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Books > Science & Mathematics > Science: general issues > Scientific equipment & techniques, laboratory equipment
This book introduces the fundamentals of biobanking and guides through the practical planning thereof, with a special focus on the situation in low- and middle-income countries. On the example of the setup of a Ukrainian biobank the book discusses the main steps and aspects of successful biorepository implementation and management. Topics covered include collection, storage and shipping of samples, establishment of an IT system, development of a sustainability plan, and project and risk management. Furthermore, the importance of the formation of international biobanking societies such as the Ukraine Association of Biobanks is highlighted, and their main objectives and tasks are discussed.The book addresses life science and business professionals as well as national authorities who are interested in biobanking in general and in setting up a biobank in particular.
Uses questions about hypothetical situations to introduce the process of thinking according to scientific method.
"Veterinary Laboratory Medicine" covers all aspects of basic clinical biochemistry and haematology, and includes test-by-test interpretation of laboratory results. Information is provided on sampling techniques, the selection and use of an external laboratory, as well as near-patient testing and the practice laboratory. Also included are step-by-step instructions for most commonly used point-of-care tests, a guide to the evaluation of instruments for in-practice use, and a detailed explanation of the principles of impedance counting and photometric analysis. The book will be ideal for practitioners who require a guide to laboratory work, and for veterinary students studying laboratory medicine and clinical pathology. The second edition has been fully updated to reflect advances in diagnostic techniques, and includes new chapters on diagnostic endocrinology and feline virus testing as well as a much expanded chapter on diagnostic profiling and pattern recognition.
Micro-organisms play a major role in the geochemistry of the planet, forming the basic stage in the food chain, and thus sustaining the existence of higher evolutionary life. The continuing interaction between these living organisms and the environment, combined with their exploitation by man are shaping the material world today. Over the last few years our understanding has increased considerably due to the development of new technology and the emergence of new paradigms which have enabled the microbiologist to view the microbial world, and its significance to life, with new eyes. Combining the basics of science with the most up-to-date new material, and incorporating high quality photographs and graphics, this book is valuable as both a textbook and reference guide for students and professionals.
This book covers one of the most neglected areas in environmental trace analysis, namely that of sample preparation. The text discusses all of the necessary steps for analysing a sample for both trace metals and organics, from the initial sampling protocols, through the range of possible sample preparation methods, and leading finally to the analysis and recording of data. Detailed information is provided on the specific methods to follow for preparing a sample for metal and organic analyses. The topics covered include the following:
The design and analysis of experiments is typically taught as part of a second level course in statistics. Many different types and levels of students will require this information in order to progress with their studies and research. This text is thus offered as an introduction to this wide ranging and important subject. It has the advantage of explaining in an accessible way the basic principles behind good experimental thinking, planning and action. The authors have used their experience in teaching related courses to separate out what seem to be the essential basic contents for everyone, and to combine with these some of the most useful additional topics in biological, industrial, medical, and environmental experimentation.
This text provides the veterinary practitioner with an explanation of the meaning of laboratory results (biochemical, haematological and urinary) in the diagnosis of disorders in small animals. It enables veterinary surgeons in small animal practice to interpret the results of those laboratory tests that are commonly performed in order to arrive at a diagnosis or prognosis. Research has shown that this is one skill in which many veterinarians believe themselves to be deficient and would welcolm guidance; this volume explains the possible meanings of the values that could be obtained. The book covers the areas of haematology, plasma/serum biochemistry and urinalysis. Advice is given on the influence of drugs, sample collection and handling, how to check the accuracy of results, how to assess the reliability of a laboratory and the additional tests that would be indicated to confirm a diagnosis together with normal reference ranges and conversion ranges.
How do scientists impact society in the twenty-first century? Many scientists are increasingly interested in the impact that their research will have on the public. Scientists likewise must answer the question above when applying for funding from government agencies, particularly as part of the 'Broader Impacts' criterion of proposals to the US National Science Foundation. This book equips scientists in all disciplines to do just that, by providing an overview of the origins, history, rationale, examples, and case studies of broader impacts, primarily drawn from the author's experiences over the past five decades. Beyond including theory and evidence, it serves as a 'how to' guide for best practices for scientists. Although this book primarily uses examples from the NSF, the themes and best practices are applicable to scientists and applications around the world where funding also requires impacts and activities that benefit society.
This manual covers the latest laboratory techniques, state-of-the-art instrumentation, laboratory safety, and quality assurance and quality control requirements. In addition to complete coverage of laboratory techniques, it also provides an introduction to the inorganic nonmetallic constituents in environmental samples, their chemistry, and their control by regulations and standards.
We may learn from our mistakes, but Deborah Mayo argues that, where
experimental knowledge is concerned, we haven't begun to learn
enough. "Error and the Growth of Experimental Knowledge" launches a
vigorous critique of the subjective Bayesian view of statistical
inference, and proposes Mayo's own error-statistical approach as a
more robust framework for the epistemology of experiment. Mayo
genuinely addresses the needs of researchers who work with
statistical analysis, and simultaneously engages the basic
philosophical problems of objectivity and rationality.
"Provides a lot of reading pleasure and many new insights." —Journal of Molecular Structure "This is the most entertaining, stimulating and useful book which can be thoroughly recommended to anyone with an interest in computer simulation." —Contemporary Physics "A very useful introduction . . . more interesting to read than the often dry equation-based texts." —Journal of the American Chemical Society Written especially for the novice, Molecular Dynamics Simulation demonstrates how molecular dynamics simulations work and how to perform them, focusing on how to devise a model for specific molecules and then how to simulate their movements using a computer. This book provides a collection of methods that until now have been scattered through the literature of the last 25 years. It reviews elements of sampling theory and discusses how modern notions of chaos and nonlinear dynamics explain the workings of molecular dynamics. Stresses easy-to-use molecules
A popular book in its first edition, The Food Chemistry Laboratory: A Manual for Experimental Foods, Dietetics, and Food Scientists, Second Edition continues to provide students with practical knowledge of the fundamentals of designing, executing, and reporting the results of a research project. Presenting experiments that can be completed, in many cases, without requiring extensive student laboratory facilities, the authors include new exercises in the areas of physical properties, lipids, proteins, and gelatin. Also new in this edition are a brief introduction to each laboratory exercise and a listing of materials needed, approximate time needed for completion, and possible complications and/or pitfalls.
Correlative Light and Electron Microscopy III, Volume 140, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics discussed in this new release include Millisecond time-resolved CLEM, Super resolution LM und SEM of high-pressure frozen C. elegans, Preservation fluorescence, super res CLEM, APEX in Tissue, Corrsight mit IBIDI flowthrough chamber, Correlative Light Atomic Force Electronic Microscopy (CLAFEM), Atmospheric EM CLEM, and High-precision correlation, amongst other topics. Chapters in this ongoing series deal with different approaches for analyzing the same specimen using more than one imaging technique. The strengths and application area of each is presented, with this volume exploring the aspects of sample preparation of diverse biological systems for different CLEM approaches.
A daily glass of wine prolongs life-yet alcohol can cause life-threatening cancer. Some say raising the minimum wage will decrease inequality while others say it increases unemployment. Scientists once confidently claimed that hormone replacement therapy reduced the risk of heart disease but now they equally confidently claim it raises that risk. What should we make of this endless barrage of conflicting claims? Observation and Experiment is an introduction to causal inference by one of the field's leading scholars. An award-winning professor at Wharton, Paul Rosenbaum explains key concepts and methods through lively examples that make abstract principles accessible. He draws his examples from clinical medicine, economics, public health, epidemiology, clinical psychology, and psychiatry to explain how randomized control trials are conceived and designed, how they differ from observational studies, and what techniques are available to mitigate their bias. "Carefully and precisely written...reflecting superb statistical understanding, all communicated with the skill of a master teacher." -Stephen M. Stigler, author of The Seven Pillars of Statistical Wisdom "An excellent introduction...Well-written and thoughtful...from one of causal inference's noted experts." -Journal of the American Statistical Association "Rosenbaum is a gifted expositor...an outstanding introduction to the topic for anyone who is interested in understanding the basic ideas and approaches to causal inference." -Psychometrika "A very valuable contribution...Highly recommended." -International Statistical Review
The new edition of this widely-used sourcebook details the startlingly array of diagnostic equipment available in the medical laboratory of the nineties, and also covers maintenance and quality assurance for each type of instrument. This book includes 17 completely rewritten chapters and 7 new ones, on nephelometry and turbidimetry, gas chromatography, mass spectrometry, flow cytometry, automated immunoassay systems, automated blood bank systems, and physician's office laboratory instrumentation.
Spark scientific curiosity from a young age with this six-level course through an enquiry-based approach and active learning. Collins International Primary Science fully meets the requirements of the Cambridge Primary Science Curriculum Framework from 2020 and has been carefully developed for a range of international contexts. The course is organised into four main strands: Biology, Chemistry, Physics and Earth and Space and the skills detailed under the 'Thinking and Working Scientifically' strand are introduced and taught in the context of those areas. For each Teacher's Guide at Stages 1 to 6, we offer: A comprehensive Teacher's Guide is easy to follow with a clear and consistent lesson plan layout, including built in continuous assessment The Teacher's Guide Plus ebook includes components such as slideshows, video clips, additional photographs and interactive activities Earth and Space content covers the new curriculum framework Thinking and Working Scientifically deepens and enhances the delivery of Science skills Includes practical activities that don't require specialist equipment or labs Scaffolding allows students of varying abilities to work with common content and meet learning objectives Supports Cambridge Global Perspectives (TM) with activities that develop and practise key skills Provides teacher support as part of a set of resources for the Cambridge Primary Science curriculum framework (0097) from 2020 This series is endorsed by Cambridge Assessment International Education to support the new curriculum framework 0097 from 2020.
Designed to enable newcomers to electrophysiology to choose appropriate preparations and techniques for in vitro studies in vertebrate neurobiology. Emphasizes techniques involving the glass micropipette and other recently developed methods, supplementing easy-to-follow instructions with illustrative schemes, examples of original records, photomicrographs, key word listings, comprehensive reference tables and a list of suppliers and key equipment and reagents.
This text is a compilation of practical information on the analysis of mineral type materials and products by the fused, cast bead method. It discusses the necessary equipment and its use; the processes involved in loss of ignition and decomposition by fusion and their chemistry. The book is also concerned with spectrometric parameters and the analytical procedures for a wide range of materials which, together with tables in the appendices, aim to allow easy reference to the parameters to be adopted to follow a defined procedure for most types of sample. The overall aim of the work is to help towards standardizing procedures, equipment and software, which should help to reduce costs and make for a better inter-laboratory comparison of results.
Fills the need for an experimental physics text. There are three main sections of the text. The first is an introduction that offers valuable insights into the importance of the human element in physics and traces the course of its historical development. This section also explains the objectives of the physics laboratory and the skills you must master to maintain a ``Notebook'' and analyze data, and presents a general discussion of spectroscopy experiments. The second section discusses the unique and valuable role of the computer in the laboratory and explains how to use it; software is included with the text. The final section contains over twenty experiments, providing students with a broad introduction into the use of a variety of instruments for carrying out many different measurements.
This book offers a beginner's guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyone with a general interest in these valuable tools.
Aliphatic and Alicyclic Compounds. Monocyclic Aromatic Compounds. Polycyclic Aromatic Compounds and Their Reduction Products. Steroids. Five-Membered Ring Heterocycles. Six-Membered Ring Heterocycles. Five-Membered Ring Benzofused Heterocycles. Six-Membered Ring Benzofused Heterocycles. Bicyclic Fused Heterocycles. β-Lactam Antibiotics. Miscellaneous Heterocycles. Indexes.
What can you learn about your world in just a moment? Have you ever wondered why the sky is blue? Or whether dogs can read our facial expressions? Don Glass and experts in their fields answer these questions and many more. Written for readers of all ages with no background in science required, How the World Looks to a Bee is the perfect armchair companion for curious people who want to know more about the science of everyday life but have only a moment to spare. With intriguing everyday phenomena as a starting point, this entertaining collection uses short tutorials and quick and simple experiments to invite readers to test the science for themselves. These fascinating and topical science stories are sure to delight the curious child in all of us.
Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.
"Fluorescence Microscopy: Super-Resolution and other Novel Techniques" delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers. With its extensive graphics, inter-method comparisons, and
tricks and approaches not revealed in primary publications,
"Fluorescence Microscopy" encourages readers to both understand
these methods, and to adapt them to other systems. It also offers
instruction on the best visualization to derive quantitative
information about cell biological structure and function,
delivering crucial guidance on best practices in related laboratory
research.
The go-to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope--giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy--specifically, those with a need to understand the application, limitations, and strengths of FEGSEM. |
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