This book provides an in-depth description of x-ray microanalysis in the electron microscope. It is sufficiently detailed to ensure that novices will understand the nuances of high-quality EDX analysis. Includes information about hardware design as well as the physics of x-ray generation, absorption and detection, and most post-detection data processing. Details on electron optics and electron probe formation allow the novice to make sensible adjustments to the electron microscope in order to set up a system which optimises analysis. It also helps the reader determine which microanalytical method is more suitable for their planned application.

"Veterinary Laboratory Medicine" covers all aspects of basic clinical biochemistry and haematology, and includes test-by-test interpretation of laboratory results. Information is provided on sampling techniques, the selection and use of an external laboratory, as well as near-patient testing and the practice laboratory. Also included are step-by-step instructions for most commonly used point-of-care tests, a guide to the evaluation of instruments for in-practice use, and a detailed explanation of the principles of impedance counting and photometric analysis. The book will be ideal for practitioners who require a guide to laboratory work, and for veterinary students studying laboratory medicine and clinical pathology.

The second edition has been fully updated to reflect advances in diagnostic techniques, and includes new chapters on diagnostic endocrinology and feline virus testing as well as a much expanded chapter on diagnostic profiling and pattern recognition.

The latest version of a bestseller, Hazardous Laboratory Chemicals Disposal Guide, Third Edition includes individual entries for over 300 compounds. The extensive list of references has been updated and includes entries for 15 pesticides commonly used in greenhouses. Emphasis is placed on disposal methods that turn hazardous waste material into non-toxic products. These methods fall into several categories, including acid/base neutralization, oxidation or reduction, and precipitation of toxic ions as insoluble solids. The text also provides data on hazardous reactions of chemicals, assisting laboratory managers in developing a plan of action for emergencies such as the spill of any of the chemicals listed.

Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

Super-Resolution Microscopy Techniques in the Neurosciences serves as a comprehensive description of current super-resolution techniques, including the physical principles that allowed for their development, some of the most recent neurobiological applications and selected information for the practical use of these technologies. Written for the Neuromethods series, this detailed work contains contributions from experts in the field and provides key implementation advice to ensure successful results in the lab. Authoritative and cutting-edge, Super-Resolution Microscopy Techniques in the Neurosciences is an ideal guide for researchers aiming to continue increasing the resolution in the imaging capabilities of neuroscientists and thereby changing the perspective in which cellular biology processes are understood at the nanometer scale.

Aberration-Corrected Imaging in Transmission Electron Microscopy provides an introduction to aberration-corrected atomic-resolution electron microscopy imaging in materials and physical sciences. It covers both the broad beam transmission mode (TEM; transmission electron microscopy) and the scanning transmission mode (STEM; scanning transmission electron microscopy). The book is structured in three parts. The first part introduces the basics of conventional atomic-resolution electron microscopy imaging in TEM and STEM modes. This part also describes limits of conventional electron microscopes and possible artefacts which are caused by the intrinsic lens aberrations that are unavoidable in such instruments. The second part introduces fundamental electron optical concepts and thus provides a brief introduction to electron optics. Based on the first and second parts of the book, the third part focuses on aberration correction; it describes the various aberrations in electron microscopy and introduces the concepts of spherical aberration correctors and advanced aberration correctors, including correctors for chromatic aberration. This part also provides guidelines on how to optimize the imaging conditions for atomic-resolution STEM and TEM imaging.This second edition has been completely revised and updated in order to incorporate the very recent technological and scientific achievements that have been realized since the first edition appeared in 2010.

The knowledge base of chromatography continued to expand throughout the 1990s owing to its many applications to problems of contemporary interest in industry, life and environmental sciences. Organizing this information into a single text for a diverse group of scientists has become increasingly difficult. The present book stemmed from the desire to revise Chromatography Today, written by the same author with Salwa K. Poole, and published in 1991. This title is considered to be one of the definitive texts on chromatography. It was soon realized however, that a simple revision would not provide the desired result of a contemporary picture of the practice of chromatography at the turn of the century. The only workable solution was to start afresh, maintaining the same general philosophy and concept for Chromatography Today where possible, while creating essentially a new book.
The format of the new book is modular, with extensive cross-references to permit rapid location of related material using different separation concepts. Important features are extensive tabulation of essential data for performing separations and an extensive bibliography to the most recent literature.
This title is intended as a suitable text for graduate level courses in the separation sciences and as a self-study guide for professional chromatographers wishing to refresh their background in this rapidly expanding field.
The Essence of Chromatography presents a comprehensive survey of modern chromatography and is an effective replacement for Chromatography Today.
.Comprehensive and authoritative coverage of chromatographic techniques
.Contains extensive coverage of recent literature on this subject
.Ideal text for graduates and suitable for professional chromatographers"

A lab manual for the General Chemistry course, Beran has been popular for the past nine editions because of its broad selection of experiments, clear layout, and design. Containing enough material for two or three terms, this lab manual emphasizes chemical principles as well as techniques. In addition, the manual helps students understand the timing and situations for various techniques.

We are now accustomed to conceive of science as an instrumental activity, producing numbers, measurements and graphs by means of sophisticated devices. This book investigates the historical process that gave rise to this instrumental culture. The contributors trace the displacement of instruments across the globe, the spread of practices or precision and the circulation and appropriation of skills and knowledge.
Through comparative and contextual approaches, the volume confronts the tension between the local and the global, examining the process of the universalization of science. Bringing together case studies ranging from the seventeenth to the twentieth centuries, contributors discuss French, German and British initiatives, as well as the knowledge and techniques of travellers in countries such as India, Africa, South East Asia and the Americas.
Students and researchers interested in the history of science in both Western and non-Western cultures will find this book a valuable and thought-provoking read.

eBook available with sample pages: 0203219015

This book is published on behalf of the Biological Stain Commission.
For 75 years "Conn's Biological Stains "has been a standard reference for all those who used dyes and colorants in the biological and medical sciences. This long-awaited Tenth Edition appears 25 years after R.D. Lillie's Ninth Edition and has been completely rewritten to reflect the increase in range of uses. Although the staining of microscopical preparations continues to expand the uses of dyes and fluorochromes now extend far beyond this traditional application.
This book provides the first critical overview of the whole range of low molecular weight fluorescent probes, outside the catalog literature. The first ten chapters are essays, by leading experts, on the important aspects of colorants and their uses. Most of the remainder of the book consists of descriptions by Dr. Horobin of the properties and recent applications of hundreds of individual compounds, in about twenty chemical classes. The last chapter reviews the procedures employed at the Biological Stain Commission's laboratory to assay and test dyes and certify them as suitable for their intended applications.

This book is divided into two parts. In the first part we introduce the foundations of special relativity, such as, the inertial frame of reference, the definition of simulataneity, and Einstein's two basic hypotheses. We give the main relativistic effects, e.g. the relativity of simultaneity, velocity addition, length-contraction, the apparent shape of a moving body, time-dilation, Doppler effect, and the Thomas precession, In particular, the simultaneity problem and slow transport of clocks are investigated in detail by means of the test theories of special relativity. In the second part, variant types of experiments performed up to now are analyzed and compared to the predictions of special relativity. This shows that the experiments are a test of the two-way speed of light, but not of the one-way speed of light.

The goal of an activity-directed isolation process is to isolate bioactive compounds which may provide structural leads of therapeutic importance. Whereas the traditional process of drug development is long and expensive, simple and rapid bioassays can serve as the starting point for drug discovery. This book presents a range of "bench top" bioassay techniques useful for natural product and pharmaceutical chemists involved in drug discovery and pharmacognosy. The contents detail a number of enzyme-based assays, cell-based functional bioassays and receptor radioligand binding assays along with detailed descriptions of each type. The majority of these bioassays are presented in a step-by-step format, so they could even be implemented by technical personnel with little background in microbiology, biochemistry or pharmacology.

Electron Energy Loss Spectroscopy (EELS) is a high resolution technique used for the analysis of thin samples of material. The technique is used in many modern transmission electron microscopes to characterise materials. This book provides an up-to-date introduction to the principles and applications of EELS. Specific topics covered include, theory of EELS, elemental quantification, EELS fine structure, EELS imaging and advanced techniques.

An introductory level guide to electron diffraction in the TEM - This book is a practical guide to electron diffraction in the transmission electron microscope (TEM). Case studies and examples are used to provide an invaluable introduction to the subject for those new to the technique. The book explains the basic methods used to obtain diffraction patterns with the TEM. The numerous illustrations aid the understanding of the conclusions reached. * Gives useful case studies and examples to guide the new user * User-friendly approach with simple explanations and informative illustrations Explains the basic methods of how to obtain images and patterns with the TEM, and how to interpret them. TAMs are used in many labs, and allow users to explore the structure of a specimen of interest (e.g. a section through a particular material, crystal or tissue). The TEM is used to pass electrons through a sample, and the electrons are scattered or diffracted in order to produce an image. This book looks at the patterns produced by diffracted electrons to study the appearance, orientation and structure of the sample.

An outstanding practical guide to the most common chemometric methods in use today

Chemometrics explains how to apply the most widely used pattern recognition and multivariate calibration techniques to solve data analysis problems. This practical guide describes all key methods in terms of processes and applications in order to help the reader easily identify the best technique for a given situation.

Drawing on years of industrial experience with chemometric tools, the authors share their six basic steps, or "habits," for achieving reliable chemometric results, and cover key areas such as:

• Defining and understanding the problem
• Experimental planning and design
• Preprocessing of samples and variables
• Supervised and unsupervised pattern recognition
• Classical and inverse methods of multivariate calibration

Complete with helpful chapter-end summaries, technical references, and more, this book is an invaluable hands-on resource for analytical chemists and laboratory scientists who use chemometrics in their work.

The first edition of this classic book remains one of the very few introductory books covering both theoretical and practical aspects of thermal analysis (TA). This new edition includes a much enlarged section on MDSC, in which the instrument is described and a critical appraisal of the technique presented. Other additions include new sections on rate-controlled TGA, OTTER, and Specific Heat Spectroscopy, and a thoroughly updated section on X-Ray DSC. This very practical book is a must for people who use thermal analysis techniques in their everyday work. "An excellent introductory text" — Review of 1st Edition.

With IC technology continuing to advance, the analysis of very small structures remains critically important. Microscopy of Semiconducting Materials provides an overview of advances in semiconductor studies using microscopy. The book explores the use of transmission and scanning electron microscopy, ultrafine electron probes, and EELS to investigate semiconducting structures. It also covers specimen preparation using focused ion beam milling and advances in microscopy techniques using different types of scanning probes, such as AFM, STM, and SCM. In addition, the book discusses a range of materials, from finished devices to partly processed materials and structures, including nanoscale wires and dots.
This volume provides an authoritative reference for all academics and researchers in materials science, electrical and electronic engineering and instrumentation, and condensed matter physics.

Water is the most basic need of mankind. Drinking water is considered the most essential use of water in life. Therefore it must be free of pathogens, toxins and carcinogens.

Absolutley pure water does not exist in nature. Surface water absorbs particles, carbon dioxide and other gases and mixes with silt and inorganic matters from the environment. When treated and untreated domestic and industrial waste is discharged into natural bodies of water the situation becomes even more complex. Thus human waste, drinking water and communicable diseases are directly related.

Water contamination is measured by the level of pollutants present in a sample. Regular analytical estimation of wastewater is the answer. This manual emphasizes the importance of water purity for drinking and domestic purposes, different types of water and their utilization in various activities, the water quality requirements and criteria of International and Governmental Agencies, and simple estimation procedures and the significance of each analytical test.

Quality Assessment of Water and Wastewater describes methods for ascertaining the quality and contamination levels of waters from a range of sources like ground, surface, potable water supplies, marine, beaches, swimming pools and other recreational facilities, and domestic and industrial wastewater. It includes important derivatives used in the preparation of standard solutions, data analysis, interpretation and units of expressions of the results. It also discusses all major pollutants - their origins and impact on the environment and health - with the basic chemistry of their analysis and complete methodology explained systematically.

Basic principles of applied life sciences such as recombinant DNA technology is used in most life sciences industries marketing bio-formulations for designing more effective protein-based drugs, such as erythropoietin and fast-acting insulin etc. In recent times genetically engineered host cells from mammal, animal and plants are also being used in life sciences industries to manufacture biologics. This book discusses the most basic as well advanced issues on biological products for successfully managing a life sciences industry. It elucidates the life cycle of biological molecules, right from the conceptual development of different types of biopolymers, and their subsequent transfer from the conical flasks in laboratory to life sciences industries for large scale production and marketing. It focuses on sustainable longevity in the life cycle of commercial biopolymers. Cumulative facts and figures in this volume would immensely help in inspiring life sciences industry promoters to monitor value chain transfer process of biologics for better profitability. Additionally, it would serve as a perusal document for the students and researchers interested in entrepreneurial ventures or having their own start-up projects for the commercialization of biologics.

The central role of RNA in many cellular processes, in biotechnology, and as pharmaceutical agents, has created an interest in experimental methods applied to RNA molecules. This book provides scientists with a comprehensive collection of thoroughly tested up-to-date manuals for investigating RNA-protein complexes "in vitro." The protocols can be performed by researchers trained in standard molecular biological techniques and require a minimum of specialized equipment. The procedures include recommendation of suppliers of reagents.

The central role of RNA in many cellular processes, in biotechnology, and as pharmaceutical agents, has created an interest in experimental methods applied to RNA molecules. This book provides scientists with a comprehensive collection of thoroughly tested up-to-date manuals for investigating RNA-protein complexes "in vitro." The protocols can be performed by researchers trained in standard molecular biological techniques and require a minimum of specialized equipment. The procedures include recommendation of suppliers of reagents.

This volume describes why a microscope is necessary, the interactions of light with matter and the use of the stereo low-power microscope. It examines the high-power compound microscope, detailing both the older stands with separate lamp, as well as the latest research models with integral illumination and infinity-corrected optics.

The new edition of this widely-used sourcebook details the startlingly array of diagnostic equipment available in the medical laboratory of the nineties, and also covers maintenance and quality assurance for each type of instrument. This book includes 17 completely rewritten chapters and 7 new ones, on nephelometry and turbidimetry, gas chromatography, mass spectrometry, flow cytometry, automated immunoassay systems, automated blood bank systems, and physician's office laboratory instrumentation.

Series Information:
Garland Encyclopedias in the History of Science

This practical handbook provides an introduction for all scanning transmission electron microscopy (STEM) users, but with complete background information concerning features and advantages of the technology. The text is without rigorous mathematical treatment of the fundamentals of STEM, yet comprehensive in its qualitative descriptions.