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Blood Debts
Terry J Benton-Walker
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R403
R328
Discovery Miles 3 280
Save R75 (19%)
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Cancer treatments have been revolutionised over the past 40 years,
and women are increasingly expected to take an active part in their
breast cancer care, and are often faced with complex choices to
make. But, a busy and stressful hospital clinic typically gives
little time for detailed explanations, and, with the bewildering
range of treatments on offer today, how do you know which one is
best for you? This book gives a historical overview of the
therapies, and gives the basic information needed to make informed
choices about cancer care.
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Blood Debts
Terry J Benton-Walker
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R280
R224
Discovery Miles 2 240
Save R56 (20%)
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PERFECT FOR FANS OF CASSANDRA CLAIRE AND TRACY DEONN! 'An
extravaganza from start to finish.' Chloe Gong, New York Times
bestselling author of These Violent Delights 'Riveting.
Unflinching. Powerful.' Ayana Gray, New York Times-bestselling
author of Beasts of Prey 'Sings with hope and barely disguised
rage. Fantastic from start to finish'' TJ Klune, New York Times
bestselling author of The House in the Cerulean Sea In New Orleans,
gods meddle, and magic will betray you, but this time justice will
reign. Thirty years ago, a young woman was murdered, a family was
lynched, and New Orleans saw the greatest magical massacre in its
history. In the days that followed, a throne was stolen from a
queen. On the anniversary of these brutal events, Clement and
Cristina Trudeau - the sixteen-year-old twin heirs to the powerful,
magical, dethroned family - are mourning their father and caring
for their sick mother. Until, by chance, they discover their mother
isn't sick - she's cursed. Cursed by someone on the very magic
council their family used to rule. Someone who will come for them
next. Cristina, once a talented and dedicated practitioner of
Generational magic, has given up magic for good. An ancient spell
is what killed their father and she was the one who cast it. For
Clement, magic is his lifeline. A distraction from his anger and
pain. Even better than the random guys he hooks up with. Cristina
and Clement used to be each other's most trusted confidant and
friend, now they barely speak. But if they have any hope of
discovering who is coming after their family, they'll have to find
a way to trust each other and their family's magic, all while
solving the decades - old murder that sparked the still - rising
tensions between the city's magical and non-magical communities.
And if they don't succeed, New Orleans may see another massacre. Or
worse. Praise for Blood Debts 'Riveting and relevant' Kirkus
Reviews, Starred review 'Blood Debts is a force and Terry J.
Benton-Walker is truly an author to watch' Roseanne A. Brown, New
York Times bestselling author of A Song of Wraiths and Ruin
'Steeped in magic that's every bit as dark as it is moving' Alexis
Henderson, author of The Year of the Witching and House of Hunger
'The real magic is this book-don't let it slip through your
fingers' Mark Oshiro, author of Anger is a Gift 'Intricately woven
threads of magic, intergenerational trauma, and well-placed social
commentary' Nic Stone, New York Times bestselling author of Dear
Martin 'Sharp and searing' J. Elle, New York Times bestselling
author of Wings of Ebony
The definitive 30th-anniversary exploration of the beloved Jim
Henson classic, featuring rare artwork, interviews, and on-set
photos. Filled with a wealth of rare and unseen behind-the-scenes
imagery, experience the film's creation as seen through the eyes of
the artists, costume designers, and creature creators who gave the
beloved fantasy classic its distinctive look. Featuring in-depth
interviews with the talented crew and cast, and story notes from
Henson.
This Volume presents methods for analysing and quantifying
petroleum, hydrocarbons and lipids, based on their chemical and
physical properties as well as their biological effects. It
features protocols for extracting hydrocarbons from solid matrices,
water and air, and a dedicated chapter focusing on volatile organic
compounds. Several approaches for separating and detecting diverse
classes of hydrocarbons and lipids are described, including:
(tandem) gas chromatography (GC) coupled with mass spectrometry
(MS) or flame-ionisation detection, Fourier-transform
induction-coupled-resonance MS, and fluorescence-based techniques.
The book details high-performance liquid chromatography MS for
microbial lipids, as well as a combination of techniques for
naphthenic acids. Two chapters focus on quantifying bioavailable
hydrocarbon fractions by using cyclodextrin sorbents and bacterial
bioreporters, respectively, while a closing chapter explains how
compound-specific stable-isotope analysis can be used to measure
the fate of hydrocarbons in the environment. Hydrocarbon and Lipid
Microbiology Protocols There are tens of thousands of structurally
different hydrocarbons, hydrocarbon derivatives and lipids, and a
wide array of these molecules are required for cells to function.
The global hydrocarbon cycle, which is largely driven by
microorganisms, has a major impact on our environment and climate.
Microbes are responsible for cleaning up the environmental
pollution caused by the exploitation of hydrocarbon reservoirs and
will also be pivotal in reducing our reliance on fossil fuels by
providing biofuels, plastics and industrial chemicals. Gaining an
understanding of the relevant functions of the wide range of
microbes that produce, consume and modify hydrocarbons and related
compounds will be key to responding to these challenges. This
comprehensive collection of current and emerging protocols will
facilitate acquisition of this understanding and exploitation of
useful activities of such microbes.
This Volume describes methods for cultivating hydrocarbon-producing
and -consuming microbes, covering compounds in a range of states -
gaseous (e.g. methane), liquid (e.g. alkanes of intermediate
molecular weight) and solid (e.g. many PAHs and asphaltene). It
also examines the cultivation of aerobic and anaerobic hydrocarbon
degraders using a range of electron acceptors (e.g. oxygen,
nitrate, sulphate, metals, (per)chlorate), and a separate chapter
is devoted to explaining the cultivation of methanogens. Special
attention is given to: high-throughput cultivation, growing
microbes as biofilms, and cultivating fastidious microbes, as well
as the preservation of microbial pure cultures and consortia.
Accordingly, this Volume will be of value to anyone embarking on
the selective enrichment and cultivation of novel microorganisms.
Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of
thousands of structurally different hydrocarbons, hydrocarbon
derivatives and lipids, and a wide array of these molecules are
required for cells to function. The global hydrocarbon cycle, which
is largely driven by microorganisms, has a major impact on our
environment and climate. Microbes are responsible for cleaning up
the environmental pollution caused by the exploitation of
hydrocarbon reservoirs and will also be pivotal in reducing our
reliance on fossil fuels by providing biofuels, plastics and
industrial chemicals. Gaining an understanding of the relevant
functions of the wide range of microbes that produce, consume and
modify hydrocarbons and related compounds will be key to responding
to these challenges. This comprehensive collection of current and
emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
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Avila Beach (Hardcover)
Terry J. San Filippo, Jack San Filippo, Pete Kelley
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R702
Discovery Miles 7 020
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Ships in 12 - 17 working days
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This Volume presents methods for quantifying microbial populations
and characterising microbial communities by extracting and
analysing biomarkers such as RNA, DNA and lipids. The chapters
cover a wide range of topics, including: cell separation from
oil-rich environments, enumeration of hydrocarbon degraders and
sulphate reducers using most-probable-number techniques, and
quantification by means of real-time PCR. A variety of molecular
methods are described for microbial community profiling, such as
phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One
chapter examines high-throughput sequencing, and provides important
information on the associated procedures required for thorough data
analysis. A further chapter is devoted to the characterisation of
protistan communities, while the closing chapter describes
multiplex fluorescent antibody microarrays for detecting microbial
biomarkers. Hydrocarbon and Lipid Microbiology Protocols There are
tens of thousands of structurally different hydrocarbons,
hydrocarbon derivatives and lipids, and a wide array of these
molecules are required for cells to function. The global
hydrocarbon cycle, which is largely driven by microorganisms, has a
major impact on our environment and climate. Microbes are
responsible for cleaning up the environmental pollution caused by
the exploitation of hydrocarbon reservoirs and will also be pivotal
in reducing our reliance on fossil fuels by providing biofuels,
plastics and industrial chemicals. Gaining an understanding of the
relevant functions of the wide range of microbes that produce,
consume and modify hydrocarbons and related compounds will be key
to responding to these challenges. This comprehensive collection of
current and emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
This Volume presents protocols for investigating the genetic,
metabolic and ecological potential and functional analysis of
microbial communities. Methods are described for the creation and
bioinformatic assessment of metagenomic and metatranscriptomic
libraries, and for metaproteomic analyses, which provide important
insights into the metabolic potential and interactions of community
members. These in turn lead to specific hypotheses concerning the
functional contributions of individual populations in the
community, which may be investigated by the stable isotope probing
approaches described in this Volume, making it possible to identify
those community members primarily responsible for particular
functions. Methods for the direct extraction of proteins from
environmental samples for sequencing and activity tests are
presented, providing a broad overview of prevailing metabolic
activities and of the types of microbe involved in them. Protocols
for the analysis of nutrient flow through microbial communities and
for the modelling of dynamic physiological interactions in
communities are also provided. Lastly, the book presents a protocol
for the quantitative assessment of permissiveness for the transfer
of conjugative plasmids, important agents of physiological change
and evolution in microbial communities. Hydrocarbon and Lipid
Microbiology ProtocolsThere are tens of thousands of structurally
different hydrocarbons, hydrocarbon derivatives and lipids, and a
wide array of these molecules are required for cells to function.
The global hydrocarbon cycle, which is largely driven by
microorganisms, has a major impact on our environment and climate.
Microbes are responsible for cleaning up the environmental
pollution caused by the exploitation of hydrocarbon reservoirs and
will also be pivotal in reducing our reliance on fossil fuels by
providing biofuels, plastics and industrial chemicals. Gaining an
understanding of the relevant functions of the wide range of
microbes that produce, consume and modify hydrocarbons and related
compounds will be key to responding to these challenges. This
comprehensive collection of current and emerging protocols will
facilitate acquisition of this understanding and exploitation of
useful activities of such microbes.
This Volume features protocols for investigating the hydrocarbon-
and lipid-specific activities of microbes. They include methods for
studying chemotaxis, the colonisation of hydrocarbon surfaces,
hydrocarbon uptake, respiration, nitrogen fixation, sulphate
reduction, membrane stabilisation through cis-trans isomerisation
of membrane fatty acids, and the production of biosurfactants and
biopolymers in response to the presence of hydrocarbons. A protocol
for studying the ability of microbes to control the concentration
of hydrocarbons in their aqueous environment is also described, and
phenotyping methods to reveal microbes' more general metabolic
activities are presented. Several protocols for investigating acid
production in connection with oil souring and biocorrosion by
microbes in oil well, oil transportation and storage settings are
presented. Lastly, protocols for measuring methanogenesis, as an
example of microbial hydrocarbon production, are described.<
Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of
thousands of structurally different hydrocarbons, hydrocarbon
derivatives and lipids, and a wide array of these molecules are
required for cells to function. The global hydrocarbon cycle, which
is largely driven by microorganisms, has a major impact on our
environment and climate. Microbes are responsible for cleaning up
the environmental pollution caused by the exploitation of
hydrocarbon reservoirs and will also be pivotal in reducing our
reliance on fossil fuels by providing biofuels, plastics and
industrial chemicals. Gaining an understanding of the relevant
functions of the wide range of microbes that produce, consume and
modify hydrocarbons and related compounds will be key to responding
to these challenges. This comprehensive collection of current and
emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
This Volume provides protocols for the biochemical analysis of
hydrocarbon- and lipid-relevant products, cell components and
activities of microbes that interact with hydrophobic compounds.
They include methods for the extraction, purification and
characterisation of surface tension-reducing bioemulsifiers and
biosurfactants that increase the surface area and hence
bioavailability of hydrophobic substrates. Protocols for the
isolation and biochemical analysis of lipids and
polyhydroxyalkanoates, food storage products made during nutrient
abundance that represent important biotechnological products, are
presented. The extraction of membrane lipid rafts, sub-organelles
that fulfil important functional roles for the cell membrane, and
the isolation and characterisation of membrane phospholipid
biomarkers, are also described. The purification and
characterisation of integral membrane hydrocarbon-oxidising enzymes
are addressed. Lastly, two generic methods for the genetic analysis
of catabolic pathways and analysis of ligand binding are presented.
Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of
thousands of structurally different hydrocarbons, hydrocarbon
derivatives and lipids, and a wide array of these molecules are
required for cells to function. The global hydrocarbon cycle, which
is largely driven by microorganisms, has a major impact on our
environment and climate. Microbes are responsible for cleaning up
the environmental pollution caused by the exploitation of
hydrocarbon reservoirs and will also be pivotal in reducing our
reliance on fossil fuels by providing biofuels, plastics and
industrial chemicals. Gaining an understanding of the relevant
functions of the wide range of microbes that produce, consume and
modify hydrocarbons and related compounds will be key to responding
to these challenges. This comprehensive collection of current and
emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
This Volume presents a comprehensive series of generic protocols
for the genetic and genomic analysis of prokaryotic isolates.
Genetic methods for functional analyses employ the latest cloning
vectors, gene fusion methods and transposon mutagenesis systems, as
well as systems for introducing protease-cleavage sequences into
permissive sites in proteins under investigation. Genomic methods
described include protocols for transcriptomics, shotgun
proteomics, interactomics, metabolic profiling, and lipidomics.
Bioinformatic tools for genome annotation, transcriptome display
and the integration of transcriptomic data into genome-scale
metabolic reconstructions are described. Protocols for 13C-based
metabolic flux determinations and analysis of the hierarchical and
metabolic regulation of fluxes through pathways are included. The
Volume thus enables investigators to functionally analyse an
isolate over the entire cellular range spanning the gene, the
genome, the transcript repertoire, the proteome, the interactome,
the metabolic network with its nodes and their regulatory
hierarchies, and the metabolic fluxes and their physiological
controls. Hydrocarbon and Lipid Microbiology Protocols There are
tens of thousands of structurally different hydrocarbons,
hydrocarbon derivatives and lipids, and a wide array of these
molecules are required for cells to function. The global
hydrocarbon cycle, which is largely driven by microorganisms, has a
major impact on our environment and climate. Microbes are
responsible for cleaning up the environmental pollution caused by
the exploitation of hydrocarbon reservoirs and will also be pivotal
in reducing our reliance on fossil fuels by providing biofuels,
plastics and industrial chemicals. Gaining an understanding of the
relevant functions of the wide range of microbes that produce,
consume and modify hydrocarbons and related compounds will be key
to responding to these challenges. This comprehensive collection of
current and emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
This Volume presents key microscopy and imaging methods for
revealing the structure and ultrastructure of environmental and
experimental samples, of microbial communities and cultures, and of
individual cells. Method adaptations that specifically address
problems concerning the hydrophobic components of samples are
highlighted and discussed. The methods described range from
electron microscopy and light and fluorescence microscopy, to
confocal laser-scanning microscopy, and include experimental
set-ups for the analysis of interfacial processes like microbial
growth and activities at hydrocarbon:water interfaces, biofilms and
microbe:mineral interfaces. Three forms of fluorescence in situ
hybridization - CARD-FISH, MAR-FISH and Two-pass TSA-FISH - are
described for the ecophysiological analysis of functionally active
microbes in samples. The methods presented will enable readers to
obtain an ultrastructural picture of, and identify the key
functional microbes in, samples under investigation. This in turn
will constitute a key framework for the interpretation of
information from other experimental approaches, such as
physicochemical analyses and genomic investigations. Hydrocarbon
and Lipid Microbiology ProtocolsThere are tens of thousands of
structurally different hydrocarbons, hydrocarbon derivatives and
lipids, and a wide array of these molecules are required for cells
to function. The global hydrocarbon cycle, which is largely driven
by microorganisms, has a major impact on our environment and
climate. Microbes are responsible for cleaning up the environmental
pollution caused by the exploitation of hydrocarbon reservoirs and
will also be pivotal in reducing our reliance on fossil fuels by
providing biofuels, plastics and industrial chemicals. Gaining an
understanding of the relevant functions of the wide range of
microbes that produce, consume and modify hydrocarbons and related
compounds will be key to responding to these challenges. This
comprehensive collection of current and emerging protocols will
facilitate acquisition of this understanding and exploitation of
useful activities of such microbes.
This Volume presents generic protocols for wet experimental and
computer-based systems and synthetic biology approaches relevant to
the field of hydrocarbon and lipid microbiology. It complements a
second Volume that describes protocols for systems and synthetic
biology applications. The wet experimental tools presented in this
Volume include protocols for the standardisation of transcriptional
measurements, application of uracil excision-based DNA editing for,
inter alia, multi-gene assembly, the use of fluxomics to optimise
"reducing power availability", and the incorporation of
non-canonical amino acids into proteins for optimisation of
activities. Phenome-ing microbes, using a combination of RNA-seq
and bioinformatic algorithms, is presented, as is an illustration,
using methylotrophs as an example, of how the different key omics
approaches constitute a pipeline for functional analysis,
acquisition of a systems overview, and metabolic optimisation.
Complementary computational tools that are presented include
protocols for probing the genome architecture of regulatory
networks, genome-scale metabolic reconstruction, and bioinformatic
approaches to guide metabolic engineering. The Volume also includes
an overview of how synthetic biology approaches can be used to
improve biocontainment. Hydrocarbon and Lipid Microbiology
ProtocolsThere are tens of thousands of structurally different
hydrocarbons, hydrocarbon derivatives and lipids, and a wide array
of these molecules are required for cells to function. The global
hydrocarbon cycle, which is largely driven by microorganisms, has a
major impact on our environment and climate. Microbes are
responsible for cleaning up the environmental pollution caused by
the exploitation of hydrocarbon reservoirs and will also be pivotal
in reducing our reliance on fossil fuels by providing biofuels,
plastics and industrial chemicals. Gaining an understanding of the
relevant functions of the wide range of microbes that produce,
consume and modify hydrocarbons and related compounds will be key
to responding to these challenges. This comprehensive collection of
current and emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
This Volume addresses the pros and cons of oligonucleotide probes,
primers and primer combinations, and importantly considers how to
design the best tools for the microbial taxa and/or processes being
investigated. Individual chapters focus on the design of primers
targeting genes that code for enzymes associated with the following
functions: degradation of aromatic, aliphatic and chlorinated
hydrocarbons under aerobic and anaerobic conditions,
methanogenesis, methane oxidation, and the nitrogen cycle.
Hydrocarbon and Lipid Microbiology Protocols There are tens of
thousands of structurally different hydrocarbons, hydrocarbon
derivatives and lipids, and a wide array of these molecules are
required for cells to function. The global hydrocarbon cycle, which
is largely driven by microorganisms, has a major impact on our
environment and climate. Microbes are responsible for cleaning up
the environmental pollution caused by the exploitation of
hydrocarbon reservoirs and will also be pivotal in reducing our
reliance on fossil fuels by providing biofuels, plastics and
industrial chemicals. Gaining an understanding of the relevant
functions of the wide range of microbes that produce, consume and
modify hydrocarbons and related compounds will be key to responding
to these challenges. This comprehensive collection of current and
emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
This Volume presents relevant single-cell and single-molecule
approaches in the study of microbes producing and utilizing
hydrocarbons and lipids. While generically applicable for all
microorganisms, the approaches described are, wherever possible,
adapted to the field of study of hydrocarbon and lipid
microbiology. The methods include basic procedures for isolating
single cells by means of microfluidics and flow cytometry, and
their cultivation in arrays as pure clones; for isolating,
amplifying and sequencing single-cell genomes and transcriptomes;
and for analysing single-cell metabolomes by means of Raman
spectroscopy. Single-molecule approaches include the use of
protein:fluorescent dye fusions for protein localization and
methods for the production of cell division protostructures and
lipid monolayers. Methods for the functional analysis of single
cells include detection of metabolically active
(protein-synthesizing) cells in environmental samples by
bioorthogonal non-canonical amino acid tagging, Raman spectroscopy
combined with stable isotope labelling and fluorescent in situ
hybridisation, and visualization of single cells participating in
gene transfer activity. Lastly, protocols are presented for
single-cell biotechnological applications, including biofuel
production. Hydrocarbon and Lipid Microbiology ProtocolsThere are
tens of thousands of structurally different hydrocarbons,
hydrocarbon derivatives and lipids, and a wide array of these
molecules are required for cells to function. The global
hydrocarbon cycle, which is largely driven by microorganisms, has a
major impact on our environment and climate. Microbes are
responsible for cleaning up the environmental pollution caused by
the exploitation of hydrocarbon reservoirs and will also be pivotal
in reducing our reliance on fossil fuels by providing biofuels,
plastics and industrial chemicals. Gaining an understanding of the
relevant functions of the wide range of microbes that produce,
consume and modify hydrocarbons and related compounds will be key
to responding to these challenges. This comprehensive collection of
current and emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
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Blood Justice
Terry J Benton-Walker
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R592
R465
Discovery Miles 4 650
Save R127 (21%)
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Ships in 10 - 15 working days
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This book is a compilation of the research which was presented
during the NATO-Advanced Research Workshop (ARW) entitled "Advances
in Bacterial Paracrystalline Surface Layers" held in London,
Ontario, Canada during September 27 to 30, 1992. The organizing
committee consisted of the two Workshop directors, S. F. Kaval and
T. J. Beveridge, and H. Konig, U. B. Sleytr and T. J. Trust; their
summary statements about the significance and success of the
NATO-ARWare in Chapter 37 of this book. This was the third
international workshop on bacterial S-layers and it demonstrated
unequivocally how rapidly research is progressing. The Workshop was
made possible by financial support from the North Atlantic Treaty
Organization (NATO), the Medical Research Council of Canada (MRC),
the Natural Seiences and Engineering Research Council of Canada
(NSERC), and the Canadian Bacterial Diseases Network (CBDN) which
is a Canadian National Centre of Excellence (NCE). We are very
grateful for the support from all of these agencies since their
financial aid made it possible to bring to London, Canada a truly
international group of S-layer experts. We encouraged the
attendance and participation of graduate fellows and research
associates, and their presentations students, postdoctoral was an
intense three constitute the "Poster" section of this book. The
NATO-ARW day workshop held at a delightful secluded location
(Spencer Hall) so that the delegates had both formal and informal
occasions to interact and evolve new ideas."
This Volume presents protocols for systems and synthetic biology
applications in the field of hydrocarbon and lipid microbiology. It
complements another Volume that describes generic protocols for wet
experimental and computer-based systems and synthetic biology
studies. The protocols in this Volume demonstrate how to employ
systems and synthetic biology approaches in the design of microbes
for the production of esters, isoprenoids, hydrophobic polymers,
rhamnolipid biosurfactant, and peptide antimicrobial and
thioether-stabilised molecules. Also presented is a protocol for
the engineering of transcription factor-based biosensors for
intracellular products, and another for the creation of a synthetic
hydroxylase with novel activity for the selective
oxyfunctionalisation of linear alkanes. Hydrocarbon and Lipid
Microbiology ProtocolsThere are tens of thousands of structurally
different hydrocarbons, hydrocarbon derivatives and lipids, and a
wide array of these molecules are required for cells to function.
The global hydrocarbon cycle, which is largely driven by
microorganisms, has a major impact on our environment and climate.
Microbes are responsible for cleaning up the environmental
pollution caused by the exploitation of hydrocarbon reservoirs and
will also be pivotal in reducing our reliance on fossil fuels by
providing biofuels, plastics and industrial chemicals. Gaining an
understanding of the relevant functions of the wide range of
microbes that produce, consume and modify hydrocarbons and related
compounds will be key to responding to these challenges. This
comprehensive collection of current and emerging protocols will
facilitate acquisition of this understanding and exploitation of
useful activities of such microbes.
Arising from a research project on depression in the eighteenth
century, this book discusses the experience of depressive states
both in terms of existing modes of thought and expression, and of
attempts to describe and live with suffering. It also asks what
present-day society can learn about depression from the
eighteenth-century experience.
This Volume describes methods for investigating microbes in their
natural environment and how to obtain representative samples and
preserve them for subsequent analyses. Chapters are arranged
according to the environments under investigation, which include:
oil reservoirs, fracking fluids, aquifers, coal beds, oil sands and
their tailing ponds, lakes, rivers, leaves, polar seas and ice, the
sea-surface microlayer, mud flats, microbialites, and deep-sea
fauna. A variety of downstream analytical procedures are described,
including: nucleic-acid extraction and preparation for
high-throughput sequencing, fluorescence in-situ hybridisation, and
cultivation of aerobic and anaerobic hydrocarbon-degrading
microbes. Though most chapters focus on hydrocarbon-rich
environments, many of the approaches used are generic, and as such
will be of value to researchers embarking on studies of microbes
and their processes in the field. Hydrocarbon and Lipid
Microbiology Protocols There are tens of thousands of structurally
different hydrocarbons, hydrocarbon derivatives and lipids, and a
wide array of these molecules are required for cells to function.
The global hydrocarbon cycle, which is largely driven by
microorganisms, has a major impact on our environment and climate.
Microbes are responsible for cleaning up the environmental
pollution caused by the exploitation of hydrocarbon reservoirs and
will also be pivotal in reducing our reliance on fossil fuels by
providing biofuels, plastics and industrial chemicals. Gaining an
understanding of the relevant functions of the wide range of
microbes that produce, consume and modify hydrocarbons and related
compounds will be key to responding to these challenges. This
comprehensive collection of current and emerging protocols will
facilitate acquisition of this understanding and exploitation of
useful activities of such microbes.
|
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