This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.

Plant conservation is increasingly recognised as an outstanding global priority, yet despite considerable efforts over the last few decades, the number of threatened species continues to rise. The practice of plant conservation has for too long been a rather hit-or-miss mixture of methods. While microorganisms have been recognised as a crucial and essential element in supporting the lifecycles of plant species, there has been limited recognition of the relationships between macro level conservation facilitating ecosystem functioning at the micro level.

This book addresses the role of microorganisms in conservation - both their support functions and deleterious roles in ecosystem processes and species survival. Importantly, a number of authors highlight how microbial diversity is, itself, now under threat from the many and pervasive influences of man. What is clear from this volume is that like many contemporary treatments of plant and animal conservation, the solution to mitigate the erosion of biodiversity is not simple. This book represents an attempt to bring to the fore the ecological underwriting provided by microorganisms.

As plants see it, humans are not the masters of the Earth but only one of its most unpleasant and irksome residents. They have been on the planet for only about 300,000 years ago (nothing compared to the three billon years of plant evolution), yet have changed the conditions of the planet so drastically as to make it a dangerous place for their own survival. It's time for the plants to offer advice. In this playful, philosophical manifesto, Stefano Mancuso, expert on plant intelligence, presents a new constitution on which to build our future as beings respectful of the Earth and its inhabitants. These eight articles - the fundamental pillars on which plant life is based - must henceforth regulate all living beings.

Early anthropological evidence for plant use as medicine is 60,000 years old as reported from the Neanderthal grave in Iraq. The importance of plants as medicine is further supported by archeological evidence from Asia and the Middle East. Today, around 1.4 billion people in South Asia alone have no access to modern health care, and rely instead on traditional medicine to alleviate various symptoms. On a global basis, approximately 50 to 80 thousand plant species are used either natively or as pharmaceutical derivatives for life-threatening conditions that include diabetes, hypertension and cancers. As the demand for plant-based medicine rises, there is an unmet need to investigate the quality, safety and efficacy of these herbals by the "scientific methods". Current research on drug discovery from medicinal plants involves a multifaceted approach combining botanical, phytochemical, analytical, and molecular techniques. For instance, high throughput robotic screens have been developed by industry; it is now possible to carry out 50,000 tests per day in the search for compounds which act on a key enzyme or a subset of receptors. This and other bioassays thus offer hope that one may eventually identify compounds for treating a variety of diseases or conditions. However, drug development from natural products is not without its problems. Frequent challenges encountered include the procurement of raw materials, the selection and implementation of appropriate high-throughput bioassays, and the scaling-up of preparative procedures. Research scientists should therefore arm themselves with the right tools and knowledge in order to harness the vast potentials of plant-based therapeutics. The main objective of Plant and Human Health is to serve as a comprehensive guide for this endeavor. Volume 1 highlights how humans from specific areas or cultures use indigenous plants. Despite technological developments, herbal drugs still occupy a preferential place in a majority of the population in the third world and have slowly taken roots as alternative medicine in the West. The integration of modern science with traditional uses of herbal drugs is important for our understanding of this ethnobotanical relationship. Volume 2 deals with the phytochemical and molecular characterization of herbal medicine. Specifically, It will focus on the secondary metabolic compounds which afford protection against diseases. Lastly, Volume 3 focuses on the physiological mechanisms by which the active ingredients of medicinal plants serve to improve human health. Together this three-volume collection intends to bridge the gap for herbalists, traditional and modern medical practitioners, and students and researchers in botany and horticulture.

It was around 1970, I had just completed a 5-year breeding project aiming at fxing fower colour in gerbera progenies: white, yellow, pink, and red; colour homogeneity was sound, but size and shape still required some improvement. The problem was defnitely resolved by Murashige and Skoog, USA who published a reliable protocol for gerbera micropro- gation. In short, my gerbera seed lines were immediately rendered obsolete by this e- cient cloning system, able to produce millions of plants of a matchless and previously unknown homogeneity, the uniformity of fower shape and colour being the basic requi- ments for the market. The success of micropropagation resulted in a tremendous growth in gerbera fower production worldwide, and this species conquered a leading place in the foriculture industry. This personal experience stresses the impact of micropropagation on the genetic improvement research strategies in ornamentals. Micropropagation has become "in- sive", especially in ornamental plant material issues. Today, hundreds of protocols exist; however, only a modest percentage of them are exploited economically. Thus, only micropropagation of plants with a high market price range, like orchids for instance, has proved cost-effective and achieved great success. Micropropagation is a labour-intensive system: hand-power is estimated to rep- sent 60-70% of total costs. This explains the outsourcing of the major labs in developing countries where labour is cheaper. Nevertheless, certain industrial protocols remain a proprietary technology of leading labs, mostly western, with the exception of Japan and Taiwan.

This book provides an in-depth analysis of one the of most popular medicinal plants-Echinacea a species that is native to only the US and Canada. There are nine Echinacea species and several roots and above-ground portions of these showy wildflowers have been used in herbal medicine as an immune stimulant and to reduce one's chances of catching a cold. Considerable medical research supports these claims. The most popular species and the primary one wild-harvested is the one native to the Great Plains, Echinacea angustifolia. It has a long history of use, including being both historically and currently the most widely-used medicinal plant by any of the Great Plains Native Americans. The importance of this species is described by the editor with a few key contributors chosen to relate the important facets of the story of this interesting plant: Echinacea's biology, ecology, medicinal uses, markets, production and harvest, along with population biology, legal protections, ethnobotany, and history. The US Forest Service has expressed concern about the conservation status of Echinacea species on their lands, especially on the National Grasslands and National Forest units in the northern Great Plains. Overall, the future status of Echinacea, as an important medicinal plant and in the wild is not grim, but this book provides a clear perspective of why both cultivated and wild-harvested Echinacea will continue to be available to consumers without threatening the remaining populations.

This is the first English-language book dedicated to Brazilian sand flies and their medical importance. No other country has so many species of these haematophagous insects as Brazil and their diversity has reached an astonishing level. The book contains comprehensive chapters, written by Brazilian experts on their regional distribution, their ecology and their importance as vectors of pathogens and parasites. Methods for sampling, processing and preserving phlebotomines are reviewed as are perspectives on surveillance and leishmaniasis vector control. A novel classification is presented whose aim is to help investigators identify the species that they are working with more efficiently.

Natural and constructed wetlands play a very important role within the landscape and their ecological services are highly valuable. Water management, including flood water retention, biomass production, carbon sequestration, wastewater treatment and as a biodiversity source are among the most important ecological services of wetlands. In order to provide these services, wetlands need to be properly evaluated, protected and maintained. This book provides results of the latest research in wetland science around the world. Chapters deal with such topics as the use of constructed wetlands for treatment of various types of wastewater, use of constructed wetlands in agroforestry, wetland hydrology and evapotranspiration, the effect of wetlands on landscape temperature, and chemical properties of wetland soils.

Edited by Jean-Claude Kader and Michel Delseny and supported by an international Editorial Board, " Advances in Botanical Research" publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 48th volume, the series features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This eclectic volume features six reviews on cutting-edge topics of interest to postgraduates and researchers alike.
* Multidisciplinary reviews written from a broad range of scientific perspectives
* For over 30 years, series has enjoyed a reputation for excellence
* Contributors internationally recognized authorities in their respective fields

In any ecosystem, plant and microbe interaction is inevitable. They not only co-exist but also support each other's survival and also provide for sustenance in stressful environment. Agro-ecosystems of many regions around the globe are affected by multi-stress. Major limiting factors affecting the agricultural productivity worldwide are environmental stresses. Apart from decreasing yield they introduce devastating impact on plant growth as well. Plants battle with various kind of stresses with the help of symbiotic association with the microbes in the rhizosphere. Naturally existing plant-microbe interaction facilitates survival of plants under these stressful conditions. Rhizosphere consists of many groups of microbes, plant growth-promoting bacteria (PGPB) is one such group of microbes which assist plants in coping with multiple stresses and in plant growth as well. These microbes help in stress physiology of the plants and can be extremely useful in solving agricultural as well food security problems. The proposed book is split into two parts, with an aim to provide comprehensive description and highlight a holistic approach. It elucidates various mechanisms in rhizosphere of nutrient management, stress tolerance and enhanced crop productivity. The book discusses rhizospheric flora and its importance in enhancement of plant growth, nutrient content, yield of various crops and vegetables as well as soil fertility and health. Both volumes of the book addresses fundamentals, applications as well as research trends and new prospects of agricultural sustainability. Volume 2: Nutrient Management and Crop Improvement, contains chapters which cover a broad overview of plant growth promoting activities of microbes. This proposed book also highlights the contribution of nitrogen, phosphorus, potassium, iron and zinc-solubilizing microbes from rhizospheric soil to develop efficient indigenous microbial consortia to enhance the food and nutritional security. With the given content and layout the proposed book will be an all-inclusive collection of information, which will be useful for students, academicians, researchers working in the field of rhizospheric mechanisms, agricultural microbiology, soil microbiology, biotechnology, agronomy and sustainable agriculture and also for policy makers in the area of food security and sustainable agriculture. It will be of special interest to both academics and professionals working in the fields of microbiology, soil microbiology, biotechnology and agronomy, as well as the plant protection sciences. Timely, this edited and research book provides an essential and comprehensive source of material from basic to advance findings on microbes and their role in agricultural and soil sustainability.

Traditional medicinal knowledge, especially the use of ethnomedicinal plants in developing countries, has been passed down for generations. Today, however, scientists are poised to combine traditional medicinal plants and modern drug discoveries to further develop essential products that have followed the leads of indigenous cures used for centuries. Ethnomedicinal Plant Use and Practice in Traditional Medicine provides emerging research exploring the theoretical and practical aspects of indigenous knowledge and therapeutic potential within ethnobotany. Featuring coverage on a broad range of topics such as drug discovery, traditional knowledge, and herbal medicine, this book is ideally designed for doctors, healers, medical professionals, ethnobotanists, naturalists, academicians, researchers, and students interested in current research on the medical use and applications of natural-based resources.

The common names of plants often cause difficulties for translators or those engaged in international studies. Although used because they are easier for non-scientists to remember than Latin or Linnean names, one species may have several common names or one common name may be used for several species. The problem is greater for weed scientists because the confusion over common names can lead to misunderstandings over control measures or the importance of weed species. The proposal to list the common names of weeds in the European languages was made in 1972 by the Joint Panel of the Evaluation of Herbicides of the European Plant Protection Organisation, and the work continued by the Working Group on Education and Training of the European Weed Research Society. The result of their labours appears in two volumes. The first is Elsevier's Dictionary of Weeds of Western Europe which was published in 1982, since when it has been a valuable source of information on the common names and importance of weed species in the countries of Western Europe. Its companion volume is this new Dictionary of Weeds of Eastern Europe. Although several books exist which give common names of plants, there are none which have the range of languages covered in these two volumes or provide information on the importance of weed species. The new dictionary will undoubtedly prove to be as welcome and as useful as its predecessor to translators, weed/crop protection scientists, botanists, ecologists, and others.

The current scenario of increasing sensitivity towards the sustainable agriculture has given a large space to extensively utilize natural resources that are environmental friendly and are a good replacement of chemicals in agriculture. Application of organic additives in the sustainable disease management can provide new insight in sustenance of plant productivity along with improved host stress tolerance. In the present book we have focussed upon a range of organic strategies to control plant pathogens of wide spectrum in addition to maintaining robust plant health. A detailed account on the application of organic additives has been discussed, irrespective of their origin and nature. In addition, the methods of utilising these organic supplements in the management of plant diseases and promotion of plant yield in more economic way have also been presented with reference to developing, underdeveloped and developed countries. The book has included the works of eminent scholars from across the world thus flashing light on the key literature related to application of organic matters including phytoextracts, chopped leaves, composted organic manures and liquid manures in eco-friendly agriculture. The mechanisms underlying the effectiveness of these organic amendments in promoting plant health has also been presented and discussed in understandable ways.

Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the "Chlamydomonas Sourcebook" which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants.
"This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually."
* Expanded revision of gold standard reference
* Includes latest advances in research, including completion of the genome
* Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields
* Available as part of a 3-Volume Set or sold individually

"Comparative Plant Virology" provides a complete overview of our current knowledge of plant viruses, including background information on plant viruses and up-to-date aspects of virus biology and control. It deals mainly with concepts rather than detail. The focus will be on plant viruses but due to the changing environment of how virology is taught, comparisons will be drawn with viruses of other kingdomes, animals, fungi and bacteria. It has been written for students of plant virology, plant pathology, virology and microbiology who have no previous knowledge of plant viruses or of virology in general.
* Boxes highlight important information such as virus definition and taxonomy
* Includes profiles of 32 plant viruses that feature extensively in the text
* Companion website providing image bank
* Full color throughout

This volume is a collection of detailed protocols describing state-of-art approaches that will facilitate the understanding of protein homeostasis in plant stress responses and development. Plant Proteostasis: Methods and Protocols is broken into four parts focusing on the study of ubiquitin-dependent post-translational modifications, protocols focused on Ubl post-translational modifications, protein homeostasis, and protocols for the in silico analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols aims to address next challenges in agriculture such as precision horticulture.

The book provides basic knowledge in mycorrhizal ecology, knitted with novel conceptual frameworks and contemporary perspectives, especially in the context of global change. In a fast changing world wherein anthropogenic climate change, biological invasions, deforestation, desertification, and frequent droughts have become routine hard realities, the contents of this book urge readers to rethink basic notions of setting and accomplishing objectives in mycorrhizal research to make sense vis-a-vis contemporary challenges. In this book, a global perspective of mycorrhizal diversity and distribution is provided, followed by some insights into the impact of various global change elements such as climate change, plant invasion, and extreme environmental conditions on mycorrhizas and the role of these mutualists in turn to help their host plants to withstand such novel selection pressures. Special attention here is given to the interesting, but largely neglected, topics such as the role of mycorrhizas in ecological restoration of degraded environments and mycorrhizal status of aquatic plants. The basic idea is to unify various topical areas in mycorrhizal science in an integrated framework. This book can be used by the undergraduate and graduate level students studying mycorrhizal symbioses in the context of current ecological applications. The materials in this book will benefit biological scientists actively involved in research on mycorrhizal ecology and global environmental change. Besides, the contents of the book could be of special interest to restoration ecologists and biodiversity managers. "

Volume 3 reviews virtually everything that is known about cell motility and behavior in Chlamydomonas. World experts in each area focus on mitosis and cytokinesis; flagellar assembly and motility; intraflagellar transport; dynein; the structure and function of centrioles/basal bodies and their associated structures; ciliary signaling; mating and gamete fusion; photobehaviors; and Chlamydomonas as a model for understanding human diseases of the cilium. The volume is richly illustrated and is supplemented by a website containing both classic and previously unpublished videos of cell motility in Chlamydomonas. A unique and especially valuable feature is the inclusion of tables listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process. Because Chlamydomonas has been the premier model for investigating the function and behavior of cilia and flagella, the chapters summarize the current state of knowledge in these areas as it applies to all ciliated organisms. Thus, this volume will be an essential source for all students and researchers interested in cell motility.
* Essential reference to a model species for the study of mechanisms of motility in free living cells
* Includes methods for Chlamydomonas motility research.
* Companion Website with videos illustrates the marvels of the Chlamydomonas flagella in action

Plant taxonomy is an ancient discipline facing new challenges with the current availability of a vast array of molecular approaches which allow reliable genealogy-based classifications. Although the primary focus of plant taxonomy is on the delimitation of species, molecular approaches also provide a better understanding of evolutionary processes, a particularly important issue for some taxonomic complex groups."Molecular Plant Taxonomy: Methods and Protocols"describes laboratory protocols based on the use of nucleic acids and chromosomes for plant taxonomy, as well as guidelines for phylogenetic analysis of molecular data. Experts in the field also contribute review and application chapters that will encourage the reader to develop an integrative taxonomy approach, combining nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play.Written in the successful"Methods in Molecular Biology"series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, "Molecular Plant Taxonomy: Methods and Protocols"seeks to provide conceptual as well as technical guidelines to plant taxonomists and geneticists."

This volume presents a collection of tools currently used for the characterization of rust, the host plant wheat, and their interactions. This book is divided into five parts: Parts I and II discuss advanced techniques for characterizing rust pathogens in rust surveillance, genotyping, and molecular pathogenicity; Part III describes protocols for genetic analysis of rust resistance; Part IV covers methods on rust resistance gene cloning; and Part V talks about the isolation and screening of bacterial endophytes as biocontrol agents for rust disease management. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Wheat Rust Disease: Methods and Protocols is a valuable resource for both established and novel wheat rust researchers and also the plant science and microbial research community.

Chloroplasts are vital for life as we know it. At the leaf cell level, it is common knowledge that a chloroplast interacts with its surroundings - but this knowledge is often limited to the benefits of oxygenic photosynthesis and that chloroplasts provide reduced carbon, nitrogen and sulphur. This book presents the intricate interplay between chloroplasts and their immediate and more distant environments. The topic is explored in chapters covering aspects of evolution, the chloroplast/cytoplasm barrier, transport, division, motility and bidirectional signalling. Taken together, the contributed chapters provide an exciting insight into the complexity of how chloroplast functions are related to cellular and plant-level functions. The recent rapid advances in the presented research areas, largely made possible by the development of molecular techniques and genetic screens of an increasing number of plant model systems, make this interaction a topical issue.