Genetic approaches to understanding plant growth and development have always benefitted from screens that are simple, quantitative and rapid. Visual screens and morphometric analysis have yielded a plethora of interesting mutants and traits that have provided insight into complex regulatory pathways, and yet many genes within any given plant genome remain undefined. The premise underlying High Throughput Phenotyping in Plants: Methods and Protocols is that the higher the resolution of the phenotype analysis the more likely that new genes and complex interactions will be revealed. The methods described in this volume can be generally classified as quantitative profiling of cellular components, ranging from ions to small molecule metabolites and nuclear DNA, or image capture that ranges in resolution from chlorophyll fluorescence from leaves and time-lapse images of seedling shoots and roots to individual plants within a population at a field site. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, High Throughput Phenotyping in Plants: Methods and Protocols serves as an invaluable guide to plant researchers and all scientists who wish to better understand plant growth and development.

This is the first collection of essays in which European and American philosophers explicitly think out their respective contributions and identities as environmental thinkers in the analytic and continental traditions. The American/European, as well as Analytic/Continental collaboration here bears fruit helpful for further theorizing and research. The essays group around three well-defined areas of questioning all focusing on the amelioration/management of environmentally, historically and traditionally diminished landscapes. The first part deals with differences between New World and the Old World perspectives on nature and landscape restoration in general, the second focuses on the meaning of ecological restoration of cultural landscapes, and the third on the meaning of the wolf and of wildness. It does so in a way that the strengths of each philosophical school-continental and analytic-comes to the fore in order to supplement the other's approach. This text is open to educated readers across all disciplines, particularly those interested in restoration/adaptation ecology, the cultural construction of place and landscape, the ongoing conversation about wilderness, the challenges posed to global environmental change. The text may also be a gold mine for doctoral students looking for dissertation projects in environmental philosophy that are inclusive of continental and analytic traditions. This text is rich in innovative approaches to the questions they raise that are reasonably well thought out. The fact that the essays in each section really do resonate with one another directly is also intellectually exciting and very helpful in working out the full dimensions of each question raised in the volume.

With today's ever growing economic and ecological problems, wood as a raw material takes on increasing significance as the most important renewable source of energy and as industrial feedstock for numerous products. Its chemical and anatomical structure and the excellent properties that result allow wood to be processed into the most diverse products; from logs to furniture and veneers, and from wood chippings to wooden composites and paper. The aim of this book is to review advances in research on the cellular aspects of cambial growth and wood formation in trees over recent decades. The book is divided into two major parts. The first part covers the basic process of wood biosynthesis, focusing on five major steps that are involved in this process: cell division, cell expansion, secondary cell wall formation, programmed cell death and heartwood formation. The second part of the book deals with the regulation of wood formation by endogenous and exogenous factors. On the endogenous level the emphasis is placed on two aspects: control of wood formation by phytohormones and by molecular mechanisms. Apart from endogenous factors, various exogenous effects (such as climate factors) are involved in wood formation. Due to modern microscopic as well as molecular techniques, the understanding of wood formation has progressed significantly over the last decade. Emphasizing the cellular aspects, this book first gives an overview of the basic process of wood formation, before it focuses on factors involved in the regulation of this process.

This six volume book set examines a range of topics and applications related to biotechnology. Volumes include fermentation and algal biotechnologies; agricultural biotechnology; medical biotechnology, biopharmaceutics; biosafety, bioethics, biotechnology policy; microbiomes; bioenergy and environmental biotechnology for sustainable development. The topics address significant aspects of the dairy and fermented foods; crop genetics, breeding and genomics; microalgae and novel products; molecular diagnostics and DNA forensics; biosafety, bioethics and legal issues in biotechnology; biotechnology policy advocacy, enlightenment and engagement with stakeholders; medical and pharmaceutical biotechnology; omics and bioinformatics; waste recycling, biofuels and environmental remediation; animal genetics, breeding and genomics; genetic resources conservation and utilization; medicinal and underutilized plants; medical insect biotechnology, genomics and molecular genetics of pests; microbiomes and microbial biotechnology; biotechnology education and curriculum development; and water and waste water research. Volumes include: I: Fermentation and Algal Biotechnologies for the Food, Beverage and other Bioproduct Industries II: Agricultural Biotechnology, Biodiversity and Bio-resources Conservation and Utilization III: Medical Biotechnology, Biopharmaceutics, Forensic Science and Bioinformatics IV: Biosafety and Bioethics in Biotechnology: Policy, Advocacy, and Capacity Building V: Microbiomes and Emerging Applications VI: Bioenergy and Environmental Biotechnology for Sustainable Development The book is a timely knowledge product that documents key issues on advances in biotechnology for use by a variety of readers including postgraduate students, professionals in the field, policy makers, science advocacy groups.

Lignins are nature s aromatic polymers and are the second most abundant organic constituent of the biosphere next to cellulose. Lignification mainly occurs in the walls of terrestrial vascular plants, mainly in the secondarily thickened cells of supportive or conductive tissues, which thus acquire novel properties.

This new volume of "Advances in Botanical Research" gives a special emphasis to the bioengineering of these enigmatic polymers. It is divided in nine chapters containing up-to-date reviews by expert groups in their field.
Gives a special emphasis to the bioengineering of these enigmatic polymers, ligninsDivided in nine chaptersContains up-to-date reviews by expert groups in their field"

This book reviews the current state of information on reactive oxygen and nitrogen species and their role in cell communication during plant growth, development and adaptation to stress conditions. It addresses current research advances made in the area of reactive oxygen and nitrogen species (ROS and RNS) signaling. These free radical molecules are important in plant-microbe interactions, responses to abiotic stress, stomatal regulation and a range of developmental processes. Due to their short half-life, high diffusion capability and ability to react with different components in the cell, ROS and RNS participate in various processes connected with signaling and communication in plants. The book's respective chapters address the latest advances made in the niche area of ROS and RNS in plants. It offers a valuable guide for researchers and students alike, providing insights into cutting-edge free radical research. The information on specialized topics presented is also highly relevant for applied fields such as food security, agricultural practices and medicinal use of plants.

Cultural landscapes are a product ofthe interactions between humans and natural settings. They are landscapes and seascapes that are shaped by human history and land use. Socioeconomic processes especially, but also environmental changes and natural disturbances, are some of the forces that make up landscape dynamics. To understand and manage such complex landscapes, interdisciplinary and transdisciplinary approaches are necessary, emphasizing the integration of natural and social sciences and considering multiple landscape functions. The spatial patterns of Asian landscapes are strongly related to human activities and their impacts. Anthropogenic patterns and processes have created numerous traditional cultural landscapes throughout the region, and understanding them requires indigenous knowledge. Cultural landscape ecology from a uniquely Asian perspective is explored in this book, as are the management of landscapes and land-use policies. Human-dominated landscapes with long traditions, such as those described herein, provideuseful information for all ecologists, not only in Asia, to better understand the human environmental relationship and landscape sustainability. "

A great deal of effort is being invested in understanding the molecular mechanisms through which plants interact with pathogenic microbes. In Plant Immunity: Methods and Protocols, expert researchers in the field describe emerging technologies that can be applied to the most significant outstanding questions faced by scientists studying immunity in plants. The technologies in this detailed volume include methods for examining protein localization, protein complex purification, protein-protein interactions, transient and inducible gene expression, chromatin immunoprecipitation, microaspiration, laser microdissection, purification of fungal haustoria, and genetic manipulation of bacterial and oomycete pathogens. These techniques are applicable to a wide range of topics, including molecular functionality of NB-LRR proteins and other immune signaling components, and functional characterization of effector proteins and other pathogen components that sabotage host immunity. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Phytoplasmas are a group of bacteria that are capable of multiplying and causing severe diseases in a wide range of plant species and are transported between plants by insect vectors in which the bacteria can also multiply. Phytoplasmas can cause serious and devastating problems for crop plants, especially in developing countries. In Phytoplasmas: Methods and Protocols experts in the field cover topics pertinent to scientists intending to develop a research program on phytoplasmas. The volume opens with an introductory chapter on the importance of phytoplasma diseases, followed by methods for detection and diagnosis as well as techniques for separating and classifying the phytoplasmas into their different taxonomic groups and subgroups. The final chapters cover methods for separating phytoplasma genomic and plasmid DNA from plant DNA for whole genome sequencing. Written in the successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Phytoplasmas: Methods and Protocols serves as an ideal guide to plant pathologists and molecular biologists aiming to set up a diagnostics facility for identifying the presence of these pathogens in plants or their insect vectors.

This volume presents the most recent studies on mRNA polyadenylation in plants. Chapters are divided into three sections covering recent development of the use of bioinformatics tools in the field. numerous molecular, biochemical, and methods used to characterize polyadenylation sites on a genome-wide scale. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Some are specific for plant research, but most can be adopted for research in other organisms. Authoritative and practical, Polyadenylation in Plants: Methods and Protocols provides scientists with a wide range of methods to study mRNA 3'-end formation in plants.

This book provides in-depth information on all key aspects of geminivirus biology, e.g. the genetics and evolution, global diversity and spread of these plant pathogens, as well as the molecular mechanisms underlying their virulence. Geminiviridae is one of the largest viral families, comprising numerous plant-infecting viruses that cause diseases in crops and weeds. These diseases have been reported from nearly all continents, in particular Asia, Europe, Africa and America. The book summarizes the current state of knowledge on the interactions between plant host and virus. In addition, it discusses advances regarding the trans-replication of satellite molecules and its effect on geminiviral pathogenesis, as well as pest management strategies to combat these diseases in the field. Given its scope, the book is a must-read reference guide for all researchers and advanced students working in virology, agriculture and plant biotechnology..

This is volume presents protocols relevant for plant receptor kinases(PRK) both in development and defence. Chapters guide readers through comprehensive experimental approach for molecular investigations of plant receptor kinases, from the simplest methods for expression and purification of receptor domains to the most advanced methods aiming at understanding the dynamics of receptor complex formation and specificity of signaling pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Receptor Kinases: Methods and Protocols aims to ensure successful results in the further study of this vital field.

This volume explores plastid evolution, structure, and function in algae, plants and protists. The methods described in this book help scientists visualize, fractionate, purify, and study primary and secondary plastids in plant and algal materials. The chapters in this book also look at various techniques to analyze plastids through means of combining biology strategies from genetics, genomics, proteomics, and lipidomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Plastids: Methods and Protocols is a valuable resource for students, engineers, and researchers who are interested in this evolving organelle and overall field.

How can cultivated plant biodiversity contribute to the transformation and the "ecologization" of agriculture in Southern countries? Based on extensive field work in the Southern countries, a great deal of scientific progress is presented in all areas affecting agriculture (agronomy, plant breeding and crop protection, cultivation systems, etc.) in order to intensify the ecological processes in cultivated plots and at the scale of rural landscapes.

The current volume, "Medicinal and Aromatic Plants of the Middle-East" brings together chapters on selected, unique medicinal plants of this region, known to man since biblical times. Written by leading researchers and scientists, this volume covers both domesticated crops and wild plants with great potential for cultivation. Some of these plants are well-known medicinally, such as opium poppy and khat, while others such as apharsemon and citron have both ritual and medicinal uses. All have specific and valuable uses in modern society. As such, it is an important contribution to the growing field of medicinal and aromatic plants. This volume is intended to bring the latest research to the attention of the broad range of botanists, ethnopharmacists, biochemists, plant and animal physiologists and others who will benefit from the information gathered therein. Plants know no political boundaries, and bringing specific folklore to general medical awareness can only be for the benefit of all.

This book provides an integrated approach to crop growth and development and the technical aspects of greenhouse cultivation and climate management. It combines an analysis of the relationship between crop production and ambient climate with an explanation of the processes that determine the climate in a protected environment. With the ability to modify the environment comes the need for growers to strike a balance between the costs and benefits of technology. This book outlines the methods and gives several examples of how to make 'optimal' choices about technology. Sustainable management of shoot and root environment is discussed, as well as the pros and cons of vertical farming. The processes addressed in this book, like crop growth, energy balance and mass exchange, apply to any kind of greenhouse. Therefore, in spite of the word 'technology', this is not a book about high-tech greenhouses only. 'Greenhouse horticulture' is an easy-to-read textbook for all those interested in protected cultivation, from university students and teachers to professional advisers in the field and managers of horticultural companies.

Ascorbic acid (AsA), vitamin C, is one of the most abundant water-soluble antioxidant in plants and animals. In plants AsA serves as a major redox buffer and regulates various physiological processes controlling growth, development, and stress tolerance. Recent studies on AsA homeostasis have broadened our understanding of these physiological events. At the mechanistic level, AsA has been shown to participate in numerous metabolic and cell signaling processes, and the dynamic relationship between AsA and reactive oxygen species (ROS) has been well documented. Being a major component of the ascorbate-glutathione (AsA-GSH) cycle, AsA helps to modulate oxidative stress in plants by controlling ROS detoxification alone and in co-operation with glutathione. In contrast to the single pathway responsible for AsA biosynthesis in animals, plants utilize multiple pathways to synthesize AsA, perhaps reflecting the importance of this molecule to plant health. Any fluctuations, increases or decreases, in cellular AsA levels can have profound effects on plant growth and development, as AsA is associated with the regulation of the cell cycle, redox signaling, enzyme function and defense gene expression. Although there has been significant progress made investigating the multiple roles AsA plays in stress tolerance, many aspects of AsA-mediated physiological responses require additional research if AsA metabolism is to be manipulated to enhance stress-tolerance. This book summarizes the roles of AsA that are directly or indirectly involved in the metabolic processes and physiological functions of plants. Key topics include AsA biosynthesis and metabolism, compartmentation and transport, AsA-mediated ROS detoxification, as well as AsA signaling functions in plant growth, development and responses to environmental stresses. The main objective of this volume is therefore to supply comprehensive and up-to-date information for students, scholars and scientists interested in or currently engaged in AsA research.

Estimation of the metabolite complement of plant material involves a wide range of techniques and technologies and that breadth continues to increase. Metabolomics research typically involves multiple sites for material preparation and analysis and most investigations are "high throughput", meaning that chemical analysis of sample sets are inevitably carried out over an extended period of time. In, Plant Metabolomics: Methods and Protocols expert researchers in the field detail many of the stages which are now commonly used to study plant metabolomics workflow. Stages of this workflow, up to and including the statistical analysis, accurate and detailed collection of meta-data are also essential for good process management, to satisfy reporting requirements and to ensure wider interpretability and reuse results.Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Through and intuitive Plant Metabolomics: Methods and Protocols, seeks to aid scientists in the further study of the methods for all the stages of the plant metabolomics workflow.

This three-book set is devoted to the prominent bays of the Western North America. The first volume describes San Francisco Bay - a shallow estuary surrounded by a large population center. The forces that built it began with plate tectonics and involved the collision of the Pacific and North American plates and the subduction of the Juan de Fuka plate. Gold mining during the California gold rush sent masses of slit into the Bay. The second volume is devoted to San Diego Bay, which is also a shallow estuary surrounded by a large human population center that influenced the Bay. The third volume describes Puget Sound - a different sort of bay - a complex fjord-estuary system, but also surrounded by several large population centers. The watershed is enormous, covering nearly 43,000 square kilometers with thousands of rivers and streams. Geological forces, volcanos, Ice Ages, and changes in sea levels make the Sound a biologically dynamic and fascinating environment, as well as a productive ecosystem. Key Features Summarizes a complex geological, geographical, and ecological history Reviews how the San Diego Bay has changed and will likely change in the future Examines the different roles of various drivers of Bay ecosystem function Includes the role of humans-both first people and modern populations-on the Bay Explores San Diego Bay as an example of general bay ecological and environmental issues