Since its inception, patch-clamp has continued to be widely considered the gold standard method to record ion channel activity. "Patch-Clamp Methods and Protocols, Second Edition," provides a comprehensive collection of new techniques for the development of automated, high-throughput screening systems for pharmacological evaluation, the use of various patch-clamp configurations together with novel molecular biological and imaging methodologies and enhanced stimulation protocols and perfusion systems. Divided into sections on pharmacology, physiology and biophysics, the chapters cover methods to generate more physiologically relevant conditions for drug application and screening technologies, recently developed applications such as optogenetic stimulation, advances in whole-cell recordings in freely-moving animals and novel technologies to create custom microelectrodes designed for reducing the access resistance and improving the rate of molecular diffusion. Patch-clamp is an indispensable technique for conducting pharmacological, physiological and biophysical research aimed at understanding crucial aspects of cellular and network function. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, "Patch-Clamp Methods and Protocols, Second Edition" will provide a useful technical and methodological guide to diverse audiences of electrophysiologists, from students to experienced investigators.

A zebrafish, the hull of a miniature ship, a mathematical equation and a food chain - what do these things have in common? They are examples of models used by scientists to isolate and study particular aspects of the world around us. This book begins by introducing the concept of a scientific model from an intuitive perspective, drawing parallels to mental models and artistic representations. It then recounts the history of modelling from the 16th century up until the present day. The iterative process of model building is described and discussed in the context of complex models with high predictive accuracy versus simpler models that provide more of a conceptual understanding. To illustrate the diversity of opinions within the scientific community, we also present the results of an interview study, in which ten scientists from different disciplines describe their views on modelling and how models feature in their work. Lastly, it includes a number of worked examples that span different modelling approaches and techniques. It provides a comprehensive introduction to scientific models and shows how models are constructed and used in modern science. It also addresses the approach to, and the culture surrounding modelling in different scientific disciplines. It serves as an inspiration for model building and also facilitates interdisciplinary collaborations by showing how models are used in different scientific fields. The book is aimed primarily at students in the sciences and engineering, as well as students at teacher training colleges but will also appeal to interested readers wanting to get an overview of scientific modelling in general and different modelling approaches in particular.

This book presents the latest developments in noncontact atomic force microscopy. It deals with the following outstanding functions and applications that have been obtained with atomic resolution after the publication of volume 2: (1) Pauli repulsive force imaging of molecular structure, (2) Applications of force spectroscopy and force mapping with atomic resolution, (3) Applications of tuning forks, (4) Applications of atomic/molecular manipulation, (5) Applications of magnetic exchange force microscopy, (6) Applications of atomic and molecular imaging in liquids, (7) Applications of combined AFM/STM with atomic resolution, and (8) New technologies in dynamic force microscopy. These results and technologies are now expanding the capacity of the NC-AFM with imaging functions on an atomic scale toward making them characterization and manipulation tools of individual atoms/molecules and nanostructures, with outstanding capability at the level of molecular, atomic, and subatomic resolution. Since the publication of vol. 2 of the book Noncontact Atomic Force Microscopy in 2009 the noncontact atomic force microscope, which can image even insulators with atomic resolution, has achieved remarkable progress. The NC-AFM is now becoming crucial for nanoscience and nanotechnology.

Spark scientific curiosity from a young age with this six-level course through an enquiry-based approach and active learning. Collins International Primary Science fully meets the requirements of the Cambridge Primary Science Curriculum Framework from 2020 and has been carefully developed for a range of international contexts. The course is organised into four main strands: Biology, Chemistry, Physics and Earth and Space and the skills detailed under the 'Thinking and Working Scientifically' strand are introduced and taught in the context of those areas. For each Workbook at Stages 1 to 6, we offer: A write-in Workbook linked to the Student's Book New language development activities help build science vocabulary Earth and Space content covers the new curriculum framework Thinking and Working Scientifically deepens and enhances the delivery of Science skills Actively learn through practical activities that don't require specialist equipment or labs Scaffolding allows students of varying abilities to work with common content and meet learning objectives Supports Cambridge Global Perspectives (TM) with activities that develop and practise key skills Provides learner support as part of a set of resources for the Cambridge Primary Science curriculum framework (0097) from 2020 This series is endorsed by Cambridge Assessment International Education to support the new curriculum framework 0097 from 2020.

This thesis describes novel approaches and implementation of high-resolution microscopy in the extreme ultraviolet light regime. Using coherent ultrafast laser-generated short wavelength radiation for illuminating samples allows imaging beyond the resolution of visible-light microscopes. Michael Zurch gives a comprehensive overview of the fundamentals and techniques involved, starting from the laser-based frequency conversion scheme and its technical implementation as well as general considerations of diffraction-based imaging at nanoscopic spatial resolution. Experiments on digital in-line holography and coherent diffraction imaging of artificial and biologic specimens are demonstrated and discussed in this book. In the field of biologic imaging, a novel award-winning cell classification scheme and its first experimental application for identifying breast cancer cells are introduced. Finally, this book presents a newly developed technique of generating structured illumination by means of so-called optical vortex beams in the extreme ultraviolet regime and proposes its general usability for super-resolution imaging.

"Fluorescence Microscopy: Super-Resolution and other Novel Techniques" delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science. With contributions selected for clarity, utility, and reproducibility, the work provides practical tools for investigating these ground-breaking developments. Emphasizing super-resolution techniques, light sheet microscopy, sample preparation, new labels, and analysis techniques, this work keeps pace with the innovative technical advances that are increasingly vital to biological and biomedical researchers.

With its extensive graphics, inter-method comparisons, and tricks and approaches not revealed in primary publications, "Fluorescence Microscopy" encourages readers to both understand these methods, and to adapt them to other systems. It also offers instruction on the best visualization to derive quantitative information about cell biological structure and function, delivering crucial guidance on best practices in related laboratory research.
Presents a timely and comprehensive review of novel techniques in fluorescence imaging as applied to biological and biomedical researchOffers insight into common challenges in implementing techniques, as well as effective solutions Provides video accompaniment for certain techniques on a companion website for a multi-dimensional perspective: http: //booksite.elsevier.com/9780124095137

The electric dipole moment (EDM) challenge measures a non-zero proton EDM value and this book suggests how the challenge can be met. Any measurably large proton EDM would violate the standard model. The method to be employed uses an intense beam of 'frozen spin' protons circulating for hour-long times in a storage ring 'trap'. The smallness of EDMs allows them to test existing theories, but also makes them hard to measure. Such EDM experiments are inexpensive, at least compared to building accelerators of ever-greater energy.

This volume presents current advanced technologies and methods used in super-resolution microscopy. The chapters in this book cover a wide range of topics such as introducing super-resolution microscopy into a core facility; two-photon STED microscopy for nanoscale imaging of neural morphology in vivo; correlative SIM-STORM microscopy; two-color single-molecule tracking in live cells; and correlative single molecule localization microscopy and confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Super-Resolution Microscopy: Methods and Protocols is a valuable resource for both established and novel researchers and users in this field.

The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe's theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.
* Expands coverage to include discussion of confocal microscopy not found in the previous edition
* Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods

Biobanking, an emerging field supported by academia, industry and health administrators alike, is distinctly different today from the practice that once defined it. The science of Biobanking, which initially involved simply storing blood or tissue samples in a freezer, is now a highly sophisticated field of research, and expected to grow exponentially over the next decade or two. This book aims to serve the purpose of further enriching the available literature on Biobanking, by offering unique and more useful collection of ideas for the future. The book outlines the experiences of developing modern Biobanking repositories in different countries, whilst covering specific topics regarding the many aspects of Biobanking. This book will be of interest to a wide range of readers including: academics, students, volunteers and advocates of patients' rights.

Specialist Periodical Reports provide systematic and detailed review coverage of progress in the major areas of chemical research. Written by experts in their specialist fields the series creates a unique service for the active research chemist, supplying regular critical in-depth accounts of progress in particular areas of chemistry. For over 80 years the Royal Society of Chemistry and its predecessor, the Chemical Society, have been publishing reports charting developments in chemistry, which originally took the form of Annual Reports. However, by 1967 the whole spectrum of chemistry could no longer be contained within one volume and the series Specialist Periodical Reports was born. The Annual Reports themselves still existed but were divided into two, and subsequently three, volumes covering Inorganic, Organic and Physical Chemistry. For more general coverage of the highlights in chemistry they remain a 'must'. Since that time the SPR series has altered according to the fluctuating degree of activity in various fields of chemistry. Some titles have remained unchanged, while others have altered their emphasis along with their titles; some have been combined under a new name whereas others have had to be discontinued. The current list of Specialist Periodical Reports can be seen on the inside flap of this volume.

Advan ces in production technology.- System analysis of yield trials can raise efficiency of breeding for yield.- Research needs in the production of high quality seeds.- Breeding for quality and resistance to fusarial wilt in gladiolus.- Reproductive biology of potato: Basis for true seed production.- Resistance breeding under coordinated programme.- Quality improvement of Punjab grapes.- Exploitation of allien genes for yellow vein mosaic resistance in okra.- Use of vesicular-arbuscular mycorrhiza (VAM) as biofertilizer for horticultural plants in developing countries.- Foliar feeding of zinc and iron in peach.- Conservation of wild plants of horticultural importance from Tumkur district, Karnataka.- Potential of seed storage at ambient temperature using organic liquids to avoid refrigeration.- Chemical weeding as a labour saving device in management of fruit nurseries.- Role of germplasm in Citrus rootstock improvement.- Comparative efficacy of single and split applied nitrogen on yield and quality of 'Perlette' grapes.- Induced mutations recovered in M2 and subsequent generations in three varieties of okra (Abelmoschus esculentus (L.) Moench).- Plant genetic manipulation.- Role of viruses in plant biotechnology.- Microbial populations, host-specific virulence & plant resistance genes.- Genetically engineered resistance in plants against viral infection.- Genetic engineering and transformation of monocots for crop improvement.- Agrobacterium-mediated gene transfer in Citrus reticulata Blanco.- Regeneration and genetic transformation studies in watermelon (Citrullus vulgaris L. cv. Melitopolski).- Molecular aspects of cytokinin's stymied action on auxin-mediated new root formation in the hypocotyls of Phaseolus vulgaris L..- Plant cell and tissue culture.- Commercial aspects of micropropagation.- Large scale micropropagation: it's strategic use in horticulture.- Micropropagation of bulb crops.- Current status of forest biotechnology.- Large scale secondary metabolite production: the sanguinarine story.- Current research on micropropagation of Sulcorebutia alba Rausch, Syringa vulgaris L., Hip-peastrum hybrids and Tulipa hybrids.- Plant cell culture and food biotechnology: current trends and future prospects.- Tissue culture propagation: problems and potentials.- Micropropagation and plant conformity in Anthurium andreanum.- Rapid in vitro propagation of virus-indexed Freesia.- Effect of culture media and growth regulators on in vitro propagation of rose.- Shoot tip culture method for rapid clonal propagation of pomegranate (Punica granatum L.).- Tissue culture strategies for banana.- Gynogenic plants from ovary cultures of Mulberry (Morus indica).- Induction of high frequency somatic embryogenesis and plant regeneration in mandarins.- Tissue culture of medicinal plants: morphogenesis, direct regeneration and somatic embryogenesis.- Production of important phytochemicals through plant cell cultures and scale-up operations: limitations and prospects.- Establishment of somatic cell culture and plant regeneration in grapes (Vitis vinifera L.).- In-ovulo embryo culture in seedless grapes (Vitis vinifera L.).- Micropropagation of Davana (Artemisia pallens Wall.) by tissue culture.- Clonal propagation of bamboo, coffee and mimosa.- Greenhouse technology.- Greenhouse environmental control for Indian conditions.- New seed-grown cut flowers for floriculture industry.- Cut flowers in the Western Hemisphere.- Decision support for integrated greenhouse production systems.- Greenhouse pot plant production in the United States.- Robotics applications to transplanting of plug seedlings.- Plant health and crop protection.- Immunological and molecular approaches to the diagnosis of viruses infecting horticultural crops.- Viruses of ornamentals - identification and diagnosis.- Protein-A supplemented immune electron microscopy for diagnosis of potato viruses X, S, Y and leafroll.- Production of virus-tested seed potatoes.- Pesticide deconta

Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.

This book highlights the current understanding of materials in the context of new and continuously emerging techniques in the field of electron microscopy. The authors present applications of electron microscopic techniques in characterizing various well-known & new nanomaterials. The applications described include both inorganic nanomaterials as well as organic nanomaterials.

The principles and procedures used to obtain structural measurements of terrestrial vegetation communities are presented in this text/reference. Designed to be the standard work on the topic, it provides a balance between conceptual and practical aspects of measurement procedures and techniques. The four commonly used measurements of vegetation - frequency, cover, density, and biomass - are introduced, along with their associated units. There is also up-to-date coverage of vegetation measurement using remote sensing techniques.

The combination of atomic force microscopy with ultrasonic methods allows the nearfield detection of acoustic signals. The nondestructive characterization and nanoscale quantitative mapping of surface adhesion and stiffness or friction is possible. The aim of this book is to provide a comprehensive review of different scanning probe acoustic techniques, including AFAM, UAFM, SNFUH, UFM, SMM and torsional tapping modes. Basic theoretical explanations are given to understand not only the probe dynamics but also the dynamics of tip surface contacts. Calibration and enhancement are discussed to better define the performance of the techniques, which are also compared with other classical techniques such as nanoindentation or surface acoustic wave. Different application fields are described, including biological surfaces, polymers and thin films.

This book focuses on charged-particle optics and microscopy, as well as their applications in the materials sciences. Presenting a range of cutting-edge theoretical and methodological advances in electron microscopy and microanalysis, and examining their crucial roles in modern materials research, it offers a unique resource for all researchers who work in ultramicroscopy and/or materials research. The book addresses the growing opportunities in this field and introduces readers to the state of the art in charged-particle microscopy techniques. It showcases recent advances in scanning electron microscopy, transmission electron microscopy and helium ion microscopy, including advanced spectroscopy, spherical-corrected microscopy, focused-ion imaging and in-situ microscopy. Covering these and other essential topics, the book is intended to facilitate the development of microscopy techniques, inspire young researchers, and make a valuable contribution to the field.

This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.

Specialist Periodical Reports provide systematic and detailed review coverage of progress in the major areas of chemical research. Written by experts in their specialist fields the series creates a unique service for the active research chemist, supplying regular critical in-depth accounts of progress in particular areas of chemistry. For over 80 years the Royal Society of Chemistry and its predecessor, the Chemical Society, have been publishing reports charting developments in chemistry, which originally took the form of Annual Reports. However, by 1967 the whole spectrum of chemistry could no longer be contained within one volume and the series Specialist Periodical Reports was born. The Annual Reports themselves still existed but were divided into two, and subsequently three, volumes covering Inorganic, Organic and Physical Chemistry. For more general coverage of the highlights in chemistry they remain a 'must'. Since that time the SPR series has altered according to the fluctuating degree of activity in various fields of chemistry. Some titles have remained unchanged, while others have altered their emphasis along with their titles; some have been combined under a new name whereas others have had to be discontinued. The current list of Specialist Periodical Reports can be seen on the inside flap of this volume.

"Annual Reports in Computational Chemistry" is a new periodical providing timely and critical reviews of important topics in computational chemistry as applied to all chemical disciplines. Topics covered include quantum chemistry, molecular mechanics, force fields, chemical education, and applications in academic and industrial settings. Each volume is organized into (thematic) sections with contributions written by experts. Focusing on the most recent literature and advances in the field, each article covers a specific topic of importance to computational chemists. "Annual Reports in Computational Chemistry" is a "must" for researchers and students wishing to stay up-to-date on current developments in computational chemistry.
* Broad coverage of computational chemistry and up-to-date information
* Topics covered include bioinformatics, drug discovery, protein NMR, simulation methodologies, and applications in academic and industrial settings
* Each chapter reviews the most recent literature on a specific topic of interest to computational chemists

Chemical analysis moves from laboratory to places where samples to be analysed are located. This trend is aptly termed "on-site analysis". As the dictum says: "from sample transfer to information transfer". Owing to the ever increasing number of samples to be analysed, preliminary screening and selection of samples seems to be necessary, even in the laboratory. Rapid test methods of chemical analysis can solve both these tasks. This book is devoted to test methods that are widely used in environmental, industrial, clinical, forensic, medical, and other areas allowing a rapid, simple and cost-effective analysis - qualitative, semi-quantitative and quantitative to be performed by trained as well as non-trained personnel. Some general characteristic features of test methods and test systems are described in the book, e.g. definitions, advantages and limitations, chemical and physical principles of operation, procedures and protocols, and methodological aspects. Application of test methods in various areas is extensively overviewed, and the test means and test tools pertinent for solving each concrete analytical task are discussed, e.g. paper strips, indicator powders and tubes, tablets, etc. The most important applications of test methods, evidently, are: testing for inorganic and organic components in water (mostly for purposes of environmental control), monitoring of toxic gases and alcohol vapours, detection of narcotics and explosives, determination of glucose, cholesterol, and other components of medical importance.

This book is about China's ambitions in its most complex and internationally visible space endeavor, namely its human space exploration programme. It provides a comprehensive reflection on Chinas strategic direction and objectives in space, including in particular those set forth in its human spaceflight programme and analyses the key domestic and external factors affecting the country's presumed manned lunar ambitions. The objective of the book is to disentangle the opportunities and challenges Chinas space ambitions are creating for other spacefaring nations and for Europe in particular. It therefore includes an in-depth analysis of possible European postures towards China in space exploration and seeks to stimulate a debate on future space strategies in the broader context of world politics.