The evolution of observational instruments, simulation techniques, and computing power has given aquatic scientists a new understanding of biological and physical processes that span temporal and spatial scales. This has created a need for a single volume that addresses concepts of scale in a manner that builds bridges between experimentalists and theoreticians in aquatic ecology. Handbook of Scaling Methods in Aquatic Ecology: Measurement, Analysis, Simulation is the first comprehensive compilation of modern scaling methods used in marine and freshwater ecological research. Written by leading researchers, it presents a systematic approach to dealing with space and time in aquatic ecology. This is a compendium that analyzes themes related to the response or behavior of organisms to processes occurring over multiple spatial and temporal scales. This book covers: novel techniques for data collection, focusing on processes over a broad range of scales (from bacteria to ocean basins); newly-developed concepts and data analysis algorithms; and innovative computer models and simulations to mimic complex biological processes. The Handbook serves as a reference volume for investigators seeking insight into new experimental approaches and data analysis, as well as the sensor design required for optimal sampling. Many of the algorithms and models provided are directly applicable to your experimental data. This comprehensive treatment of scaling methods and applications can help foster a unified understanding of subject matter among the modeling, experimental, and field research communities.

Dealing with the volume, complexity, and diversity of data currently being generated by scientific experiments and simulations often causes scientists to waste productive time. Scientific Data Management: Challenges, Technology, and Deployment describes cutting-edge technologies and solutions for managing and analyzing vast amounts of data, helping scientists focus on their scientific goals. The book begins with coverage of efficient storage systems, discussing how to write and read large volumes of data without slowing the simulation, analysis, or visualization processes. It then focuses on the efficient data movement and management of storage spaces and explores emerging database systems for scientific data. The book also addresses how to best organize data for analysis purposes, how to effectively conduct searches over large datasets, how to successfully automate multistep scientific process workflows, and how to automatically collect metadata and lineage information. This book provides a comprehensive understanding of the latest techniques for managing data during scientific exploration processes, from data generation to data analysis. Enhanced by numerous detailed color images, it includes real-world examples of applications drawn from biology, ecology, geology, climatology, and more. Check out Dr. Shoshani discuss the book during an interview with International Science Grid This Week (iSGTW): http://www.isgtw.org/?pid=1002259

This book aims to provide examples of applications of atomic force microscopy (AFM) using biological samples, showing different methods for AFM sample preparation, data acquisition and processing, and avoiding technical problems. Divided into two sections, chapters guide readers through image artifacts, process and quantitatively analyze AFM images, lipid bilayers, image DNA-protein complexes, AFM cell topography, single-molecule force spectroscopy, single-molecule dynamic force spectroscopy, fluorescence methodologies, molecular recognition force spectroscopy, biomechanical characterization, AFM-based biosensor setup, and detail how to implement such an in vitro system, which can monitor cardiac electrophysiology, intracellular calcium dynamics, and single cell mechanics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Atomic Force Microscopy: Methods and Protocols is useful for researchers at different stages, from newcomers to experienced users, interested in new AFM applications.

This expansive and practical textbook contains organic chemistry experiments for teaching in the laboratory at the undergraduate level covering a range of functional group transformations and key organic reactions.The editorial team have collected contributions from around the world and standardized them for publication. Each experiment will explore a modern chemistry scenario, such as: sustainable chemistry; application in the pharmaceutical industry; catalysis and material sciences, to name a few. All the experiments will be complemented with a set of questions to challenge the students and a section for the instructors, concerning the results obtained and advice on getting the best outcome from the experiment. A section covering practical aspects with tips and advice for the instructors, together with the results obtained in the laboratory by students, has been compiled for each experiment. Targeted at professors and lecturers in chemistry, this useful text will provide up to date experiments putting the science into context for the students.

This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.

The second edition of the popular Chromatographic Integration Methods has been completely revised and updated. Written by an expert with many years' experience with two of the world's largest manufacturers of computing integrators, it has been expanded to include a new section on validation of integrators in response to regulatory requirements for quality and validation. A new literature survey, additional diagrams and Author Index have also been added.

Well illustrated and easily read, this is an excellent source book for those who wish to increase their understanding of integrators. Chromatographic Integration Methods describes and discusses both manual and electronic techniques used, with the aim of aiding analysts to obtain more data from their chromatograms, and assist them with understanding how integrators work so that results are never accepted unquestioningly. As with the first edition, this book will be welcomed by all those in the chromatography field, particularly those at the bench.

Microwave Chemistry has changed the way to work in chemical laboratories and is an established state-of-the-art technology to accelarate and enhance chemical processes. This book not only gives an overview of the technology, its historical development and theoretical background, but also presents its exceptionally broad spectrum of applications. Microwave Chemistry enables graduate students and scientist to learn and apply its methods successfully.

Blood science has become a cornerstone of multiple disciplines, including clinical chemistry, disease diagnosis, and therapeutic monitoring. Over the past decade, we have witnessed the advent of increasingly powerful proteomics technologies that allow greater fundamental insights into the blood proteome. These technological improvements have, in part, fuelled the quest for the discovery of novel blood-based biomarkers of disease. Serum/Plasma Proteomics: Methods and Protocols is a comprehensive resource of protocols for areas, pre-analytical through to analytical, of plasma and serum proteomics. Divided into five convenient sections, this detailed volume covers fractionation strategies for in-depth blood proteome analysis, defined procedures for blood collection, handling and storage, detailed protocols for performing both antibody-based and non-antibody based quantitative assays, proteome analysis of blood cell compartments, circulating nanomebraneous vesicles and blood-related fluids, and finally data management, statistical design, and bioinformatic challenges. This book, contributed to by leading experts in the field, provides a valuable foundation for the development and application of blood-based proteomics. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Serum/Plasma Proteomics: Methods and Protocols, with its well-honed methodologies, seeks to serve both professionals and investigators new to the field in an effort to further our knowledge of this fundamental science.

The International Multidisciplinary Microscopy Congress (INTERM2013) was organized on October 10-13, 2013. The aim of the congress was to bring together scientists from various branches to discuss the latest advances in the field of microscopy. The contents of the congress have been broadened to a more "interdisciplinary" scope, so as to allow all scientists working on related subjects to participate and present their work. These proceedings include 39 peer-reviewed technical papers, submitted by leading academic and research institutions from over 12 countries and representing some of the most cutting-edge research available. The 39 papers are grouped into the following sections: - Applications of Microscopy in the Physical Sciences - Applications of Microscopy in the Biological Sciences

As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.

The use of thermal and calorimetric methods has shown rapid growth over the past few decades, in an increasingly wide range of applications. The original text was published in 2001; since then there have been significant advances in various analytical techniques and their applications. This second edition supplies an up to date, concise and readable account of the principles, experimental apparatus and practical procedures used in thermal analysis and calorimetric methods of analysis. Written by experts in their field, brief accounts of the basic theory are reinforced with detailed technical advances and contemporary developments. Where appropriate, applications are used to highlight particular operating principles or methods of interpretation. As an important source of information for many levels of readership in a variety of areas, this book will be an aid for students and lecturers through to industrial and laboratory staff and consultants.

Confocal Microscopy: Methods and Protocols, Second Edition takes the researcher from the bench top through the imaging process, to the page. Protocols for the preparation of tissues from many model organisms including worms, flies and mice have been included as well as chapters on confocal imaging of living cells, three dimensional analysis, and the measurement and presentation of confocal images for publication. Emphasis has been placed on the laser scanning confocal microscope since this is still the instrument used for most routine applications. The current generation of modern confocal instruments produces optical sections of cells and tissues that are free of out-of-focus fluorescence with reduced chances of artifacts from the techniques of specimen preparation. This allows the imaging of living specimens and measurements of physiological events within cells. Confocal microscopy has become essential in many fields of contemporary biomedical research where a light microscope is required for imaging fluorescently labeled cells and tissues, especially cell biology, developmental biology, neurobiology, and pathology. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Confocal Microscopy: Methods and Protocols, Second Edition is aimed primarily, but not exclusively, at the novice user with pointers to more advanced techniques.

This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.

Now fully updated to cover recent developments, this book covers the closely related techniques of electron microprobe analysis (EMPA) and scanning electron microscopy (SEM) specifically from a geological viewpoint. Topics discussed include: principles of electron-target interactions, electron beam instrumentation, X-ray spectrometry, general principles of SEM image formation, production of X-ray 'maps' showing elemental distributions, procedures for qualitative and quantitative X-ray analysis (both energy-dispersive and wavelength-dispersive), the use of both 'true' electron microprobes and SEMs fitted with X-ray spectrometers, and practical matters such as sample preparation and treatment of results. Throughout, there is an emphasis on geological aspects not mentioned in similar books aimed at a more general readership. The book avoids unnecessary technical detail in order to be easily accessible, and forms an up-to-date text on EMPA and SEM for geological postgraduate and postdoctoral researchers, as well as those working in industrial laboratories.

This immensely practical guide to PIV provides a condensed, yet exhaustive guide to most of the information needed for experiments employing the technique. This second edition has updated chapters on the principles and extra information on microscopic, high-speed and three component measurements as well as a description of advanced evaluation techniques. What's more, the huge increase in the range of possible applications has been taken into account as the chapter describing these applications of the PIV technique has been expanded.

Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti c literature detailing speci c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin specimen preparation have appeared until this present work, rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.

An Introduction to Digital Photomicrography is written for the hobbyist and the neophyte who wants to take pictures through the microscope. The book includes a description of the parts of the microscope; how to use adjust lighting; types of digital cameras; controls for adjusting digital cameras; choosing a video camera and controls for videography.

"Modeling Nanoscale Imaging in Electron Microscopy" presents the recent advances that have been made using mathematical methods to resolve problems in microscopy. With improvements in hardware-based aberration software significantly expanding the nanoscale imaging capabilities of scanning transmission electron microscopes (STEM), these mathematical models can replace some labor intensive procedures used to operate and maintain STEMs. This book, the first in its field since 1998, will also cover such relevant concepts as superresolution techniques, special denoising methods, application of mathematical/statistical learning theory, and compressed sensing.

In addition to the traditional cytogenetics still used as the basic methodology for everyday clinical diagnosis, new molecular cytogenetic techniques provide a useful basis for routine diagnosis. Flourescence in situ hybridization (FISH) has become a standard technique, and comparative genomic hybridization (CGH), spectral karyotyping (SKY), and multi-color FISH have shown their potential for diagnostic purposes. Following a section on tissue culture, chromosome staining and basic information about karyotyping, nomenclature and quality standards, protocols of relevance for comprehensive cytogenetic diagnostics are presented.

Earlier efforts in the field of thermal analysis were concerned with the demonstration of the applicability of techniques to a broad spectrum of materials and to establish the relationship of such techniques with other more accepted methods. While such efforts will and should continue, the Third International Conference was unique in that the first standards were disclosed for differential thermal analysis. This was the culmination of the international, cooperative effort of the ICTA's Standardization Committee. The standards currently are available from the United State's National Bureau of Standards. Thus, thermal analysis can be considered to have attained its majority. Reali zation of full maturity can be expected in the near future. Inclusion of plenary lectures in these volumes represents a significant departure from previous Conferences. This change is the result of the ICTA's recognition of its educational responsibilities. In the Foreword of the Proceedings of the Second International Confer ence, Professor L. Berg expressed the hope that thermal methods of analysis would find wider application in science and technology. The citation above, together with the papers presented, indicate the fulfillment of this hope. Xerox Corporation C. B. Murphy Rochester, N.Y., U.S.A. President, ICTA 1968-1971 X Ill PREFACE For the past two decades thermoanalytical methods have reached a stage of considerable importance, which is particularly due to the developments in the area of instrumentation."

This volume is based on papers presented at the International Symposium on X-Ray Microscopy held at Brookhaven National Laboratory, Upton NY, August 31-September 4, 1987. Previous recent symposia on the sub ject were held in New York in 1979, Gottingen in 1983 and Taipei in 1986. Developments in x-ray microscopy continue at a rapid pace, with im portant advances in all major areas: x-ray sources, optics and components, and microscopes and imaging systems. Taken as a whole, the work pre sented here emphasizes three major directions: (a) improvements in the capability and image-quality of x-ray microscopy, expressed principally in systems attached to large, high-brightness x-ray sources; (b) greater access to x-ray microscopy, expressed chiefly in systems employing small, often pulsed, x-ray sources; and (c) increased rate of exploration of applications of x-ray microscopy. The number of papers presented at the symposium has roughly dou bled compared with that of its predecessors. While we are delighted at this growth as a manifestation of vitality and rapid growth of the field, we did have to ask the authors to limit the length of their papers and to submit them in camera-ready form. We thank the authors for their con tributions and for their efforts in adhering to the guidelines on manuscript preparation.