Originally published in 1941, this book seeks to inform the scientific community of the possible uses of photography in research or teaching. Lawrence gives an account of the possibilities and limitations of the medium for the scientific reader as well as for the 'serious amateur', explains the various mechanisms of the camera and gives some suggestions for scientific applications, such as high-speed photography. This book will be of value to anyone with an interest in photography or the history of science.

This book is both an introduction and a demonstration of how Visual Basic for Applications (VBA) can greatly enhance Microsoft Excel (R) by giving users the ability to create their own functions within a worksheet and to create subroutines to perform repetitive actions. The book is written so readers are encouraged to experiment with VBA programming with examples using fairly simple physics or non-complicated mathematics such as root finding and numerical integration. Tested Excel (R) workbooks are available for each chapter and there is nothing to buy or install.

Providing the knowledge and practical experience to begin analysing scientific data, this book is ideal for physical sciences students wishing to improve their data handling skills. The book focuses on explaining and developing the practice and understanding of basic statistical analysis, concentrating on a few core ideas, such as the visual display of information, modelling using the likelihood function, and simulating random data. Key concepts are developed through a combination of graphical explanations, worked examples, example computer code and case studies using real data. Students will develop an understanding of the ideas behind statistical methods and gain experience in applying them in practice. Further resources are available at www.cambridge.org/9781107607590, including data files for the case studies so students can practise analysing data, and exercises to test students' understanding.

As this book. Antibacterial Peptide Protocols, will attest, my enthusi asm for the field of antibacterial peptides is based on a conviction (and I am unashamed to say, prejudice) that these substances are in essence antibiotics produced by the host that then participate in host defense against infectious agents. Because of their capacity to exert antibiotic-like action against patho genic microorganisms (bacteria, fungi, parasites, and viruses), there is reason to believe that these agents will soon be used clinically to treat infectious diseases. In fact, in recent years, biotechnology companies have been formed for the sole purpose of developing antibacterial peptides for clinical use. It should be emphasized that antibacterial peptides will likely play a major role in the treatment of infectious diseases, particularly with the increasing prob lem of multidrug-resistant microbes and the relative dearth of new antibiotics being provided by pharmaceutical companies. The topic of this volume of Methods in Molecular Biology, the diverse methods used in research on antibacterial peptides, is thus quite timely. As the subject of antibacterial peptides develops into its own discipline (something strongly suggested by the explosion in the number of papers published over the past decade), it is essential that reliable techniques and strategies be made available not only to those of us in the field, but also to the newcomers and researchers in complementary disciplines."

This volume contains papers presented at the NATO Advanced Research Workshop (ARW) on Photons and Local Probes. The workshop had two predecessors. The first was the NATO ARW on Near Field Optics, held in October 1992 at Arc et Senans and was organized by Daniel Courjon and Dieter Pohl. The other predecessor was a workshop on Photons and Scanning Probe Microscopies held at the University of Konstanz in July 1992. The workshop on Photons and Local Probes was held at the Loechnerhaus on the Reichenau Island at the Lake of Constance, from September 11 to 17, 1994. The Reichenau Island was an important place in Europe in the middle age. Even the tomb of one of the carolingian emperors, Charles the Fat, is located there. At this workshop more than 60 scientists from Europe and the United States met to communicate their latest results in the field of local probes in combination with optical techniques. In eight sessions 31 talks as well as 9 posters were presented. Among those 31 publications were submitted for publication in the NATO proceedings. They were accepted after a strict, but constructive refereeing process.

Scattering experiments, using X-ray, light and neutron sources (in historical order) are key techniques for studying structure and dynamics in systems containing colliods, polymers, surfactants and biological macromolecules, summarized here as soft condensed matter. The education in this field in Europe is very heterogeneous and frequently inadequate, which severely limits an efficient use of these methods, especially at large-scale facilities. The series of "Bombannes" schools and the completely revised and updated second edition of the lecture notes are devoted to a practical approach to current methodology of static and dynamic techiques. Basic information on data interpretation, on the complementarity of the different types of radiation, as well as information on recent applications and developments is presented. The aim is to avoid over - as well as under-exploitation of data.

Synchrotron radiation has been a revolutionary and invaluable research tool for a wide range of scientists, including chemists, biologists, physicists, materials scientists, geophysicists. It has also found multidisciplinary applications with problems ranging from archeology through cultural heritage to paleontology. The subject of this book is x-ray spectroscopy using synchrotron radiation, and the target audience is both current and potential users of synchrotron facilities. The first half of the book introduces readers to the fundamentals of storage ring operations, the qualities of the synchrotron radiation produced, the x-ray optics required to transport this radiation, and the detectors used for measurements. The second half of the book describes the important spectroscopic techniques that use synchrotron x-rays, including chapters on x-ray absorption, x-ray fluorescence, resonant and non-resonant inelastic x-ray scattering, nuclear spectroscopies, and x-ray photoemission. A final chapter surveys the exciting developments of free electron laser sources, which promise a second revolution in x-ray science. Thanks to the detailed descriptions in the book, prospective users will be able to quickly begin working with these techniques. Experienced users will find useful summaries, key equations, and exhaustive references to key papers in the field, as well as outlines of the historical developments in the field. Along with plentiful illustrations, this work includes access to supplemental Mathematica notebooks, which can be used for some of the more complex calculations and as a teaching aid. This book should appeal to graduate students, postdoctoral researchers, and senior scientists alike.

This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response. Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field.

The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal-fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis.

This updated and revised edition of a classic work provides a summary of methods for numerical computation of high resolution conventional and scanning transmission electron microscope images. At the limits of resolution, image artifacts due to the instrument and the specimen interaction can complicate image interpretation. Image calculations can help the user to interpret and understand high resolution information in recorded electron micrographs. The book contains expanded sections on aberration correction, including a detailed discussion of higher order (multipole) aberrations and their effect on high resolution imaging, new imaging modes such as ABF (annular bright field), and the latest developments in parallel processing using GPUs (graphic processing units), as well as updated references. Beginning and experienced users at the advanced undergraduate or graduate level will find the book to be a unique and essential guide to the theory and methods of computation in electron microscopy.

As a response to the climate crisis and its effect on marine ecosystems and coastal populations, this book proposes concrete science driven solutions at establishing transformation pathways towards Sustainable Blue Growth, that are supported by technically and socially innovative innovations. This book proposes investment options and management solutions that have the potential of making our seas and oceans resilient to crises- climate, financial, health- by laying the foundations for a green/blue, circular economy that is anchored in science driven solutions and geared toward public well-being. Now is the time to usher in systemic economic change and the good news is that we have our blueprint: it's the combination of UN Agenda 2030 (17 SDG) and European Commission's European Green Deal! There is no doubt that the Earth's survival will depend on the protection and sustainable management of our seas and oceans and the resources they provide. This is recognized by the Joint Communication on International Ocean Governance, which is an integral part of the EU's response to the United Nations' 2030 Agenda for Sustainable Development, and in particular to the targets set out by Sustainable Development Goal 14 (SDG 14) to "conserve and sustainably use the oceans, seas and marine resources". The analytical framework and science-driven concrete management solutions proposed in this book can accelerate the transition to a sustainable management of our seas and oceans, by turning the current challenges into opportunities for sustainable economic growth which is both environmentally resilient and leaves no one behind.

Superconducting devices, which can carry huge currents and generate strong magnetic fields without losing energy, are improving at a tremendous pace. This book provides a modern, up-to-date reference on both the physics and the technology of superconducting magnets. It is unique in combining the theoretical aspects of superconductivity, electromagnetic field theory, and the thermodynamics of helium cooling with the technological details of producing and engineering high performance superconducting materials. The book provides the reliable, expert advice for designing, manufacturing, and testing complex high field superconducting magnets of predictable performance, and it places particular emphasis on beam transport and accelerator magnets in high energy particle physics.

Passive acoustic monitoring is increasingly used by the scientific community to study, survey and census marine mammals, especially cetaceans, many of which are easier to hear than to see. PAM is also used to support efforts to mitigate potential negative effects of human activities such as ship traffic, military and civilian sonar and offshore exploration. Walter Zimmer provides an integrated approach to PAM, combining physical principles, discussion of technical tools and application-oriented concepts of operations. Additionally, relevant information and tools necessary to assess existing and future PAM systems are presented, with Matlab code used to generate figures and results so readers can reproduce data and modify code to analyse the impact of changes. This allows the principles to be studied whilst discovering potential difficulties and side effects. Aimed at graduate students and researchers, the book provides all information and tools necessary to gain a comprehensive understanding of this interdisciplinary subject.

The Transmission Electron Microscope (TEM) is the ultimate tool to see and measure structures on the nanoscale and to probe their elemental composition and electronic structure with sub-nanometer spatial resolution. Recent technological breakthroughs have revolutionized our understanding of materials via use of the TEM, and it promises to become a significant tool in understanding biological and biomolecular systems such as viruses and DNA molecules. This book is a practical guide for scientists who need to use the TEM as a tool to answer questions about physical and chemical phenomena on the nanoscale.

A zebrafish, the hull of a miniature ship, a mathematical equation and a food chain - what do these things have in common? They are examples of models used by scientists to isolate and study particular aspects of the world around us. This book begins by introducing the concept of a scientific model from an intuitive perspective, drawing parallels to mental models and artistic representations. It then recounts the history of modelling from the 16th century up until the present day. The iterative process of model building is described and discussed in the context of complex models with high predictive accuracy versus simpler models that provide more of a conceptual understanding. To illustrate the diversity of opinions within the scientific community, we also present the results of an interview study, in which ten scientists from different disciplines describe their views on modelling and how models feature in their work. Lastly, it includes a number of worked examples that span different modelling approaches and techniques. It provides a comprehensive introduction to scientific models and shows how models are constructed and used in modern science. It also addresses the approach to, and the culture surrounding modelling in different scientific disciplines. It serves as an inspiration for model building and also facilitates interdisciplinary collaborations by showing how models are used in different scientific fields. The book is aimed primarily at students in the sciences and engineering, as well as students at teacher training colleges but will also appeal to interested readers wanting to get an overview of scientific modelling in general and different modelling approaches in particular.

The past three decades have witnessed the great success of lithium-ion batteries, especially in the areas of 3C products, electrical vehicles, and smart grid applications. However, further optimization of the energy/power density, coulombic efficiency, cycle life, charge speed, and environmental adaptability are still needed. To address these issues, a thorough understanding of the reaction inside a battery or dynamic evolution of each component is required. Microscopy and Microanalysis for Lithium-Ion Batteries discusses advanced analytical techniques that offer the capability of resolving the structure and chemistry at an atomic resolution to further drive lithium-ion battery research and development. * Provides comprehensive techniques that probe the fundamentals of Li-ion batteries. * Covers the basic principles of the techniques involved as well as its application in battery research. * Describes details of experimental set-ups and procedure for successful experiments. This reference is aimed at researchers, engineers, and scientists studying lithium-ion batteries including chemical, materials, and electrical engineers, as well as chemists and physicists.

A comprehensive, self-contained primer on validated numerics This textbook provides a comprehensive introduction to the theory and practice of validated numerics, an emerging new field that combines the strengths of scientific computing and pure mathematics. In numerous fields ranging from pharmaceutics and engineering to weather prediction and robotics, fast and precise computations are essential. Based on the theory of set-valued analysis, a new suite of numerical methods is developed, producing efficient and reliable solvers for numerous problems in nonlinear analysis. Validated numerics yields rigorous computations that can find all possible solutions to a problem while taking into account all possible sources of error-fast, and with guaranteed accuracy. Validated Numerics offers a self-contained primer on the subject, guiding readers from the basics to more advanced concepts and techniques. This book is an essential resource for those entering this fast-developing field, and it is also the ideal textbook for graduate students and advanced undergraduates needing an accessible introduction to the subject. Validated Numerics features many examples, exercises, and computer labs using MATLAB/C++, as well as detailed appendixes and an extensive bibliography for further reading. Provides a comprehensive, self-contained introduction to validated numerics Requires no advanced mathematics or programming skills Features many examples, exercises, and computer labs Includes code snippets that illustrate implementation Suitable as a textbook for graduate students and advanced undergraduates

Practical NMR Spectroscopy Laboratory Guide is designed to provide non-expert NMR users, typically graduate students in chemistry, an introduction to various facets of practical solution-state NMR spectroscopy. Each chapter offers a series of hands-on exercises, introducing various NMR concepts and experiments and guiding the reader in running these experiments using an NMR spectrometer. The book is written for use with a Bruker NMR spectrometer running TopSpin software versions 1 or 2. This practical resource functions both as a text for instructors of a practical NMR course and also as a reference for spectrometer administrators or NMR facility directors when doing user training. This guide serves as serve as excellent, practical resource on its own or as a companion book to Timothy Claridge's High-Resolution NMR Techniques in Organic Chemistry, 2nd Edition (Elsevier, 2009).

This volume is the first of its kind on focusing gamma-ray telescopes. Forty-eight refereed papers provide a comprehensive overview of the scientific potential and technical challenges of this nascent tool for nuclear astrophysics. The book features articles dealing with pivotal technologies such as grazing incident mirrors, multilayer coatings, Laue- and Fresnel-lenses - and even an optic using the curvature of space-time.

This spectacularly illustrated book celebrates the structural beauty of everyday materials and the space-age technologies used to probe their surface features and internal structures. It introduces the reader to the various instruments and their uses: scanning electron, ion, and tunneling microscopies, acoustic microscopy and transmission electron microscopy. The book describes how images are processed and analyzed, and how modern materials science is based on these techniques and their ability to "see" materials at the atomic level. The book includes hundreds of illustrations and 32 pages of beautiful color plates depicting the complex microscopic realm within such everyday materials as the metals used in cars and planes, polymer fabrics, ceramics, and the ubiquitous silicon semiconductors, without which society today would fall into disarray and confusion. The many full-color and black-and-white illustrations make this book a pleasure for the eye, in addition to being a useful resource for scientists, students, researchers, and engineers involved in solid-state physics, materials science, geology, and chemistry.

This book focuses on charged-particle optics and microscopy, as well as their applications in the materials sciences. Presenting a range of cutting-edge theoretical and methodological advances in electron microscopy and microanalysis, and examining their crucial roles in modern materials research, it offers a unique resource for all researchers who work in ultramicroscopy and/or materials research. The book addresses the growing opportunities in this field and introduces readers to the state of the art in charged-particle microscopy techniques. It showcases recent advances in scanning electron microscopy, transmission electron microscopy and helium ion microscopy, including advanced spectroscopy, spherical-corrected microscopy, focused-ion imaging and in-situ microscopy. Covering these and other essential topics, the book is intended to facilitate the development of microscopy techniques, inspire young researchers, and make a valuable contribution to the field.

The 3rd International Multidisciplinary Microscopy Congress (InterM2015), held from 19 to 23 October 2015, focused on the latest developments concerning applications of microscopy in the biological, physical and chemical sciences at all dimensional scales, advances in instrumentation, techniques in and educational materials on microscopy. These proceedings gather 17 peer-reviewed technical papers submitted by leading academic and research institutions from nine countries and representing some of the most cutting-edge research available.

Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.