This is an extensively illustrated laboratory manual of transmission electron microscopy techniques for the technician, graduate student, or researcher. Chapters begin with a general discussion, move on to the chemicals and equipment required for the method being described and conclude with a step-by-step presentation of the method and instructions for the preparation of solutions. Notes at the end of each chapter warn of possible pitfalls and outline 'tricks of the trade'. The methods and techniques outlined have been tested for over ten years in clinical and research laboratory situations, and are entirely reliable. Practical Electron Microscopy covers fixation, dehydration and embedding, semi-thin and thin sectioning, the electron microscope, and photography. For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added, one on immuno electron microscopy using colloidal gold, and one dealing with such special techniques as retrieving specimens from paraffin and handling nasal brushings and blood samples. This manual will be an invaluable guide to anyone using electron microscopy on human and animal tissue and wishing to develop a routine that guarantees good and reproducible results.

The compound optical microscope, in its various modern forms, is probably the most familiar of all laboratory instruments and the electron microscope, once an exotic rarity, has now become a standard tool in biological and materials research. Both instruments are often used effectively with little knowledge of the relevant theory, or even of how a particular type of microscope functions. Eventually however, proper use, interpretation of images and choices of specific applications demand an understanding of fundamental principles. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, accounts for ultimate limits on the size of observable details (resolving power and resolution) and finally provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. The book will appeal to graduate students and researchers in the biomedical sciences, and it will be helpful to students taking a course involving the principles of microscopy.

Die Proceedings der 35. Jahrestagung der Gesellschaft fur Medizinische Dokumentation, Informatik und Statistik geben einen UEberblick uber die derzeit wichtigen Forschungsschwerpunkte der Epidemiologie. Dabei konnten zum ersten Mal auch Beitrage aus der ehemaligen DDR in groesserer Zahl eingebracht werden. Schwerpunkte des Proceedingsbandes bilden die Epidemiologie kardiovaskularer Erkrankungen, die Umweltepidemiologie, die Epidemiologie von Berufskrankheiten und das epidemiologische Monitoring von Krebserkrankungen und Infektionskrankheiten. Die Qualitat verfugbarer Datenquellen wird kritisch diskutiert und die Bedeutung der Epidemiologie fur eine Verbesserung der Gesundheitsberichterstattung in den alten und in den neuen Bundeslandern klargelegt. Daruber hinaus werden methodische Probleme und unterstutzende Techniken der Medizinischen Informatik angesprochen.

Der Proceedingsband enth{lt ausgew{hlte ausgew{hlte refe- rierte Aufs{tze sowohl zu innovativen Entwicklungen in den Disziplinen Biometrie und Medizinische Informatik als auch }ber den Einsatz bekannter, bew{hrter Konzepte aus diesen Fachbereichen in der Medizin. Behandelt werden spezielle Fragestellungen zu den Themen Epidemiologie, methodische Therapieforschung, Nachweis therapeutischer Oquivalenz, Dia- gnostik und Monitoring von Krankheitsprozessen, Modellierung biologischer Mechanismen, biometrische Methoden, medizini- sche Dokumentation, L-sungsans{tze f}r Informationssysteme, spezielle Hard- und Softwarel-sungen, Expertensysteme, Bild- kommunikation und Archivierung, Bilderzeugung, wissensba- sierte Bildinterpretation und 3-d Darstellungsverfahren.

This textbook provides a comprehensive introduction to the theory and practice of validated numerics, an emerging new field that combines the strengths of scientific computing and pure mathematics. In numerous fields ranging from pharmaceutics and engineering to weather prediction and robotics, fast and precise computations are essential. Based on the theory of set-valued analysis, a new suite of numerical methods is developed, producing efficient and reliable solvers for numerous problems in nonlinear analysis. Validated numerics yields rigorous computations that can find all possible solutions to a problem while taking into account all possible sources of error--fast, and with guaranteed accuracy.

"Validated Numerics" offers a self-contained primer on the subject, guiding readers from the basics to more advanced concepts and techniques. This book is an essential resource for those entering this fast-developing field, and it is also the ideal textbook for graduate students and advanced undergraduates needing an accessible introduction to the subject. "Validated Numerics" features many examples, exercises, and computer labs using MATLAB/C++, as well as detailed appendixes and an extensive bibliography for further reading.Provides a comprehensive, self-contained introduction to validated numerics Requires no advanced mathematics or programming skills Features many examples, exercises, and computer labs Includes code snippets that illustrate implementation Suitable as a textbook for graduate students and advanced undergraduates

This book traces the historical development of microscopy instruments from their invention to the current state of the art. New concepts and engineering solutions are presented for modern light microscopes, with a focus on the practical construction of optical systems. Real design parameters of dioptric objectives and other systems are provided to supply readers with basic information for independent designs. Full-color photomicrographs of real objects illustrate the quality of aberration correction that is required from optics.

Microscope Image Processing, Second Edition, introduces the basic fundamentals of image formation in microscopy including the importance of image digitization and display, which are key to quality visualization. Image processing and analysis are discussed in detail to provide readers with the tools necessary to improve the visual quality of images, and to extract quantitative information. Basic techniques such as image enhancement, filtering, segmentation, object measurement, and pattern recognition cover concepts integral to image processing. In addition, chapters on specific modern microscopy techniques such as fluorescence imaging, multispectral imaging, three-dimensional imaging and time-lapse imaging, introduce these key areas with emphasis on the differences among the various techniques. The new edition discusses recent developments in microscopy such as light sheet microscopy, digital microscopy, whole slide imaging, and the use of deep learning techniques for image segmentation and analysis with big data image informatics and management. Microscope Image Processing, Second Edition, is suitable for engineers, scientists, clinicians, post-graduate fellows and graduate students working in bioengineering, biomedical engineering, biology, medicine, chemistry, pharmacology and related fields, who use microscopes in their work and would like to understand the methodologies and capabilities of the latest digital image processing techniques or desire to develop their own image processing algorithms and software for specific applications.

Gottingen ist mit den Aufgaben und Zielen der Deutschen Gesellschaft fUr Medizinische Dokumentation, Informatik und Statistik (GMDS) nicht nur durch die yom 3. - 5. Oktober 1977 stattgefundene 22. Jahrestagung verbunden. Schon durch den Mathematiker Felix B ern s t e i n, dem anlaBlich seines 100. Geburtstages eine Diplomarbeit aus dem Mathe- matisch-Statistischen Institut der Universitat Gotting n gewidmet worden ist, wurden bereits vor dem ersten Weltkrieg und in den darauf- folgenden Jahren grundlegende mathematisch-statistische Arbeiten auf dem Gebiet des Versicherungswesens und der Humangenetik durchgefUhrt. Nach dem zweiten Weltkrieg hat H 0 s e man n in Gottingen die Ver- fahren der maschinellen Lochkartendokumentation zur Datensammlung als Grundlage statistischer Verfahren fUr die Universitats-Frauenklinik eingesetzt. Ein Teil seiner Entwicklungen, besonders auf dem Gebiet der Erfassung und Standardisierung der gynakologischen Karzinome werden heute noch verwendet. Ende 1952 und 1954 war Gottingen Tagungsort der Untergruppe Medizin der Deutschen Gesellschaft fUr Dokumentation (DGD) und nach GrUndung des "ArbeitsausschuB Medizin" in der DGD, dem direkten Vorlaufer unserer Gesellschaft, wurde die e r s t e Jahrestagung in Gottingen mit dem Thema: "Lochkarten und Randlochkarten im klinischen Gebrauch" durchgefUhrt.