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Books > Science & Mathematics > Physics > Applied physics & special topics > Biophysics
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Photophysics of Ionic Biochromophores (Hardcover, 2013 ed.)
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Photophysics of Ionic Biochromophores (Hardcover, 2013 ed.)
Series: Physical Chemistry in Action
Expected to ship within 12 - 17 working days
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This book provides a concise overview of the photophysics and
spectroscopy of bio chromophore ions. The book "Photophysics of
Ionic Biochromophores" summarizes important recent advances in the
spectroscopy of isolated biomolecular ions in vacuo, which has
within the last decade become a highly active research field.
Advanced instrumental apparatus and the steady increase in more and
more powerful computers have made this development possible, both
for experimentalists and theoreticians. Applied techniques
described here include absorption and fluorescence spectroscopy,
which are excellent indicators of environmental effects and can
thus shed light on the intrinsic electronic structures of ions
without perturbations from e.g. water molecules, counter ions,
nearby charges, and polar amino acid residues. When compared with
spectra of the chromophores in their natural environment, such
spectra allow to identify possible perturbations. At the same time
gas-phase spectra provide important benchmarks for quantum
chemistry calculations of electronically excited states. This
volume focuses on biological systems from protein biochromophores,
e.g. the protonated Schiff-base retinal responsible for vision, and
individual aromatic amino acids to peptides and whole proteins,
studied using visible, ultraviolet and vacuum ultraviolet light.
Work on DNA nucleotides and strands that are amenable to mass
spectrometric studies because of the negatively charged
sugarphosphate backbone are also presented. DNA strands represent
an example of the interplay between multiple chromophores, which is
even harder to model correctly than just single chromophores due to
spatially extended excited states and weak coupling terms. The
experimental techniques used to measure spectra and commonly used
theoretical methods are described with a discussion on limitations
and advantages. The volume includes an updated status of the field
and interesting future directions such as cold ion spectroscopy.
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