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Chicken Egg Yolk Antibodies, Production and Application - IgY-Technology (Paperback, 2001)
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Chicken Egg Yolk Antibodies, Production and Application - IgY-Technology (Paperback, 2001)
Series: Springer Lab Manuals
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Polyclonal antibodies, widely used in research and diagnostics, are
conventionally isolated from the blood of immunised mammals. The
fact that antibodies can also be detected in the yolk of eggs laid
by immunised hens, led to the development of the yolk antibody
technology as an alternative method less stressful to animals. Hens
can be kept under nearly natural conditions and antibodies be
isolated from the eggs.
Further, the IgY-technology has the advantage that avian antibodies
can be produced against several antigens which do not induce a
immune response in mammals. Protocols on how to keep and immunise
hens and on the extraction, isolation and use of antibodies from
yolk are described in detail in this manual.
For their work on yolk antibodies the authors received the FISEA
(International Foundation for the Substitution of Animal
Experimentation, Luxembourg) prize in 1997.
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