* Inclusion of realistic 3D simulations that behave very much like the real thing. This isn't just setting a value and reading something off the screen. These incorporate the physicality of the experiments, which might mean positioning yourself so that a moving needle can be seen accurately by using your position to remove parallax. * Based on academic research into teaching online. * Coverage of all of the required practicals (AQA). * Inclusion of background information on each experiment. * Detailed accounts of how to perform the experiment for real or with the simulation. * The simulations have the Association for Science's Green Tick of approval.

The Transmission Electron Microscope (TEM) is the ultimate tool to see and measure structures on the nanoscale and to probe their elemental composition and electronic structure with sub-nanometer spatial resolution. Recent technological breakthroughs have revolutionized our understanding of materials via use of the TEM, and it promises to become a significant tool in understanding biological and biomolecular systems such as viruses and DNA molecules. This book is a practical guide for scientists who need to use the TEM as a tool to answer questions about physical and chemical phenomena on the nanoscale.

Details the use of advanced AFMs and addresses all types of functional AFMs First book to focus on application of AFM for energy research Enables readers to operate an AFM successfully and to understand the data obtained Covers new achievements in AFM instruments, including higher speed and resolution, automatic and deep learning AFM, and how AFM is being combined with other new methods like IR and Raman microscopy

* Inclusion of realistic 3D simulations that behave very much like the real thing. This isn't just setting a value and reading something off the screen. These incorporate the physicality of the experiments, which might mean positioning yourself so that a moving needle can be seen accurately by using your position to remove parallax. * Based on academic research into teaching online. * Coverage of all of the required practicals (AQA). * Inclusion of background information on each experiment. * Detailed accounts of how to perform the experiment for real or with the simulation. * The simulations have the Association for Science's Green Tick of approval.

Practical NMR Spectroscopy Laboratory Guide is designed to provide non-expert NMR users, typically graduate students in chemistry, an introduction to various facets of practical solution-state NMR spectroscopy. Each chapter offers a series of hands-on exercises, introducing various NMR concepts and experiments and guiding the reader in running these experiments using an NMR spectrometer. The book is written for use with a Bruker NMR spectrometer running TopSpin software versions 1 or 2. This practical resource functions both as a text for instructors of a practical NMR course and also as a reference for spectrometer administrators or NMR facility directors when doing user training. This guide serves as serve as excellent, practical resource on its own or as a companion book to Timothy Claridge's High-Resolution NMR Techniques in Organic Chemistry, 2nd Edition (Elsevier, 2009).

This book is the final part of a trilogy of volumes covering the practical aspects of ligand interactions, biochemistry, and effector mechanisms of signal-transducing receptors. The book focuses on the measurement of receptor binding interactions both at equilibrium and from the kinetic point of view. In addition to descriptions of the most important techniques for the performance of ligand binding assays to both membrane-bound and solubilized receptors. A systematic effort has been made to provide insight into the strategy and tactics of receptor binding assays, and the conditions which must be satisfied if artifacts are to be avoided and interpretable data obtained. Since the behaviuor of even the simplest receptor systems can be surprisingly complex, an attempt has been made to provide some computational tools which allow complications to be predicted and handled or avoided. Ligand binding assays are very widely used, not least in drug screening and development. This volume will help the practitioner to choose an appropriate ligand, make a suitable receptor preparation, design and perform a well thought-out set of assays, and analyse the data obtained correctly.

This spectacularly illustrated book celebrates the structural beauty of everyday materials and the space-age technologies used to probe their surface features and internal structures. It introduces the reader to the various instruments and their uses: scanning electron, ion, and tunneling microscopies, acoustic microscopy and transmission electron microscopy. The book describes how images are processed and analyzed, and how modern materials science is based on these techniques and their ability to "see" materials at the atomic level. The book includes hundreds of illustrations and 32 pages of beautiful color plates depicting the complex microscopic realm within such everyday materials as the metals used in cars and planes, polymer fabrics, ceramics, and the ubiquitous silicon semiconductors, without which society today would fall into disarray and confusion. The many full-color and black-and-white illustrations make this book a pleasure for the eye, in addition to being a useful resource for scientists, students, researchers, and engineers involved in solid-state physics, materials science, geology, and chemistry.

In its simplest form, the scientific method can be thought of as learning from our mistakes and trying to correct them. True scientists try to think rationally, never adopt dogmatic opinions and are always willing to listen to opposing views. They never claim to know the absolute truth but are relentless in their search for it. In this timely book, the author describes the fundamentals of critical scientific thinking. The book further examines the correct use of the scientific method and how to apply it to current events and scientific topics to obtain honest assessments. Current controversies discussed include climate change and COVID-related lockdowns. Additional Features include: Demonstrates the use of the scientific method to assist with objective analysis of issues. Addresses that induction plays a role but the true method for advancing knowledge is hypothesis-deduction. Explores current hot topics within the framework of the scientific method. Outlines common misunderstandings of the scientific method. Applying the Scientific Method to Learn from Mistakes and Approach Truth is approachable enough for the general public and recommended for university and advanced high school science educators and their students.

This volume is the first of its kind on focusing gamma-ray telescopes. Forty-eight refereed papers provide a comprehensive overview of the scientific potential and technical challenges of this nascent tool for nuclear astrophysics. The book features articles dealing with pivotal technologies such as grazing incident mirrors, multilayer coatings, Laue- and Fresnel-lenses - and even an optic using the curvature of space-time.

Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.

In its simplest form, the scientific method can be thought of as learning from our mistakes and trying to correct them. True scientists try to think rationally, never adopt dogmatic opinions and are always willing to listen to opposing views. They never claim to know the absolute truth but are relentless in their search for it. In this timely book, the author describes the fundamentals of critical scientific thinking. The book further examines the correct use of the scientific method and how to apply it to current events and scientific topics to obtain honest assessments. Current controversies discussed include climate change and COVID-related lockdowns. Additional Features include: Demonstrates the use of the scientific method to assist with objective analysis of issues. Addresses that induction plays a role but the true method for advancing knowledge is hypothesis-deduction. Explores current hot topics within the framework of the scientific method. Outlines common misunderstandings of the scientific method. Applying the Scientific Method to Learn from Mistakes and Approach Truth is approachable enough for the general public and recommended for university and advanced high school science educators and their students.

This book focuses on charged-particle optics and microscopy, as well as their applications in the materials sciences. Presenting a range of cutting-edge theoretical and methodological advances in electron microscopy and microanalysis, and examining their crucial roles in modern materials research, it offers a unique resource for all researchers who work in ultramicroscopy and/or materials research. The book addresses the growing opportunities in this field and introduces readers to the state of the art in charged-particle microscopy techniques. It showcases recent advances in scanning electron microscopy, transmission electron microscopy and helium ion microscopy, including advanced spectroscopy, spherical-corrected microscopy, focused-ion imaging and in-situ microscopy. Covering these and other essential topics, the book is intended to facilitate the development of microscopy techniques, inspire young researchers, and make a valuable contribution to the field.

Fermentation: A Practical Approach is a collection of methods and techniques covering the setting up and use of fermentation units in academic research and industrial laboratories. The emphasis is on the breadth of usage of small-scale fermenters and the interdisciplinary nature of fermentation itself. The topics covered include fermentation modelling, sterilization, and instrumentation. This area of research has many important industrial applications, as evidenced by the affiliations of the authors (Hoechst, ICI, Beecham Pharmaceuticals).

The 3rd International Multidisciplinary Microscopy Congress (InterM2015), held from 19 to 23 October 2015, focused on the latest developments concerning applications of microscopy in the biological, physical and chemical sciences at all dimensional scales, advances in instrumentation, techniques in and educational materials on microscopy. These proceedings gather 17 peer-reviewed technical papers submitted by leading academic and research institutions from nine countries and representing some of the most cutting-edge research available.

This volume contains the proceedin,r. of the NATO Advanced Study Institute on "Forces in Scanning Probe Methods which was CG-sponsered and organized by the "Forum fUr N anowissenschaften". The conference was held in Schluchsee in the south- em Black Forest (Germany) from March 7-18, 1994. 30 invited lecturers giving tuto- rial talks of historical and recent research activities and about 100 contributed, oral and poster presentations from 130 people participating, created a very active and lestimulating, lively atmosphere. The inventions of scanning tunneling microscopy, atomic force microscopy and near field optical microsocopy opened a new field of research, called scanning probe meth- ods (SPM). During the last decade, the quality of image acquisition made tremendous progress due to advanced data acquisition systems, low noise electronics and suitable mechan- ical and micromechanical constructions. However, a lot of fundamental, unsolved questions about the interaction between probing tip and sample remain. This vol- ume contains 60 contributions dedicated to these problems. Most of the articles are review articles presenting. condensed and relevant information in a way suitable for both students and specialists. Topics that are covered are instrumental aspects, de- signs of force microscopes in various environments, such as ambient pressure, low temperature, ultrahip vacuum and liquids. An important part of the workshop was dedicated to theory, Including all initio calculations and molecular dynamics simula- tions. Mechanical properties, such as adhesion, friction and wear, on the micrometer and nanometer scale were also treated intensively.

An award-winning science journalist details the quest to isolate and understand dark matter-and shows how that search has helped us to understand the universe we inhabit. When you train a telescope on outer space, you can see luminous galaxies, nebulae, stars, and planets. But if you add all that together, it constitutes only 15 percent of the matter in the universe. Despite decades of research, the nature of the remaining 85 percent is unknown. We call it dark matter. In The Elephant in the Universe, Govert Schilling explores the fascinating history of the search for dark matter. Evidence for its existence comes from a wealth of astronomical observations. Theories and computer simulations of the evolution of the universe are also suggestive: they can be reconciled with astronomical measurements only if dark matter is a dominant component of nature. Physicists have devised huge, sensitive instruments to search for dark matter, which may be unlike anything else in the cosmos-some unknown elementary particle. Yet so far dark matter has escaped every experiment. Indeed, dark matter is so elusive that some scientists are beginning to suspect there might be something wrong with our theories about gravity or with the current paradigms of cosmology. Schilling interviews both believers and heretics and paints a colorful picture of the history and current status of dark matter research, with astronomers and physicists alike trying to make sense of theory and observation. Taking a holistic view of dark matter as a problem, an opportunity, and an example of science in action, The Elephant in the Universe is a vivid tale of scientists puzzling their way toward the true nature of the universe.

This volume focuses on fundamental aspects of nano-electro-optics. Starting with fiber probes and related devices for generating and detecting the optical near-field with high efficiency and resolution, the next chapter addresses the modulation of an electron beam by optical near-fields. Further topics include: fluorescence spectroscopy, in which sample molecules are excited by the evanescent surface plasmon field close to metallic surfaces; spatially resolved near-field photoluminescence spectroscopy of semiconductor quantum dots, which will become an essential issue in future electro-optical devices and systems; and, finally, the quantum theory of the optical near-field. This latter theory accounts for all the essential features of the interaction between optical near-fields and nanomaterials, atoms and molecules. Together these overviews will be a valuable resource for engineers and scientists working in the field of nano-electro-optics.

This book explores how machine learning can be used to improve the efficiency of expensive fundamental science experiments. The first part introduces the Belle and Belle II experiments, providing a detailed description of the Belle to Belle II data conversion tool, currently used by many analysts. The second part covers machine learning in high-energy physics, discussing the Belle II machine learning infrastructure and selected algorithms in detail. Furthermore, it examines several machine learning techniques that can be used to control and reduce systematic uncertainties. The third part investigates the important exclusive B tagging technique, unique to physics experiments operating at the resonances, and studies in-depth the novel Full Event Interpretation algorithm, which doubles the maximum tag-side efficiency of its predecessor. The fourth part presents a complete measurement of the branching fraction of the rare leptonic B decay "B tau nu", which is used to validate the algorithms discussed in previous parts.

Incorporating the latest theoretical and experimental developments in the field over the past decade, Brainina and Neyman's Electroanalytical Stripping Methods focuses on the theory associated with the most progressive stripping electroanalytical methods (SEAMs) and their application to environmental monitoring and industry. Wherever appropriate, it highlights the main advantages of these methods, including their extremely low detection limit, the low cost of the instruments used, the possibility of speciation analysis, and their use in the investigation of solids as well as solutions. The combined work of two eminent researchers from the former Soviet Union, the book closes the previous information gap that existed between West and East and now makes accessible the most recent developments from Russia, including a different approach to initial electro-crystallization stages, the interconnection between voltammograms of binary metal systems and work functions, and the use of these phenomena in applied stripping voltammetry. Electroanalytical Stripping Methods also provides a critical assessment of current achievements in the field of electrode materials, and in the design of electrodes and electrochemical cells used in stripping methods. Here, the authors provide unique insight into the advantages of graphite electrodes - largely used in the East - over glassy carbon electrodes generally favored by the West. The book presents a number of other modern ideas, including the theory behind and the application of a new internal standard in stripping voltammetry...the catalysis of metals electrodeposition on the foreign substrate with adatoms...and the electrochemical transformation of solidsand adsorbates and their use in phase analysis and the investigation of the defect structure of solids. Electroanalytical Stripping Methods will prove to be an invaluable tool for professionals involved in environmental monitoring, helping them to develop and use trace analysis in evaluating drinking water, food, waste, and other solids and solutions and to apply stripping voltammetry to the analyses of chemicals. Researchers in the food, clinical, and pharmaceutical industries will similarly use the book to develop and apply SEAMs for trace analysis. Practitioners in the microelectronics industry will find useful information on solids that will help them improve the quality of catalyzers, films, special ceramics, and semiconductors. Finally, medical technicians will tap this resource for the insight it can provide in the electroanalysis of blood, urine, and other body fluids.

Cell Membrane Nanodomains: From Biochemistry to Nanoscopy describes recent advances in our understanding of membrane organization, with a particular focus on the cutting-edge imaging techniques that are making these new discoveries possible. With contributions from pioneers in the field, the book explores areas where the application of these novel techniques reveals new concepts in biology. It assembles a collection of works where the integration of membrane biology and microscopy emphasizes the interdisciplinary nature of this exciting field. Beginning with a broad description of membrane organization, including seminal work on lipid partitioning in model systems and the roles of proteins in membrane organization, the book examines how lipids and membrane compartmentalization can regulate protein function and signal transduction. It then focuses on recent advances in imaging techniques and tools that foster further advances in our understanding of signaling nanoplatforms. The coverage includes several diffraction-limited imaging techniques that allow for measurements of protein distribution/clustering and membrane curvature in living cells, new fluorescent proteins, novel Laurdan analyses, and the toolbox of labeling possibilities with organic dyes. Since superresolution optical techniques have been crucial to advancing our understanding of cellular structure and protein behavior, the book concludes with a discussion of technologies that are enabling the visualization of lipids, proteins, and other molecular components at unprecedented spatiotemporal resolution. It also explains the ins and outs of the rapidly developing high- or superresolution microscopy field, including new methods and data analysis tools that exclusively pertain to these techniques. This integration of membrane biology and advanced imaging techniques emphasizes the interdisciplinary nature of this exciting field. The array of contributions from leading world experts makes this book a valuable tool for the visualization of signaling nanoplatforms by means of cutting-edge optical microscopy tools.

Safe Storage of Laboratory Chemicals, Second Edition is a comprehensive guide which allows readers to assess and improve the safety of their laboratory operations when storing chemicals. By applying the information in this book, readers can create a safer place to workthrough a safer storage environment, wiser storage practices and procedures, informed personnel, and the intelligent use of information. There has seen a surge in the demand for chemical safety and information relating to a safe workplace. This book covers chemical storage, the requirements of OSHA and EPA, requirements in codes and standards for storage, and advances and applications for chemical health and safety. This book offers a balanced approach to the safe storage of laboratory chemicals. Applied knowledge for identifying chemical storage hazards, solutions and alternative measures for storing specific hazard classes of chemicals, and innovative case histories provide a wealth of information from which readers may draw to enhance the safety of their storage situations.

Volume 16

Reviews In Computational Chemistry

Kenny B. Lipkowitz and Donald B. Boyd

The focus of this book is on methods useful in molecular design. Tutorials and reviews span (1) methods for designing compound libraries for combinatorial chemistry and high throughput screening, (2) the workings of artificial neural networks and their use in chemistry, (3) force field methods for modeling materials and designing new substances, and (4) free energy perturbation methods of practical usefulness in ligand design.

From Reviews of the Series

"This series spans all the subdisciplines in the field, from techniques to practical applications, and includes reviews from many of the acknowledged leaders in the field. the reviews cross many subdisciplines yet are both general enough to be of wide interest while including detailed information of use to workers in particular subdisciplines." –Journal of the American Chemical Society

Radionuclides produced by past nuclear weapon test explosions comprise the largest source of anthropogenic radioactivity released into the earth's atmosphere to date. This volume presents data and models about the fate of the released radionuclides and their possible effects on human health. It is divided into the following three parts: - Source Term Studies;- Dose Reconstruction;- Ecological and Health Effects,and comprises both Western and formerly secret Soviet research studies, illuminates past and current research.