This handy reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally-intact cells from mammalian endocrine tissues, and their maintenance as primary cultures. The cell types selected for coverage illustrate the diversity of endocrine tissues from which cells have been isolated, and the range of procedures which have been devised to ensure the optimal survival and behaviour of each cell type under study. Particular emphasis has been placed on the provision of detailed protocols describing, step by step, the manipulations necessary to establish differentiated and responsive cultures. The chapters have been prepared by authors having direct practical experience of the cell type concerned, and the reader is therefore provided with first-hand accounts on the background to each procedure, the avoidance of potential problems and pitfalls, and the fundamental questions in endocrinology which may be addressed using each cell culture model.

Science teachers come in many varieties, but they share a common goal: to nurture learners. Over the past decade, we have learned a great deal about how to do this effectively. Of all this new (and some not so new) knowledge, what strikes me as most important is that learning occurs best within a context. Still, as obvious as that may seem, it is relatively rare in our high school science classrooms. The problem, of course, is that it is not easy to create a learning experience with hands-on relevance to the science under discussion. Science teachers, in addition to not having the the time, for the most part do not have the expertise or readily available resources. The solution lies in finding ways to bring scientists into the teaching/learning equation. Scientists teamed with teachers and their students represent a very real and rich opportunity to involve students in real science as practiced. Imagine a research book that gives examples of honest, science-research experiences for science-oriented students. What's more, imagine a book that includes examples where students are collaborating with scientists from all over the world on research projects, in person or via the Internet. Internet Linksfor Science Education does just that. It explores the role of the Internet and technol ogy in working student-scientist partnerships.

In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

Matrix isolation is a technique used for studying short-lived atoms and molecules at very low temperatures. This book offers detailed practical advice on how to carry out matrix-isolation experiments, and is a unique introduction to the subject. It is an essential practical text that covers a range of topics, from how to build a matrix-isolation laboratory from scratch, to detailed instructions for carrying out experiments.

This 1993 book gives a comprehensive account of both experimental and theoretical aspects of electron microprobe analysis, and is an extensively updated version of the seminal first edition, published in 1975. The design and operation of the instrument, including the electron column and both wavelength- and energy-dispersive X-ray spectrometers, are covered in the first part of the book. Experimental procedures for qualitative and quantitative analysis, using both types of spectrometer, are then discussed. Matrix ('ZAF') corrections, as required for quantitative analysis, are treated in some detail from both theoretical and practical viewpoints. Special considerations applying to the analysis of 'light' elements (atomic number below 10) are covered in a separate chapter. The emphasis throughout is on a sound understanding of principles and the treatment is applicable equally to the electron microprobe in its 'classical' form and to scanning electron microscopes fitted with X-ray spectrometers.

Unrivalled in its coverage and unique in its hands-on approach, this guide to the design and construction of scientific apparatus is essential reading for every scientist and student of engineering, and physical, chemical, and biological sciences. Covering the physical principles governing the operation of the mechanical, optical and electronic parts of an instrument, new sections on detectors, low-temperature measurements, high-pressure apparatus, and updated engineering specifications, as well as 400 figures and tables, have been added to this edition. Data on the properties of materials and components used by manufacturers are included. Mechanical, optical, and electronic construction techniques carried out in the lab, as well as those let out to specialized shops, are also described. Step-by-step instruction supported by many detailed figures, is given for laboratory skills such as soldering electrical components, glassblowing, brazing, and polishing.

Forrest M. Mims is a revered contributor to Make: magazine, where his popular columns about science-related topics and projects for Makers are evergreen treasures. Collected together here for the first time, these columns range from such simple projects as building an LED tracker for hand-launched night rockets to such challenging builds as transforming strings of data into unique musical compositions. A variety of photography and imaging projects are featured, including an ultra-sensitive twilight photometer that measures the elevation of layers of dust, smoke, and smog from around 3,000 feet to the top of the stratosphere at 31 miles! Most of the projects can be done with a collection of simple electronic components, such as LEDs, transistors, resistors, and batteries. To inspire and motivate readers, the book also includes profiles of such famous Makers as President Thomas Jefferson and Microsoft co-founder Paul Allen.

The aim of the Handbooks in Practical Animal Cell Biology is to provide practical workbooks for those involved in primary cell culture. Each volume addresses a different cell lineage, and contains an introductory section followed by individual chapters on the culture of specific differentiated cell types. The authors of each chapter are leading researchers in their fields and use their first-hand experience to present reliable techniques in a clear and thorough manner. Epithelial Cell Culture contains chapters on epithelial cells derived from 1) airway, 2) intestine, 3) pancreas, 4) kidney and bladder, 5) genital ducts, 6) mammary glands, 7) skin glands and appendages, and 8) keratinocytes.

The aim of the Handbooks in Practical Animal Cell Biology is to provide practical workbooks for those involved in primary cell culture. Each volume addresses a different cell lineage, and contains an introductory section followed by individual chapters on the culture of specific differentiated cell types. The authors of each chapter are leading researchers in their fields and use their first-hand experience to present reliable techniques in a clear and thorough manner. Epithelial Cell Culture contains chapters on epithelial cells derived from 1) airway, 2) intestine, 3) pancreas, 4) kidney and bladder, 5) genital ducts, 6) mammary glands, 7) skin glands and appendages, and 8) keratinocytes.

The aim of the Handbooks in Practical Animal Cell Biology is to provide practical workbooks for those involved in primary cell culture. Each volume addresses a different cell lineage, and contains an introductory section followed by individual chapters on the culture of specific differentiated cell types. The authors of each chapter are leading researchers in their fields and use their first-hand experience to present reliable techniques in a clear and thorough manner. Endothelial Cell Culture contains chapters on endothelial cells derived from 1) lung, 2) bone marrow, 3) brain, 4) mammary glands, 5) skin, 6) adipose tissue, 7) female reproductive system, and 8) synovium.

"The Ultimate Book of Saturday Science" is Neil Downie's biggest and most astounding compendium yet of science experiments you can do in your own kitchen or backyard using common household items. It may be the only book that encourages hands-on science learning through the use of high-velocity, air-driven carrots.

Downie, the undisputed maestro of Saturday science, here reveals important principles in physics, engineering, and chemistry through such marvels as the Helevator--a contraption that's half helicopter, half elevator--and the Rocket Railroad, which pumps propellant up from its own track. The Riddle of the Sands demonstrates why some granular materials form steep cones when poured while others collapse in an avalanche. The Sunbeam Exploder creates a combustible delivery system out of sunlight, while the Red Hot Memory experiment shows you how to store data as heat. Want to learn to tell time using a knife and some butter? There's a whole section devoted to exotic clocks and oscillators that teaches you how.

"The Ultimate Book of Saturday Science" features more than seventy fun and astonishing experiments that range in difficulty from simple to more challenging. All of them are original, and all are guaranteed to work. Downie provides instructions for each one and explains the underlying science, and also presents experimental variations that readers will want to try.

The use of primates in research is an ongoing controversy. We have all benefited from the medical discoveries, yet we have also learned more in recent years about the real intelligence of apes and monkeys. Activists have also uncovered cases of animal cruelty by researchers.

The Monkey Wars assesses the often caustic debate over the use of primates in scientific research, and examines the personalities and issues behind the headlines. The author focuses on researchers forced to conduct their work behind barbed wire and alarm systems, animal rights activists ranging from the moderate AWI Institute to the highly radical ALF, and some of the remarkable chimpanzees involved. The research community and its activist critics are invariably portrayed as rival camps locked in a long, bitter, and seemingly intractable political battle. In reality there are people on both sides willing to accept and work within the complex middle. Deborah Blum gives these people a voice

The investigation and manipulation of matter on the atomic scale have been revolutionized by scanning tunneling microscopy and related scanning probe techniques. This book is the first to provide a clear and comprehensive introduction to this subject. Beginning with the theoretical background of scanning tunneling microscopy, the design and instrumentation of practical STM and associated systems are described in detail, including topographic imaging, local tunneling barrier height measurements, tunneling spectroscopy, and local potentiometry. A treatment of the experimental techniques used in scanning force microscopy and other scanning probe techniques rounds out this section. The second part discusses representative applications of these techniques in fields such as condensed matter physics, chemistry, materials science, biology, and nanotechnology, so this book will be extremely valuable to upper-division students and researchers in these areas.

The functional anatomy of human somatosensory cortex is of both scientific and clinical interest. Scientifically, it provides insights in information processing in the human brain. Clinically, it helps to avoid neurological deficits by sparing essential brain regions during neurosurgical procedures adjacent to central fissure. In the present study the functional organization of the human somatosensory cortex was investigated with electrophysiological techniques using a combined approach of cortical stimulations and somatosensory evoked responses on electrocorticography, scalp-EEG, and magnetoencephalography. The spatiotemporal structure of the evoked response was studiedwith biophysical modeling techniqueswhich allowed identification of the three-dimensional intracerebral location, time activity, and interaction of the neuronal sources activated following peripheral somatosensory stimulation. Furthermore, the somatotopic organization ofhand and lip somatosensory cortex was investigated. The relative value of invasive (cortical stimulations and electrocorticography) and non-invasive (scalp-EEGand magnetoencephalography) was assessed. The combined use ofscalp-EEG and magnetoencephalography was useful to increase non-invasive localization accuracy. I want to thank several people who significantly contributed in completion of the present work. Univ. -Prof Dr. Luder Deecke, Chairman of the Neurological University Clinic, Vienna, Austria, supported me throughout my career at the Neurological University Clinic in Vienna since 1985. Dr. William W Sutherling, Associate Professor at the University of California, Los Angeles, who was my advisor during my stay from 1987-1989 at the Department ofNeurology, University of California, Los Angeles where most of the present work was done.

Microbes create medicines, filter waste water, and clean pollution. They give cheese funky flavors, wines complex aromas, and bread a nutty crumb. Life at the Edge of Sight is a stunning visual exploration of the inhabitants of an invisible world, from the pioneering findings of a seventeenth-century visionary to magnificent close-ups of the inner workings and cooperative communities of Earth's most prolific organisms. Using cutting-edge imaging technologies, Scott Chimileski and Roberto Kolter lead readers through breakthroughs and unresolved questions scientists hope microbes will answer soon. They explain how microbial studies have clarified the origins of life on Earth, guided thinking about possible life on other planets, unlocked evolutionary mechanisms, and helped explain the functioning of complex ecosystems. Microbes have been harnessed to increase crop yields and promote human health. But equally impressive, Life at the Edge of Sight opens a beautiful new frontier for readers to explore through words and images. We learn that there is more microbial biodiversity on a single frond of duckweed floating in a Delft canal than the diversity of plants and animals that biologists find in tropical rainforests. Colonies with millions of microbes can produce an array of pigments that put an artist's palette to shame. The microbial world is ancient and ever-changing, buried in fossils and driven by cellular reactions operating in quadrillionths of a second. All other organisms have evolved within this universe of microbes, yielding intricate beneficial symbioses. With two experts as guides, the invisible microbial world awaits in plain sight.

The compound optical microscope, in its various modern forms, is probably the most familiar of all laboratory instruments and the electron microscope, once an exotic rarity, has now become a standard tool in biological and materials research. Both instruments are often used effectively with little knowledge of the relevant theory, or even of how a particular type of microscope functions. Eventually however, proper use, interpretation of images and choices of specific applications demand an understanding of fundamental principles. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, accounts for ultimate limits on the size of observable details (resolving power and resolution) and finally provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. The book will appeal to graduate students and researchers in the biomedical sciences, and it will be helpful to students taking a course involving the principles of microscopy.

Flow cytometry is now well established in research laboratories and is gaining increasing use in clinical medicine and pathology. The technique enables multiple simultaneous light scatter and fluorescence measurements to be made at the individual cell level at very rapid rates and results in very large quantities of data being collected. Data, however, is just a series of numbers which have to be converted to information which, in turn, must be shown to have meaning. This is the most important single aspect of flow cytometry but it has received relatively little attention. One of the frequently voiced advantages of the technology is that it produces 'good statistics' because large numbers of cells have been analysed. However, it is not very often that confidence limits are placed on results, hence the reader has little or no feel for the inherent variability in the information produced. This book covers very basic number handling techniques, regression analysis, probability functions, statistical tests and methods of analysing dynamic processes. All those who use flow cytometry in their research will find this book an invaluable guide to interpreting the data produced by flow cytometers.

A unique introduction to the design, analysis, and presentation of scientific projects, this is an essential textbook for undergraduate majors in science and mathematics. The textbook gives an overview of the main methods used in scientific research, including hypothesis testing, the measurement of functional relationships, and observational research. It describes important features of experimental design, such as the control of errors, instrument calibration, data analysis, laboratory safety, and the treatment of human subjects. Important concepts in statistics are discussed, focusing on standard error, the meaning of p values, and use of elementary statistical tests. The textbook introduces some of the main ideas in mathematical modeling, including order-of-magnitude analysis, function fitting, Fourier transforms, recursion relations, and difference approximations to differential equations. It also provides guidelines on accessing scientific literature, and preparing scientific papers and presentations. An extensive instructor's manual containing sample lessons and student papers is available at www.cambridge.org/Marder.

A complete examination of the uses of the atomic force microscope in biology and medicine

This cutting-edge text, written by a team of leading experts, is the first detailed examination of the latest, most powerful scanning probe microscope, the atomic force microscope (AFM). Using the AFM, in combination with conventional tools and techniques, readers gain a profound understanding of the cell, subcellular organelles, and biomolecular structure and function.
The text begins with three chapters describing the molecular machinery and mechanism of cell secretion and membrane fusion in cells, using approaches that combine AFM, electron microscopy, X-ray diffraction, photon correlation spectroscopy, molecular biology, biochemistry, and electrophysiology. The discovery of a new cellular structure the "porosome" or fusion pore--the cells secretory machinery, the molecular mechanism of membrane fusion in cells, and the expulsion of intravesicular contents during cell secretion are outlined in the first three chapters. The book also covers:
* Identification of the "porosome" in the growth hormone secreting cell of the pituitary gland
* Probing the structural and physical properties of microbial cell surfaces
* Scanning probe microscopic characterization of the higher plant cell wall and its components
* Case studies of nano drug delivery systems using engineered dendrimers
* AFM techniques for studying living cells
* Investigating the intermolecular forces of leukocyte adhesion molecules
* Protein-protein interactions
* Micromechanical properties of lipid bilayers and vesicles
The text concludes with four chapters that examine new and emerging approaches in the use of force microscopy in biology and medicine.
This text is ideal for advanced undergraduate and graduate students and researchers in cell and molecular biology, genetics, genomics, physiology, neuroscience, biophysics, and biochemistry. Not only does it provide the theory, but also practical considerations such as the selection of the right tools and approach.

This book is designed to supply research workers in biochemistry and related fields with factual information about the compounds, reagents, and techniques they use most frequently in the laboratory. The material has been selected by practising biochemists and, in this third edition, a wide range of data is clearly displayed in an easily accessible form. Much has been deleted from earlier editions to be replaced by new material of current importance to biochemistry and molecular biology. Functional grouping of compounds has been emphasized to enable users of this book to see what compounds are available as well as find data on specific examples. Now available in paperback, this book provides a clear presentation of the type of information frequently needed in experimental work and is an invaluable laboratory companion for workers in any aspect of biochemistry. `Highly recommended as a standard reference source in biochemical research.' Journal of Histochemistry and Cytochemistry

The first book to chronicle how innovation in laboratory designs for botanical research energized the emergence of physiological plant ecology as a vibrant subdiscipline   Laboratory innovation since the mid-twentieth century has powered advances in the study of plant adaptation, evolution, and ecosystem function. The phytotron, an integrated complex of controlled-environment greenhouse and laboratory spaces, invented by Frits W. Went in the 1950s, set off a worldwide laboratory movement and transformed the plant sciences. Sharon Kingsland explores this revolution through a comparative study of work in the United States, France, Australia, Israel, the USSR, and Hungary.   These advances in botanical research energized physiological plant ecology. Case studies explore the development of phytotron spinoffs such as mobile laboratories, rhizotrons, and ecotrons. Scientific problems include the significance of plant emissions of volatile organic compounds, symbiosis between plants and soil fungi, and the discovery of new pathways for photosynthesis as an adaptation to hot, dry climates. The advancement of knowledge through synthesis is a running theme: linking disciplines, combining laboratory and field research, and moving across ecological scales from leaf to ecosystem. The book also charts the history of modern scientific responses to the emerging crisis of food insecurity in the era of global warming.

Raymond E. Barrett's Build-It-Yourself Science Laboratory is a classic book that took on an audacious task: to show young readers in the 1960s how to build a complete working science lab for chemistry, biology, and physics--and how to perform experiments with those tools. The experiments in this book are fearless and bold by today's standards--any number of the experiments might never be mentioned in a modern book for young readers! Yet, many from previous generations fondly remember how we as a society used to embrace scientific learning. This new version of Barrett's book has been updated for today's world with annotations and updates from Windell Oskay of Evil Mad Scientist Laboratories, including extensive notes about modern safety practices, suggestions on where to find the parts you need, and tips for building upon Barrett's ideas with modern technology. With this book, you'll be ready to take on your own scientific explorations at school, work, or home.

Spark scientific curiosity from a young age with this six-level course through an enquiry-based approach and active learning. Collins International Primary Science fully meets the requirements of the Cambridge Primary Science Curriculum Framework from 2020 and has been carefully developed for a range of international contexts. The course is organised into four main strands: Biology, Chemistry, Physics and Earth and Space and the skills detailed under the 'Thinking and Working Scientifically' strand are introduced and taught in the context of those areas. For each Workbook at Stages 1 to 6, we offer: A write-in Workbook linked to the Student's Book New language development activities help build science vocabulary Earth and Space content covers the new curriculum framework Thinking and Working Scientifically deepens and enhances the delivery of Science skills Actively learn through practical activities that don't require specialist equipment or labs Scaffolding allows students of varying abilities to work with common content and meet learning objectives Supports Cambridge Global Perspectives (TM) with activities that develop and practise key skills Provides learner support as part of a set of resources for the Cambridge Primary Science curriculum framework (0097) from 2020 This series is endorsed by Cambridge Assessment International Education to support the new curriculum framework 0097 from 2020.

Textbook of "in vivo" Imaging in Vertebrates.

Editors.

Vasilis Ntziachristos

Department of Radiology, Harvard University HMS/MGH, Charlestown, USA

Anne Leroy-Willig

U2R2M, CNRS and Universite Paris-Sud, Orsay, France

Bertrand Tavitian

Unite d'Imagerie de l'Expression des Genes, INSERM, Orsay, Franc

This book describes the new imaging techniques being developed to monitor physiological, cellular and subcellular function within living animals. This exciting field of imaging science brings together physics, chemistry, engineering, biology and medicine to yield powerful and versatile imaging approaches. By combining advanced non-invasive imaging technologies with new mechanisms for visualizing biochemical events and protein and gene function, non-invasive vertebrate imaging enables the in vivo study of biology and offers rapid routes from basic discovery to drug development and clinical application. Combined with the availability of an increasing number of animal models of human disease, and the ability to perform longitudinal studies of disease evolution and of the long-term effects of therapeutic procedures, this new technology offers the next generation of tools for biomedical research.

Well illustrated, largely in colour, the book reviews the most common and technologically advanced methods for vertebrate imaging, presented in a clear, comprehensive format. The basic principles are described, followed by several examples of the use of imaging in the study of living multicellular organisms, concentrating on small animal models of human diseases. The book illustrates:

- The types of information that can be obtained with modern in vivo imaging;

-The substitution of imaging methods for more destructive histological techniques;

- The advantages conferred by in vivo imaging in building a more accurate picture of the response of tissues to stimuli over time while significantly reducing the number of animals required for such studies.

Part 1 describes current techniques in in vivo imaging, providing specialists and laboratory scientists from all disciplines with clear and helpful information regarding the tools available for their specific research field. Part 2 looks in more detail at imaging organ development and function, covering the brain, heart, lung and others. Part 3 describes the use of imaging to monitor various new types of therapy, following the reaction in an individual organism over time, e.g. after gene or cell therapy.

Most chapters are written by teams of physicists and biologists, giving a balanced coherent description of each technique and its potential applications.

The book is aimed at a broad audience conducting research in areas such as biochemistry, physiology, developmental biology, oncology and pharmacology. While written primarily for those already engaged in imaging studies, it will also be of interest to scientists from other disciplines looking for an entry point into the field of in vivo imaging in small animals.