Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics. Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches.

A daily glass of wine prolongs life-yet alcohol can cause life-threatening cancer. Some say raising the minimum wage will decrease inequality while others say it increases unemployment. Scientists once confidently claimed that hormone replacement therapy reduced the risk of heart disease but now they equally confidently claim it raises that risk. What should we make of this endless barrage of conflicting claims? Observation and Experiment is an introduction to causal inference by one of the field's leading scholars. An award-winning professor at Wharton, Paul Rosenbaum explains key concepts and methods through lively examples that make abstract principles accessible. He draws his examples from clinical medicine, economics, public health, epidemiology, clinical psychology, and psychiatry to explain how randomized control trials are conceived and designed, how they differ from observational studies, and what techniques are available to mitigate their bias. "Carefully and precisely written...reflecting superb statistical understanding, all communicated with the skill of a master teacher." -Stephen M. Stigler, author of The Seven Pillars of Statistical Wisdom "An excellent introduction...Well-written and thoughtful...from one of causal inference's noted experts." -Journal of the American Statistical Association "Rosenbaum is a gifted expositor...an outstanding introduction to the topic for anyone who is interested in understanding the basic ideas and approaches to causal inference." -Psychometrika "A very valuable contribution...Highly recommended." -International Statistical Review

The new edition of this widely-used sourcebook details the startlingly array of diagnostic equipment available in the medical laboratory of the nineties, and also covers maintenance and quality assurance for each type of instrument. This book includes 17 completely rewritten chapters and 7 new ones, on nephelometry and turbidimetry, gas chromatography, mass spectrometry, flow cytometry, automated immunoassay systems, automated blood bank systems, and physician's office laboratory instrumentation.

Spark scientific curiosity from a young age with this six-level course through an enquiry-based approach and active learning. Collins International Primary Science fully meets the requirements of the Cambridge Primary Science Curriculum Framework from 2020 and has been carefully developed for a range of international contexts. The course is organised into four main strands: Biology, Chemistry, Physics and Earth and Space and the skills detailed under the 'Thinking and Working Scientifically' strand are introduced and taught in the context of those areas. For each Teacher's Guide at Stages 1 to 6, we offer: A comprehensive Teacher's Guide is easy to follow with a clear and consistent lesson plan layout, including built in continuous assessment The Teacher's Guide Plus ebook includes components such as slideshows, video clips, additional photographs and interactive activities Earth and Space content covers the new curriculum framework Thinking and Working Scientifically deepens and enhances the delivery of Science skills Includes practical activities that don't require specialist equipment or labs Scaffolding allows students of varying abilities to work with common content and meet learning objectives Supports Cambridge Global Perspectives (TM) with activities that develop and practise key skills Provides teacher support as part of a set of resources for the Cambridge Primary Science curriculum framework (0097) from 2020 This series is endorsed by Cambridge Assessment International Education to support the new curriculum framework 0097 from 2020.

Designed to enable newcomers to electrophysiology to choose appropriate preparations and techniques for in vitro studies in vertebrate neurobiology. Emphasizes techniques involving the glass micropipette and other recently developed methods, supplementing easy-to-follow instructions with illustrative schemes, examples of original records, photomicrographs, key word listings, comprehensive reference tables and a list of suppliers and key equipment and reagents.

This text is a compilation of practical information on the analysis of mineral type materials and products by the fused, cast bead method. It discusses the necessary equipment and its use; the processes involved in loss of ignition and decomposition by fusion and their chemistry. The book is also concerned with spectrometric parameters and the analytical procedures for a wide range of materials which, together with tables in the appendices, aim to allow easy reference to the parameters to be adopted to follow a defined procedure for most types of sample. The overall aim of the work is to help towards standardizing procedures, equipment and software, which should help to reduce costs and make for a better inter-laboratory comparison of results.

Fills the need for an experimental physics text. There are three main sections of the text. The first is an introduction that offers valuable insights into the importance of the human element in physics and traces the course of its historical development. This section also explains the objectives of the physics laboratory and the skills you must master to maintain a ``Notebook'' and analyze data, and presents a general discussion of spectroscopy experiments. The second section discusses the unique and valuable role of the computer in the laboratory and explains how to use it; software is included with the text. The final section contains over twenty experiments, providing students with a broad introduction into the use of a variety of instruments for carrying out many different measurements.

This book offers a beginner's guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyone with a general interest in these valuable tools.

Aliphatic and Alicyclic Compounds.

Monocyclic Aromatic Compounds.

Polycyclic Aromatic Compounds and Their Reduction Products.

Steroids.

Five-Membered Ring Heterocycles.

Six-Membered Ring Heterocycles.

Five-Membered Ring Benzofused Heterocycles.

Six-Membered Ring Benzofused Heterocycles.

Bicyclic Fused Heterocycles.

β-Lactam Antibiotics.

Miscellaneous Heterocycles.

Indexes.

What can you learn about your world in just a moment? Have you ever wondered why the sky is blue? Or whether dogs can read our facial expressions? Don Glass and experts in their fields answer these questions and many more. Written for readers of all ages with no background in science required, How the World Looks to a Bee is the perfect armchair companion for curious people who want to know more about the science of everyday life but have only a moment to spare. With intriguing everyday phenomena as a starting point, this entertaining collection uses short tutorials and quick and simple experiments to invite readers to test the science for themselves. These fascinating and topical science stories are sure to delight the curious child in all of us.

Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.

This book is a special edition, compiled for to the MSc Course Research Methodologies as taught at the Faculty of Aerospace Engineering at Delft University of Technology. It is a compilation of useful chapters from several sources on how to structure, set up, carry out and write up your (thesis) research to aid you in writing your research plan. Next to that it acts as a companion during your thesis research. After introducing you to the philosophy of scientific research, subsequent chapters each contribute to the different phases of your research. The book uniquely allows for the often multi- or interdisciplinary research many of you carry out, based on the established Dutch university tradition of (semi-)independent student research, creating a thread through the process for you to follow. This edition is a collection of chapters from An Introduction to Interdisciplinary Research (2016), edited by Steph Menken and Machiel Keestra, and Academic Skills for Interdisciplinary Studies. Revised edition (2019), by Koen van der Gaast, Laura Koenders and Ger Post, published by Amsterdam University Press.

"Fluorescence Microscopy: Super-Resolution and other Novel Techniques" delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers.

With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, "Fluorescence Microscopy" encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research.
Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical researchOffers insight into common challenges in implementing techniques, as well as effective solutions Provides video accompaniment for certain techniques on a companion website for a multi-dimensional perspective: http: //booksite.elsevier.com/9780124095137

The go-to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope--giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy--specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe's theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.
* Expands coverage to include discussion of confocal microscopy not found in the previous edition
* Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

The Beginnings of Electron Microscopy - Part 2, Volume 221 in the Advances in Imaging and Electron Physics series, highlights new advances in the field, with this new volume presenting interesting chapters on Recollections from the Early Years: Canada-USA, My Recollection of the Early History of Our Work on Electron Optics and the Electron Microscope, Walter Hoppe (1917-1986), Reminiscences of the Development of Electron Optics and Electron Microscope Instrumentation in Japan, Early Electron Microscopy in The Netherlands, L. L. Marton, 1901-1979, The Invention of the Electron Fresnel Interference Biprism, The Development of the Scanning Electron Microscope, and much more.

A detailed presentation of the physics of electron beam-specimen interactions Electron microscopy is one of the most widely used characterisation techniques in materials science, physics, chemistry, and the life sciences. This book examines the interactions between the electron beam and the specimen, the fundamental starting point for all electron microscopy. Detailed explanations are provided to help reinforce understanding, and new topics at the forefront of current research are presented. It provides readers with a deeper knowledge of the subject, particularly if they intend to simulate electron beam-specimen interactions as part of their research projects. The book covers the vast majority of commonly used electron microscopy techniques. Some of the more advanced topics (annular bright field and dopant atom imaging, atomic resolution chemical analysis, band gap measurements) provide additional value, especially for readers who have access to advanced instrumentation, such as aberration-corrected and monochromated microscopes. Electron Beam-Specimen Interactions and Simulation Methods in Microscopy offers enlightening coverage of: the Monte-Carlo Method; Multislice Simulations; Bloch Waves in Conventional and Analytical Transmission Electron Microscopy; Bloch Waves in Scanning Transmission Electron Microscopy; Low Energy Loss and Core Loss EELS. It also supplements each chapter with clear diagrams and provides appendices at the end of the book to assist with the pre-requisites. A detailed presentation of the physics of electron beam-specimen interactions Each chapter first discusses the background physics before moving onto simulation methods Uses computer programs to simulate electron beam-specimen interactions (presented in the form of case studies) Includes hot topics brought to light due to advances in instrumentation (particularly aberration-corrected and monochromated microscopes) Electron Beam-Specimen Interactions and Simulation Methods in Microscopy benefits students undertaking higher education degrees, practicing electron microscopists who wish to learn more about their subject, and researchers who wish to obtain a deeper understanding of the subject matter for their own work.

Electron cryomicroscopy is a form of transmission electron microscopy (EM) in which the sample is studied at cryogenic temperatures (generally liquid nitrogen temperatures). Cryo-EM is developing popularity in structural biology. This volume from the Advances in Protein Chemistry and Structural Biology series is Part B and covers essential topics.

The Welfare of Animals used in Research: Practice and Ethics gives a complete and balanced overview of the issues surrounding the use of animals in scientific research. The focus of the book is on the animal welfare implications and ethics of animals in research. It covers the topics with sufficient depth to show a real understanding of varied and complex subjects, but conveys the information in a beautifully reader-friendly manner. Key features: * Provides those who are not working in the field with a reasonable understanding as to why and how animals are used in research. * Gives an introduction to the ethical issues involved in using animals, and explains how these are addressed in practice. * Details the advances in animal welfare and the use and development of the 3Rs principles, and how these have become fundamental to the everyday use and regulation of animals used in research. * The focus is on principles making it suitable for an international audience. This book is a useful introduction to the issues involved in laboratory animal welfare for those who intend to work in research involving animals. It is also useful to prospective animal care staff and animal welfare scientists, and to those involved in ethical review. It will help inform debate amongst those who are not involved in experimentation but who are interested in the issues. Published as a part of the prestigious Wiley-Blackwell UFAW Animal Welfare series. UFAW, founded 1926, is an internationally recognised, independent, scientific and educational animal welfare charity. For full details of all titles available in the series, please visit the

What can you learn about your world in just a moment? Have you ever wondered why the sky is blue? Or whether dogs can read our facial expressions? Don Glass and experts in their fields answer these questions and many more. Written for readers of all ages with no background in science required, How the World Looks to a Bee is the perfect armchair companion for curious people who want to know more about the science of everyday life but have only a moment to spare. With intriguing everyday phenomena as a starting point, this entertaining collection uses short tutorials and quick and simple experiments to invite readers to test the science for themselves. These fascinating and topical science stories are sure to delight the curious child in all of us.

As part of the Reliable Lab Solutions series, Techniques in Confocal Microscopy brings together chapters from volumes 302, 307 and 356 of Methods in Enzymology. It documents many diverse uses for confocal microscopy in disciplines that broadly span biology.

It documents many diverse uses for confocal microscopy in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in the initial publications Techniques are described in a context that allows comparisons to other related methodologies. Methodologies are laid out in a manner that stresses their general applicability and reports their potential limitations.

Structural genomics is the systematic determination of 3-D structures of proteins representative of the range of protein structure and function found in nature. The goal is to build a body of structural information that will predict the structure and potential function for almost any protein from knowledge of its coding sequence. This is essential information for understanding the functioning of the human proteome, the ensemble of tens of thousands of proteins specified by the human genome.
While most structural biologists pursue structures of individual proteins or protein groups, specialists in structural genomics pursue structures of proteins on a genome wide scale. This implies large-scale cloning, expression and purification. One main advantage of this approach is economy of scale.
Key Features
*Examines the three dimensional structure of all proteins of a given organism, by experimental methods such as X-ray crystallography and NMR spectroscopy
* Looks at structural genomics as a foundation of drug discovery as discovering new medicines is becoming more challenging and the pharmaceutical industry is looking to new technologies to help in this mission

The accurate measurement of temperature is a vital parameter in many fields of engineering and scientific practice. Responding to emerging trends, this classic reference has been fully revised to include coverage of the latest instrumentation and measurement methods.

Featuring:

• Brand new chapters on computerised temperature measuring systems and temperature measurement in medicine.

• Sections on noise thermometers, the development of photoelectric and two-wave pyrometers and the latest IEC (International Electrotechnical Commission) standards

• Coverage of fibre optic thermometers, imaging of temperature fields and measurement in hazardous areas.

• Examination of virtual instruments, fuzzy logic in temperature measurement and new methods for thermometer calibration.

Practising instrument engineers, graduate students and researchers in the fields of mechanical, electrical and electronic engineering will welcome this balanced approach to both the theory and practice of temperature measurement.

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

* Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide

This volume, along with Part A and Part B, is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

* Includestime-tested coremethods and new innovationsapplicable to any researcher * Methods included are useful to both establishedresearchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide"