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Ultrastructure Techniques for Microorganisms (Paperback, Softcover reprint of the original 1st ed. 1986)
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Ultrastructure Techniques for Microorganisms (Paperback, Softcover reprint of the original 1st ed. 1986)
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The modem microbiologist is often a real specialist who has
difficulty under standing and applying many of the techniques
beyond those in his or her own immediate field. On the other hand,
most benefits to modem microbiology are obtained when a broad
spectrum of scientific approaches can be focused on a problem. In
early studies, electron microscopy was pivotal in understanding
bacterial and viral morphology, and we still feel that we will
understand a disease better if we have seen an electron micrograph
of the causative agent. Today, because there is an increased
awareness of the need to understand the rela tionships between
microbial structure and function, the electron microscope is still
one of the most important tools microbiologists can use for
detailed analysis of microorganisms. Often, however, the
aforementioned modem microbiologist still thinks of ultrastructure
as involving negative staining or ultrathin sectioning in order to
get a look at the shape of a "bug. " Many of the newer
ultrastructure techniques, such as gold-labeled antibody
localization, freeze-fracture, X-ray microanalysis, enzyme
localization, and even scanning electron microscopy, are poorly
under stood by, and therefore forbidding to, the average
microbiologist. Even many cell biologists admit to having
difficulty staying in touch with current develop ments in the
fast-moving field of electron microscopy techniques."
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