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Books > Science & Mathematics > Science: general issues > Scientific equipment & techniques, laboratory equipment

Signal Transduction - Single Cell Techniques (Paperback, 1998 ed.): Bert Van Duijn, Anneke Wiltink Signal Transduction - Single Cell Techniques (Paperback, 1998 ed.)
Bert Van Duijn, Anneke Wiltink
R2,975 Discovery Miles 29 750 Ships in 10 - 15 working days

A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.

Vacuum Microbalance Techniques - Volume 7: Proceedings of the Eindhoven Conference June 17-18, 1968 (Paperback, Softcover... Vacuum Microbalance Techniques - Volume 7: Proceedings of the Eindhoven Conference June 17-18, 1968 (Paperback, Softcover reprint of the original 1st ed. 1970)
C. H. Massen, H. J. Van Beckum
R1,507 Discovery Miles 15 070 Ships in 10 - 15 working days

Volume 7 Proceedings of the Eindhoven Conference June 1718 1968.

Vacuum Microbalance Techniques - Volume 8 Proceedings of the Wakefield Conference, June 12-13, 1969 (Paperback, Softcover... Vacuum Microbalance Techniques - Volume 8 Proceedings of the Wakefield Conference, June 12-13, 1969 (Paperback, Softcover reprint of the original 1st ed. 1971)
A.W. Czanderna
R1,510 Discovery Miles 15 100 Ships in 10 - 15 working days

This volume contains the proceedings of the Eighth Conference on Vacuum Microbalance Techniques held at Wakefield, Massachusetts on June 12 and 13, 1969. The tenth anniversary of the first confer ence will be registered as this volume passes through the typeset ting and proofreading stages. The eight volumes that have spawned from this continuing series of conferences now contain a total of 125 papers. Thus, these volumes serve as a major repository of the world's literature on vacuum microbalance techniques. The Ninth and Tenth Conferences will be held in West Germany in June 1970 and in Texas in 1971. Each of the eight meetings has served as a forum where new developments in this rapidly advancing field can be presented and discussed constructively within a conference atmosphere of cordial informality. The interaction of the participants at the conferences has led to the first treatise on ultra mlcrogravtmetry;' edited by S. P. Wolsky and E. J. Zdanuk, with most of the fourteen chapters written by steady contributors to the volumes on Vacuum Micro balance Techniques. The number of research investigations and published works in which a vacuum microbalance is utilized con tinues to expandr apldly. f This is a direct result of several types of automatic recording balances that are now available commercial ly. 3 The Eighth Conference was held to bring together again re search scientists and engineers who exploit the measurement of mass as a means of studying physical and chemical phenomena.

Vacuum Manual (Paperback, Softcover reprint of the original 1st ed. 1974): L. Holland Vacuum Manual (Paperback, Softcover reprint of the original 1st ed. 1974)
L. Holland
R1,605 Discovery Miles 16 050 Ships in 10 - 15 working days

Vacuum apparatus is widely used in research and industrial establishments for providing and monitoring the working environments required for the operation of many kinds of scientific instruments and process plant. The vacuum conditions needed range from the relatively coarse vacuum requirements in applications covering diverse fields such as food packaging, dentistry (investment casting), vacuum forming, vacuum metallur gical processes, vacuum impregnation, molecular distillation, vacuum drying and freeze drying etc. to the other extreme involving the highest possible vacuum as in particle accelerators, space technology -both in simulation and outer space, and research studies of atomically clean surfaces and pure condensed metal films. Vacua commence with the rough vacuum region, i.e. from atmosphere to 100 Pa * passing 6 through medium vacuum of 100 Pa to 0.1 Pa and high vacuum of 0.1 Pa to 1 J.lPa (10- Pa) until ultra high vacuum is reached below 1 J.lPa to the limit of measurable pressure about 12 I pPa (10- Pa)."

Conversas com a Inteligencia Artificial - 111 Perguntas Artificial Intelligence for Thinking Humans (Portuguese, Hardcover,... Conversas com a Inteligencia Artificial - 111 Perguntas Artificial Intelligence for Thinking Humans (Portuguese, Hardcover, Primeira Edicao ed.)
Ingrid Seabra, Pedro Seabra, Angela Chan
R812 R712 Discovery Miles 7 120 Save R100 (12%) Ships in 10 - 15 working days
Total and Partial Pressure Measurement in Vacuum Systems (Paperback, Softcover Repri): John Henry Leck Total and Partial Pressure Measurement in Vacuum Systems (Paperback, Softcover Repri)
John Henry Leck
R1,494 Discovery Miles 14 940 Ships in 10 - 15 working days

This book deals with the underlying theory and practical aspects of pressure gauges that are at present in general use. Because of the ever-increasing demands to provide a wider range of sophisticated and reliable vacuum equipment a good understanding of these instruments is of vital importance to all workers in the research and industrial sectors. Of the gauges considered only the mechanical types are absolute, in the sense that they measure pressure directly as a force upon a liquid column or a solid surface. Under ideal conditions it is possible to calculate their sensitiv ities, which are the same for all gases and vapours. The recent developments in the viscous or molecular damping gauges indicate that these may also be considered absolute. Other gauges are indirect in that they involve the measurement of some secondary phenomenon which is pressure-dependent and therefore these gauges can only be used for measurement after calibration against an absolute standard. The radiometer or Knudsen type gauge has been excluded from the text since these are now only of historic interest. Also no mention is made of the integration techniques involving surface changes (such as work function) although these could have application under very special circumstances. The McLeod gauge is dealt with in some detail, for even though this gauge has few practical applications, it is the most sensitive absolute gauge available and has value as a reference standard."

X-ray Microscopy - Instrumentation and Biological Applications (Paperback, Softcover reprint of the original 1st ed. 1987):... X-ray Microscopy - Instrumentation and Biological Applications (Paperback, Softcover reprint of the original 1st ed. 1987)
Ping-Chin Cheng, Gwo-jen Jan
R2,931 Discovery Miles 29 310 Ships in 10 - 15 working days

In 1979, a conference on x-ray microscopy was organized by the New York Academy of Sciences, and in 1983, the Second Interna tional Symposium on X-ray Imaging was organized by the Akademie der Wissenschaften in Gottingen, Federal Republic of Germany. This volume contains the contributions to the symposium "X-ray Microscopy '86," held in Taipei, Taiwan, the Republic of China in August 1986. This is the first volume which intends to provide up-to date information on x-ray imaging to biologists, therefore, emphasis was given to specimen preparation techniques and image interpreta tion. Specimen preparation represents a major part of every microscopy work, therefore, it should be strongly emphasized in this emerging field of x-ray microscopy. Theoretically, x-ray microscopy offers the potential for the study of unfixed, hydrated biological ma terials. Since very few biological system can be directly observed without specimen preparation, we would like to emphasize that new information on biological specimens can only be obtained if the speci men is properly prepared. In the past decade, many of the published x-ray images were obtained from poorly prepared biological speci mens, mainly air-dried materials. Therefore, one of the goals of this conference is to bring the importance of specimen preparation to the attention of x-ray microscopy community. X-ray microscopy can be subdivided into several major areas. They are the classic x-ray projection microscope, x-ray contact imag ing (microradiography) and the more recent x-ray scanning micro scope, x-ray photoelectron microscope and x-ray imaging microscope."

Acoustic Scanning Probe Microscopy (Paperback, 2013 ed.): Francesco Marinello, Daniele Passeri, Enrico Savio Acoustic Scanning Probe Microscopy (Paperback, 2013 ed.)
Francesco Marinello, Daniele Passeri, Enrico Savio
R4,314 Discovery Miles 43 140 Ships in 10 - 15 working days

The combination of atomic force microscopy with ultrasonic methods allows the nearfield detection of acoustic signals. The nondestructive characterization and nanoscale quantitative mapping of surface adhesion and stiffness or friction is possible. The aim of this book is to provide a comprehensive review of different scanning probe acoustic techniques, including AFAM, UAFM, SNFUH, UFM, SMM and torsional tapping modes. Basic theoretical explanations are given to understand not only the probe dynamics but also the dynamics of tip surface contacts. Calibration and enhancement are discussed to better define the performance of the techniques, which are also compared with other classical techniques such as nanoindentation or surface acoustic wave. Different application fields are described, including biological surfaces, polymers and thin films.

Monte Carlo Modeling for Electron Microscopy and Microanalysis (Hardcover, New): David C. Joy Monte Carlo Modeling for Electron Microscopy and Microanalysis (Hardcover, New)
David C. Joy
R4,946 Discovery Miles 49 460 Ships in 12 - 19 working days

This book describes for the first time how Monte Carlo modeling methods can be applied to electron microscopy and microanalysis. Computer programs for two basic types of Monte Carlo simulation are developed from physical models of the electron scattering process--a single scattering program capable of high accuracy but requiring long computation times, and a plural scattering program which is less accurate but much more rapid. Optimized for use on personal computers, the programs provide a real time graphical display of the interaction. The programs are then used as the starting point for the development of programs aimed at studying particular effects in the electron microscope, including backscattering, secondary electron production, EBIC and cathodo-luminescence imaging, and X-ray microanalysis. The computer code is given in a fully annotated format so that it may be readily modified for specific problems. Throughout, the author includes numerous examples of how such applications can be used. Students and professionals using electron microscopes will want to read this important addition to the literature.

RNP Particles, Splicing and Autoimmune Diseases (Paperback, 1998 ed.): Johannes Schenkel RNP Particles, Splicing and Autoimmune Diseases (Paperback, 1998 ed.)
Johannes Schenkel
R1,504 Discovery Miles 15 040 Ships in 10 - 15 working days

The first insights into the site and mechanisms of RNA process- ing to functional mRNA in eukaryotic cells came from the group of Georgiev (Lukanidin et al. 1972) who demonstrated the association of rapidly labelled, heterogeneous nuclear RNA (hnRNA) with a limited number of specific proteins in the cell nucleus. These "informofers", i. e. packaged precursors of mRNA (pre-mRNA or hnRNA), are in a form presumably amenable to the action of nucleases. With the availability of better analytical techniques, the considerable heterogeneity of hnRNA associated proteins was revealed (Niessing and Sekeris 1970), suggesting a role that was more composite, rather than solely structural, for these proteins. Later studies investigated the RNA binding behavior of these proteins (Schenkel et al. 1988, 1989; Wilk et al. 1983). For a long time, the small nuclear RNAs, well characterized with respect to primary structure (reviewed by Reddy and Busch 1983), were naively ignored regarding their function. Several events then set the stage for a detailed study of the intricate mechanisms of the splicing process and other steps involved in hnRNA processing: (1) The demonstration of a second class of nuclear ribonucleoproteins (RNPs), composed of small nuclear RNAs (snRNAs) and another characteristic group ofheterogene- ous proteins (Lerner et al. 1980; Guialis et al. 1983); (2) the detec- tion of the association of snRNPs with hnRNPs by virtue of base pairing between hnRNA and snRNA (Flytzanis et al.

Cytoskeleton Proteins - A Purification Manual (Paperback, 1995 ed.): Gerhard Isenberg Cytoskeleton Proteins - A Purification Manual (Paperback, 1995 ed.)
Gerhard Isenberg
R1,516 Discovery Miles 15 160 Ships in 10 - 15 working days

The standard protocols for the purification of all known cytoskeleton proteins are presented in this manual. Proteins are listed alphabetically and each protocol follows a common format. Thus, the manual provides a quick and easy reference to all relevant procedures for cytoskeleton protein purification.
The isolation procedure for each protein is shown in a clear flowchart, while the source of the protein, equipment and material needed, a list of suppliers, standard references, accession No. of sequences as well as further relevant facts and practical tips are given on a separate page.

New Horizons of Applied Scanning Electron Microscopy (Paperback, 2010 ed.): Kenichi Shimizu, Tomoaki Mitani New Horizons of Applied Scanning Electron Microscopy (Paperback, 2010 ed.)
Kenichi Shimizu, Tomoaki Mitani
R2,845 Discovery Miles 28 450 Ships in 10 - 15 working days

In modern scanning electron microscopy, sample surface preparation is of key importance, just as it is in transmission electron microscopy. With the procedures for sample surface preparation provided in the present book, the enormous potential of advanced scanning electron microscopes can be realized fully. This will take the reader to an entirely new level of scanning electron microscopy and finely-detailed images never seen before.

Studying Kinetics with Neutrons - Prospects for Time-Resolved Neutron Scattering (Paperback, 2010 ed.): Goetz Eckold, Helmut... Studying Kinetics with Neutrons - Prospects for Time-Resolved Neutron Scattering (Paperback, 2010 ed.)
Goetz Eckold, Helmut Schober, Stephen E. Nagler
R4,346 Discovery Miles 43 460 Ships in 10 - 15 working days

Neutrons are extremely versatile probes for investigating structure and dynamics in condensed matter. Due to their large penetration depth, they are ideal for in-situ measurements of samples situated in sophisticated and advanced environments. The advent of new high-intensity neutron sources and instruments, as well as the development of new real-time techniques, allows the tracking of transformation processes in condensed matter on a microscopic scale. The present volume provides a review of the state of the art of this new and exciting field of kinetics with neutrons.

Fascinating Problems for Young Physicists - Discovering Everyday Physics Phenomena and Solving Them (Hardcover): Nenad... Fascinating Problems for Young Physicists - Discovering Everyday Physics Phenomena and Solving Them (Hardcover)
Nenad Vukmirovic, Vladimir Veljic
R2,082 Discovery Miles 20 820 Ships in 12 - 19 working days

Problem-solving is the cornerstone of all walks of scientific research. Fascinating Problems for Young Physicists attempts to clear the boundaries of seemingly abstract physical laws and their tangible effects through a step-by-step approach to physics in the world around us. It consists of 42 problems with detailed solutions, each describing a specific, interesting physical phenomenon. Each problem is further divided into questions designed to guide the reader through, encouraging engagement with and learning the physics behind the phenomenon. By solving the problems, the reader will be able to discover, for example, what the relation is between the mass of an animal and its expected lifetime, or what the efficiency limit is of wind turbines. Intended for first-year undergraduate students and interested high school students, this book develops inquiry-based scientific practice and enables students to acquire the necessary skills for applying the laws of physics to realistic situations.

Linear and Chiral Dichroism in the Electron Microscope (Hardcover): Peter Schattschneider Linear and Chiral Dichroism in the Electron Microscope (Hardcover)
Peter Schattschneider
R3,585 Discovery Miles 35 850 Ships in 12 - 19 working days

This book describes energy loss magnetic chiral dichroism (EMCD), a phenomenon in energy loss spectroscopy discovered in 2006. EMCD is the equivalent of XMCD but is based on fast probe electrons in the electron microscope. A spatial resolution of 2 nm has been demonstrated, and the lattice-resolved mapping of atomic spins appears feasible. EMCD is, thus, a promising technique for magnetic studies on the nanometer and sub-nanometer scale, providing the technical and logistic advantages of electron microscopy, such as in situ chemical and structural information, easy access, and low cost.

Conversas com a Inteligencia Artificial - A Modern Approach to Age Old Questions (Portuguese, Hardcover, Primeira Edicao ed.):... Conversas com a Inteligencia Artificial - A Modern Approach to Age Old Questions (Portuguese, Hardcover, Primeira Edicao ed.)
Ingrid Seabra, Pedro Seabra, Angela Chan
R962 R823 Discovery Miles 8 230 Save R139 (14%) Ships in 10 - 15 working days
Advanced Computer Simulation Approaches for Soft Matter Sciences I (Paperback, Softcover reprint of hardcover 1st ed. 2005):... Advanced Computer Simulation Approaches for Soft Matter Sciences I (Paperback, Softcover reprint of hardcover 1st ed. 2005)
Christian Holm; Contributions by Sauer; Edited by Kurt Kremer; Contributions by K. Binder, J.G. Curro, …
R5,575 Discovery Miles 55 750 Ships in 10 - 15 working days

Soft matter science is nowadays an acronym for an increasingly important class of materials, which ranges from polymers, liquid crystals, colloids up to complex macromolecular assemblies, covering sizes from the nanoscale up the microscale. Computer simulations have proven as an indispensable, if not the most powerful, tool to understand properties of these materials and link theoretical models to experiments. In this first volume of a small series recognized leaders of the field review advanced topics and provide critical insight into the state-of-the-art methods and scientific questions of this lively domain of soft condensed matter research.

Advanced Computer Simulation Approaches for Soft Matter Sciences II (Paperback, Softcover reprint of hardcover 1st ed. 2005):... Advanced Computer Simulation Approaches for Soft Matter Sciences II (Paperback, Softcover reprint of hardcover 1st ed. 2005)
Christian Holm, Kurt Kremer
R5,568 Discovery Miles 55 680 Ships in 10 - 15 working days

This series presents critical reviews of the present and future trends in polymer and biopolymer science including chemistry, physical chemistry, physics and materials science. It is addressed to all scientists at universities and in industry who wish to keep abreast of advances in the topics covered.

Impact Factor Ranking: Always number one in Polymer Science. More information as well as the electronic version of the whole content available at: www.springerlink.com

High-Resolution Transmission Electron Microscopy and Associated Techniques (Hardcover): Peter R Buseck, John M. Cowley, LeRoy... High-Resolution Transmission Electron Microscopy and Associated Techniques (Hardcover)
Peter R Buseck, John M. Cowley, LeRoy Eyring
R2,679 Discovery Miles 26 790 Ships in 12 - 19 working days

This book provides an introduction to the fundamental concepts, techniques, and methods used for electron microscopy at high resolution in space, energy, and even in time. It delineates the theory of elastic scattering, which is most useful for spectroscopies and chemical analyses. There are also discussions of the theory and practice of image calculations, and applications of HRTEM to the study of solid surfaces, highly disordered materials, solid state chemistry, minerology, semiconductors, and metals. Contributors include: J. Cowley, J. Spence, P. Buseck, P. Self, and M.A. O'Keefe. Compiled by experts in the fields of geology, physics and chemistry, this comprehensive text will be the standard reference for years to come.

Laser Experiments for Chemistry and Physics (Paperback): Robert N. Compton, Michael A. Duncan Laser Experiments for Chemistry and Physics (Paperback)
Robert N. Compton, Michael A. Duncan
R1,733 Discovery Miles 17 330 Ships in 12 - 19 working days

Lasers are employed throughout science and technology, in fundamental research, the remote sensing of atmospheric gases or pollutants, communications, medical diagnostics and therapies, and the manufacturing of microelectronic devices. Understanding the principles of their operation, which underlie all of these areas, is essential for a modern scientific education. This text introduces the characteristics and operation of lasers through laboratory experiments designed for the undergraduate curricula in Chemistry and Physics. Introductory chapters describe the properties of light, the history of laser invention, the atomic, molecular and optical principles behind how lasers work, and the kinds of lasers available today. Other chapters include the basic theory of spectroscopy and computational chemistry used to interpret laser experiments. Experiments range from simple in-class demonstrations to more elaborate configurations for advanced students. Each chapter has historical and theoretical background, as well as options suggested for variations on the prescribed experiments. The text will be useful for undergraduates students in advanced lab classes, for instructors designing these classes, or for graduate students beginning a career in laser science.

Active Assessment: Assessing Scientific Inquiry (Paperback, 2009 ed.): David I. Hanauer, Graham F. Hatfull, Debbie Jacobs-Sera Active Assessment: Assessing Scientific Inquiry (Paperback, 2009 ed.)
David I. Hanauer, Graham F. Hatfull, Debbie Jacobs-Sera
R1,486 Discovery Miles 14 860 Ships in 10 - 15 working days

The term scienti?c inquiry as manifest in different educational settings covers a wide range of diverse activities. The differences in types of scienti?c inquiry can be organized along a continuum according to the degree of teacher control and intellectual sophistication involved in each type of inquiry. Types of scienti?c inquiry can also be de?ned according to whether they produce cultural knowledge or personal knowledge. Authentic scienti?c inquiry is de?ned according to ?ve characteristics: devel- ment of personal and cultural knowledge; contextualized scienti?c knowledge; the progression toward high-order problem solving; social interaction for s- enti?c goals; and scienti?c inquiry as a multi-stage and multi-representational process. The de?nition of scienti?c inquiry that forms the basis for the development of an assessment program consists of a two-part analytical frame: the de?nition of knowledge types relevant to scienti?c inquiry and the de?nition of an organi- tional frame for these knowledge types. Four types of knowledge are signi?cant for the de?nition of a speci?c s- enti?c inquiry program: cognitive knowledge, physical knowledge, represen- tional knowledge, and presentational knowledge. All four of these knowledge types are considered signi?cant. These four types of knowledge are organized in a framework that consists of two intersecting axes: the axis of knowledge types and the axis of stages of a s- ci?c scienti?c inquiry. This framework describes scienti?c inquiry as multi-stage process that involves the development of a series of in-lab outcomes (represen- tions) over an extended period of time.

Molecular Biomethods Handbook (Hardcover, 2nd ed. 2008): John M. Walker, Ralph Rapley Molecular Biomethods Handbook (Hardcover, 2nd ed. 2008)
John M. Walker, Ralph Rapley
R5,941 Discovery Miles 59 410 Ships in 10 - 15 working days

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors own research programs.

Electron Microscopy of Polymers (Hardcover, 2008 ed.): Goerg H. Michler Electron Microscopy of Polymers (Hardcover, 2008 ed.)
Goerg H. Michler
R2,976 Discovery Miles 29 760 Ships in 10 - 15 working days

The study of polymers by electron microscopy (EM) needs special techniques, precautions and preparation methods, including ultramicrotomy. General characteristics of the different techniques of EM, including scanning force microscopy, are given in this hands-on book. The application of these techniques to the study of morphology and properties, particularly micromechanical properties, is described in detail. Examples from all classes of polymers are presented.

Lungenfunktion und Spiroergometrie - Interpretation und Befunderstellung unter Einschluss der arteriellen Blutgasanalyse... Lungenfunktion und Spiroergometrie - Interpretation und Befunderstellung unter Einschluss der arteriellen Blutgasanalyse (German, Hardcover, 3. Aufl. 2013)
Paul Haber
R2,869 Discovery Miles 28 690 Ships in 12 - 19 working days

Lungenfunktionsuntersuchung, inklusive der arteriellen Blutgasanalyse, und Spiroergometrie zahlen zu den etablierten Diagnoseverfahren. Der Autor behandelt die physiologischen und physikalischen Grundlagen und vermittelt die Analyse und klinische Interpretation der Befunde: Messwerte, typische Befundmuster und ihre Deutung. Fur die 3.Auflage wurden Definitionen und Referenzwerte aktualisiert. Neu sind Themen wie die Anwendung der Wassermann schen Neunfeldertafel. Der Band enthalt die neueste Demonstrationsversion der Software Pulmopret light.

Molecular Biomethods Handbook (Paperback, 2nd ed. 2008): John M. Walker, Ralph Rapley Molecular Biomethods Handbook (Paperback, 2nd ed. 2008)
John M. Walker, Ralph Rapley
R5,902 Discovery Miles 59 020 Ships in 10 - 15 working days

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors own research programs.

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