This book is both an introduction and a demonstration of how Visual Basic for Applications (VBA) can greatly enhance Microsoft Excel (R) by giving users the ability to create their own functions within a worksheet and to create subroutines to perform repetitive actions. The book is written so readers are encouraged to experiment with VBA programming with examples using fairly simple physics or non-complicated mathematics such as root finding and numerical integration. Tested Excel (R) workbooks are available for each chapter and there is nothing to buy or install.

'Although the study of such defects is regularly examined at length in more general books on electron microscopy, this text in which they are centre-stage will surely be appreciated.' [Read Full Review]UltramicroscopyThis unique reference text provides those who are studying crystal lattice defects using a transmission electron microscope (TEM) with a basic knowledge of transmission electron microscopy. As it has been written for beginners, the principles of both transmission electron microscopy and crystallography have been clearly and simply explained, with the use of many figures and photographs to aid understanding. Mathematics is avoided where possible, and problems and exercises are amply provided.

This updated and revised edition of a classic work provides a summary of methods for numerical computation of high resolution conventional and scanning transmission electron microscope images. At the limits of resolution, image artifacts due to the instrument and the specimen interaction can complicate image interpretation. Image calculations can help the user to interpret and understand high resolution information in recorded electron micrographs. The book contains expanded sections on aberration correction, including a detailed discussion of higher order (multipole) aberrations and their effect on high resolution imaging, new imaging modes such as ABF (annular bright field), and the latest developments in parallel processing using GPUs (graphic processing units), as well as updated references. Beginning and experienced users at the advanced undergraduate or graduate level will find the book to be a unique and essential guide to the theory and methods of computation in electron microscopy.

As this book. Antibacterial Peptide Protocols, will attest, my enthusi asm for the field of antibacterial peptides is based on a conviction (and I am unashamed to say, prejudice) that these substances are in essence antibiotics produced by the host that then participate in host defense against infectious agents. Because of their capacity to exert antibiotic-like action against patho genic microorganisms (bacteria, fungi, parasites, and viruses), there is reason to believe that these agents will soon be used clinically to treat infectious diseases. In fact, in recent years, biotechnology companies have been formed for the sole purpose of developing antibacterial peptides for clinical use. It should be emphasized that antibacterial peptides will likely play a major role in the treatment of infectious diseases, particularly with the increasing prob lem of multidrug-resistant microbes and the relative dearth of new antibiotics being provided by pharmaceutical companies. The topic of this volume of Methods in Molecular Biology, the diverse methods used in research on antibacterial peptides, is thus quite timely. As the subject of antibacterial peptides develops into its own discipline (something strongly suggested by the explosion in the number of papers published over the past decade), it is essential that reliable techniques and strategies be made available not only to those of us in the field, but also to the newcomers and researchers in complementary disciplines."

This volume contains papers presented at the NATO Advanced Research Workshop (ARW) on Photons and Local Probes. The workshop had two predecessors. The first was the NATO ARW on Near Field Optics, held in October 1992 at Arc et Senans and was organized by Daniel Courjon and Dieter Pohl. The other predecessor was a workshop on Photons and Scanning Probe Microscopies held at the University of Konstanz in July 1992. The workshop on Photons and Local Probes was held at the Loechnerhaus on the Reichenau Island at the Lake of Constance, from September 11 to 17, 1994. The Reichenau Island was an important place in Europe in the middle age. Even the tomb of one of the carolingian emperors, Charles the Fat, is located there. At this workshop more than 60 scientists from Europe and the United States met to communicate their latest results in the field of local probes in combination with optical techniques. In eight sessions 31 talks as well as 9 posters were presented. Among those 31 publications were submitted for publication in the NATO proceedings. They were accepted after a strict, but constructive refereeing process.

Radioisotopes are invaluable tools for research in the biosciences. They provide unrivalled sensitivity for the detection and identification of biological molecules, facilitating for example drug discovery and human genetics.The book helps the research scientist to understand what is involved in the use of radioactive compounds and provides protocols for their use. Advice on legislation, guidance on safe handling and detailed recipes are provided.

Scattering experiments, using X-ray, light and neutron sources (in historical order) are key techniques for studying structure and dynamics in systems containing colliods, polymers, surfactants and biological macromolecules, summarized here as soft condensed matter. The education in this field in Europe is very heterogeneous and frequently inadequate, which severely limits an efficient use of these methods, especially at large-scale facilities. The series of "Bombannes" schools and the completely revised and updated second edition of the lecture notes are devoted to a practical approach to current methodology of static and dynamic techiques. Basic information on data interpretation, on the complementarity of the different types of radiation, as well as information on recent applications and developments is presented. The aim is to avoid over - as well as under-exploitation of data.

The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal-fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis.

Synchrotron radiation has been a revolutionary and invaluable research tool for a wide range of scientists, including chemists, biologists, physicists, materials scientists, geophysicists. It has also found multidisciplinary applications with problems ranging from archeology through cultural heritage to paleontology. The subject of this book is x-ray spectroscopy using synchrotron radiation, and the target audience is both current and potential users of synchrotron facilities. The first half of the book introduces readers to the fundamentals of storage ring operations, the qualities of the synchrotron radiation produced, the x-ray optics required to transport this radiation, and the detectors used for measurements. The second half of the book describes the important spectroscopic techniques that use synchrotron x-rays, including chapters on x-ray absorption, x-ray fluorescence, resonant and non-resonant inelastic x-ray scattering, nuclear spectroscopies, and x-ray photoemission. A final chapter surveys the exciting developments of free electron laser sources, which promise a second revolution in x-ray science. Thanks to the detailed descriptions in the book, prospective users will be able to quickly begin working with these techniques. Experienced users will find useful summaries, key equations, and exhaustive references to key papers in the field, as well as outlines of the historical developments in the field. Along with plentiful illustrations, this work includes access to supplemental Mathematica notebooks, which can be used for some of the more complex calculations and as a teaching aid. This book should appeal to graduate students, postdoctoral researchers, and senior scientists alike.

Spark scientific curiosity from a young age with this six-level course through an enquiry-based approach and active learning. Collins International Primary Science fully meets the requirements of the Cambridge Primary Science Curriculum Framework from 2020 and has been carefully developed for a range of international contexts. The course is organised into four main strands: Biology, Chemistry, Physics and Earth and Space and the skills detailed under the 'Thinking and Working Scientifically' strand are introduced and taught in the context of those areas. For each Student's Book at Stages 1 to 6, we offer: A full colour and highly illustrated Student's Book Photo-rich spreads show that science is 'real' and puts it into context Earth and Space content covers the new curriculum framework Thinking and Working Scientifically deepens and enhances the delivery of Science skills Actively learn through practical activities that don't require specialist equipment or labs Scaffolding allows students of varying abilities to work with common content and meet learning objectives Supports Cambridge Global Perspectives (TM) with activities that develop and practise key skills Provides learner support as part of a set of resources for the Cambridge Primary Science curriculum framework (0097) from 2020 This series is endorsed by Cambridge Assessment International Education to support the new curriculum framework 0097 from 2020.

Many chemists - especially those most brilliant in their field - fail to appreciate the power of planned experimentation. They dislike the mathematical aspects of statistical analysis. In addition, these otherwise very capable chemists also dismissed predictive models based only on empirical data. Ironically, in the hands of subject matter experts like these elite chemists, the statistical methods of mixture design and analysis provide the means for rapidly converging on optimal compositions. What differentiates Formulation Simplified from the standard statistical texts on mixture design is that the authors make the topic relatively easy and fun to read. They provide a whole new collection of insighful original studies that illustrate the essentials of mixture design and analysis. Solid industrial examples are offered as problems at the end of many chapters for those who are serious about trying new tools on their own. Statistical software to do the computations can be freely accessed via a web site developed in support of this book.

This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response. Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field.

This book contains up-to-date methods for all aspects of breeding and studying mice as a model for the way that genes work to control development, physiology and behaviour. The mouse has recently become the model organism for human genetics. The chapters are written by experts in the field and aimed both at an audience of scientists new to the field, and those who want an overview of the latest techniques.

X-ray crystallography is the major method of determining biological structures yet the procedures involved in obtaining the required crystals are still seen as something of a black art by many molecular biologists. As with the previous edition this book will dispel this idea by providing a detailed and rational guide to obtaining crystals or proteins and nucleic acids for diffraction studies.

As a response to the climate crisis and its effect on marine ecosystems and coastal populations, this book proposes concrete science driven solutions at establishing transformation pathways towards Sustainable Blue Growth, that are supported by technically and socially innovative innovations. This book proposes investment options and management solutions that have the potential of making our seas and oceans resilient to crises- climate, financial, health- by laying the foundations for a green/blue, circular economy that is anchored in science driven solutions and geared toward public well-being. Now is the time to usher in systemic economic change and the good news is that we have our blueprint: it's the combination of UN Agenda 2030 (17 SDG) and European Commission's European Green Deal! There is no doubt that the Earth's survival will depend on the protection and sustainable management of our seas and oceans and the resources they provide. This is recognized by the Joint Communication on International Ocean Governance, which is an integral part of the EU's response to the United Nations' 2030 Agenda for Sustainable Development, and in particular to the targets set out by Sustainable Development Goal 14 (SDG 14) to "conserve and sustainably use the oceans, seas and marine resources". The analytical framework and science-driven concrete management solutions proposed in this book can accelerate the transition to a sustainable management of our seas and oceans, by turning the current challenges into opportunities for sustainable economic growth which is both environmentally resilient and leaves no one behind.

The main aim of this book is to provide a broad overview of nuclear physics in terms of both hadron-meson dynamics and quark-lepton dynamics. It covers topics such as elastic and inelastic scattering, spin-isospin responses and charge exchange reactions, giant resonances, nuclear clusters, and nuclear physics with strange flavour. All subjects are presented from an experimental point of view, and sufficient prerequisite material is included for the book to be accessible to graduate students. An important feature is a discussion of the prevailing questions that emerge from recent research.

Related Title: Laboratory Scientific Glassblowing: Advanced Techniques and Glassblowing's Place in History'If you are interested in learning about glassblowing techniques for scientific glassware, then this book is an incredible opportunity to learn from a master glassblower. Much of this information is passed down in person, and to have it available in a book such as this is a very rare opportunity that you should not pass up.'IEEE Electrical Insulation MagazineThis book explains and demonstrates the methods involved in scientific glassblowing. It describes elementary to advanced glass manipulation together with technical information on its safe use and development in the laboratory. Edited by Paul Le Pinnet (MBE), a scientific glassblower with over 50 years' experience in the field, experts in glassblowing are brought together to explain their methods and approaches used to produce a variety of glassware.Laboratory Scientific Glassblowing is a unique project which updates and develops the traditional art of glassblowing and brings it into the 21st century. New skills and materials are introduced, including descriptions of working with fused silica, on laser profile cutting and on the creation of artistic glassware in a scientific setting. Written specifically as a hands-on reference work, this book can be used as a step-by-step practical guide for practitioners and scientists as well as students and apprentices interested in the field.Contributions from: Michael Baumbach, MD of H Baumbach & Co; Paul Rathmill, Enterprise Q; William Fludgate, MD BioChem Glass (app) Ltd; Ian Pearson (Past Chairman BSSG), Editor, BSSG Journal; Gary Coyne, California State University USA; Konstantin Kraft-Poggensee, Former chairman, German Scientific Glassblowing Society; Keith Holden President of the Australian and New Zealand Glassblowing Society; Phil Murray, Churchill Fellow.

Superconducting devices, which can carry huge currents and generate strong magnetic fields without losing energy, are improving at a tremendous pace. This book provides a modern, up-to-date reference on both the physics and the technology of superconducting magnets. It is unique in combining the theoretical aspects of superconductivity, electromagnetic field theory, and the thermodynamics of helium cooling with the technological details of producing and engineering high performance superconducting materials. The book provides the reliable, expert advice for designing, manufacturing, and testing complex high field superconducting magnets of predictable performance, and it places particular emphasis on beam transport and accelerator magnets in high energy particle physics.

The interaction of light with matter, in particular metals, is one of the classical areas of physical studies, and has contributed tremendously to our present understanding of physics. Light has been used successfully to investigate the electronic, magnetic and atomic structure of metal surfaces, as well as thin films, multi-layers, and interfaces. Such optical studies represent a non-destructive technique for materials characterization. The study of magnetism is of particular interest, not only for basic research, but also in view of a variety of applications like storage of information and magnetic recording. For many years the linear Kerr effect, typically exhibiting in metals Kerr rotations of less than one degree, has been used and developed as a successful tool for solid state physics research and applications. Only recently nonlinear optical effects in metals and in particular nonlinear magneto-optical effects have become an intensive area of studies. Due to the high interface sensitivity of nonlinear magneto-optics in contrast to linear magneto-optics, such studies lead to a new tool of investigating electronic stucture and magnetism at metallic interfaces, in thin films and multilayers. The high sensitivity of nonlinear optics and in particular the related, strikingly large Kerr rotations have been a remarkable experimental observation and an impressive example that Maxwell's equations still offer surprises. While future work on electronic and atomic structural phase transitions, on lateral and in-depth resolution of film structure, magnetic contrasts, domain structures, anti-ferromagnetism, or magnetic anisotropy effects will reveal the full potential of second harmonic light generation as a new tool of interface and film research, this book will give a comprehensive introduction to the state of the art in the subject, and will lay the ground for further developments.

This volume, like those prior to it, features chapters by experts in various fields of computational chemistry. Volume 19 is centered on the theme of macroscopic modeling, and discusses topics such as: Monte Carlo simulation techniques, computing hydrophobicity, classical trajectory simulations within the Born-Oppenheimer approximation, and the theory behind the widely used Poisson-Boltzmann equation.

FROM REVIEWS OF THE SERIES

"Reviews in Computational Chemistry remains the most valuable reference to methods and techniques in computational chemistry."
–JOURNAL OF MOLECULAR GRAPHICS AND MODELLING

"One cannot generally do better than to try to find an appropriate article in the highly successful Reviews in Computational Chemistry. The basic philosophy of the editors seems to be to help the authors produce chapters that are complete, accurate, clear, and accessible to experimentalists (in particular) and other nonspecialists (in general)."
–JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Includes recent research and development in the areas of omics and microbial bioremediation Covers the broad environmental pollution control approach such as metagenomics, metabolomics, fluxomics, bioremediation, and biodegradation of industrial wastes Reviews metagenomics and waste management, and recycling for environmental cleanup Describes the metagenomic methodologies and best-practices, from sample collection to data analysis for taxonomies Explores various microbial degradation pathways and detoxification mechanisms for organic and inorganic contaminants of wastewater with their gene expression

A zebrafish, the hull of a miniature ship, a mathematical equation and a food chain - what do these things have in common? They are examples of models used by scientists to isolate and study particular aspects of the world around us. This book begins by introducing the concept of a scientific model from an intuitive perspective, drawing parallels to mental models and artistic representations. It then recounts the history of modelling from the 16th century up until the present day. The iterative process of model building is described and discussed in the context of complex models with high predictive accuracy versus simpler models that provide more of a conceptual understanding. To illustrate the diversity of opinions within the scientific community, we also present the results of an interview study, in which ten scientists from different disciplines describe their views on modelling and how models feature in their work. Lastly, it includes a number of worked examples that span different modelling approaches and techniques. It provides a comprehensive introduction to scientific models and shows how models are constructed and used in modern science. It also addresses the approach to, and the culture surrounding modelling in different scientific disciplines. It serves as an inspiration for model building and also facilitates interdisciplinary collaborations by showing how models are used in different scientific fields. The book is aimed primarily at students in the sciences and engineering, as well as students at teacher training colleges but will also appeal to interested readers wanting to get an overview of scientific modelling in general and different modelling approaches in particular.

This book provides a definitive account of the theory, practice and applications of atom probe field ion microscopy (APFIM). The APFIM technique provides a unique method for observing and chemically identifying single atoms on solid surfaces. Recent advances in the method,which are largely due to the present authors, now permit the atomic-scale chemistry of a solid specimen to be recognised in three dimensions. As a result of these developments, new and exciting applications are rapidly emerging in the field of material science, surface science, and catalysis. The book is a state-of-the art account of this important field, and is intended for a graduate-level readership.

* Inclusion of realistic 3D simulations that behave very much like the real thing. This isn't just setting a value and reading something off the screen. These incorporate the physicality of the experiments, which might mean positioning yourself so that a moving needle can be seen accurately by using your position to remove parallax. * Based on academic research into teaching online. * Coverage of all of the required practicals (AQA). * Inclusion of background information on each experiment. * Detailed accounts of how to perform the experiment for real or with the simulation. * The simulations have the Association for Science's Green Tick of approval.