This book is focused on the current status of industrial pollution, its source, characteristics, and management through various advanced treatment technologies. The book covers the recycle, reuse and recovery of waste for the production of value-added products. The book explores industrial wastewater pollution and its treatment through various advanced technologies and also the source and characteristics of solid waste and its management for environmental safety. It discusses new methods and technologies to combat the waste-related pollution and focuses on the use of recycled products. This book is of value to upcoming students, researchers, scientists, industry persons and professionals in the field of environmental science and engineering, microbiology, biotechnology, toxicology, further it is useful for global and local authorities and policy makers responsible for the management of liquid and solid wastes.

This volume results from a conference jointly sponsored by the Biotechnology Unit of the Government Chemist and the Society for Applied Bacteriology. ATP (Adenosine TriPhosphate - the basic molecule of energy) luminscence technology is an effective and rapid method for assaying microbial numbers. Although developed in the 1970s, only recently has the method been made reliable. This volume covers a broad range of topics concerned with this form of rapid microbiology. It contains detailed coverage of the most recent advances made in this area edited by the major authorities in the field.

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

In the 1980's sonochemistry was considered to be a rather restricted branch of chemistry involving the ways in which ultrasound could improve synthetic procedures, predominantly in heterogeneous systems and particularly for organometallic reactions. Within a few years the subject began to expand into other disciplines including food technology, environmental protection and the extraction of natural materials. Scientific interest grew and led to the formation of the European Society of Sonochemistry in 1990 and the launch of a new journal Ultrasonics Sonochemistry in 1994. The subject continues to develop as an exciting and multi-disciplinary science with the participation of not only chemists but also physicists, engineers and biologists. The resulting cross-fertilisation of ideas has led to the rapid growth of interdisciplinary research and provided an ideal way for young researchers to expand their knowledge and appreciation of the ways in which different sciences can interact. It expands scientific knowledge through an opening of the closed doors that sometimes restrict the more specialist sciences. The journey of exploration in sonochemistry and its expansion into new fields of science and engineering is recounted in "Sonochemistry Evolution and Expansion" written by two pioneers in the field. It is unlike other texts about sonochemistry in that it follows the chronological developments in several very different applications of sonochemistry through the research experiences of the two authors Tim Mason and Mircea Vinatoru. Designed for chemists and chemical engineers Written by two experts and practitioners in the subject Volume 1 covers the historical background and evolution of sonochemistry Volume 2 explains the wider applications and expansion of the subject VOLUME 1 Fundamentals and Evolution This volume traces the evolution of sonochemistry from the very beginning when the effects of acoustic cavitation were first reported almost as a scientific curiosity. The major developments of the subject from the 1980's are described by the authors who became active participants in the field during that period. A chapter is devoted to ultrasonically assisted extraction (UAE) which illustrates the different ways in which sonochemical technologies can be applied in both batch and flow modes leading to the development of large-scale processing. The chapter on environmental protection shows the wide range of applications of sonochemistry in this important field for both biological and chemical decontamination.

Spark scientific curiosity from a young age with this six-level course through an enquiry-based approach and active learning. Collins International Primary Science fully meets the requirements of the Cambridge Primary Science Curriculum Framework from 2020 and has been carefully developed for a range of international contexts. The course is organised into four main strands: Biology, Chemistry, Physics and Earth and Space and the skills detailed under the ‘Thinking and Working Scientifically’ strand are introduced and taught in the context of those areas. For each Teacher’s Guide at Stages 1 to 6, we offer: A comprehensive Teacher’s Guide is easy to follow with a clear and consistent lesson plan layout, including built in continuous assessment The Teacher’s Guide Plus ebook includes components such as slideshows, video clips, additional photographs and interactive activities Earth and Space content covers the new curriculum framework Thinking and Working Scientifically deepens and enhances the delivery of Science skills Includes practical activities that don’t require specialist equipment or labs Scaffolding allows students of varying abilities to work with common content and meet learning objectives Supports Cambridge Global Perspectives™ with activities that develop and practise key skills Provides teacher support as part of a set of resources for the Cambridge Primary Science curriculum framework (0097) from 2020 This series is endorsed by Cambridge Assessment International Education to support the new curriculum framework 0097 from 2020.

This completely revised edition explores novel discoveries in bacterial genomic research, with a focus on technical and computational improvements as well as methods used for bacterial pangenome analysis, which relies on microbiome studies and metagenomic data. Beginning with up-to-date sequencing methods, the book continues with sections covering methods for deep phylogenetic analysis, the role of metagenomic data in understanding the genomics of the many yet uncultured bacteria, progress in genome-to-phenome inference, as well as computational genomic tools. Written for the highly successful Methods in Molecular Biology series, chapters include the type of practical detail necessary for reproducible results in the lab. Authoritative and up-to-date, Bacterial Pangenomics: Methods and Protocols, Second Edition serves as an ideal guide for both highly qualified investigators in bacterial genomics and for less experienced researchers, including students and teachers, who could use a reference for approaching genomic analysis and genome data.

Since its discovery, Atomic Force Microscopy (AFM) has become a technique of choice for non-destructive surface characterization with sub-molecular resolution. The AFM has also emerged as a problem-solving tool in applications relevant to particle-solid and particle-liquid interactions, design, fabrication, and characterization of new materials, and development of new technologies for processing and modification of materials. This volume is a comprehensive review of AFM techniques and their application in adhesion studies. It is intended for both researchers and students in engineering disciplines, physics and biology. Over 100 authors contributed to this book, summarizing current status of research on measurements of colloidal particle-solid adhesion and molecular forces, solid surface imaging and mapping, and discussing the contact mechanics models applicable to particle-substrate and particle-particle systems.

A time-tested, systematic approach to the buying and selling of complex research instruments

Searching for the best laboratory instruments and systems can be a daunting and expensive task. A poorly selected instrument can dramatically affect results produced and indirectly affect research papers, the quality of student training, and an investigator's chances for advancement. "Buying and Selling Laboratory Instruments" offers the valuable insights of an analytical chemist and consultant with over four decades of experience in locating instruments based upon both need and price. It helps all decision makers find the best equipment, service, and support while avoiding the brand-loyalty bias of sales representatives so you can fully meet your laboratory's requirements.

The first section of the book guides buyers through the hurdles of funding, purchasing, and acquiring best-fit instruments at the least-expensive price. It explains how to find vendors that support their customers with both knowledgeable service and application support. Also offered is guidance on adapting your existing instruments to new applications, integrating new equipment, and what to do with instruments that can no longer serve in research mode.

The second section explains the sales process in detail. This is provided both as a warning against manipulative sales reps and as a guide to making the sale a win-win process for you and your vendor. It also shows you how to select a knowledgeable technical guru to help determine the exact system configuration you need and where to find the best price for it. Added bonuses are summary figures of buying sequence and sales tools and an appendix containing frequently asked questions and memory aids.

"Buying and Selling Laboratory Instruments" is for people directly involved in selecting and buying instruments for operational laboratories, from the principle investigator to the person actually delegated with investigating and selecting the system to be acquired. Sales representatives; laboratory managers; universities; pharmaceutical, biotech, and forensic research firms; corporate laboratories; graduate and postdoctoral students; and principle investigators will not want to be without this indispensible guide.

Stereology, or quantitative microscopy, is a basic research tool in science and technology. The emergence of design-based methods has greatly increased the power, flexibility, adaptability, and scope of stereology applications, establishing a closer connection between statistics and quantitative microscopy. Despite its scientific importance, modern stereology remains largely unknown to the statistical community, with valuable information either widely scattered or inaccessible to newcomers to the field. Now is the perfect time for a book that enables biostatisticians and statistical consultants to give beneficial advice to researchers in microscopy.

Stereology for Statisticians sets out the principles of stereology from a statistical viewpoint, focusing on both basic theory and practical implications. This book discusses ways to effectively communicate statistical issues to clients, draws attention to common methodological errors, and provides references to essential literature.

The first full text on design-based stereology, it opens with a review of classical and modern stereology, followed by a treatment of mathematical foundations such as geometry, probability, and statistical inference. The book then presents core techniques, including estimation of absolute geometrical quantities, relative quantities, and statistical inference for populations of discrete objects. The final chapters discuss implementing techniques in practical sampling designs, summarize understanding of the variance of stereological estimators, and describe open problems for further research.

The Sunday Times Top Ten Bestseller Have you ever wondered if a severed head retains consciousness long enough to see what happened to it? Or whether your dog would run to fetch help, if you fell down a disused mineshaft? And what would happen if you were to give an elephant the largest ever single dose of LSD? The chances are that someone, somewhere has conducted a scientific experiment to find out... 'Excellent accounts of some of the most important and interesting experiments in biology and psychology' Simon Singh If left to their own devices, would babies instinctively choose a well-balanced diet? Discover the secret of how to sleep on planes Which really tastes better in a blind tasting - Coke or Pepsi?

Image processing is fast becoming a valuable tool for analyzing multidimensional data in all areas of natural science. Since the publication of the best-selling first edition of this handbook, the field of image processing has matured in many of its aspects from ad hoc, empirical approaches to a sound science based on established mathematical and physical principles. The Practical Handbook on Image Processing for Scientific and Technical Applications, Second Edition builds a sound basic knowledge of image processing, provides a critically evaluated collection of the best algorithms, and demonstrates those algorithms with real-world applications from many fields. It covers all aspects of image processing, from image formation to image analysis, and gives an up-to-date review of advanced concepts. Organized according to the hierarchy of tasks, each chapter includes a summary, an outline of the background the task requires, and a section of practical tips that help you avoid common errors and save valuable research time. New in the Second Edition: Expanded application areas now include technical fields such as the automotive industry, quality inspection, and materials science More practical tips in each chapter Discussion of digital camera interfaces and a comparison of CMOS vs. CCD cameras A section on wavelets Advanced techniques for reconstruction, new segmentation methods based on global optimization and anisotropic diffusion, and additional classification techniques, including neural networks, polynomial classification, and support vector machines Just as digital image processing provides the key to studying complex scientific problems that researchers once could only dream of tackling, this handbook unlocks the intricacies of image processing and brings its power and potential within the grasp of researchers across the spectrum of scientific and engineering disciplines.

Although methods and techniques that will help solve various analytical problems do exist, they are often difficult to perform. Using polarized light microscopy as the method of choice, Color Atlas and Manual of Microscopy for Criminalists, Chemists, and Conservators offers swift, simple, yet irrefutable analytical tests and testing procedures that can be used to identify organic and inorganic particles. Seasoned forensic microscopists Nicholas Petraco and Thomas Kubic have lent their expertise as consultants to forensic scientists, analytical chemists, art historians, pathologists, customs agents, detectives, gemologists, numismatists, and art conservators. Now they share their extensive photomicrograph collection of minute specimens along with clear, concise, and simple methods to help solve your analytical problems.

There is currently a high level of interest in Laboratory Information Management Systems (LIMS), which, when successfully implemented, can revitalize the operations of a laboratory and contribute significantly to the effectiveness and efficiency of the overall enterprise. LIMS describes the strategy, planning, resources, and activities needed to integrate LIMS and its supporting technologies into an organization. It covers all aspects of implementation and management and has the benefit of not being product specific. This book will not date as it is not restricted to a particular software product, hardware platform, or technical automation approach. Instead it deals with the issues, expertise, organization, and resources that contribute to the successful implementation of LIMS. The author has wide experience of automated laboratory systems in the chemical, pharmaceutical, environmental, and biotechnology industries, and for the past 15 years has been intimately involved in every aspect of LIMS implementations including justification, system selection, installation, project management, developing, training, validation, performance optimization, and maintenance. LIMS contains numerous illustrations and tables to highlight concisely the major points and concepts discussed in each chapter. The book is essential reading for laboratory, information systems and project managers responsible for the implementation of LIMS and, as it does not require any previous knowledge of computers or laboratory information management systems, is easily accessible to all.

This book presents a detailed look at experimental and computational techniques for accurate structure determination of free molecules. The most fundamental property of a molecule is its structure - it is a prerequisite for determining and understanding most other important properties of molecules. The determination of accurate structures is hampered by a myriad of factors, subjecting the collected data to non-negligible systematic errors. This book explains the origin of these errors and how to mitigate and even avoid them altogether. It features a detailed comparison of the different experimental and computation methods, explaining their interplay and the advantages of their combined use. Armed with this information, the reader will be able to choose the appropriate methods to determine - to a great degree of accuracy - the relevant molecular structure.

I In this volume, the author demystifies the Design of Experiments (DOE). He begins with a clear explanation of the traditional experimentation process. He then covers the concept of variation and the importance of experimentation and follows through with applications. Stamatis also discusses full and fractional factorials. The strength of this volume lies in the fact that not only does it introduce the concept of robustness, it also addresses "Robust Designs" with discussions on the Taguchi methodology of experimentation. And throughout the author ties these concepts into the Six Sigma philosophy and shows readers how they use those concepts in their organizations.

This book discusses fundamentally new biomedical imaging methods, such as holography, holographic and resonant interferometry, and speckle optics. It focuses on the development of holographic interference microscopy and its use in the study of phase objects such as nerve and muscle fibers subjected to the influence of laser radiation, magnetic fields, and hyperbaric conditions. The book shows how the myelin sheath and even the axon itself exhibit waveguide properties, enabling a fresh new look at the mechanisms of information transmission in the human body. The book presents theoretically and experimentally tested holographic and speckle-optical methods and devices used for investigating complex, diffusely scattering surfaces such as skin and muscle tissue. Additionally, it gives broad discussion of the authors' own original fundamental and applied research dedicated to helping physicians introduce new contact-less methods of diagnosis and treatment of diseases of the cardiovascular and neuromuscular systems into medical practice. The book is aimed at a broad spectrum of scientific specialists in the fields of speckle optics, holography, laser physics, morphology and cytochemistry, as well as medical professionals such as physiologists, neuropathologists, neurosurgeons, cardiologists and dentists.

Toxicology has made tremendous strides in the sophistication of the models used to identify and understand the mechanisms of agents that can harm or kill humans and other higher organisms. Non-animals or in vitro models started to gain significant use in the 1960s. As a result of the increased concern over animal welfare, economic factors, and the need for greater sensitivity and understanding of mechanisms, interest in in vitro models has risen. This volume demonstrates that there now exists a broad range of in vitro models for use in either identifying or understanding most forms of toxicity. The availability of in vitro models spans both the full range of endpoints (irritation, sensitization, lethality, mutagenicity, and developmental toxicity) and the full spectrum of target organ systems (including the skin, eye, heart, liver, kidney and nervous system). Chapters are devoted to each of these speciality areas from a perspective of presenting the principal models and their uses and limitations.

Private landowners or Federal Agencies responsible for cleaning up radiological environments are faced with the challenge of clearly defining the nature and extent of radiological contamination, implementing remedial alternatives, then statistically verifying that cleanup objectives have been met. Sampling and Surveying Radiological Environments provides the how-tos for designing and implementing cost effective and defensible sampling programs in radiological environments, such as those found in the vicinity of uranium mine sites, nuclear weapons production facilities, nuclear reactors, radioactive waste storage and disposal facilities, and nuclear accidents. It includes downloadable resources that walk you through the EPA's Data Quality Objectives(DQO) procedures and provides electronic templates you can complete and print. Sampling and Surveying Radiological Environments addresses all of the major topics that will assist you in designing and implementing statistically defensible sampling programs in radiological environments, including: Summary of the major environmental laws and regulations that apply to radiological sites, and advice on regulatory interfacing * Internet addresses where you can find regulations pertaining to each States Theory of radiation detection and definitions of common radiological terminology Statistics and statistical software that apply to the environmental industry Details on commercially available radiological instrumentation and detection systems Building decontamination and decommissioning, radiological and chemical equipment decontamination procedures, and tank/drum/remote characterization Standard operating procedures for collecting environmental media samples Guidance on sample preparation, documentation, and shipment Guidance on data verification/validation, radiological data management, data quality assessment (DQA)

'G. Adams in Fleet Street London' is the signature on some of the finest scientific instruments of the eighteenth century. This book is the first comprehensive study of the instrument-making business run by the Adams family, from its foundation in 1734 to bankruptcy in 1817. It is based on detailed research in the archival sources as well as examination of extant instruments and publications by George Adams senior and his two sons, George junior and Dudley. Separate chapters are devoted to George senior's family background, his royal connections, and his new globes; George junior's numerous publications, and his dealings with van Marum; and to Dudley's dabbling with 'medico-electrical therapeutics'. The book is richly illustrated with plates from the Adams's own publications and with examples of instruments ranging from unique museum pieces - such as the 'Prince of Wales' microscope - and globes to the more common, even mundane, items of the kind seen in salesrooms and dealers - the surveying, navigational and military instruments that formed the backbone of the business. The appendices include facsimiles of trade catalogues and an annotated short-title listing of the Adams family's publications, which also covers American and Continental editions, as well as the posthumous ones by W. & S. Jones.

Experts from The Jackson Laboratory and around the world provide practical advice on everything from how to establish a colony to where to go for specific mutations. Systematic Approach to Evaluation of Mouse Mutations includes information on medical photography, grafting procedures, how to map the genes and evaluate the special biological characteristics of the mice.

Since the initial discovery of the G protein-coupled receptor system that regulates cyclicAMP production, the G protein field has rapidly expanded. Cell surface receptors that couple to heterotrimeric G proteins, the G prote- coupled receptors (GPCRs), number in the hundreds and bind to a wide div- sity of ligands including, biogenic amines (e. g. , adrenaline), lipid derivatives (e. g. , lysophosphatidic acid), peptides (e. g. , opioid peptides), proteins (e. g. , thyroid-stimulating hormone), and odorants to name a few. The GPCR system is found throughout biology in such simple organisms as yeast and in such more complex organisms as Dictyostelium discoideum (slime mold), Caen- habditis elegans (nematode worm), and of course in humans. GPCRs and their associated G protein systems are the subject of intense academic research and because of their involvement in a human biology and disease, the pharmac- tical industry has large research initiatives dedicated to the study of GPCRs. By some estimates, more than 50% of the pharmaceuticals on the market are targeted at GPCRs. The G protein/G protein-coupled receptor system consists of a receptor (GPCR), a heterotrimeric G protein consisting of ?, ?, and ? subunits, and an effector. G protein effector molecules, such as enzymes or ion channels, respond to acti- tion by the G protein to generate second messengers or changes in membrane potential that lead to alterations in cell physiology.

The goal of this book is to make some underutilized but potentially very useful methods in experimental design and analysis available to ecologists, and to encourage better use of standard statistical techniques. Ecology has become more and more an experimental science in both basic and applied work, but experiments in the field and in the laboratory often present formidable statistical difficulties. Organized around providing solutions to ecological problems, this book offers ways to improve the statistical aspects of conducting manipulative ecological experiments, from setting them up to interpreting and reporting the results. An abundance of tools, including advanced approaches, are made available to ecologists in step-by-step examples, with computer code provided for common statistical packages. This is an essential how-to guide for the working ecologist and for graduate students preparing for research and teaching careers in the field of ecology.

It is now more than ten years since Dr. Alec Jeffreys (now Professor Sir Alec Jeffreys, FRS) reported in Nature that the investigation of certain minisatellite regions in the human genome could produce what he termed DNA fingerprints and provide useful information in the fields of paternity testing and forensic analysis. Since that time we have witnessed a revolution in the field of forensic identification. A total change of technology, from serological or electrophoretic analysis of protein polymorphisms to direct investigation of the underlying DNA polymorphisms has occurred in a short space of time. In addition, the evolution and development of the DNA systems themselves has been rapid and spectacular. In the last decade we have progressed from the multilocus DNA fing- prints, through single locus systems based on the same Southern blot RFLP technology, to a host of systems based on the PCR technique. These include Allele Specific Oligonucleotide (ASO)-primed systems detected by dot blots, the "binary" genotypes produced by mapping variations within VNTR repeats demonstrated by minisatellite variant repeat (MVR) analysis, and yet other fragment-length polymorphisms in the form of Short Tandem Repeat (STR) loci. Hand in hand with the increasing range of systems available has been the development of new instrumentation to facilitate their analysis and allow us to explore the possibilities of high volume testing in the form of mass scre- ing and offender databases.

Offering all aspects of humidity measurement and instrumentation, this work includes rudiments and theory, common applications, advantages and limitations of frequently-used sensors and techniques, and guidelines for installation, maintenance and calibration. The disk is intended for easy conversions of humidity parameters and units.

Response Surfaces: Designs and Analyses; Second Edition presents techniques for designing experiments that yield adequate and reliable measurements of one or several responses of interest, fitting and testing the suitability of empirical models used for acquiring information from the experiments, and for utilizing the experimental results to make decisions concerning the system under investigation.
This edition contains chapters on response surface models with block effects and on Taguchi's robust parameter design, additional details on transformation of response variable, more material on modified ridge analysis, and new design criteria, including rotatability for multiresponse experiments. It also presents an innovative technique for displaying correlation among several response. Numerical examples throughout the book plus exercises--with worked solutions to selected problems--complement the text.