The book describes recent progress of near-field optical science and technology. The title of the book implies capabilities of optical near-field not only for imaging/microscopy but also for fabrication/manipulation/processing in nanometric scale. The authors introduce the differences between near-field optics and far-field optics from both an experimental and theoretical perspective. The book touches on a wide range of topics in near-field optics, and can be used both by the novice and experienced researcher already familiar with the subject, to connect the experimental with the theoretical aspects of near-field optics.

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation

THIS VOLUME, WHICH IS DESIGNED FOR STAND-ALONE USE IN TEACHING AND RESEARCH, FOCUSES ON QUANTUM CHEMISTRY, AN AREA OF SCIENCE THAT MANY CONSIDER TO BE THE CENTRAL CORE OF COMPUTATIONAL CHEMISTRY. TUTORIALS AND REVIEWS COVER
* HOW TO OBTAIN SIMPLE CHEMICAL INSIGHT AND CONCEPTS FROM DENSITY FUNCTIONAL THEORY CALCULATIONS,
* HOW TO MODEL PHOTOCHEMICAL REACTIONS AND EXCITED STATES, AND
* HOW TO COMPUTE ENTHALPIES OF FORMATION OF MOLECULES.
* A FOURTH CHAPTER TRACES CANADIAN RESEARCH IN THE EVOLUTION OF COMPUTATIONAL CHEMISTRY.
* ALSO INCLUDED WITH THIS VOLUME IS A SPECIAL TRIBUTE TO QCPE. FROM REVIEWS OF THE SERIES
"Reviews in Computational Chemistry proves itself an invaluable resource to the computational chemist. This series has a place in every computational chemist's library."-JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression.
Key Features
* Provides well-tested experimental protocols for each technique
* Lists the reagents and preparation of each experiment separately
* Contains a complete schedule of experiments and the preparation required
* Includes study questions at the end of each chapter

For many years, evidence suggested that all solid materials either possessed a periodic crystal structure as proposed by the Braggs or they were amorphous glasses with no long-range order. In the 1970s, Roger Penrose hypothesized structures (Penrose tilings) with long-range order which were not periodic. The existence of a solid phase, known as a quasicrystal, that possessed the structure of a three dimensional Penrose tiling, was demonstrated experimentally in 1984 by Dan Shechtman and colleagues. Shechtman received the 2011 Nobel Prize in Chemistry for his discovery. The discovery and description of quasicrystalline materials provided the first concrete evidence that traditional crystals could be viewed as a subset of a more general category of ordered materials. This book introduces the diversity of structures that are now known to exist in solids through a consideration of quasicrystals (Part I) and the various structures of elemental carbon (Part II) and through an analysis of their relationship to conventional crystal structures. Both quasicrystals and the various allotropes of carbon are excellent examples of how our understanding of the microstructure of solids has progressed over the years beyond the concepts of traditional crystallography.

This book provides a comprehensive introduction to the methods and variety of Kelvin probe force microscopy, including technical details. It also offers an overview of the recent developments and numerous applications, ranging from semiconductor materials, nanostructures and devices to sub-molecular and atomic scale electrostatics. In the last 25 years, Kelvin probe force microscopy has developed from a specialized technique applied by a few scanning probe microscopy experts into a tool used by numerous research and development groups around the globe. This sequel to the editors' previous volume "Kelvin Probe Force Microscopy: Measuring and Compensating Electrostatic Forces," presents new and complementary topics. It is intended for a broad readership, from undergraduate students to lab technicians and scanning probe microscopy experts who are new to the field.

With respect to chemical applications, surface-launched acoustic wave sensors were originally developed as sensing devices for specific chemical and biological species, but more recently have been applied to the study of thin film and interfacial properties. These devices exploit the phenomenon of piezoelectricity, the instigation of mechanical motion in solids by oscillating electrical fields. This text/reference presents the principles of design and operation of these sensors and explores their traditional and emerging applications with a focus on devices that employ acoustic waves launched and received on the same surface.

The book begins with a review of piezoelectricity and the genesis of acoustic wave devices, and the advent of chemical sensor technology. Subsequent chapters explore acoustic waves in solids and device structure, theory of acoustic wave response, and the various categories of acoustic wave device. The book describes the design of these devices and how they are applied in chemistry for the detection of species present in the gas and liquid phase, as well as the study of thin films placed on the sensor surface. Other topics covered include polymeric glass transitions, polymer properties, biosensor technology, and the development of sensor arrays. Each of the various types of device is examined with a view toward its application in chemistry in general and analytical chemistry in particular.

Presenting the most up-to-date information available on this rapidly evolving technology, and supplemented with scores of helpful illustrations and tables, Surface-Launched Acoustic Wave Sensors draws information from such diverse areas of scientific investigation as acoustic wave physics, applied mathematics, chemistry, electronics, fluid mechanics, materials science, piezoelectricity, and polymer science. The material presented on these topics is both self-consistent and readable for the nonexpert—allowing industrial chemists, graduate students, and undergraduates to gain a deeper understanding of these devices, their designs, and applications.

A focused and accessible presentation of a burgeoning new technology

This book concerns the design, operation, and application of devices capable of generating acoustic waves in the ultrasonic frequency range. The clear emphasis of the text is the study of chemical and/or biochemical systems imposed on the surface of such devices, whether operated in the gas or liquid phase, i.e., on acoustic wave chemical and biological sensors. Presenting the most up-to-date information available on this rapidly evolving technology, and supplemented with scores of helpful illustrations and tables, this book

• Reviews piezoelectricity and the genesis of acoustic wave devices as well as the advent of chemical sensor technology
• Explores acoustic waves in solids and device structure, theory of acoustic wave response, and the various categories of acoustic wave device
• Describes device design and how these devices are applied in chemistry to detect species present in the gas and liquid phase, as well as to study thin films placed on the sensor surface
• Covers polymeric glass transitions, polymer properties, biosensor technology, and the development of sensor arrays
• Examines each of the various types of device with a view toward its application in analytical chemistry and chemistry in general

The participation in interlaboratory studies and the use of Certified Reference Materials (CRMs) are widely recognised tools for the verification of the accuracy of analytical measurements and they form an integral part of quality control systems used by many laboratories, e.g. in accreditation schemes. As a response to the need to improve the quality of environmental analysis, the European Commission has been active in the past fifteen years, through BCR activity (now renamed Standards, Measurements and Testing Programme) in the organisation of series of interlaboratory studies involving expert laboratories in various analytical fields (inorganic, trace organic and speciation analysis applied to a wide variety of environmental matrices). The BCR and its successor have the task of helping European laboratories to improve the quality of measurements in analytical sectors which are vital for the European Union (biomedical, agriculture, food, environment and industry); these are most often carried out in support of EC regulations, industrial needs, trade, monitoring activities (including environment, agriculture, health and safety) and, more generally, when technical difficulties hamper a good comparability of data among EC laboratories. The collaborative projects carried out so far have placed the BCR in the position of second world CRM producer (after NIST in the USA).

"Interlaboratory Studies and Certification of Reference Materials for Environmental Analysis" gives an account of the importance of reference materials for the quality control of environmental analysis and describes in detail the procedures followed by BCR to prepare environmental reference materials, including aspects related to sampling, stabilization, homogenisation, homogeneity and stability testing, establishment of reference (or certified) values, and use of reference materials. Examples of environmental CRMs produced by BCR within the last 15 years are given, which represent more than 70 CRMs covering different types of materials (plants, biological materials, waters, sediments, soils and sludges, coals, ash and dust materials) certified for a range of chemical parameters (major and trace elements, chemical species, PAHs, PCBs, pesticides and dioxins).

The final section of the book describes how to organise improvement schemes for the evaluation method and/or laboratory performance. Examples of interlaboratory studies (learning scheme, proficiency testing and intercomparison in support to prenormative research) are also given.

This book focuses on the morphology, exine ornamentation and the associated evolutionary trends of crabapple pollen and anatomical developmental patterns. To examine the genetic evolutionary patterns of crabapple pollen traits, we constructed an interval distribution function based on characteristic pollen parameters and used a binary trivariate data matrix (Xi Yi Zi) to reflect the exine ornamentation regularity of the pollen. Our findings should inform the taxonomic status of the genus Malus. Pollen electron micrographs from a total of 26 species and 81 cultivars of Malus were recorded in this book. All 107 figures and 642 scanned pollen images constitute primary data obtained by the authors. The images in this book are clear, three-dimensional, and aesthetically pleasing. They are accompanied with text descriptions and provided a method for the indication of the different types of information that can be expected. This book can provide a reference for scientific researchers, students, and teachers in tertiary institutions that are engaged in research concerning crabapple production.

Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics. Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches.

Silicon, the basic material for a multibillion-dollar industry, is the most widely researched and applied semiconductor, and its surfaces are the most thoroughly studied of all semiconductor surfaces. Silicon Surfaces and Formation of Interfaces may be used as an introduction to graduate-level physics and chemical physics. Moreover, it gives a specialized and comprehensive description of the most common faces of silicon crystals as well as their interaction with adsorbates and overlayers. This knowledge is presented in a systematic and easy-to-follow way. Discussion of each system is preceded by a brief overview which categorizes the features and physical mechanisms before the details are presented. The literature is easily available, and the references am numerous and organized in tables, allowing a search without the need to browse through the text.

Though this volume focuses on a scientific understanding of physics on the atomistic and mesoscopic levels, it also highlights existing and potential links between basic research in surface science and applications in the silicon industry. It will be valuable to anyone writing a paper, thesis, or proposal in the field of silicon surfaces.

This issue of Dermatologic Clinics, guest edited by Jane M. Grant-Kels, Giovanni Pellacani, and Caterina Longo, is devoted to Confocal Microscopy. Articles in this timely issue include: Basics of Confocal Microscopy and the Complexity of Diagnosing Skin Tumors: New Imaging Tools in Clinical Practice, Diagnostic Workflows, Cost-estimate and New Trends; Opening a Window Into Living Tissue: Histopathologic Features of Confocal Microscopic Findings in Skin Tumors; Addressing the Issue of Discriminating Nevi from Early Melanomas: Dues and Pitfalls; Melanoma Types and Melanoma Progression: The Different Faces; Lentigo Maligna, Macules of the Face and Lesions on Sun-damaged Skin: Confocal makes the Difference; Glowing in the dark: use of confocal microscopy in dark pigmented lesions; Enlightening the Pink: Use of Confocal Microscopy in Pink Lesions; Shining into the White: The Spectrum of Epithelial Tumors from Actinic Keratosis to SCC; Application of Wide-probe and Handy-probe for Skin Cancer Diagnosis: Pros and Cons; Confocal Microscopy for Special Sites and Special Uses; Confocal Algorithms for Inflammatory Skin Diseases and Hair Diseases; In Vivo and Ex Vivo Confocal Microscopy for Dermatologic and Mohs' Surgeons; Telediagnosis with Confocal Microscopy: A Reality or a Dream?; "Well-aging": Early Detection of Skin Aging Signs; The Role of Confocal Microscopy in Clinical Trials for Treatment Monitoring; and Fluorescence (multiwave) Confocal Microscopy.

cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging.

Provides extensive and thoroughly exhaustive coverage of precision laser spectroscopy Presents chapters written by recognized experts in their individual fields Topics covered include cold atoms, cold molecules, methods and techniques for production of cold molecules, optical frequency standards based on trapped single ions, etc Applicable for researchers and graduate students of optical physics and precision laser spectroscopy

This book covers the fundamentals of Helium Ion Microscopy (HIM) including the Gas Field Ion Source (GFIS), column and contrast formation. It also provides first hand information on nanofabrication and high resolution imaging. Relevant theoretical models and the existing simulation approaches are discussed in an extra section. The structure of the book allows the novice to get acquainted with the specifics of the technique needed to understand the more applied chapters in the second half of the volume. The expert reader will find a complete reference of the technique covering all important applications in several chapters written by the leading experts in the field. This includes imaging of biological samples, resist and precursor based nanofabrication, applications in semiconductor industry, using Helium as well as Neon and many more. The fundamental part allows the regular HIM user to deepen his understanding of the method. A final chapter by Bill Ward, one of the pioneers of HIM, covering the historical developments leading to the existing tool complements the content.

This book describes modern focused ion beam microscopes and techniques and how they can be used to aid materials metrology and as tools for the fabrication of devices that in turn are used in many other aspects of fundamental metrology. Beginning with a description of the currently available instruments including the new addition to the field of plasma-based sources, it then gives an overview of ion solid interactions and how the different types of instrument can be applied. Chapters then describe how these machines can be applied to the field of materials science and device fabrication giving examples of recent and current activity in both these areas.

Introducing students to basic lab techniques and illustrating core chemical principles Prepared by John H. Nelson and Kenneth C. Kemp, both of the University of Nevada, this manual contains 43 finely tuned experiments chosen to introduce students to basic lab techniques and to illustrate core chemical principles. In the 14th Edition, all experiments were carefully edited for accuracy, safety, and cost. Pre-labs and questions were revised and new experiments added concerning solutions, polymers, and hydrates. Each of the experiments is self-contained, with sufficient background material, enabling students to conduct and understand the experiment. Each has a pedagogical objective to exemplify one or more specific principles. Because the experiments are self-contained, they may be undertaken in any order, although the authors have found in their General Chemistry course that the sequence of Experiments 1 through 7 provides the firmest background and introduction. To assist the student, the authors have included pre-lab questions for the student to answer before starting the lab. The questions are designed to help the student understand the experiment, to learn how to do the necessary calculations to treat their data, and as an incentive to read the experiment in advance.

This volume is a compilation of laboratory protocols and methodology required for the study of molecular chaperones and the cellular stress response. Chapters detail stress response in Hsf1, Hsf2 and Hsf4 knockout mice, mapping HSP interaction networks, the LUminescence-based Mammalian IntERactome (LUMIER), Hsp70 biology, protein folding activity of Hsp90, cytotoxicity of HSP inhibitors, computational approaches for modeling allosteric Hsp90 interactions, HSPs in immunity and vaccine development , and biologies of Hsp70 and Hsp90. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Chaperones: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Originally published in 1665, Micrographia is the most famous and influential work of English scholar ROBERT HOOKE (1635-1703), a notable member of the Royal Society and the scientist for whom Hooke's Law of elasticity is named. Here, Hooke describes his observations of various household and biological specimens, such as the eye of a fly and the structure of plants, and became the first person to use the term cell in biology, as the cells in plants reminded him of monk's living quarters. In addition to his studies using a microscope, Hooke also discusses the heavenly bodies as visible through a telescope. Students of science and the history of science will find Hooke's early forays into biology and optics a good primer for further learning.

The damage that can occur in certain fibrous raw materials or in textiles during their production and storage of textiles is expertly described in this book by Karl Mahall. In particular, he explains methods for finding concealed textile defects by using microscopic analysis.Besides minor improvements and corrections, the new edition contains a new chapter "Poultry Feathers as Filling Material for Bedding and Textiles - Analysis of Faults." The reason for its inclusion is that natural feathers and down are not only used as a filling material for bedding but also for garments, such as anoraks, coats and sleeping bags.This book is especially useful as a manual for both chemical and textile engineers and quality engineers. It is also a useful reference for others in the textile industry in general.

Unifies the complex welter of techniques used for chemical separations by clearly formulating the concepts that are common to them. The mass transport phenomena underlying all separation processes are developed in a simple physical-mathematical form. The limitations and optimum performance of alternative separation techniques and the factors enhancing and limiting separation power can thus be described and explored. Generously illustrated and contains numerous exercises. Long awaited in the scientific community, it breaks new ground in understanding separation processes.

This book illustrates the practical workings of environmental transmission electron microscopy (ETEM) from history and instrument design through to solving practical problems. Aspects of instrument design, performance, and operating procedures are covered, together with common problems and pitfalls of the technique. Not only will a properly operated instrument and a carefully set up experiment provide new insight into your specimen, but the ability to observe the specimen in its natural habitat will be essential to meeting specific design criteria for the development of the next generation of materials. Over the past five decades, transmission electron microscopy (TEM) under environmental conditions relevant to a particular sample has been of increasing interest. Symposia dealing with the topic are now among the best attended at international microscopy conferences. Since typical operating modes for the ETEM require the sample be subjected to a harsh environment consisting of corrosive gases and high temperatures, the challenges of adapting and operating the instrument for observation under dynamic operating conditions are numerous. However, careful consideration of the interaction of the electrons with the gases and sample, as well as the gases with the microscope components, can lead to highly rewarding results. In Controlled Atmosphere Transmission Electron Microscopy, leading experts help you to perform successful experiments using the ETEM, and to interpret and understand the results.

Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. "Patch-Clamp Methods and Protocols, Second Edition," provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, "Patch-Clamp Methods and Protocols, Second Edition" will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.