life and in consequence its essential nature. Halophiles are one of the most representative types of extremophiles, requiring high concentrations of inorganic salts, mostly sodium chloride, to grow and survive. They inhabit hypersaline environments, the distribution and abundance of which dur ing geological eras are attested by the vast amounts of evaporite rocks present in the Earth crust and by their role in the generation of petroleum deposits. The corditions of high osmolarity and ionic strength that are concomitant with concentrated salt solutions challenge the stability of lipid bilayers and the structure of proteins forcing halophilic microbes to develop specialized molecules and physiological me;;hanisms to cope with this environmental stress. Even so, halophilism is a widespread trait in the microbial world. All the major groups of eucaryotic microbes, two groups of archaeobacteria and most phylogenetic branches of eubacteria have halophilic representatives. Therefore, the study of halophilic microorganisms is indeed a highly heterogeneous and extense topic. The present volume contains the contributions to the FEMS-NATO Advanced Research Workshop on "General and Applied Aspects of Halophilic Microorganisms" held at Alicante, Spain, September 17-22, 1989.

The advantages of the baculovirus system are rooted in the properties of the virus and the host (insect, or cell lines derived from it). During the normal infection cycle, two forms of the virus are produced: an early budded virus (BY) form (Kost et al. , 2000), in which the viral DNA and structural proteins are surrounded by membrane derived from the infected cell; and a late occluded form (occlusion-derived virus, ODy), consisting of enveloped viral cores which are embedded in a crystal matrix of viral proteins. The principal component of the matrix is the abundantly expressed protein polyhedrin. The budded virus rapidly spreads the infection from cell to cell within the insect host, resulting ultimately in the complete liquefaction of the host, and release of occluded virus into the environment. The occluded form protects the released virus, allowing it to survive for long periods in the environment until ingested by another host. In the alkaline environment ofthe insect gut, the protective protein matrix is removed, and the life cycle is repeated. In insect cell cultures, only the BV form of baculovirus is required, and the polyhedrin gene may be replaced with the gene for the recombinant protein. An additional benefit of replacing or deleting polyhedrin is that it effectively makes the virus unable to survive outside the laboratory, an advantage in terms of environmental safety. The system is intrinsically safe to animals, being unable to replicate in species other than a limited range of insects.

Despite the many advances made in the diagnosis and therapy of cancer, the mortality rate is still about half that of the incidence rate. However, the odds are not evenly distributed. Prognosis for some cancers is good, but for others, few patients will survive 12 months. This latter group of cancers is characterised by a proclivity to disseminate malignant cells in the host organ. The degree of surgery possible may be limited by the critical nature of the organ, and chemotherapy and radiotherapy are of palliative value only. In some cases systemic metastases occur, but in other cases, failure to achieve local control results in death. First among these cancers are the high grade brain tumours, astrocytoma 3,4 and glioblastoma multiforme. Local control of these tumours should lead to cure. Other cancers melanoma metastatic to the brain, for which a useful palliative therapy is not yet available, and pancreatic cancer for which localised control at an early stage could bring about improved prognosis. Patients with these cancers have little grounds for hope. Our primary objective is to reverse this situation with Neutron Capture Therapy (NCT). The purpose of this fourth symposium is to hasten the day whereby patients with these cancers can reasonably hope for substantial remissions. The first symposium on NCT was held in Boston in 1983, followed by Tokyo in 1985 and Bremen, Germany in 1988.

The Sixth International Workshop on the Enzymology and Molecular Biology of Carbonyl Metabolism was held outside of Dublin, Ireland at the end of June, 1992. Prof. Keith Tipton, Chairman of the Biochemistry Department at Trinity College, kindly agreed to host the meeting. On behalf of all of us who attended I wish to extend our sincere thanks to the whole Tipton family for making us feel so welcome in Ireland. It has been a decade since the frrst workshop was held in Bern, Switzerland. The scope of the meetings reflected somewhat the changes that have occurred in biochemistry during the past decade. At the first meeting primarily enzymes and their properties were discussed. At this last meeting many of the talks centered on gene regulation as well as more traditional aspects of enzymology and metabolism. During the past decade site directed mutagenesis to probe for the active site of an enzyme has become part of traditional enzymology; this was virtually unheard of at our frrst meeting. Many of the presenters now used this tool to study some aspect of structure and function of one of the three carbonyl metabolizing enzymes.