Since the publication of the pioneering first edition of Chemical Genomics and Proteomics more than seven years ago, the area of chemical genomics has rapidly expanded and diversified to numerous novel methods and subdisciplines, such as chemical glycomics and lipidomics. This second edition has been updated to uniquely reflect this interdisciplinary feature as well as the remarkable developments that have occurred. The new edition also covers innovative applications from cell biology to drug discovery to, more recently, clinical diagnostics and medical practice, which utilize the concepts of chemical genomics. The text provides an overview of the strategies and methodologies of chemical genomics, focusing on emerging technologies and recent applications in the areas of combination chemical genetics, toxicogenomics, drug chemical genomics and proteomics, and orthogonal chemical genetics. It describes the development and application of novel analytical methods used in lipodomics, such as steroidomics. The book also discusses biomarker discovery applications of microarray technologies using DNA, RNA, and protein and glycan arrays. Chapters cover further applications of biomolecular biomarkers for disease diagnosis, in small molecule drug R&D, and during therapeutic use of medicines. These include prognostic, disease specific, response (surrogate), and toxicity biomarkers. In addition, the text explores the principles of contemporary systems biology and genomics in experimental medicine-a new paradigm that demonstrates a network-oriented view and advanced statistical and informatics data management, opening the way toward personalized medicine. Finally, various in silico chemogenomics approaches are addressed for predicting binding of drug candidates to undesirable targets, which would help in designing better clinical candidates with fewer side effects. This new edition benefits a broad range of readers from industrial and academic researchers in drug discovery, medicinal chemistry, and molecular and cell biology to physicians in clinical diagnostics and students in related fields of study.

This title includes a number of Open Access chapters. Climate change will severely impact the world's food supply unless steps are taken to increase crop resilience. Otherwise, the negative effects on both the yield and the quality of crop plants are predicted to be immense. Plant genomics is a potentially powerful defense against this looming threat. This compendium volume offers a global perspective on the topic, with contributions from 42 eminent researchers from 12 nations around the world. The editor is a respected and published scientist in the bioinformatics field, who has chosen articles in the following topics: An overview of the genetic challenges presented by climate change A genomic toolkit for crop-related research Specific methods of improvement for specific crop by means of genomic applications The hand-picked up-to-date research makes this volume an excellent reference not only for university-level academics, but also for policymakers and stakeholders who must tackle the challenge of the world's food security.

Kruppel-like factors (KLFs) are attracting great attention across a wide spectrum of biological sciences and medicine because of their remarkable biological potency and the diversity of roles they play in the physiological and pathological changes of cells and tissues. This book is a comprehensive compendium of the latest research on the molecular mechanisms of KLFs, describing their roles in transcriptional regulation, cellular differentiation and development, the pathogenesis of the liver and cardiovascular systems and cancer, and generation of ES cells and iPS cells. As the only concise treatise written to date by leading experts in the field, it serves as an authoritative review of this family of molecules and is an essential reference for all who are interested in KLFs. The book also explores the potential of KLFs as targets for novel therapeutics and diagnostics, and will be invaluable in those fields.

What should individuals and society do when genetic screening becomes widely available and with its impact on current and future generations still uncertain? How can our education systems around the world respond to these developments? Reproductive and genetic technologies (RGTs) are increasingly controversial and political. We are entering an era where we can design future humans, firstly, by genetic screening of "undesirable" traits or indeed embryos, but perhaps later by more radical genetic engineering. This has a profound effect on what we see as normal, acceptable and responsible. This book argues that these urgent and biopolitical issues should be central to how biology is taught as a subject. Debate about life itself has always been at the forefront of connected molecular, genetic and social/personal identity levels, and each of these levels requires processes of communication and debate, what Anthony Giddens called in passing life politics. In this book Padraig Murphy opens the term up, with examples from field research in schools, student responses to educational films exploring the future of RGTs, and science studies of strategic biotechnology and the lab practices of genetic screening. Life political debate is thoroughly examined and is identified as a way of connecting mainstream education of biology with future generations. Biotechnology, Education and Life Politics will appeal to post-graduates and academics involved with science education, science communication, communication studies and the sociology of education.

Human genetics has blossomed from an obscure biological science and explanation for rare disorders to a field that is profoundly altering health care for everyone. This thoroughly updated new edition of Human Genetics: The Basics provides a concise background of gene structure and function through the lens of real examples, from families living with inherited diseases to population-wide efforts in which millions of average people are learning about their genetic selves. The book raises compelling issues concerning: * The role of genes in maintaining health and explaining sickness * Genetic testing, gene therapy, and genome editing * The common ancestry of all humanity and how we are affecting our future. Written in an engaging, narrative manner, this concise introduction is an ideal starting point for anyone who wants to know more about genes, DNA, genomes, and the genetic ties that bind us all.

First published in 1961, this book explains the main trends and problems in modern biological thought, at that time. It was based on lectures presented at the University College of the West Indies, Jamaica, in 1960 to members from different faculties and is therefore an accessible guide for all to the subject.

Laboratory studies in hemostasis have traditionally focused on abn- malities of platelet function or the quantitative and qualitative disorders that affect the proteins involved in blood coagulation. However, over the last 10 years there has been an explosion in our understanding of the molecular bases that underlie many of the inherited and acquired disorders of hemostasis. Many of these disorders are now routinely diagnosed and assessed by methods that involve genotypic analysis. Indeed in the late 1990s the distinction between molecular methods for research and for routine diagnosis is becoming incre- ingly blurred. The techniques and approaches that are used in hemostasis are manifold and published in isolation in a variety of publications. The aim, therefore, of this volume Hemostasis and Thrombosis Protocols is to pull together, into a single volume, the variety of techniques that are frequently used in the field of hemostasis. We have targeted this volume at laboratories who wish to move into the field of molecular hemostasis or who may already have some expe- ence in this area but wish to develop new areas of research and diagnosis. The chapters are wide-ranging and hopefully provide a broad overview of the d- fering applications in which these standard techniques can be used. Though the articles may appear relatively specific, the techniques contained within them are applicable to the study of many different disorders and we hope that they provide a series of ideas and concepts well-suited to problem solving.

Taking us to the cutting edge of the new frontier of medicine, a visionary biotechnologist and a pathbreaking researcher show how we can optimize our health in ways that were previously unimaginable. We are on the cusp of a major transformation in healthcare—yet few people know it. At top hospitals and a few innovative health-tech startups, scientists are working closely with patients to dramatically extend their “healthspan”—the number of healthy years before disease sets in. In The Age of Scientific Wellness, two visionary leaders of this revolution in health take us on a thrilling journey to this new frontier of medicine. Today, most doctors wait for clinical symptoms to appear before they act, and the ten most commonly prescribed medications confer little or no benefit to most people taking them. Leroy Hood and Nathan Price argue that we must move beyond this reactive, hit-or-miss approach to usher in real precision health—a form of highly personalized care they call “scientific wellness.” Using information gleaned from our blood and genes and tapping into the data revolution made possible by AI, doctors can catch the onset of disease years before symptoms arise, revolutionizing prevention. Current applications have shown startling results: diabetes reversed, cancers eliminated, Alzheimer’s avoided, autoimmune conditions kept at bay. This is not a future fantasy: it is already happening, but only for a few patients and at high cost. It’s time to make this gold standard of care more widely available. Inspiring in its possibilities, radical in its conclusions, The Age of Scientific Wellness shares actionable insights to help you chart a course to a longer, healthier, and more fulfilling life.

Computer solutions to many difficult problems in science and engineering require the use of automatic search methods that consider a large number of possible solutions to the given problems. This book describes recent advances in the theory and practice of one such search method, called Genetic Algorithms. Genetic algorithms are evolutionary search techniques based on principles derived from natural population genetics, and are currently being applied to a variety of difficult problems in science, engineering, and artificial intelligence.

First published in 1942 (second impression 1944), this book forms a debate about the endeavour to find an intellectual basis for ethics in science.

First published in 1960, this book discusses the ethical implications of the view of man's nature and his place in the biological world. C. H. Waddington highlights issues of the time, such as social upheavals related to social mobility, and the changing nature of philosophical thinking in relation to the nature of good. The author argues that man differs from all other animals in his ability for social teaching and learning and that this provides him with a second method of evolutionary advance, in addition to biology. He advances this through the idea that man has the capacity to entertain ethical ideas, which is an essential and necessary feature of this new mode of evolution. From here he draws the conclusion that a consideration of the broad trends of evolution provides a framework within which we can rationally discuss the relative merits of the various systems of ethical belief current in the world. In presenting his argument, Waddington draws on research in biology, psychology, the social sciences, and philosophy. He concludes with a short consideration of some of the most important ethical problems facing mankind at the time of the book's publication.

As a scientist, David Linden had devoted his career to understanding the brain processes and behaviors that are common to us all. That is, until a few years ago, when he found himself on OKCupid. Looking through that vast catalog of human difference, he got to thinking, where does it all come from? Why does one person have perfect pitch, a taste for hoppy beer, and an aversion to bathroom selfies? That is, what makes you, you, and me, me? In Unique, David Linden tells a riveting and accessible story of human individuality. Exploring topics that touch all of our lives-among them sexuality, gender identity, food preferences, biological rhythms, mood, personality, memory, and intelligence-Linden shows that human individuality is not simply a matter of nature versus nurture. Rather, it is a product of the complex, and often counterintuitive, interplay between our genetic blueprints and our experiences. Experience isn't just the how your parents reared you, but the diseases you have had, the foods you have eaten, the bacteria that reside in your body, the weather during your early development, and the technology you've been exposed to. Drawing all those factors together, Linden argues that human individuality is key to how we live as individuals and groups and explores how questions of individuality are informing social discussions of morality, public policy, religion, healthcare, education, and law. Like Carl Zimmer's She Has Her Mother's Laugh and Robert Sapolsky's Behave, Unique unveils a new vista on the intricacies of human existence. But, for all its brilliance and insight, this is no weighty academic tome. Told with Linden's unusual combination of authority and openness, seriousness of purpose and a great sense of humor, Unique sets a new standard for what popular science can be.

This second edition provides new and updated chapters from expert researchers in the field detailing methods used to study the multi-facet deep sequencing data field. Chapters guide readers through techniques for processing RNA-seq data, microbiome analysis, deep learning methodologies, and various approaches for the identification of sequence variants. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Deep Sequencing Data Analysis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

The analysis and interpretation of data is fundamental to the subject of genetics and forms a compulsory part of the undergraduate genetics curriculum. Indeed, the key skills that a genetics student requires are an ability to design and understand experimental strategies and to use problem-solving skills to interpret experimental results and data. Genetics? No Problem! provides students with a graded set of problems that aim to enthuse, challenge and entertain the reader. The book is divided into three sections introductory; intermediate and advanced each with 10 problems. For first level students there will be short genetics problems embedded in a wide range of scenarios, such as murder mysteries. As the book progresses, the stories will get longer and the science will get progressively more complex to challenge final year students and enable the reader to identify genetic disease in obscure organisms as well as designing and testing treatments and cures. Genetics? No Problem!: * Takes a unique, innovative approach that provides students with a set of graded problems designed to develop both their skills, and their ability to tackle problems with confidence * Includes problems embedded in a narrative, written in an interesting, informative and entertaining style by an Author with a proven track record in teaching, research and communication * Is well illustrated in full colour throughout. The book will prove invaluable to all students of genetics across a range of disciplines needing to get to grips with the analysis and interpretation of data that is fundamental to the subject.

RNA Modification provides a useful examination of the science and its role in biological regulation, the current frontier of life science research, and includes various RNA modications and their role in gene expression. It represents the most up-to-date knowledge and protocols available today.

First published in 1939 (second impression in 1950), this book provides an account of the changes in, and main principles of, genetics at that time. These are illustrated by references to the most authoritative and then recent investigations. Special attention is paid to the way in which genetics overlaps with other fields of inquiry, since it is often in these border-line subjects that the most important advances are to be expected. The book is particularly arranged to suit the convenience of students whose previous knowledge of genetics is small, and contains annotated bibliographies of suggestions for further reading.

The existence of genes for RNA molecules not coding for proteins (ncRNAs) has been recognized since the 1950's, but until recently, aside from the critically important ribosomal and transfer RNA genes, most focus has been on protein coding genes. However, a long series of striking discoveries, from RNA's ability to carry out catalytic function, to discovery of riboswitches, microRNAs and other ribo-regulators performing critical tasks in essentially all living organisms, has created a burgeoning interest in this primordial component of the biosphere. However, the structural characteristics and evolutionary constraints on RNA molecules are very different from those of proteins, necessitating development of a completely new suite of informatic tools to address these challenges. In RNA Sequence, Structure, Function: Computational and Bioinformatic Methods, expert researchers in the field describe a substantial and relevant fraction of these methodologies from both practical and computational/algorithmic perspectives. Focusing on both of these directions addresses both the biologist interested in knowing more about RNA bioinformatics as well as the bioinformaticist interested in more detailed aspects of the algorithms. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results. Thorough and intuitive, RNA Sequence, Structure, Function: Computational and Bioinformatic Methods aids scientists in continuing to study key methods and principles of RNA bioinformatics.

First published in 1935 (this edition in 1946), this short account of the science of embryology was the first book in English to provide a simple outline of the whole of this important subject. The study of development is perhaps the best method of approach to the most fundamental of all biological problems, the problem of how all the diverse activities are integrated so as to make up a complete individual organism. The book gives a short sketch of the general pattern on which all animals are built, but devotes more attention to the factors which cause the development of the elements in the pattern, and which then bring them into correct relations with one another. This volume is simply written in order to enable the general reader to understand the revolutionary advances made in the subject at that time.

Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.