We are now accustomed to conceive of science as an instrumental activity, producing numbers, measurements and graphs by means of sophisticated devices. This book investigates the historical process that gave rise to this instrumental culture. The contributors trace the displacement of instruments across the globe, the spread of practices or precision and the circulation and appropriation of skills and knowledge.
Through comparative and contextual approaches, the volume confronts the tension between the local and the global, examining the process of the universalization of science. Bringing together case studies ranging from the seventeenth to the twentieth centuries, contributors discuss French, German and British initiatives, as well as the knowledge and techniques of travellers in countries such as India, Africa, South East Asia and the Americas.
Students and researchers interested in the history of science in both Western and non-Western cultures will find this book a valuable and thought-provoking read.

Laymen often consider modern laboratory research to be based on an endless array of sophisticated technologies whose complex capabilities are as important to the outcome of any project as the inventiveness and creativity of the scientists who employ them. Scientists at times may share this point of view until they are con fronted by unexpected findings that demand new approaches, and they discover that yesterday's "sophisticated tools" are today's "blunt instruments." This experience provides a more sobering view of the current state of our scientific methods. It also serves as an impetus for the further development of technology that prepares us for the next stage of advance. Immunologists were confronted by such a technological crises in the late 1970s when they finally were forced to admit that poly clonal antibodies, although quite sensitive reagents, were not spe cific enough to answer many of the questions then confronting virologists and tumor biologists. The answer to the need for specific ity came with the development of monoclonal antibody technology. In the last ten years there have been considerable advances in monoclonal antibody techniques. Today these reagents are much more versatile than they were initially and can be applied to a broad range of problems. Still, most workers who are using these anti bodies are convinced that their potential is far from exhausted, and that at least in some fields we are currently in the early stages of learning how to use them properly.

This book highlights emerging diffraction studies of strain and dislocation gradients with mesoscale resolution, which is currently a focus of research at laboratories around the world. While ensemble-average diffraction techniques are mature, grain and subgrain level measurements needed to understand real materials are just emerging. In order to understand the diffraction signature of different defects, it is necessary to understand the distortions created by the defects and the corresponding changes in the reciprocal space of the non-ideal crystals.Starting with a review of defect classifications based on their displacement fields, this book then provides connections between different dislocation arrangements, including geometrically necessary and statistically stored dislocations, and other common defects and the corresponding changes in the reciprocal space and diffraction patterns. Subsequent chapters provide an overview of microdiffraction techniques developed during the last decade to extract information about strain and dislocation gradients. X-ray microdiffraction is a particularly exciting application compared with alternative probes of local crystalline structure, orientation and defect density, because it is inherently non-destructive and penetrating.

Introduces both optical microscopy and medical imaging with an emphasis on recurring themes such as resolution and contrast to reinforce understanding. Includes many illustrations and boxed material that give more detailed explanations. Features hands-on activities and experiments. Provides end-of-chapter problems for self-study. Offers supplementary online materials including a solutions manual.

The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. "Light and Video Microscopy" will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation.
* Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope
* Clearly develops all ideas from historical and logical foundations
* Laboratory exercises included to assist the reader with practical applications
* Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope

Traditionally experimentation has been understood as an activity performed within the laboratory, but in the twenty-first century this view is being challenged. Schwarz uses ecological and environmental case studies to show how scientific experiments can transcend the laboratory.

Because of its simplicity of use and quantitative results, Scanning Electrochemical Microscopy (SECM) has become an indispensable tool for the study of surface reactivity. The fast expansion of the SECM field over several years has been fueled by the introduction of new probes, commercially available instrumentation, and new practical applications. Scanning Electrochemical Microscopy, Third Edition offers essential background and in-depth overviews of specific applications in self-contained chapters. The vitality and growing popularity of SECM over the past 30+ years have largely been determined by its versatility and capability to remain useful in the changing scientific and technological environments. New applications reported during the last decade reflect significant current activity in biomedical and energy-related research. This thoroughly updated edition provides up-to-date comprehensive reviews of different aspects of SECM. New chapters by renowned professionals in the field cover recent advances in different areas of SECM including nanoSECM, surface reactions and films, batteries, and fuel cells. Expanded coverage of electrocatalysis and surface interrogation as well as photoelectrochemistry and photoelectrocatalysis are also provided. Useful for a broad range of interdisciplinary research-from biological systems to nanopatterning-this book is invaluable to all interested in learning and applying SECM.

Written by an author with more than 40 years of teaching experience in the field, Experiments in Pharmaceutical Chemistry, Second Edition responds to a critical classroom need for material on directed laboratory investigations in biological and pharmaceutical chemistry. This new edition supplies 75 experiments, expanding the range of topics to 22 major areas of pharmaceutical chemistry. These include biochemical groups, botanical classes important to pharmacy, and major drug classifications:

• Carbohydrates
• Lipids
• Proteins
• Enzymes
• Inorganics
• Vitamins
• Steroids
• Plant Acids
• Flavonoids
• Alkaloids
• Tannins
• Resins
• Glycosides
• Gums
• Balsams
• Volatile Oils
• Analgesics
• Anesthetics
• Sulfa Drugs (Sulfonamides)
• Psychotropic Drugs
• Antibiotics
• Nucleic Acids

Sections contain introductions to basic concepts underlying the fields addressed and a specific bibliography relating to each field. Each experiment provides detailed instructions in a user-friendly format, and can be carried out, in most cases, without the need for expensive instrumentation. This comprehensive laboratory manual offers much-needed instructional material for teaching laboratory classes in pharmaceutical chemistry. The breadth of subject matter covered provides a variety of choices for structuring a laboratory course.

Science Sifting is designed primarily as a textbook for students interested in research and as a general reference book for existing career scientists. The aim of this book is to help budding scientists broaden their capacities to access and use information from diverse sources to the benefit of their research careers.The book describes why the capacity to access and integrate both linear and nonlinear information has been an important historic feature of pivotal scientific breakthroughs. Yet, it is a process that our students are rarely, if ever, taught in universities. This book goes beyond simply describing the features of great scientific breakthroughs. It discusses the basis for accessing and using nonlinear information in the linear research context. It also provides a series of tools and exercises that can be used to enhance access to nonlinear information for application to research and other endeavors.Topics covered include focal points in scientific breakthroughs, the use of concepts maps in research, use of different vantage points, information as patterns, fractals for the scientist, memory storage and access points, and synchronicities. Young researchers need useful tools to help with a more holistic approach to their research careers. This book provides the useful tools to support flexibility and creativity across a long-term research career.Roald Hoffmann - Winner of the 1981 Nobel Prize in Chemistry - has contributed the to Science Sifting. More information on Professor Hoffmann can be found at .

Science Sifting is designed primarily as a textbook for students interested in research and as a general reference book for existing career scientists. The aim of this book is to help budding scientists broaden their capacities to access and use information from diverse sources to the benefit of their research careers.The book describes why the capacity to access and integrate both linear and nonlinear information has been an important historic feature of pivotal scientific breakthroughs. Yet, it is a process that our students are rarely, if ever, taught in universities. This book goes beyond simply describing the features of great scientific breakthroughs. It discusses the basis for accessing and using nonlinear information in the linear research context. It also provides a series of tools and exercises that can be used to enhance access to nonlinear information for application to research and other endeavors.Topics covered include focal points in scientific breakthroughs, the use of concepts maps in research, use of different vantage points, information as patterns, fractals for the scientist, memory storage and access points, and synchronicities. Young researchers need useful tools to help with a more holistic approach to their research careers. This book provides the useful tools to support flexibility and creativity across a long-term research career.Roald Hoffmann - Winner of the 1981 Nobel Prize in Chemistry - has contributed the to Science Sifting. More information on Professor Hoffmann can be found at .

Advan ces in production technology.- System analysis of yield trials can raise efficiency of breeding for yield.- Research needs in the production of high quality seeds.- Breeding for quality and resistance to fusarial wilt in gladiolus.- Reproductive biology of potato: Basis for true seed production.- Resistance breeding under coordinated programme.- Quality improvement of Punjab grapes.- Exploitation of allien genes for yellow vein mosaic resistance in okra.- Use of vesicular-arbuscular mycorrhiza (VAM) as biofertilizer for horticultural plants in developing countries.- Foliar feeding of zinc and iron in peach.- Conservation of wild plants of horticultural importance from Tumkur district, Karnataka.- Potential of seed storage at ambient temperature using organic liquids to avoid refrigeration.- Chemical weeding as a labour saving device in management of fruit nurseries.- Role of germplasm in Citrus rootstock improvement.- Comparative efficacy of single and split applied nitrogen on yield and quality of 'Perlette' grapes.- Induced mutations recovered in M2 and subsequent generations in three varieties of okra (Abelmoschus esculentus (L.) Moench).- Plant genetic manipulation.- Role of viruses in plant biotechnology.- Microbial populations, host-specific virulence & plant resistance genes.- Genetically engineered resistance in plants against viral infection.- Genetic engineering and transformation of monocots for crop improvement.- Agrobacterium-mediated gene transfer in Citrus reticulata Blanco.- Regeneration and genetic transformation studies in watermelon (Citrullus vulgaris L. cv. Melitopolski).- Molecular aspects of cytokinin's stymied action on auxin-mediated new root formation in the hypocotyls of Phaseolus vulgaris L..- Plant cell and tissue culture.- Commercial aspects of micropropagation.- Large scale micropropagation: it's strategic use in horticulture.- Micropropagation of bulb crops.- Current status of forest biotechnology.- Large scale secondary metabolite production: the sanguinarine story.- Current research on micropropagation of Sulcorebutia alba Rausch, Syringa vulgaris L., Hip-peastrum hybrids and Tulipa hybrids.- Plant cell culture and food biotechnology: current trends and future prospects.- Tissue culture propagation: problems and potentials.- Micropropagation and plant conformity in Anthurium andreanum.- Rapid in vitro propagation of virus-indexed Freesia.- Effect of culture media and growth regulators on in vitro propagation of rose.- Shoot tip culture method for rapid clonal propagation of pomegranate (Punica granatum L.).- Tissue culture strategies for banana.- Gynogenic plants from ovary cultures of Mulberry (Morus indica).- Induction of high frequency somatic embryogenesis and plant regeneration in mandarins.- Tissue culture of medicinal plants: morphogenesis, direct regeneration and somatic embryogenesis.- Production of important phytochemicals through plant cell cultures and scale-up operations: limitations and prospects.- Establishment of somatic cell culture and plant regeneration in grapes (Vitis vinifera L.).- In-ovulo embryo culture in seedless grapes (Vitis vinifera L.).- Micropropagation of Davana (Artemisia pallens Wall.) by tissue culture.- Clonal propagation of bamboo, coffee and mimosa.- Greenhouse technology.- Greenhouse environmental control for Indian conditions.- New seed-grown cut flowers for floriculture industry.- Cut flowers in the Western Hemisphere.- Decision support for integrated greenhouse production systems.- Greenhouse pot plant production in the United States.- Robotics applications to transplanting of plug seedlings.- Plant health and crop protection.- Immunological and molecular approaches to the diagnosis of viruses infecting horticultural crops.- Viruses of ornamentals - identification and diagnosis.- Protein-A supplemented immune electron microscopy for diagnosis of potato viruses X, S, Y and leafroll.- Production of virus-tested seed potatoes.- Pesticide deconta

Efficiency and life time of solar cells, energy and power density of the batteries, and costs of the fuel cells alike cannot be improved unless the complex electronic, optoelectronic, and ionic mechanisms underpinning operation of these materials and devices are understood on the nanometer level of individual defects. Only by probing these phenomena locally can we hope to link materials structure and functionality, thus opening pathway for predictive modeling and synthesis. While structures of these materials are now accessible on length scales from macroscopic to atomic, their functionality has remained Terra Incognitae. In this volume, we provide a summary of recent advances in scanning probe microscopy studies of local functionality of energy materials and devices ranging from photovoltaics to batteries, fuel cells, and energy harvesting systems. Recently emergent SPM modes and combined SPM-electron microscopy approaches are also discussed. Contributions by internationally renowned leaders in the field describe the frontiers in this important field.

This book describes for the first time how Monte Carlo modeling methods can be applied to electron microscopy and microanalysis. Computer programs for two basic types of Monte Carlo simulation are developed from physical models of the electron scattering process--a single scattering program capable of high accuracy but requiring long computation times, and a plural scattering program which is less accurate but much more rapid. Optimized for use on personal computers, the programs provide a real time graphical display of the interaction. The programs are then used as the starting point for the development of programs aimed at studying particular effects in the electron microscope, including backscattering, secondary electron production, EBIC and cathodo-luminescence imaging, and X-ray microanalysis. The computer code is given in a fully annotated format so that it may be readily modified for specific problems. Throughout, the author includes numerous examples of how such applications can be used. Students and professionals using electron microscopes will want to read this important addition to the literature.

The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of "Methods in Cell Biology" covers many areas of CLEM, includinga brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in "C. elegans" embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM.
Covers many areas of CLEM by the best international scientists in the fieldIncludes a brief history and overview on CLEM methods"

This dissertation examines the cultural and educational history of central Missouri between 1820 and 1860, and in particular, the issue of master-slave relationships and how they affected education (broadly defined as the transmission of Southern culture). Although Missouri had one of the lowest slave populations during the Antebellum period, Central Missouri - or what became known as Little Dixie - had slave percentages that rivaled many regions and counties of the Deep South. However, slaves and slave owners interacted on a regular basis, which affected cultural transmission in the areas of religion, work, and community. Generally, slave owners in Little Dixie showed a pattern of paternalism in all these areas, but the slaves did not always accept their masters' paternalism, and attempted to forge a life of their own.

Fluctuations in the level and pattern of international trade have a profound effect on the economies of less developed countries. This book explores the relationship between international trade and globl development through a series of essays which relate advances in trade theory to key issues in trade policy. The book, first published in 1991, is in honour of Jagdish Bhagwati and reflects the range and significance of his contributions to international economics.

The Advancing Frontier in the Knowledge of the Structure of Interphases.- Some Recent Spectroscopic Approaches to the Solid-Solution Interface.- Application to Electrocatalysis of EMIRS (Electrochemically Modulated Infrared Reflectance Spectroscopy) and Related Techniques.- Photoacoustic Spectroscopy and the In-Situ Characterization of the Electrochemical Interface.- Raman Spectroscopic Techniques in Interfacial Electrochemistry.- Laser Raman Spectroscopy in Studies of Corrosion and Electrocatalysis.- UV-Visible Reflectance Spectroscopy in Electrochemistry.- Study of Anodic Oxides by UV-Visible Potential-Modulated Reflectance Spectroscopy.- Nonlinear Optical Techniques for Surface Studies.- X-Ray Diffraction at the Electrode-Solution Interface.- X-Ray Reflectivity and Surface Roughness.- Surface Structural Investigations by Electron Diffraction Techniques.- Auger Electron Spectroscopy and the Electrochemical Interface.- Photoelectron Spectroscopy (XPS and UPS) of Electrode Surfaces.- Rutherford Backscattering Spectroscopy of Electrode Surfaces.- Electrochemical Applications of Scanning Tunneling Microscopy.

In 1876 the South Kensington Museum held a major international exhibition of scientific instruments and equipment, both historical and contemporary. Many of the items were retained and eventually formed the basis of important collections now held at the Science Museum, London. This is the 1877 third edition of the exhibition catalogue, which was expanded to include a 'large number of objects' received since the publication of the second edition, and which also included corrections in order to 'afford a complete record of the collection for future reference'. In two volumes and twenty sections comprising over 4,500 entries, the catalogue lists a huge variety of items, ranging from slide rules and telescopes to lighthouse parts and medical equipment. It gives detailed explanations of how they were used, and notes of their ownership and provenance, while the opening pages comprehensively record the contributing individuals and institutions in Britain, Europe and America.

Biomedical photonics is currently one of the fastest growing fields, connecting research in physics, optics, and electrical engineering coupled with medical and biological applications. It allows for the structural and functional analysis of tissues and cells with resolution and contrast unattainable by any other methods. However, the major challenges of many biophotonics techniques are associated with the need to enhance imaging resolution even further to the sub-cellular level as well as translate them for in vivo studies. The tissue optical clearing method uses immersion of tissues into optical clearing agents (OCAs) that reduces the scattering of tissue and makes tissue more transparent and this method has been successfully used ever since. This book is a self-contained introduction to tissue optical clearing, including the basic principles and in vitro biological applications, from in vitro to in vivo tissue optical clearing methods, and combination of tissue optical clearing and various optical imaging for diagnosis. The chapters cover a wide range of issues related to the field of tissue optical clearing: mechanisms of tissue optical clearing in vitro and in vivo; traditional and innovative optical clearing agents; recent achievements in optical clearing of different tissues (including pathological tissues) and blood for optical imaging diagnosis and therapy. This book provides a comprehensive account of the latest research and possibilities of utilising optical clearing as an instrument for improving the diagnostic effectiveness of modern optical diagnostic methods. The book is addressed to biophysicist researchers, graduate students and postdocs of biomedical specialties, as well as biomedical engineers and physicians interested in the development and application of optical methods in medicine. Key features: The first collective reference to collate all known knowledge on this topic Edited by experts in the field with chapter contributions from subject area specialists Brings together the two main approaches in immersion optical clearing into one cohesive book

This book provides an introduction to the fundamental concepts, techniques, and methods used for electron microscopy at high resolution in space, energy, and even in time. It delineates the theory of elastic scattering, which is most useful for spectroscopies and chemical analyses. There are also discussions of the theory and practice of image calculations, and applications of HRTEM to the study of solid surfaces, highly disordered materials, solid state chemistry, minerology, semiconductors, and metals. Contributors include: J. Cowley, J. Spence, P. Buseck, P. Self, and M.A. O'Keefe. Compiled by experts in the fields of geology, physics and chemistry, this comprehensive text will be the standard reference for years to come.

The book presents a clear and comprehensive review of the current status of the holographic microscopy with discussion of the positive and negative features of classical and holographic methods for solving the problem of three-dimesional (3D) imaging of phase microscopic objects. Classical and holographic methods of phase, interference and polarization contrast are discussed. Combination of the developed holographic methods with the methods of digital image processing allowed creating the digital holographic interference microscope (DHIM). The first 3D images of native phase microscopic objects such as blood cells were obtained using the DHIM. The results of DHIM application for study of blood erythrocytes, thin films, micro-crystals are presented.

Fluctuations in the level and pattern of international trade have a profound effect on the economies of less developed countries. This book explores the relationship between international trade and globl development through a series of essays which relate advances in trade theory to key issues in trade policy. The book, first published in 1991, is in honour of Jagdish Bhagwati and reflects the range and significance of his contributions to international economics.

This new volume, Microscopy Applied to Materials Sciences and Life Sciences. focuses on recent theoretical and practical advances in polymers and their blends, composites, and nanocomposites related to their microscopic characterization. It highlights recent accomplishments and trends in the field of polymer nanocomposites and filled polymers related to microstructural characterization. This book gives an insight and better understanding into the development in microscopy as a tool for characterization. The book emphasizes recent research work in the field of microscopy in life sciences and materials sciences mainly related to its synthesis, characterizations, and applications. The book explains the application of microscopic techniques in life sciences and materials sciences, and their applications and state of current research carried out. The book aims to foster a better understanding of the properties of polymer composites by describing new techniques to measure microstructure property relationships and by utilizing techniques and expertise developed in the conventional filled polymer composites. Characterization techniques, particularly microstructural characterization, have proven to be extremely difficult because of the range of length-scales associated with these materials. Topics include: *Instrumentation and Techniques: advances in scanning probe microscopy, SEM, TEM, OM. 3D imaging and tomography, electron diffraction techniques and analytical microscopy, advances in sample preparation techniques in-situ microscopy, correlative microscopy in life and material sciences, low voltage electron microscopy. *Life Sciences: Structure and imaging of biomolecules, live cell imaging, neurobiology, organelles and cellular dynamics, multi-disciplinary approaches for medical and biological sciences, microscopic application in plants, microorganism and environmental science, super resolution microscopy in biological sciences. *Materials Sciences: materials for nanotechnology, metals alloys and inter-metallic, ceramics, composites, minerals and microscopy in cultural heritage, thin films, coatings, surfaces and interfaces, carbon based materials, polymers and soft materials and self-assembled materials, semiconductors and magnetic materials. Polymers and inorganic nanoparticles. The volume will be of significant interest to scientists working on the basic issues surrounding polymers, nanocomposites, and nanoparticulate-filled polymers, as well as those working in industry on applied problems, such as processing. Because of the multidisciplinary nature of this research, the book will be valuable to chemists, materials scientists, physicists, chemical engineers, and processing specialists who are involved and interested in the future frontiers of blends.

Scanning Probe Microscopy (SPM) is the enabling tool for nano(bio)technology, which has opened new vistas in many interdisciplinary research areas. Concomitant with the developments in SPM instrumentation and techniques are new and previously unthought-of opportunities in materials nanofabrication and characterisation. In particular, the developments in addressing and manipulating matter at the level of single atoms or molecules, and studies of biological materials (e.g. live cells, or cell membranes) result in new and exciting discoveries.The rising importance of SPM demands a concise treatment in the form of a book which is accessible to interdisciplinary practitioners. This book highlights recent advances in the field of SPM with sufficient depth and breadth to provide an intellectually stimulating overview of the current state of the art. The book is based on a set of carefully selected original works from renowned contributors on topics that range from atom technology, scanning tunneling spectroscopy of self-assembled nanostructures, SPM probe fabrication, scanning force microscopy applications in biology and materials science down to the single molecule level, novel scanning probe techniques, and nanolithography.The variety of topics underlines the strong interdisciplinary character of SPM related research and the combined expertise of the contributors gives us a unique opportunity to discuss possible future trends in SPM related research. This makes the book not merely a collection of already published material but an enlightening insight into cutting edge research and global SPM research trends.

This volume is dedicated to a description of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, intrumentation, and methods underlying all cryo-EM approaches which allow practictioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.

* Includes time-tested core methods and new innovations applicable to any researcher * Methods included are useful to both established researchers and newcomers to the field * Relevant background and reference information given for procedures can be used as a guide