Three-Dimensional Electron Microscopy, Volume 152 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters focusing on FIB-SEM of mouse nervous tissue: fast and slow sample preparation, Serial-section electron microscopy using ATUM - Automated Tape collecting Ultra-Microtome, Software for automated acquisition of electron tomography tilt series, Scanning electron tomography of biological samples embedded in plastic, Cryo-STEM tomography for Biology, CryoCARE: Content-aware denoising of cryo-EM images and tomograms using artificial neural networks, Expedited large-volume 3-D SEM workflows for comparative vertebrate microanatomical imaging, and many other interesting topics.

Light and Video Microscopy, Third Edition provides a step-by-step journey through philosophy, psychology and the geometrical and physical optics involved in interpreting images formed by light microscopes. The book addresses the intricacies necessary to set up light microscopes that allow one to visualize transparent specimens and, in the process, quantitatively determine various physico-chemical properties of specimens. This updated edition includes the most recent developments in microscopy, ensuring that it continues to be the most comprehensive, easy-to-use, and informative guide on light microscopy. With its presentation of geometrical optics, it assists the reader in understanding image formation and light movement within the microscope.

Maple is a comprehensive symbolic mathematics application which is well suited for demonstrating physical science topics and solving associated problems. Because Maple is such a rich application, it has a somewhat steep learning curve. Most existing texts concentrate on mathematics; the Maple help facility is too detailed and lacks physical science examples, many Maple-related websites are out of date giving readers information on older Maple versions. This book records the author's journey of discovery; he was familiar with SMath but not with Maple and set out to learn the more advanced application. It leads readers through the basic Maple features with physical science worked examples, giving them a firm base on which to build if more complex features interest them.

This book is the first volume in a two-volume compilation on controlled/living radical polymerization. It combines all important aspects of controlled radical polymerization: from synthetic procedures, to rational selection of reaction components, to understanding of the reaction mechanisms, to materials and applications.
This book is focused on recent progress in the rapidly developing field of controlled/living radical polymerization. It is a sequel to ACS Symposium Series 685, 768, 854, 944, 1023, and 1024. Volume 1100 deals with the mechanistic aspects of controlled radical polymerization and describes the recent advances in the most important techniques, whereas Volume 1101 contains chapters on new materials prepared by controlled radical polymerization as well as applications of these materials.

The confocal microscope is appropriate for imaging cells or the measurement of industrial artefacts. However, junior researchers and instrument users sometimes misuse imaging concepts and metrological characteristics, such as position resolution in industrial metrology and scale resolution in bio-imaging. And, metrological characteristics or influence factors in 3D measurement such as height assessment error caused by 3D coupling effect are so far not yet identified. In this book, the authors outline their practices by the working experiences on standardization and system design. This book assumes little previous knowledge of optics, but rich experience in engineering of industrial measurements, in particular with profile metrology or areal surface topography will be very helpful to understand the theoretical concerns and value of the technological advances. It should be useful for graduate students or researchers as extended reading material, as well as microscope users alongside their handbook.

Your biochemistry lab course is an essential component in training for a career in biochemistry, molecular biology, chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. "Biochemistry Laboratory: Modern Theory and Techniques" covers the theories, techniques, and methodologies practiced in the biochemistry teaching and research lab. Instead of specific experiments, it focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. An extensive range of techniques discussed includes Internet databases, chromatography, spectroscopy, and recombinant DNA techniques such as molecular cloning and PCR. The Second Edition introduces cutting-edge topics such as membrane-based chromatography, adds new exercises and problems throughout, and offers a completely updated Companion Website.

This book explicates the optical controls of antiferromagnetic spins by intense terahertz (THz) electromagnetic waves. The book comprises two key components: (1) the experimental demonstration of the enhancement of a THz magnetic field using a split-ring resonator (SRR) and (2) the control of the direction of magnetization by using the enhanced THz magnetic field to break the symmetry of optically-induced phase transition. These make up the first step leading to future spintronics devices. In the beginning of the book, the author reviews the basics of the ultrafast laser and nonlinear optical techniques as well as the previously achieved experiments to control spin dynamics by THz magnetic fields. In this context, a new experimental protocol is described, in which electron spins in a ferromagnetic material are redirected at the unprecedented level in cooperation with the enhanced THz magnetic field. Subsequently, the author demonstrates that the THz magnetic field is significantly amplified as a nearfield around the SRR structured metamaterial, which is implemented by measuring spin precession in a solid. At the end, the author presents the key experiment in which the amplified THz magnetic nearfield is applied to the weak ferromagnet ErFeO3 along with the femtosecond near-infrared pulse, demonstrating the successful control of symmetry breaking of the spin system due to coherent control of the optically-induced spin reorientation phase transition pathways. The comprehensive introductory review in this book allows readers to overview state-of-the-art terahertz spectroscopic techniques. In addition, the skillful description of the experiments is highly informative for readers in ultrafast magnonics, ultrafast optics, terahertz technology and plasmonic science.

Replication, the independent confirmation of experimental results and conclusions, is regarded as the "gold standard" in science. This book examines the question of successful or failed replications and demonstrates that that question is not always easy to answer. It presents clear examples of successful replications, the discoveries of the Higgs boson and of gravity waves. Failed replications include early experiments on the Fifth Force, a proposed modification of Newton's Law of universal gravitation, and the measurements of "G," the constant in that law. Other case studies illustrate some of the difficulties and complexities in deciding whether a replication is successful or failed. It also discusses how that question has been answered. These studies include the "discovery" of the pentaquark in the early 2000s and the continuing search for neutrinoless double beta decay. It argues that although successful replication is the goal of scientific experimentation, it is not always easily achieved.

The detection and measurement of the dynamic regulation and interactions of cells and proteins within the living cell are critical to the understanding of cellular biology and pathophysiology. The multidisciplinary field of molecular imaging of living subjects continues to expand with dramatic advances in chemistry, molecular biology, therapeutics, engineering, medical physics and biomedical applications. Molecular Imaging: Principles and Practice, Volumes 1 and 2, Second Edition provides the first point of entry for physicians, scientists, and practitioners. This authoritative reference book provides a comprehensible overview along with in-depth presentation of molecular imaging concepts, technologies and applications making it the foremost source for both established and new investigators, collaborators, students and anyone interested in this exciting and important field.