The growing scale of plant-based chemicals for industrial use has generated considerable interest in developing methods to meet their desired production levels. Among various available strategies for their production, the development of Agrobacterium rhizogenes mediated hairy root cultures (HRCs) is generally considered the most feasible approach. Additionally, several proof-of-principle experiments have demonstrated the practical feasibility of HRCs in the plant-based remediation of environment pollutants, biotransformation of important compounds, and production of therapeutic proteins. Given that hairy root biotechnology has now been recognized as a promising and highly dynamic research area, this book offers a timely update on recent advances, and approaches hairy roots as a multifaceted biological tool for various applications. Further, it seeks to investigate the loopholes in existing methodologies, identify remaining challenges and find potential solutions by presenting well thought-out scientific discussions from various eminent research groups working on hairy root biotechnology. This book provides detailed conceptual and practical information on HRC-based research, along with relevant case studies. The content is divided into three broad sections, namely (i) Hairy Roots and Secondary Metabolism, (ii) Progressive Applications, and (iii) Novel Approaches and Future Prospects. By informing the research and teaching community about the major strides made in HRC-based interventions in plant biology and their applications, the book is sure to spark further research in this fascinating field.

This six volume book set examines a range of topics and applications related to biotechnology. Volumes include fermentation and algal biotechnologies; agricultural biotechnology; medical biotechnology, biopharmaceutics; biosafety, bioethics, biotechnology policy; microbiomes; bioenergy and environmental biotechnology for sustainable development. The topics address significant aspects of the dairy and fermented foods; crop genetics, breeding and genomics; microalgae and novel products; molecular diagnostics and DNA forensics; biosafety, bioethics and legal issues in biotechnology; biotechnology policy advocacy, enlightenment and engagement with stakeholders; medical and pharmaceutical biotechnology; omics and bioinformatics; waste recycling, biofuels and environmental remediation; animal genetics, breeding and genomics; genetic resources conservation and utilization; medicinal and underutilized plants; medical insect biotechnology, genomics and molecular genetics of pests; microbiomes and microbial biotechnology; biotechnology education and curriculum development; and water and waste water research. Volumes include: I: Fermentation and Algal Biotechnologies for the Food, Beverage and other Bioproduct Industries II: Agricultural Biotechnology, Biodiversity and Bio-resources Conservation and Utilization III: Medical Biotechnology, Biopharmaceutics, Forensic Science and Bioinformatics IV: Biosafety and Bioethics in Biotechnology: Policy, Advocacy, and Capacity Building V: Microbiomes and Emerging Applications VI: Bioenergy and Environmental Biotechnology for Sustainable Development The book is a timely knowledge product that documents key issues on advances in biotechnology for use by a variety of readers including postgraduate students, professionals in the field, policy makers, science advocacy groups.

This book provides an up-to-date overview of redox signaling in plant cells and its key role in responses to different stresses. The chapters, which are original works or reviews, focus on redox signaling states; cellular tolerance under different biotic and abiotic stresses; cellular redox homeostasis as a central modulator; redox homeostasis and reactive oxygen species (ROS); redox balance in chloroplasts and mitochondria; oxidative stress and its role in peroxisome homeostasis; glutathione-related enzyme systems and metabolism under metal stress; and abiotic stress-induced redox changes and programmed cell death. The book is an invaluable source of information for plant scientists and students interested in redox state chemistry and cellular tolerance in plants.

The population of the world continues to increase at an alarming rate. The trouble linked with overpopulation ranges from food and water scarcity to inadequacy of space for organisms. Overpopulation is also linked with several other demographic hazards, for instance, population blooming will not only result in exhaustion of natural repositories, but it will also induce intense pressure on the world economy. Today nanotechnology is often discussed as a key discipline of research but it has positive and negative aspects. Also, due to industrialization and ever-increasing population, nano-pollution has been an emerging topic among scientists for investigation and debate. Nanotechnology measures any substance on a macromolecular scale, molecular scale, and even atomic scale. More importantly, nanotechnology deals with the manipulation and control of any matter at the dimension of a single nanometer. Nanotechnology and nanoparticles (NPs) play important roles in sustainable development and environmental challenges as well. NPs possess both harmful and beneficial effects on the environment and its harboring components, such as microbes, plants, and humans. There are many beneficial impacts exerted by nanoparticles, however, including their role in the management of waste water and soil treatment, cosmetics, food packaging, agriculture, biomedicines, pharmaceuticals, renewable energies, and environmental remedies. Conversely, NPs also show some toxic effects on microbes, plants, as well as human beings. It has been reported that use of nanotechnological products leads to the more accumulation of NPs in soil and aquatic ecosystems, which may be detrimental for living organisms. Further, toxic effects of NPs on microbes, invertebrates, and aquatic organisms including algae, has been measured. Scientists have also reported on the negative impact of NPs on plants by discussing the delivery of NPs in plants. Additionally, scientists have also showed that NPs interact with plant cells, which results in alterations in growth, biological function, gene expression, and development. Thus, there has been much investigated and reported on NPs and plant interactions in the last decade. This book discusses the most recent work on NPs and plant interaction, which should be useful for scientists working in nanotechnology across a wide variety of disciplines.

Plant Circadian Networks: Methods and Protocols provides a collection of protocols to investigate clock-controlled parameters including transcript and small RNA levels, promoter activity using luciferase reporters, protein levels and posttranslational modification, protein-protein interaction, in vivo DNA-protein interaction and RNA-protein interaction, cellular redox state, Ca2+ levels, and innate immune responses. Furthermore, the use of bioinformatics resources is described to evaluate high throughput data sets and to integrate the data into an overarching picture of circadian networks in the cell. Additional chapters focus on seasonal processes like flowering time control, and techniques on trees, moss and algae. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Circadian Networks: Methods and Protocols is designed not only for the chronobiology community dealing with circadian biology but also for the plant community in general.

This book aims to help plant breeders by reviewing past achievements, currently successful practices, and emerging methods and techniques. Theoretical considerations are also presented to strike the right balance between being as simple as possible but as complex as necessary. The United Nations predicts that the global human population will continue rising to 9.0 billion by 2050. World food production will need to increase between 70-100 per cent in just 40 years. First generation bio-fuels are also using crops and cropland to produce energy rather than food. In addition, land area used for agriculture may remain static or even decrease as a result of degradation and climate change, despite more land being theoretically available, unless crops can be bred which tolerate associated abiotic stresses. Lastly, it is unlikely that steps can be taken to mitigate all of the climate change predicted to occur by 2050, and beyond, and hence adaptation of farming systems and crop production will be required to reduce predicted negative effects on yields that will occur without crop adaptation. Substantial progress will therefore be required in bridging the yield gap between what is currently achieved per unit of land and what should be possible in future, with the best farming methods and best storage and transportation of food, given the availability of suitably adapted cultivars, including adaptation to climate change. My book is divided into four parts: Part I is an historical introduction; Part II deals with the origin of genetic variation by mutation and recombination of DNA; Part III explains how the mating system of a crop species determines the genetic structure of its landraces; Part IV considers the three complementary options for future progress: use of sexual reproduction in further conventional breeding, base broadening and introgression; mutation breeding; and genetically modified crops.

This book compiles the latest applications of the cutting-edge gene editing tool CRISPR/Cas in the area of crop improvement. It begins with an introduction to the technique and its application in crop plants. Next, it gives an updated overview of available delivery methods, design tools and resources in CRISPR/Cas. The book subsequently reviews the applications of CRISPR/Cas in connection with e.g. insect stress, disease stress, abiotic stress, nutritional and yield improvement in crop plants, etc. It also discusses the various regulatory, ethical and social aspects of the technique that must be kept in mind when designing experiments. In closing, the book summarizes the status quo and outlines future prospects for the tool in crop improvement and food security. Given its scope, the book will especially benefit students and researchers in food science, biotechnology, agriculture and the plant sciences.

A book focused solely on Andean Cloud Forests (ACF) has never been published. ACF are high biodiversity ecosystems in the Neotropics with a large proportion of endemic species, and are important for the hydrology of entire regions. They provide water for large parts of the Amazon basin, for example. Here I take advantage of my many years working in ACF in Ecuador, to edit this book that contains the following sections: (1) ACF over space and time, (2) Hydrology, (3) Light and the Carbon cycle, (4) Soil, litter, fungi and nutrient cycling, (5) Plants, (6) Animals, and (7) Human impacts and management. Under this premise, international experts contributed chapters that consist of reviews of what is known about their topic, of what research they have done, and of what needs to be done in the future. This work is suitable for graduate students, professors, scientists, and researcher-oriented managers.

This book is a compilation of information on insect/mite/vertebrate pests and fungal/bacterial/viral/mycoplasma/nematode diseases of tropical root and tuber crops such as cassava, sweet potato, yams, taro, Amorphophallus, yam bean and tannia. The book highlights the distribution, symptoms and damage, biology, survival and spread of each pest and describes management methods. It also sheds light on different eco-friendly pest management strategies including physical, cultural, chemical, biological, host resistance and integrated methods. The book is written in a lucid style using easy-to-understand language and offers adoptable recommendations involving eco-friendly control measures. It serves as a useful reference source for policy makers, research and extension workers, practicing farmers and students. The material can also be used for teaching post graduate courses in state agricultural universities.

The Science of Grapevines: Anatomy and Physiology is an introduction to the physical structure of the grapevine, its various organs, their functions and their interactions with the environment. Beginning with a brief overview of the botanical classification (including an introduction to the concepts of species, cultivars, clones, and rootstocks), plant morphology and anatomy, and growth cycles of grapevines, The Science of Grapevines covers the basic concepts in growth and development, water relations, photosynthesis and respiration, mineral uptake and utilization, and carbon partitioning. These concepts are put to use to understand plant-environment interactions including canopy dynamics, yield formation, and fruit composition, and concludes with an introduction to stress physiology, including water stress (drought and flooding), nutrient deficiency and excess, extreme temperatures (heat and cold), and the impact and response to of other organisms. Based on the author's years of teaching grapevine anatomy as well as his research experience with grapevines and practical experience growing grapes, this book provides an important guide to understanding the entire plant.

Seaweeds (macroalgae) represent the most striking living components in the Antarctic's near-shore ecosystems, especially across the West Antarctic Peninsula and adjacent islands. Due to their abundance, their central roles as primary producers and foundation organisms, and as sources of diverse metabolically active products, seaweed assemblages are fundamental to biogeochemical cycles in Antarctic coastal systems. In recent years, the imminence of climate change and the direct impacts of human beings, which are affecting vast regions of the Antarctic, have highlighted the importance of seaweed processes in connection with biodiversity, adaptation and interactions in the benthic network. Various research groups have been actively involved in the investigation of these topics. Many of these research efforts have a long tradition, while some "newcomers" have also recently contributed important new approaches to the study of these organisms, benefiting polar science as a whole. This book provides an overview of recent advances and insights gleaned over the past several years. Focusing on a timely topic and extremely valuable resource, it assesses the challenges and outlines future directions in the study of Antarctic seaweeds.

Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes. This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species. Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species. For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. Volume 1 details updated techniques available for 18 plant species drawn from cereal crops, legume plants, vegetable plants, and three model plant species: Brachypodium distachyon, Medicago truncatula, and Setaria viridis. It also updates a chapter for vector construction, a step critical to a successful plant transformation process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers for use in both fundamental and applied biology.

This book provides the latest information about hairy root culture and its several applications, with special emphasis on potential of hairy roots for the production of bioactive compounds. Due to high growth rate as well as biochemical and genetic stability, it is possible to study the metabolic pathways related to production of bioactive compounds using hairy root culture. Chapters discuss the feasibility of hairy roots for plant derived natural compounds. Advantages and difficulties of hairy roots for up-scaling studies in bioreactors are included as well as successful examples of hairy root culture of plant species producing bioactive compounds used in food, flavors and pharmaceutical industry. This book is a valuable resource for researchers and students working on the area of plant natural products, phytochemistry, plant tissue culture, medicines, and drug discovery.

This book provides all aspects of the physiology, stress responses and tolerance to abiotic stresses of the Brassicaceae plants. Different plant families have been providing food, fodder, fuel, medicine and other basic needs for the human and animal since the ancient time. Among the plant families, Brassicaceae has special importance for their agri-horticultural importance and multifarious uses apart from the basic needs. Interest understanding the response of Brassicaceae plants toward abiotic stresses is growing considering the economic importance and the special adaptive mechanisms. The knowledge needs to be translated into improved elite lines that can contribute to achieve food security. The physiological and molecular mechanisms acting on Brassicaceae introduced in this book are useful to students and researchers working on biology, physiology, environmental interactions and biotechnology of Brassicaceae plants.

An introduction to the principles of membrane transport: How molecules and ions move across the cell membrane by simple diffusion and by making use of specialized membrane components (channels, carriers, and pumps). The text emphasizes the quantitative aspects of such movement and its interpretation in terms of transport kinetics. Molecular studies of channels, carriers, and pumps are described in detail as well as structural principles and the fundamental similarities between the various transporters and their evolutionary interrelationships. The regulation of transporters and their role in health and disease are also considered.

Dendroecologists apply the principles and methods of tree-ring science to address ecological questions and resolve problems related to global environmental change. In this fast-growing field, tree rings are used to investigate forest development and succession, disturbance regimes, ecotone and treeline dynamics and forest decline. This book of global scope highlights state-of-the-science dendroecological contributions to paradigm-shifts in our understanding of ecophysiology, stand dynamics, disturbance interactions, forest decline and ecosystem resilience to global environmental change and is fundamental to better managing our forested ecosystems for the full range of ecosystem goods and services that they provide.

Roy Ellen's The Nuaulu World of Plants is the culmination of anthropological fieldwork on the eastern Indonesian island of Seram, and of comparative enquiries into the bases of human classificatory activity through the study of ethnobiological knowledge over a fifty year period. This rich account of the ways plants feature in the worldview and lifeways of the Nuaulu, recognizes that plant knowledge is embedded in plural local and historical contexts: in swiddens, garden crops, managed fallow, village spaces and pathways; in the trees, and the ecological, conceptual and experiential relationships to forest; in plants' roles as healing agents, raw materials, fuels and in ritual; and in historical flux, with the introduction of exotic plants and the impact of colonial and post-colonial ways of seeing the plant world. Ellen's contemporary examination of Nuaulu classificatory practices, in the light of comparable observations made by the seventeenth-century Dutch naturalist Rumphius, allows us to better see how scientific taxonomy emerges from folk knowledge. The comprehensive study of local plant classification based on robust datasets and long-term fieldwork presented here is a rare achievement, and comprises an outstanding resource for regional ethnology. But this book offers a further dimension, evaluating the theoretical consensus on the relationship between so-called 'natural' classifications and utilitarian schemes, and thereby highlights, and addresses, some of the problems of Berlin and Atran's highly influential framework for studying folk knowledge systems. It emphasizes the difficulties of simple claims for universality versus relativity, cultural models versus individual contextual schemata, and of two-dimensional taxonomies. Ellen persuasively argues that classification is a dynamic and living process of cultural cognition that links knowledge to practice, and is not easily reducible to graphical representations or abstract generalizations. Moreover, he draws attention to recent radical approaches to ontology and epistemology, specifically those focusing upon 'convergence metaphysics', arguing these present new challenges for the field. 'This book will undoubtedly become a landmark study in the field of ethnobotany. It represents anthropology at its best ... Roy Ellen has an outstanding reputation and is recognised globally as a leading ethnoscientist, and this rich volume further confirms his status.' Paul Sillitoe FBA, Professor of Anthropology, Durham University This will be a must read for students interested in conducting ethnobiological fieldwork and, more broadly, comparative analysis of cognition... Nuggets of gold come in every chapter. Thomas Thorton, Associate Professor & Senior Associate Research Fellow, University of Oxford