Electron Energy Loss Spectroscopy (EELS) is a high resolution technique used for the analysis of thin samples of material. The technique is used in many modern transmission electron microscopes to characterise materials. This book provides an up-to-date introduction to the principles and applications of EELS. Specific topics covered include, theory of EELS, elemental quantification, EELS fine structure, EELS imaging and advanced techniques.

An introductory level guide to electron diffraction in the TEM - This book is a practical guide to electron diffraction in the transmission electron microscope (TEM). Case studies and examples are used to provide an invaluable introduction to the subject for those new to the technique. The book explains the basic methods used to obtain diffraction patterns with the TEM. The numerous illustrations aid the understanding of the conclusions reached. * Gives useful case studies and examples to guide the new user * User-friendly approach with simple explanations and informative illustrations Explains the basic methods of how to obtain images and patterns with the TEM, and how to interpret them. TAMs are used in many labs, and allow users to explore the structure of a specimen of interest (e.g. a section through a particular material, crystal or tissue). The TEM is used to pass electrons through a sample, and the electrons are scattered or diffracted in order to produce an image. This book looks at the patterns produced by diffracted electrons to study the appearance, orientation and structure of the sample.

An outstanding practical guide to the most common chemometric methods in use today

Chemometrics explains how to apply the most widely used pattern recognition and multivariate calibration techniques to solve data analysis problems. This practical guide describes all key methods in terms of processes and applications in order to help the reader easily identify the best technique for a given situation.

Drawing on years of industrial experience with chemometric tools, the authors share their six basic steps, or "habits," for achieving reliable chemometric results, and cover key areas such as:

• Defining and understanding the problem
• Experimental planning and design
• Preprocessing of samples and variables
• Supervised and unsupervised pattern recognition
• Classical and inverse methods of multivariate calibration

Complete with helpful chapter-end summaries, technical references, and more, this book is an invaluable hands-on resource for analytical chemists and laboratory scientists who use chemometrics in their work.

Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

The first edition of this classic book remains one of the very few introductory books covering both theoretical and practical aspects of thermal analysis (TA). This new edition includes a much enlarged section on MDSC, in which the instrument is described and a critical appraisal of the technique presented. Other additions include new sections on rate-controlled TGA, OTTER, and Specific Heat Spectroscopy, and a thoroughly updated section on X-Ray DSC. This very practical book is a must for people who use thermal analysis techniques in their everyday work. "An excellent introductory text" — Review of 1st Edition.

With IC technology continuing to advance, the analysis of very small structures remains critically important. Microscopy of Semiconducting Materials provides an overview of advances in semiconductor studies using microscopy. The book explores the use of transmission and scanning electron microscopy, ultrafine electron probes, and EELS to investigate semiconducting structures. It also covers specimen preparation using focused ion beam milling and advances in microscopy techniques using different types of scanning probes, such as AFM, STM, and SCM. In addition, the book discusses a range of materials, from finished devices to partly processed materials and structures, including nanoscale wires and dots.
This volume provides an authoritative reference for all academics and researchers in materials science, electrical and electronic engineering and instrumentation, and condensed matter physics.

Elastic and inelastic scattering in transmission electron microscopy (TEM) are important research subjects. For a long time, I have wished to systematically summarize various dynamic theories associated with quantitative electron micros copy and their applications in simulations of electron diffraction patterns and images. This wish now becomes reality. The aim of this book is to explore the physics in electron diffraction and imaging and related applications for materials characterizations. Particular emphasis is placed on diffraction and imaging of inelastically scattered electrons, which, I believe, have not been discussed exten sively in existing books. This book assumes that readers have some preknowledge of electron microscopy, electron diffraction, and quantum mechanics. I anticipate that this book will be a guide to approaching phenomena observed in electron microscopy from the prospects of diffraction physics. The SI units are employed throughout the book except for angstrom (A), which is used occasionally for convenience. To reduce the number of symbols used, the Fourier transform of a real-space function P'(r), for example, is denoted by the same symbol P'(u) in reciprocal space except that r is replaced by u. Upper and lower limits of an integral in the book are (-co, co) unless otherwise specified. The (-co, co) integral limits are usually omitted in a mathematical expression for simplification. I very much appreciate opportunity of working with Drs. J. M. Cowley and J. C. H. Spence (Arizona State University), J."

Water is the most basic need of mankind. Drinking water is considered the most essential use of water in life. Therefore it must be free of pathogens, toxins and carcinogens.

Absolutley pure water does not exist in nature. Surface water absorbs particles, carbon dioxide and other gases and mixes with silt and inorganic matters from the environment. When treated and untreated domestic and industrial waste is discharged into natural bodies of water the situation becomes even more complex. Thus human waste, drinking water and communicable diseases are directly related.

Water contamination is measured by the level of pollutants present in a sample. Regular analytical estimation of wastewater is the answer. This manual emphasizes the importance of water purity for drinking and domestic purposes, different types of water and their utilization in various activities, the water quality requirements and criteria of International and Governmental Agencies, and simple estimation procedures and the significance of each analytical test.

Quality Assessment of Water and Wastewater describes methods for ascertaining the quality and contamination levels of waters from a range of sources like ground, surface, potable water supplies, marine, beaches, swimming pools and other recreational facilities, and domestic and industrial wastewater. It includes important derivatives used in the preparation of standard solutions, data analysis, interpretation and units of expressions of the results. It also discusses all major pollutants - their origins and impact on the environment and health - with the basic chemistry of their analysis and complete methodology explained systematically.

The central role of RNA in many cellular processes, in biotechnology, and as pharmaceutical agents, has created an interest in experimental methods applied to RNA molecules. This book provides scientists with a comprehensive collection of thoroughly tested up-to-date manuals for investigating RNA-protein complexes "in vitro." The protocols can be performed by researchers trained in standard molecular biological techniques and require a minimum of specialized equipment. The procedures include recommendation of suppliers of reagents.

The central role of RNA in many cellular processes, in biotechnology, and as pharmaceutical agents, has created an interest in experimental methods applied to RNA molecules. This book provides scientists with a comprehensive collection of thoroughly tested up-to-date manuals for investigating RNA-protein complexes "in vitro." The protocols can be performed by researchers trained in standard molecular biological techniques and require a minimum of specialized equipment. The procedures include recommendation of suppliers of reagents.

This volume describes why a microscope is necessary, the interactions of light with matter and the use of the stereo low-power microscope. It examines the high-power compound microscope, detailing both the older stands with separate lamp, as well as the latest research models with integral illumination and infinity-corrected optics.

The new edition of this widely-used sourcebook details the startlingly array of diagnostic equipment available in the medical laboratory of the nineties, and also covers maintenance and quality assurance for each type of instrument. This book includes 17 completely rewritten chapters and 7 new ones, on nephelometry and turbidimetry, gas chromatography, mass spectrometry, flow cytometry, automated immunoassay systems, automated blood bank systems, and physician's office laboratory instrumentation.

Series Information:
Garland Encyclopedias in the History of Science

This practical handbook provides an introduction for all scanning transmission electron microscopy (STEM) users, but with complete background information concerning features and advantages of the technology. The text is without rigorous mathematical treatment of the fundamentals of STEM, yet comprehensive in its qualitative descriptions.

This practical handbook describes the basic principles and applications of confocal laser scanning microscopy. Information is given on the purpose of, and practical requirements for, the different imaging modes. Guidance is also provided on sample preparation and imaging protocols. The limitations and sensitivity of each method are discussed, imaging applications are illustrated and recent developments in the field are described.

This book covers the fundamental principles of fluorescence and their application to fluorescence microscopy, and presents applications to immunofluorescence, in situ hybridization, and photomicrography. It provides troubleshooting guidance to guide the user through commonly encountered problems.

Electron Microscopy and Analysis 1997 celebrates the centenary anniversary of the discovery of the electron by J.J. Thomson in Cambridge and the fiftieth anniversary of this distinguished Institute group. The book includes papers on the early history of electron microscopy (from P. Hawkes), the development of the scanning electron microscope at Cambridge (from K. Smith), electron energy loss spectroscopy (from L.M. Brown), imaging methods (from J. Spence), and the future of electron microscopy (from C. Humphreys). Covering a wide range of applications of advanced techniques, it discusses electron imaging, electron energy-loss and x-ray analysis, and scanning probe and electron beam microscopies. This volume is a handy reference for professionals using microscopes in all areas of physics, materials science, metallurgy, and surface science to gain an overview of developments in our understanding of materials microstructure and of advances in microscope interrogation techniques.

Immunoassay development is a multidisciplinary activity involving a wide range of skills possessed by few laboratories. This presentation of tried and tested methods should enable scientists and researchers in the pharmaceutical and related industries to more rapidly and effectively develop immunoassays upon which their work is becoming heavily dependent.; Important methods are included for preparing Lapten-protein conjugates and raising the necessary antibodies, concentrating on polyclonal sera, as well as methods for the synthesis of radio and enzyme labelled tracers. Particular attention is paid to the requirements of the regulatory authorities such as the FDA (Food and Drug Administration) with respect to assay validation. Further chapters deal with assay development and optimization, curve fitting and quality control procedures.

This text describes the interactions of light and the specimen in the microscope in relation to the manner in which they are used to generate contrast in the image of a specimen. Chapters cover bright-field and dark-ground techniques for both transmitted and reflected light, as well as the effects of the refractive index of the mounting medium surrounding the specimen. Subsequent chapters deal with the use of polarized light, phase and modulation contrast, interference and fluorescence and contrast technique outside the microscope.

This work provides detailed practical methods for specimen preparation for negative staining and cryoelectron microscopy of thinly spread samples of biological particulates. The different possibilities are emphasized and the benefits to be gained by the various techniques clearly explained. A wide range of biological applications are presented.

Through its clear presentation of the basic concepts, this book quickly enables the reader to understand front-line research papers. It describes the principles of the electrophoresis of nucleic acids through agarose and polyacrylamide gels without resort to complicated protocols and recipes.

Spectroscopy and spectrofluorometry are core techniques used throughout biology and medicine. These techniques evolve continuously and this book provides information on the latest advances in spectroscopic methods. Light Spectroscopy begins by describing the basic principles and then provides practical guidance on the wide range of current techniques, their application, and analysis of the results obtained.

The First Book on CRS Microscopy Compared to conventional Raman microscopy, coherent Raman scattering (CRS) allows label-free imaging of living cells and tissues at video rate by enhancing the weak Raman signal through nonlinear excitation. Edited by pioneers in the field and with contributions from a distinguished team of experts, Coherent Raman Scattering Microscopy explains how CRS can be used to obtain a point-by-point chemical map of live cells and tissues. In color throughout, the book starts by establishing the foundation of CRS microscopy. It discusses the principles of nonlinear optical spectroscopy, particularly coherent Raman spectroscopy, and presents the theories of contrast mechanisms pertinent to CRS microscopy. The text then provides important technical aspects of CRS microscopy, including microscope construction, detection schemes, and data analyses. It concludes with a survey of applications that demonstrate how CRS microscopy has become a valuable tool in biomedicine. Due to its label-free, noninvasive examinations of living cells and organisms, CRS microscopy has opened up exciting prospects in biology and medicine-from the mapping of 3D distributions of small drug molecules to identifying tumors in tissues. An in-depth exploration of the theories, technology, and applications, this book shows how CRS microscopy has impacted human health and will deepen our understanding of life processes in the future.

The contents of the letters published here, again show the great range of subjects that occupied Van Leeuwenhoek: from sugar candy, the shape and crystal structure of diamonds, the dissolution of silver crystals in aqua fortis to gold dust from Guinea dissolved in aqua regia and the dissolution and separation of gold, silver, and copper.

Every volume in the Series contains the texts in the original Dutch and an English translation. The great range of subjects studied by Van Leeuwenhoek is reflected in these letters: instruments to measure water, pulmonary diseases; experiments relating to the solution of gold and silver; salt crystals and grains of sand; botanical work, such as duckweed and germination of orange pips; description on protozoa. blood, spermatozoa and health and hygiene, for example and harmfulness of tea and coffee and the benefits of cleaning teeth.

Introducing students to basic lab techniques and illustrating core chemical principles Prepared by John H. Nelson and Kenneth C. Kemp, both of the University of Nevada, this manual contains 43 finely tuned experiments chosen to introduce students to basic lab techniques and to illustrate core chemical principles. In the 14th Edition, all experiments were carefully edited for accuracy, safety, and cost. Pre-labs and questions were revised and new experiments added concerning solutions, polymers, and hydrates. Each of the experiments is self-contained, with sufficient background material, enabling students to conduct and understand the experiment. Each has a pedagogical objective to exemplify one or more specific principles. Because the experiments are self-contained, they may be undertaken in any order, although the authors have found in their General Chemistry course that the sequence of Experiments 1 through 7 provides the firmest background and introduction. To assist the student, the authors have included pre-lab questions for the student to answer before starting the lab. The questions are designed to help the student understand the experiment, to learn how to do the necessary calculations to treat their data, and as an incentive to read the experiment in advance.