Fabrication technologies for nanostructured devices have been developed recently, and the electrical and optical properties of such nanostructures are a subject of advanced research.This book describes the different approaches to spectroscopic microscopy, i.e., Electron Beam Probe Spectroscopy, Spectroscopic Photoelectron Microscopy, and Scanning Probe Spectroscopy. It will be useful as a compact source of reference for the experienced reseracher, taking into account at the same time the needs of postgraduate students and nonspecialist researchers by using a tutorial approach throughout.

Electron tunnelling spectroscopy is a research tool which has strongly advanced understanding of superconductivity. With the invention of the scanning tunneling microscope, STM, by Nobelists G. Binnig and H. Rohrer, beautiful images of atoms, rings of atoms and of exotic states in high temperature superconductors have appeared. Some of the most famous images of any kind, at this date, are STM topographs. This book explains the physics and the instrumentation behind the advances illustrated in the famous images, and summarizes the state of knowledge that has resulted. It presents the current state of the art of tunneling- and scanning tunneling spectroscopies of atoms, molecules and especially superconductors. The first edition of Principles of Electron Tunneling Spectroscopy has been a standard reference for active researchers for many years. This second edition fully embraces the advances represented by the scanning tunnelling microscope and, especially, scanning tunnelling spectroscopy. Stunning images of single atoms and spectral images of impurity states in high temperature superconductors will set this volume apart from its predecessor. The background and current status are provided for applications of Scanning Tunneling Microscopy and Spectroscopy to single atoms and molecules, including determination of bonding energies and vibrational frequencies. The applications to high temperature superconductivity are carefully introduced and the current status is described. A new section covers the astounding advances in instrumentation, which now routinely provide atomic resolution, and, in addition, developments in imaging and image processing, such as Fourier Transform Scanning Tunneling Spectroscopy.

Neutrons are extremely versatile probes for investigating structure and dynamics in condensed matter. Due to their large penetration depth, they are ideal for in-situ measurements of samples situated in sophisticated and advanced environments. The advent of new high-intensity neutron sources and instruments, as well as the development of new real-time techniques, allows the tracking of transformation processes in condensed matter on a microscopic scale. The present volume provides a review of the state of the art of this new and exciting field of kinetics with neutrons.

Algae are simple, primitive, heterogeneous, autotrophic, eukaryotic or prokaryotic organisms that lead a symbiotic, parasitic or free-living mode of life. Microalgae and macroalgae possess great potential in various fields of application. Microalgae are ubiquitous and extremely diverse microorganisms that can accumulate toxic contaminants and heavy metals from wastewater, making them a superior candidate to become a powerful nanofactory. Algae were discovered to reduce the presence of metal ions, and afterwards aid in the biosynthesis of nanoparticles. Since algae-mediated biogenic nanoparticles are eco-friendly, cost-effective, high-yielding, speedy and energy-efficient, a large number of studies have been published on them in the last few years. This book focuses on recent progress on the utilization of algae for the synthesis of nanoparticles, their characterization and the possible mechanisms involved. Bioprospecting Algae for Nanosized Materials describes the synthesis of algal nanomaterials and its application in various fields for sustainable development. This book outlines the procedures to prepare phyconanomaterials, techniques to utilize the nanomaterials, and applications in agriculture, environment and medicine.

For some people with disabilities, their interest and skills are best applied to laboratory work. Science laboratories are environments where hazardous materials and processes are in use, and assessments are required to mitigate risk and ensure compliance with Occupational Safety and Health Administration (OSHA) and Environmental Protection Agency (EPA) regulations. Accommodating individuals in a laboratory requires balancing adherence to those regulations, as well as the Americans with Disabilities Act (ADA) technical access standards. Individualized assessment and accommodation are needed to ensure that a qualified individual with a disability can work or study effectively in the laboratory while ensuring a safe working environment for all. This book is intended to be a helpful guide for professionals to understand how to provide equal access to people with disabilities in a laboratory environment. It will review the breadth of protections that are provided by the ADA. This book also covers the roles and responsibilities of persons involved in laboratory oversight, including institutional policies and their limitations with respect to providing appropriate support for individualized assessments in the laboratory.

This book discusses fundamentally new biomedical imaging methods, such as holography, holographic and resonant interferometry, and speckle optics. It focuses on the development of holographic interference microscopy and its use in the study of phase objects such as nerve and muscle fibers subjected to the influence of laser radiation, magnetic fields, and hyperbaric conditions. The book shows how the myelin sheath and even the axon itself exhibit waveguide properties, enabling a fresh new look at the mechanisms of information transmission in the human body. The book presents theoretically and experimentally tested holographic and speckle-optical methods and devices used for investigating complex, diffusely scattering surfaces such as skin and muscle tissue. Additionally, it gives broad discussion of the authors' own original fundamental and applied research dedicated to helping physicians introduce new contact-less methods of diagnosis and treatment of diseases of the cardiovascular and neuromuscular systems into medical practice. The book is aimed at a broad spectrum of scientific specialists in the fields of speckle optics, holography, laser physics, morphology and cytochemistry, as well as medical professionals such as physiologists, neuropathologists, neurosurgeons, cardiologists and dentists.

The Nobel Prize of 1986 on Sc- ning Tunneling Microscopy sig- led a new era in imaging. The sc- ning probes emerged as a new i- trument for imaging with a pre- sion suf?cient to delineate single atoms. At ?rst there were two - the Scanning Tunneling Microscope, or STM, and the Atomic Force Mic- scope, or AFM. The STM relies on electrons tunneling between tip and sample whereas the AFM depends on the force acting on the tip when it was placed near the sample. These were quickly followed by the - gneticForceMicroscope,MFM,and the Electrostatic Force Microscope, EFM. The MFM will image a single magnetic bit with features as small as 10nm. With the EFM one can monitor the charge of a single electron. Prof. Paul Hansma at Santa Barbara opened the door even wider when he was able to image biological objects in aqueous environments. At this point the sluice gates were opened and a multitude of different instruments appeared. There are signi?cant differences between the Scanning Probe Microscopes or SPM, and others such as the Scanning Electron Microscope or SEM. The probe microscopes do not require preparation of the sample and they operate in ambient atmosphere, whereas, the SEM must operate in a vacuum environment and the sample must be cross-sectioned to expose the proper surface. However, the SEM can record 3D image and movies, features that are not available with the scanning probes.

In Laser Physics the interaction of radiation and matter, and the principles of laser operation are treated at a level suitable for fourth-year undergraduate courses or introductory graduate courses in physics, chemistry or engineering. The factors which determine efficiency, wavelength coverage, output power, and beam quality of the different classes of laser are treated both in terms of fundamental theory and practical construction aspects. Details of established types of solid-state, semiconductor, and gas lasers are examined together with the techniques that enable their output to be converted widely across the spectrum. The latest advances in high power fibre lasers, femtosecond lasers, and X-ray lasers are explained. The text is liberally illustrated with more than 300 diagrams. An extensive bibliography is provided, together with numerical problems in each chapter. Solutions are available via the web.
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Acoustic microscopy enables the elastic properties of materials to be imaged and measured with the resolution of a good microscope. By using frequencies in the microwave regime, it is possible to make the acoustic wavelength comparable with the wavelength of light, and hence to achieve a resolution comparable with an optical microscope. Solids can support both longitudinal and transverse acoustic waves. At surfaces a unique combination of the two known as Raleigh waves can propagate, and in many circumstances these dominate the contrast in acoustic microscopy. Following the invention of scanning probe microscopes, it is now possible to use an atomic force microscope to detect the acoustic vibration of a surface with resolution in the nanometre range, thus beating the diffraction limit by operating in the extreme near-field. This second edition of Acoustic Microscopy has a major new chapter on the technique and applications of acoustically excited probe microscopy.

Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.

Mammalian cells have evolved a complex multicomponent machinery that enables them to sense and respond to a wide variety of potentially toxic agents present in their environment. These stress responses are often associated with an increased cellular capacity to tolerate normally lethal levels of an insult. The realization that the mammalian stress response may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer, has led to an explosion of interest in this research area. Stress Response: Methods and Protocols brings together a diverse array of practical methodologies that may be employed to address various aspects of the response of mammalian cells to environmental stress. The p- tocols are carefully described by authors who have both devised and succe- fully employed them, and they represent a mixture not only of well-established techniques, but also new technologies at the leading edge of research. The areas covered include the detection and assay of stress-induced damage, the acti- tion of signal transduction pathways, stress-inducible gene expression, and stress protein function. Although no volume of this size can be comprehensive and the topics covered reflect a personal choice, it is hoped that it will prove of subst- tial interest and use to a wide range of research workers in the field.

The past three decades have witnessed the great success of lithium-ion batteries, especially in the areas of 3C products, electrical vehicles, and smart grid applications. However, further optimization of the energy/power density, coulombic efficiency, cycle life, charge speed, and environmental adaptability are still needed. To address these issues, a thorough understanding of the reaction inside a battery or dynamic evolution of each component is required. Microscopy and Microanalysis for Lithium-Ion Batteries discusses advanced analytical techniques that offer the capability of resolving the structure and chemistry at an atomic resolution to further drive lithium-ion battery research and development. * Provides comprehensive techniques that probe the fundamentals of Li-ion batteries. * Covers the basic principles of the techniques involved as well as its application in battery research. * Describes details of experimental set-ups and procedure for successful experiments. This reference is aimed at researchers, engineers, and scientists studying lithium-ion batteries including chemical, materials, and electrical engineers, as well as chemists and physicists.

This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.

A superb collection of time-tested, key techniques for investigating nervous system function, as well as the actions of drugs on the nervous system. The methods range from authoritative treatment of such well-established techniques as recording electrical activity in the brain, to practical coverage of state-of-the-art methods, including neural transplantation and antisense technology. The techniques described are particularly suitable for studying the mechanisms of action of psychotropic drugs and drugs of abuse, as well as the etiology and pharmacotherapy of neuropsychiatric diseases and neurodegenerative disorders. Several of the methods detailed are highly useful in screening potential drugs for the treatment of depression, anxiety disorders, schizophrenia, and Parkinson's and Alzheimer's disease.

Microcharacterization of materials is a rapidly advancing field. Among the many electron and ion probe techniques, the cathodoluminescence mode of an electron probe instrument has reached a certain maturity, which is reflected by an increas ing number of publications in this field. The rapid rate of progress in applications of cathodoluminescence techniques in characterizing inorganic solids has been especially noticeable in recent years. The main purpose of the book is to outline the applications of cath odoluminescence techniques in the assessment of optical and electronic proper ties of inorganic solids, such as semiconductors, phosphors, ceramics, and min erals. The assessment provides, for example, information on impurity levels derived from cathodoluminescence spectroscopy, analysis of dopant concentra tions at a level that, in some cases, is several orders of magnitude lower than that attainable by x-ray microanalysis, the mapping of defects, and the determination of carrier lifetimes and the charge carrier capture cross sections of impurities. In order to make the book self-contained, some basic concepts of solid-state phys ics, as well as various cathodoluminescence techniques and the processes leading to luminescence phenomena in inorganic solids, are also described. We hope that this book will be useful to both scientists and graduate students interested in microcharacterization of inorganic solids. This book, however, was not intended as a definitive account of cathodoluminescence analysis of in organic solids. In considering the results presented here, readers should re member that many materials have properties that vary widely as a function of preparation conditions."

Analytical techniques are powerful tools in a chemist's armoury and this book identifies some of the most important chemical techniques currently in use, along with their applications. Aimed at those with some familiarity with modern chemical techniques, as well as those completely new to them, the book covers much of the basic theory without emphasising the mathematics and physics involved. Where appropriate, descriptions of the instrumentation and sample preparation are included, as are problems with example solutions. More advanced ideas are presented in highlighted boxes, so the novice can happily skip these if desired. Modern Chemical Techniques is based on a series of 'hands-on' symposia that enabled individuals to update their chemical skills and learn about the newest methods, techniques, and instrumentation available. The resource material presented at the symposia is published here, developed and extended into an accessible, illustrated book, making the valuable information it contained available to a much wider audience.

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation

This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization.

The book is concerned with the theory, background, and practical use of transmission electron microscopes with lens correctors that can correct the effects of spherical aberration. The book also covers a comparison with aberration correction in the TEM and applications of analytical aberration corrected STEM in materials science and biology. This book is essential for microscopists involved in nanoscale and materials microanalysis especially those using scanning transmission electron microscopy, and related analytical techniques such as electron diffraction x-ray spectrometry (EDXS) and electron energy loss spectroscopy (EELS).

Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.

This book provides a comprehensive introduction to the methods and variety of Kelvin probe force microscopy, including technical details. It also offers an overview of the recent developments and numerous applications, ranging from semiconductor materials, nanostructures and devices to sub-molecular and atomic scale electrostatics. In the last 25 years, Kelvin probe force microscopy has developed from a specialized technique applied by a few scanning probe microscopy experts into a tool used by numerous research and development groups around the globe. This sequel to the editors' previous volume "Kelvin Probe Force Microscopy: Measuring and Compensating Electrostatic Forces," presents new and complementary topics. It is intended for a broad readership, from undergraduate students to lab technicians and scanning probe microscopy experts who are new to the field.