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Books > Professional & Technical > Industrial chemistry & manufacturing technologies > Industrial chemistry
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High-Performance Liquid Chromatography of Peptides and Proteins - Separation, Analysis, and Conformation (Paperback)
Loot Price: R2,132
Discovery Miles 21 320
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High-Performance Liquid Chromatography of Peptides and Proteins - Separation, Analysis, and Conformation (Paperback)
Expected to ship within 12 - 17 working days
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This book consists of a series of 82 precise, easy-to-read articles
by internationally renowned scientists and emphasizes the practical
approach to HPLC with minimal theory, although the underlying
principles for peptide and protein separations are clearly
expressed. All of the major modes of microbore, ultrafast and
analytical HPLC are discussed, including size-exclusion,
ion-exchange, reversed-phase, hydrophobic interaction, and affinity
and immunoaffinity chromatography. A section on preparative HPLC,
including displacement techniques, is also presented.
Problem-solving approaches to the separation of various classes of
biologically active peptides and proteins are thoroughly explored,
while the importance of peptide standards for monitoring column
performance and for optimizing separation conditions is emphasized.
Several articles focus on the choice of the correct detection
method (electrochemical, UV, fluorescence), as well as the need for
a proper knowledge of approaches to column and instrument
maintenance and trouble-shooting. A section on predictive
approaches deals with both computer simulation of peptide
separations and peptide structure. The book also includes
complementary techniques to HPLC, as well as other useful
applications of HPLC. It enables both novice and experienced
chromatographers to realize the full potential of this extremely
powerful technique, in the process making an important contribution
to scientific literature.
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