The Welfare of Animals used in Research: Practice and Ethics gives a complete and balanced overview of the issues surrounding the use of animals in scientific research. The focus of the book is on the animal welfare implications and ethics of animals in research. It covers the topics with sufficient depth to show a real understanding of varied and complex subjects, but conveys the information in a beautifully reader-friendly manner. Key features: * Provides those who are not working in the field with a reasonable understanding as to why and how animals are used in research. * Gives an introduction to the ethical issues involved in using animals, and explains how these are addressed in practice. * Details the advances in animal welfare and the use and development of the 3Rs principles, and how these have become fundamental to the everyday use and regulation of animals used in research. * The focus is on principles making it suitable for an international audience. This book is a useful introduction to the issues involved in laboratory animal welfare for those who intend to work in research involving animals. It is also useful to prospective animal care staff and animal welfare scientists, and to those involved in ethical review. It will help inform debate amongst those who are not involved in experimentation but who are interested in the issues. Published as a part of the prestigious Wiley-Blackwell UFAW Animal Welfare series. UFAW, founded 1926, is an internationally recognised, independent, scientific and educational animal welfare charity. For full details of all titles available in the series, please visit the

Structural genomics is the systematic determination of 3-D structures of proteins representative of the range of protein structure and function found in nature. The goal is to build a body of structural information that will predict the structure and potential function for almost any protein from knowledge of its coding sequence. This is essential information for understanding the functioning of the human proteome, the ensemble of tens of thousands of proteins specified by the human genome.
While most structural biologists pursue structures of individual proteins or protein groups, specialists in structural genomics pursue structures of proteins on a genome wide scale. This implies large-scale cloning, expression and purification. One main advantage of this approach is economy of scale.
Key Features
*Examines the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography and NMR spectroscopy
* Looks at structural genomics as a foundation of drug discovery as discovering new medicines is becoming more challenging and the pharmaceutical industry is looking to new technologies to help in this mission

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

* Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide

This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

* Includestime-tested coremethods and new innovationsapplicable to any researcher * Methods included are useful to both establishedresearchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide"

This fully updated, self-contained textbook covering modern optical microscopy equips students with a solid understanding of the theory underlying a range of advanced techniques. Two new chapters cover pump-probe techniques, and imaging in scattering media, and additional material throughout covers light-sheet microscopy, image scanning microscopy, and much more. An array of practical techniques are discussed, from classical phase contrast and confocal microscopy, to holographic, structured illumination, multi-photon, and coherent Raman microscopy, and optical coherence tomography. Fundamental topics are also covered, including Fourier optics, partial coherence, 3D imaging theory, statistical optics, and the physics of scattering and fluorescence. With a wealth of end-of-chapter problems, and a solutions manual for instructors available online, this is an invaluable book for electrical engineering, biomedical engineering, and physics students taking graduate courses on optical microscopy, as well as advanced undergraduates, professionals, and researchers looking for an accessible introduction to the field.

This book explicates the optical controls of antiferromagnetic spins by intense terahertz (THz) electromagnetic waves. The book comprises two key components: (1) the experimental demonstration of the enhancement of a THz magnetic field using a split-ring resonator (SRR) and (2) the control of the direction of magnetization by using the enhanced THz magnetic field to break the symmetry of optically-induced phase transition. These make up the first step leading to future spintronics devices. In the beginning of the book, the author reviews the basics of the ultrafast laser and nonlinear optical techniques as well as the previously achieved experiments to control spin dynamics by THz magnetic fields. In this context, a new experimental protocol is described, in which electron spins in a ferromagnetic material are redirected at the unprecedented level in cooperation with the enhanced THz magnetic field. Subsequently, the author demonstrates that the THz magnetic field is significantly amplified as a nearfield around the SRR structured metamaterial, which is implemented by measuring spin precession in a solid. At the end, the author presents the key experiment in which the amplified THz magnetic nearfield is applied to the weak ferromagnet ErFeO3 along with the femtosecond near-infrared pulse, demonstrating the successful control of symmetry breaking of the spin system due to coherent control of the optically-induced spin reorientation phase transition pathways. The comprehensive introductory review in this book allows readers to overview state-of-the-art terahertz spectroscopic techniques. In addition, the skillful description of the experiments is highly informative for readers in ultrafast magnonics, ultrafast optics, terahertz technology and plasmonic science.

In Laser Physics the interaction of radiation and matter, and the principles of laser operation are treated at a level suitable for fourth-year undergraduate courses or introductory graduate courses in physics, chemistry or engineering. The factors which determine efficiency, wavelength coverage, output power, and beam quality of the different classes of laser are treated both in terms of fundamental theory and practical construction aspects. Details of established types of solid-state, semiconductor, and gas lasers are examined together with the techniques that enable their output to be converted widely across the spectrum. The latest advances in high power fibre lasers, femtosecond lasers, and X-ray lasers are explained. The text is liberally illustrated with more than 300 diagrams. An extensive bibliography is provided, together with numerical problems in each chapter. Solutions are available via the web.
To request a copy of the Solutions Manual, visit: http: //global.oup.com/uk/academic/physics/admin/solutions

As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.

It documents many diverse uses for confocal microscopy in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in the initial publications Techniques are described in a context that allows comparisons to other related methodologies. Methodologies are laid out in a manner that stresses their general applicability and reports their potential limitations.

Scientific techniques developed in materials science offer invaluable information to archaeology, art history, and conservation. A rapidly growing number of innovative methods, as well as many established techniques, are constantly being improved and optimized for the analysis of cultural heritage materials. The result is that on the one hand more complex problems and questions can be confronted, but on the other hand the required level of technical competence is widening the existing cultural gap between scientists and end users, such as archaeologists, museum curators, art historians, and many managers of cultural heritage who have a purely humanistic background.
The book is intended as an entry-level introduction to the methods and rationales of scientific investigation of cultural heritage materials, with emphasis placed on the analytical strategies, modes of operation, and resulting information rather than on technicalities. The extensive and updated reference list should be a useful starting point for further reading. Students and researchers from the humanities approaching scientific investigations should find it useful, as well as scientists applying familiar techniques and methods to unfamiliar problems related to cultural heritage.

X-ray fluorescence spectroscopy, one of the most powerful and flexible techniques available for the analysis and characterization of materials today, has gone through major changes during the past decade.

Fully revised and expanded by 30%, X-Ray Fluorescence Spectrometry, Second Edition incorporates the latest industrial and scientific trends in all areas. It updates all previous material and adds new chapters on such topics as the history of X-ray fluorescence spectroscopy, the design of X-ray spectrometers, state-of-the-art applications, and X-ray spectra.

Ron Jenkins draws on his extensive experience in training and consulting industry professionals for this clear and concise treatment, covering first the basic aspects of X rays, then the methodology of X-ray fluorescence spectroscopy and available instrumentation. He offers a comparison between wavelength and energy dispersive spectrometers as well as step-by-step guidelines to X-ray spectrometric techniques for qualitative and quantitative analysis—from specimen preparation to real-world industrial application.

Favored by the American Chemical Society and the International Centre for Diffraction Data, X-Ray Fluorescence Spectrometry, Second Edition is an ideal introduction for newcomers to the field and an invaluable reference for experienced spectroscopists—in chemical analysis, geology, metallurgy, and materials science.

An up-to-date review of X-ray spectroscopic techniques. This proven guidebook for industry professionals is thoroughly updated and expanded to reflect advances in X-ray analysis over the last decade. X-Ray Fluorescence Spectrometry, Second Edition includes:

• The history of X-ray fluorescence spectrometry—new to this edition.
• A critical review of the most useful X-ray spectrometers.
• Techniques and procedures for quantitative and qualitative analysis.
• Modern applications and industrial trends.
• X-ray spectra—new to this edition.

Computational Methods in Physics, Chemistry and Biology offers an accessible introduction to key computational techniques used within science, including quantum mechanics, dynamics, evolutionary methods and molecular dynamics. Assuming only a limited background in computational methods, this book provides the reader with a series of comprehensive examples, problems and practical-based tasks from the basics through to more complex ideas and techniques.

Beginning with an introduction to a numerical solution of Schrödinger's Equation the text moves on to discuss perturbation theory, variational calculations, diffusion, dynamics, Monte Carlo simulations and genetic algorithms. Aimed at those new to the field, the book will enable the reader to develop and implement computational methods for the solutions of a range of problems in science.

Features:

• An accessible introduction covering a range of essential techniques applicable to key problems
• Numerous examples, problems and practical-based exercises.
• A supplementary website including solutions

Acoustic microscopy enables the elastic properties of materials to be imaged and measured with the resolution of a good microscope. By using frequencies in the microwave regime, it is possible to make the acoustic wavelength comparable with the wavelength of light, and hence to achieve a resolution comparable with an optical microscope. Solids can support both longitudinal and transverse acoustic waves. At surfaces a unique combination of the two known as Raleigh waves can propagate, and in many circumstances these dominate the contrast in acoustic microscopy. Following the invention of scanning probe microscopes, it is now possible to use an atomic force microscope to detect the acoustic vibration of a surface with resolution in the nanometre range, thus beating the diffraction limit by operating in the extreme near-field. This second edition of Acoustic Microscopy has a major new chapter on the technique and applications of acoustically excited probe microscopy.

There is an increasing need for analysts to understand and be able to quantify the performance of analytical instruments, in particular with respect to the following:
* specifying equipment for purchase
* estimating uncertainties in intrumental measurements
* quantifying and demonstrating performance quality
This text links together an understanding of performance characteristics with an appreciation of the limitations imposed by instrument design, leading to the interplay of the validation and qualification processes within quality assurance systems.
A unique framework of topics covers the major instrumental techniques of spectrophotometry, chromatography, capillary electrophoresis, and atomic emission spectroscopy. The use of over 200 questions and answers, together with cross-referencing, helps to develop a thorough understanding of the various concepts that underpin the different techniques.
This book will appeal to a broad range of professional chemists, technicians and students, weither with reference to specific analytical techniques, or within a general course of study in instrumental performance.
Analytical Techniques in the Sciences
This series of books provides coverage of all the major analytical techniques and their application in the most important areas of physical, life and materials sciences. Each text is presented in an open learning/distant learning style, in which the learning objectives are clearly identified. The reader's understanding of the material is constantly evaluated by the use of self-assessment and discussion questions.

"Fundamentals of Light Microscopy and Electronic Imaging, Second Edition" provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.

PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website:

www.wiley.com/go/murphy/lightmicroscopy

This book provides an introduction/overview of the various facets of laboratory automation. Laboratory automation is an active area gaining intense exposure with the increased emphasis on productivity. Laboratory automation comes in many forms ranging from robotics which can allow for automated sample preparation and subsequent analysis to flow injection analysis and other forms of automated systems. The final facet of many automated systems, LIMS, will also be adressed. This book will serve as a reference, a general introductory volume on laboratory automation for industrial and academic scientists, and also as a text for an introductory or advanced course on laboratory automation. The reader can focus on a particular technology and obtain sufficient information, or read the entire book for a comprehensive view of the field of lab automation.

This textbook is an excellent guide to microscopy for students and scientists, who use microscopy as one of their primary research and analysis tool in the laboratory. The book covers key microscopy principles and explains the various techniques such as epifluorescence microscopy, confocal/live cell imaging, SIM/light sheet microscopy, and many more. Easy-to-understand protocols provide helpful guidance for practical implementation in various commercially available imaging systems. The reader is introduced to histology and further be guided through advanced image acquisition, classification and analysis. The book is written by experienced imaging specialists from the UK, other EU countries, the US and Asia, and is based on advanced training courses for master students and PhD students. Readers are not expected to be familiar with imaging and microscopy technologies, but are introduced to the subject step by step. This textbook is indented for biomedical and medical students, as well as scientists and postdocs who want to acquire a thorough knowledge of microscopy, or gain a comprehensive overview of modern microscopy techniques used in various research laboratories and imaging facilities. Chapter 4 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

A bestseller for nearly 25 years, Analysis of Messy Data, Volume 1: Designed Experiments helps applied statisticians and researchers analyze the kinds of data sets encountered in the real world. Written by two long-time researchers and professors, this second edition has been fully updated to reflect the many developments that have occurred since the original publication. New to the Second Edition Several modern suggestions for multiple comparison procedures Additional examples of split-plot designs and repeated measures designs The use of SAS-GLM to analyze an effects model The use of SAS-MIXED to analyze data in random effects experiments, mixed model experiments, and repeated measures experiments The book explores various techniques for multiple comparison procedures, random effects models, mixed models, split-plot experiments, and repeated measures designs. The authors implement the techniques using several statistical software packages and emphasize the distinction between design structure and the structure of treatments. They introduce each topic with examples, follow up with a theoretical discussion, and conclude with a case study. Bringing a classic work up to date, this edition will continue to show readers how to effectively analyze real-world, nonstandard data sets.

Digital image processing, an integral part of microscopy, is increasingly important to the fields of medicine and scientific research. This book provides a unique one-stop reference on the theory, technique, and applications of this technology.
Written by leading experts in the field, this book presents a unique practical perspective of state-of-the-art microscope image processing and the development of specialized algorithms. It contains in-depth analysis of methods coupled with the results of specific real-world experiments. Microscope Image Processing covers image digitization and display, object measurement and classification, autofocusing, and structured illumination.
Key Features:
- Detailed descriptions of many leading-edge methods and algorithms
- In-depth analysis of the method and experimental results, taken from real-life examples
- Emphasis on computational and algorithmic aspects of microscope image processing
-Advanced material on geometric, morphological, and wavelet image processing, fluorescence, three-dimensional and time-lapse microscopy, microscope image enhancement, MultiSpectral imaging, and image data management
This book is of interest to all scientists, engineers, clinicians, post-graduate fellows, and graduate students working in the fields of biology, medicine, chemistry, pharmacology, and other related fields. Anyone who uses microscopes in their work and needs to understand the methodologies and capabilities of the latest digital image processing techniques will find this book invaluable.
* Presents a unique practical perspective of state-of-the-art microcope image processing and the development of specialized algorithms.
*Each chapter includes in-depth analysis of methods coupled with the results of specific real-world experiments.
* Co-edited by Kenneth R. Castleman, world-renowned pioneer in digital image processing and author of two seminal textbooks on the subject.

This companion volume to "Fundamental Polymer Science" (Gedde and Hedenqvist, 2019) offers detailed insights from leading practitioners into experimental methods, simulation and modelling, mechanical and transport properties, processing, and sustainability issues. Separate chapters are devoted to thermal analysis, microscopy, spectroscopy, scattering methods, and chromatography. Special problems and pitfalls related to the study of polymers are addressed. Careful editing for consistency and cross-referencing among the chapters, high-quality graphics, worked-out examples, and numerous references to the specialist literature make "Applied Polymer Science" an essential reference for advanced students and practicing chemists, physicists, and engineers who want to solve problems with the use of polymeric materials.

Succeed in biology with LABORATORY MANUAL FOR NON-MAJORS BIOLOGY! Through hands-on lab experience, this biology laboratory manual reinforces biology concepts to help you get a better grade. Exercises, pre-lab questions, and post-lab questions enhance your understanding and make lab assignments easy to complete and easy to comprehend.

What can you learn about your world in just a moment? Have you ever wondered why the sky is blue? Or whether dogs can read our facial expressions? Don Glass and experts in their fields answer these questions and many more. Written for readers of all ages with no background in science required, How the World Looks to a Bee is the perfect armchair companion for curious people who want to know more about the science of everyday life but have only a moment to spare. With intriguing everyday phenomena as a starting point, this entertaining collection uses short tutorials and quick and simple experiments to invite readers to test the science for themselves. These fascinating and topical science stories are sure to delight the curious child in all of us.

Recent advances in the imaging technique electron microscopy (EM) have improved the method, making it more reliable and rewarding, particularly in its description of three-dimensional detail. This book will help biologists from many disciplines understand modern EM and the value it might bring to their own work. The book's five sections deal with all major issues in EM of cells: specimen preparation, imaging in 3-D, imaging and understanding frozen-hydrated samples, labeling macromolecules, and analyzing EM data. Each chapter was written by scientists who are among the best in their field, and some chapters provide multiple points of view on the issues they discuss. Each section of the book is preceded by an introduction, which should help newcomers understand the subject. The book shows why many biologists believe that modern EM will forge the link between light microscopy of live cells and atomic resolution studies of isolated macromolecules, helping us toward the goal of an atomic resolution understanding of living systems.
* Updates the numerous technological innovations that have improved the capabilities of electron microscopy
* Provides timely coverage of the subject given the significant rise in the number of biologists using light microscopy to answer their questions and the natural limitations of this kind of imaging
* Chapters include a balance of "how to," "so what" and "where next," providing the reader with both practical information, which is necessary to use these methods, and a sense of where the field is going

A complete guide to one of the most revolutionary technologies in the history of imaging

Near-field microscopes combine the richness of optical analysis, the noninvasive character of light, and the wide variety of sample environments of conventional microscopes with the finer spatial resolution of alternative technologies. Near-Field Optics combines an introduction to near-field optical theory with a handbook and reference for the practice and application of near-field microscopy. Michael A. Paesler and Patrick J. Moyer provide the most comprehensive presentation available on the instrumentation and operation of near-field microscopes. Writing from the viewpoint of the scientist who wants to apply these revolutionary instruments in a laboratory setting, the authors:

• Explain the pertinent optical theory and provide a developmental history of near-field instruments
• Discuss imaging theory and its application in the near-field scanning optical microscope (NSOM)
• Explore the optical behavior of elements that provide the near-field/far-field connection in an NSOM
• Provide operational how-to's for NSOMs
• Examine the theory and operation of optical tunneling microscopes with special emphasis on the photon tunneling microscope (PTM)
• Enumerate contrast mechanisms available to the near-field microscopist
• Describe the application of near-field techniques in biology, materials science, surface chemistry, and information storage

Soaring Gothic cathedrals, violent crusades, the Black Death: these are the dramatic forces that shaped the medieval era. But the so-called Dark Ages also gave us the first universities, eyeglasses, and mechanical clocks. As medieval thinkers sought to understand the world around them, from the passing of the seasons to the stars in the sky, they came to develop a vibrant scientific culture. In The Light Ages, Cambridge science historian Seb Falk takes us on a tour of medieval science through the eyes of one fourteenth-century monk, John of Westwyk. Born in a rural manor, educated in England's grandest monastery, and then exiled to a clifftop priory, Westwyk was an intrepid crusader, inventor, and astrologer. From multiplying Roman numerals to navigating by the stars, curing disease, and telling time with an ancient astrolabe, we learn emerging science alongside Westwyk and travel with him through the length and breadth of England and beyond its shores. On our way, we encounter a remarkable cast of characters: the clock-building English abbot with leprosy, the French craftsman-turned-spy, and the Persian polymath who founded the world's most advanced observatory. The Light Ages offers a gripping story of the struggles and successes of an ordinary man in a precarious world and conjures a vivid picture of medieval life as we have never seen it before. An enlightening history that argues that these times weren't so dark after all, The Light Ages shows how medieval ideas continue to color how we see the world today.

The experiments in this manual are designed in a discovery format and the majority require only small quantities of reagents.