Cytogenetic Laboratory Management: Chromosomal, FISH and Microarray-Based Best Practices and Procedures is a practical guide that describes how to develop and implement best practice processes and procedures in the genetic laboratory setting. The text first describes good laboratory practices, including quality management, design control of tests and FDA guidelines for laboratory developed tests, and pre-clinical validation study designs. The second focus of the book describes best practices for staffing and training, including cost of testing, staffing requirements, process improvement using Six Sigma techniques, training and competency guidelines and complete training programs for cytogenetic and molecular genetic technologists. The third part of the text provides step-wise standard operating procedures for chromosomal, FISH and microarray-based tests, including pre-analytic, analytic and post-analytic steps in testing, and divided into categories by specimen type, and test-type. All three sections of the book include example worksheets, procedures, and other illustrative examples that can be downloaded from the Wiley website to be used directly without having to develop prototypes in your laboratory. Providing both a wealth of information on laboratory management and molecular and cytogenetic testing, Cytogenetic Laboratory Management will be an essential tool for laboratorians world-wide in the field of laboratory testing and genetics testing in particular. This book gives the essentials of: * Developing and implementing good quality management programs in laboratories * Understanding design control of tests and pre-clinical validations studies and reports * FDA guidelines for laboratory developed tests * Use of reagents, instruments and equipment * Cost of testing assessment and process improvement using Six Sigma methodology * Staffing training and competency objectives * Complete training programs for molecular and cytogenetic technologists * Standard operating procedures for all components of chromosomal analysis, FISH and microarray testing of different specimen types This volume is a companion to Cytogenetic Abnormalities: Chromosomal, FISH and Microarray-Based Clinical Reporting. The combined volumes give an expansive approach to performing, reporting and interpreting cytogenetic laboratory testing and the necessary management practices, staff and testing requirements.

Kari Byron-former host of the wildly popular, iconic cult classic MythBusters-shows how to crash test your way through life, no lab coat required. Kari Byron's story hasn't been a straight line. She started out as a broke artist living in San Francisco, writing poems on a crowded bus on the way to one of her three jobs. Many curve balls, unexpected twists, and yes, literal and figurative explosions later, and she's one of the world's most respected women in science entertainment, blowing stuff up on national television and getting paid for it! In Crash Test Girl, Kari reveals her fascinating life story on the set of MythBusters and beyond. With her signature gusto and roll-up-your-sleeves enthusiasm, she invites readers behind the duct tape and the dynamite, to the unlikely friendships and low-budget sets that turned a crazy idea into a famously inventive show with a rabid fanbase. The truth is, Mythbusters was never meant to be a science show. But attaching a rocket to a car, riding a motorcycle on water, or lighting 500 pounds of coffee creamer on fire requires a decent understanding of chemistry, physics, and engineering. Thus, the cast and crew brought in the scientific method to work through each problem: Question. Hypothesize. Experiment. Analyze. Conclude. And as Kari came to learn in her own life, not only is the scientific method the best approach for busting myths, it's also the perfect tool for solving everyday issues, including: Career * Love * Creativity * Setbacks * Money * Sexuality * Depression * Bravery Crash Test Girl reminds us that science is for everyone, as long as you're willing to strap in, put on your safety goggles, hit a few walls, and learn from the results. Using a combination of methodical experimentation and unconventional creativity, you'll come to the most important conclusion of all: In life, sometimes you crash and burn, but you can always crash and learn.

Special Details: Equipment Testing Procedure. Softcover Member and other discounts do not apply to this title.

The first book on the topic, with each chapter written by pioneers in the field, this essential resource details the fundamental theory, applications, and future developments of liquid cell electron microscopy. This book describes the techniques that have been developed to image liquids in both transmission and scanning electron microscopes, including general strategies for examining liquids, closed and open cell electron microscopy, experimental design, resolution, and electron beam effects. A wealth of practical guidance is provided, and applications are described in areas such as electrochemistry, corrosion and batteries, nanocrystal growth, biomineralization, biomaterials and biological processes, beam-induced processing, and fluid physics. The book also looks ahead to the future development of the technique, discussing technical advances that will enable higher resolution, analytical microscopy, and even holography of liquid samples. This is essential reading for researchers and practitioners alike.

This textbook helps you to prepare for your next exams and practical courses by combining theory with virtual lab simulations. The "Labster Virtual Lab Experiments" series gives you a unique opportunity to apply your newly acquired knowledge in a learning game that simulates exciting laboratory experiments. Try out different techniques and work with machines that you otherwise wouldn't have access to.In this book, you'll learn the fundamental concepts of basic biochemistry focusing on: Ionic and Covalent Bonds Introduction to Biological Macromolecules Carbohydrates Enzyme Kinetics In each chapter, you'll be introduced to one virtual lab simulation and a true-to-life challenge. Following a theory section, you'll be able to play the relevant simulation that includes quiz questions to reinforce your understanding of the covered topics. 3D animations will show you molecular processes not otherwise visible to the human eye. If you have purchased a printed copy of this book, you get free access to five simulations for the duration of six months. If you're using the e-book version, you can sign up and buy access to the simulations at www.labster.com/springer. If you like this book, try out other topics in this series, including "Basic Biology", "Basic Genetics", and "Genetics of Human Diseases". Please note that the simulations in the book are not virtual reality (VR) but 2D virtual experiments.

Die rasante Entwicklung der Informationstechnologien forciert den Wandel im Gesundheitswesen und damit die neuen Rollenverstandnisse von Patient, Arzt, Gesundheitsdienstleister und Life-Science-Industrie. Die sich anbahnenden Chancen und Risiken im Gesundheitswesen sind so vielseitig, wie das Verstandnis des Begriffes e-Health. In kompakter und verstandlicher Form werden sowohl fundierte Basiskenntnisse vermittelt als auch Praxis-Perspektiven mit Fallbeispielen fur e-Health aufgezeigt.

The 'post genomics' era has seen a surge in demand for the techniques of cell biology, to aid in interpreting the function and location of the cell's myriad proteins and macromolecules. This new edition of Plant Cell Biology provides a grounding in established procedures before guiding the reader through the field's most current techniques. It provides advanced undergraduates, postgraduates and research staff in the plant sciences with a uniquely comprehensive guide to this rapidly expanding discipline.

This Primer provides an authoritative and easy to read overview of computers and their use in chemistry. It presents the essential basic ideas required to understand and exploit computers as encountered by chemistry students in their studies and in the laboratory at all stages up to and including research level. It gives its readers an insight into the workings of computers and so helps them to use the facilities more effectively.

Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy. Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocols for well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of the Interferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.

It has been recognised for many years that power ultrasound has great potential for uses in a wide variety of processes in the chemical and allied industries. Reported applications include cleaning, sterilisation, flotation, drying, degassing, defoaming, soldering, plastic welding, drilling, filtration, homogenisation, emulsification, dissolution, deaggregation of powder, biological cell disruption, extraction, crystallisation and, more recently, as a stimulus for chemical reactions. With the increasing use of power ultrasound in chemistry comes the need for specialist text books covering this emerging discipline. This book is suitable for chemists and chemical engineers at all levels who wish to gain a rapid insight into what is becoming a standard method in the chemical laboratory. It is designed to answer the following questions: What is sonochemistry? Why is it important? What systems are affected by sonochemistry? What are the major applications of this technique? What types of ultrasonic equipment are available? How do the different types of equipment compare? How is the equipment best configured for chemical applications? What parameters can be changed to optimise sonochemical results? What equipment is available for the scale-up of sonochemistry?

This is the bench and field scientist's guide to well-established, reliable techniques for use in microbiology and microbial ecology. It provides a good starting place for those who are beginning to investigate aspects of the microbial community, and a refresher for more experienced researchers. Chapters on bacteria with interesting metabolic traits are augmented with chapters on molecular techniques, lipis analysis, and appropriate sampling techniques. A special section includes valuable information on biofilm development, bioremediation, modeling of biological systems, and the study of phylogenetics. Unlike other texts, which present theory in microbial ecology, this one contains the applications that can be used throughout one's research.

This book makes life easier for those developing a computerized experiment. It is full of information to enable scientists and engineers to find their way through the many computing and electronics topics associated with computerized measurement. It draws together the knowledge of several fields in an easy to understand manner, and comes complete with a disk containing all the many useful examples of software and other information listed in the text.

Die nichtinvasive Erfassung des arteriellen Pulswellensignals erlaubt Pulsmessung, Pulsoximetrie bis hin zu Gefassrisikobewertung direkt am Unterarm. Robert Couronne beschreibt zwei Modellsimulationen der Photonenausbreitung im Unterarmgewebe. Anhand gemessener Intensitaten verifiziert der Autor die erweiterte Lambert-Beer-Beziehung eines planaren Schichtenmodells und untersucht mithilfe eines Zylindermodells die Intensitatsverteilung entlang des Unterarmumfangs, wobei bevorzugte Ableitregionen identifiziert werden. Zum experimentellen Nachweis stellt der Autor den Prototyp eines optisch-transmissiven Mehrkanalsensorsystems vor und zeigt anhand von Probandenmessungen die technische Signalgewinnung und praktische Anwendbarkeit. Mit einem Klassifikationsverfahren kann die automatische Signalidentifikation sowie eine Qualitatsbewertung im Realzeitbetrieb erreicht werden. Die vorgestellten Verfahren sind dabei auf gangigen Mikrocontroller-Plattformen lauffahig."

An ideal introduction to this fast growing area, this Primer describes the many computational methods currently used by practising chemists. The authors describe the various techniques available, and how they can be applied to single molecules, to assemblies of molecules, and to molecules undergoing reaction. An introductory chapter outlines hardware and software options, as well as investigating some applications and developments. Subsequent chapters cover quantum mechanics, molecular mechanics, statistical mechanics, the modelling of biomolecules, and drug design.

This essential laboratory handbook dispels the mystery surrounding organocopper compounds and offers expert guidance on how to overcome the many problems associated with their use. Authored by leading researchers, the book features clear, practical descriptions of key procedures used in the preparation and application of these highly specific reagents. In addition, the book contains a succinct historical overview of the field's development. Beginning and established researchers alike will find Organocopper Reagents: A Practical Approach an invaluable source of information on an exciting, fast-growing field.

This book provides a comprehensive account of the theory of image formation in a confocal fluorescence microscope as well as a practical guideline to the operation of the instrument, its limitations, and the interpretation of confocal microscopy data. The appendices provide a quick reference to optical theory, microscopy-related formulas and definitions, and Fourier theory.

Plant cell culture techniques are being increasingly used in basic research and for plant exploitation in industry (genetic engineering, micropropagation). In this book, internationally recognized experts describe the techniques in use in their laboratories for isolating and manipulating cell cultures, and their central importance in plant biotechnology.

Modern chemical experiments rely heavily on electronic instrumentation; some techniques in common use owe their existence to electronics. Yet undegraduates reading chemistry often have only the most rudimentary understanding of the way in which electronics and allied disciplines have provided powerful tools that enable creative experimentation to be performed. This book aims to provide an understanding of some of the basic techniques that are harnessed for the benefit of chemists.

The use of stable isotopes in nutritional studies is now widespread, and the technique is becoming increasingly popular. Practical applications are numerous and include: calcium and iron absorption studies, studies looking at the impacts of diet, physical activity, aging, and medical therapy and supplementation on nutrient metabolism, the measurement of energy cost of pregnancy, studies on the causes of growth faltering in infants, and investigations into childhood and adult obesity. This book is designed as a laboratory handbook of methods used to perform stable isotope studies in humans. It covers basic principles, dosage information, sample preparation procedures, analytical instrumentation, and necessary mathematical methods and provides the fundamentals to enable researchers to evaluate and establish stable isotope methods in their own laboratories.

Written by a pioneer in the field, this text provides a complete introduction to X-ray microscopy, providing all of the technical background required to use, understand and even develop X-ray microscopes. Starting from the basics of X-ray physics and focusing optics, it goes on to cover imaging theory, tomography, chemical and elemental analysis, lensless imaging, computational methods, instrumentation, radiation damage, and cryomicroscopy, and includes a survey of recent scientific applications. Designed as a 'one-stop' text, it provides a unified notation, and shows how computational methods in different areas are linked with one another. Including numerous derivations, and illustrated with dozens of examples throughout, this is an essential text for academics and practitioners across engineering, the physical sciences and the life sciences who use X-ray microscopy to analyze their specimens, as well as those taking courses in X-ray microscopy.