A great fascination for biologists, the study of embryo development provides indispensable information concerning the origins of the various forms and structures that make up an organism, and our ever-increasing knowledge gained through the study of plant embryology promises to lead to the development of numerous useful applications. In Plant Embryo Culture: Methods and Protocols, expert researchers from the field provide a ready source of information for culturing zygotic embryos for different types of studies, both theoretical and practical. The book's main sections examine a wide range of related topics, including the culture of zygotic embryos for developmental studies, the application of embryo culture techniques focusing on embryo rescue methods, cryopreservation of zygotic embryos, the use of zygotic embryos as explants for somatic embryogenesis and organogenesis, as well as transformation protocols using zygotic embryos as starting material. Written in the highly successful Methods in Molecular Biology (TM) series format, the detailed chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital notes on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Plant Embryo Culture: Methods and Protocols serves as a key reference that can be used by scientists of all backgrounds to help develop their own customized methods for many different species and for a variety of purposes.

Forests are restless. When a tree dies or a new one sprouts, the forest that includes it shifts. When new trees sprout in the same direction, the whole forest begins to migrate, sometimes at astonishing rates. Today, however, an array of obstacles-humans felling trees by the billions, invasive pests transported through global trade-threaten to overwhelm these vital movements. Worst of all, the climate is changing faster than ever before and forests are struggling to keep up. A deft blend of science reporting and travel writing, The Journeys of Trees explores the evolving movements of forests by focusing on five trees: giant sequoia, ash, black spruce, Florida torreya and Monterey pine. Zach St George visits these trees in forests across continents, finding sequoias losing their needles in California, fossil records showing the paths of ancient forests in Alaska, domesticated pines in New Zealand and new sprouts of blight-resistant American chestnuts in New Hampshire. Everywhere he goes, St George meets lively people on conservation's front lines, from an ecologist studying droughts to an evolutionary evangelist with plans to save a dying species. He treks through the woods with activists, biologists and foresters, each with their own role to play in the fight for the uncertain future of our environment. An eye-opening investigation into forest migration past and present, The Journeys of Trees examines how we can all help our trees, and our planet, survive and thrive.

This volume provides a collection of protocols aimed toward the study of ethylene signaling in plants. Ethylene Signaling: Methods and Protocols is divided into three sections: ethylene biosynthesis, the signal transduction pathway, and the diverse ethylene responses of dicots and monocots. The chapters in section one discuss techniques for the measurement of activities related to the biosynthetic enzymes ACC synthase and ACC oxidase, the levels of ethylene synthesized by plants, and the treatment of plants with exogenous ethylene. Section two focuses on the analysis of the new membrane-associated proteins involved in the initial perception and transduction of the ethylene signal, such as ethylene receptors, CTR1, and EIN2. The third section covers assays applicable to dicots and monocots, including methods related to the roles of ethylene in germination, growth, abscission, abiotic stress, and defense. Section three also includes information on Arabidopsis mutants and the variety of chemical inhibitors that affect ethylene responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and comprehensive, Ethylene Signaling: Methods and Protocols is a valuable resource for both experienced and beginner researchers with prior experience in the study of ethylene signaling and for those who are just entering this exciting research field.

Optical screening of excessive and potentially harmful solar radiation is an important photoprotective mechanism, though it has received much less attention in comparison with other systems preventing photooxidative damage to photoautotrophic organisms. This photoprotection in the form of screening appears to be especially important for juvenile and senescing plants as well as under environmental stresses-i.e. in situations where the efficiency of enzymatic ROS elimination, DNA repair and other classical' photoprotective systems could be impaired. This book represents an attempt to develop an integral view of optical screening-based photoprotection in microalgae and higher plants. Towards this end, the key groups of pigments involved in the screening of ultraviolet and visible components of solar radiation in microalgae and higher plants, and the patterns of their accumulation and distribution within plant cells and tissues, are described. Special attention is paid to the manifestations of screening pigment accumulation in the optical spectra of plants. It is also demonstrated that understanding these effects and their relationships to screening pigments' makeup and spectroscopy in plants provides valuable insights into the state of plants' long-term photoacclimation, as well as ample opportunities for the non-destructive quantification of screening pigments and the assessment of the efficiency of photoprotection providing by these pigments in situ.

Plant cells house highly dynamic cytoskeletal networks of microtubules and actin microfilaments. They constantly undergo remodeling to fulfill their roles in supporting cell division, enlargement, and differentiation. Following early studies on structural aspects of the networks, recent breakthroughs have connected them with more and more intracellular events essential for plant growth and development. Advanced technologies in cell biology (live-cell imaging in particular), molecular genetics, genomics, and proteomics have revolutionized this field of study. Stories summarized in this book may inspire enthusiastic scientists to pursue new directions toward understanding functions of the plant cytoskeleton.

The Plant Cytoskeleton is divided into three sections: 1) Molecular Basis of the Plant Cytoskeleton; 2) Cytoskeletal Reorganization in Plant Cell Division; and 3) The Cytoskeleton in Plant Growth and Development.

This book is aimed at serving as a resource for anyone who wishes to learn about the plant cytoskeleton beyond ordinary textbooks. "

This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.

Early anthropological evidence for plant use as medicine is 60,000 years old as reported from the Neanderthal grave in Iraq. The importance of plants as medicine is further supported by archeological evidence from Asia and the Middle East. Today, around 1.4 billion people in South Asia alone have no access to modern health care, and rely instead on traditional medicine to alleviate various symptoms. On a global basis, approximately 50 to 80 thousand plant species are used either natively or as pharmaceutical derivatives for life-threatening conditions that include diabetes, hypertension and cancers. As the demand for plant-based medicine rises, there is an unmet need to investigate the quality, safety and efficacy of these herbals by the "scientific methods". Current research on drug discovery from medicinal plants involves a multifaceted approach combining botanical, phytochemical, analytical, and molecular techniques. For instance, high throughput robotic screens have been developed by industry; it is now possible to carry out 50,000 tests per day in the search for compounds which act on a key enzyme or a subset of receptors. This and other bioassays thus offer hope that one may eventually identify compounds for treating a variety of diseases or conditions. However, drug development from natural products is not without its problems. Frequent challenges encountered include the procurement of raw materials, the selection and implementation of appropriate high-throughput bioassays, and the scaling-up of preparative procedures. Research scientists should therefore arm themselves with the right tools and knowledge in order to harness the vast potentials of plant-based therapeutics. The main objective of Plant and Human Health is to serve as a comprehensive guide for this endeavor. Volume 1 highlights how humans from specific areas or cultures use indigenous plants. Despite technological developments, herbal drugs still occupy a preferential place in a majority of the population in the third world and have slowly taken roots as alternative medicine in the West. The integration of modern science with traditional uses of herbal drugs is important for our understanding of this ethnobotanical relationship. Volume 2 deals with the phytochemical and molecular characterization of herbal medicine. Specifically, It will focus on the secondary metabolic compounds which afford protection against diseases. Lastly, Volume 3 focuses on the physiological mechanisms by which the active ingredients of medicinal plants serve to improve human health. Together this three-volume collection intends to bridge the gap for herbalists, traditional and modern medical practitioners, and students and researchers in botany and horticulture.

It was around 1970, I had just completed a 5-year breeding project aiming at fxing fower colour in gerbera progenies: white, yellow, pink, and red; colour homogeneity was sound, but size and shape still required some improvement. The problem was defnitely resolved by Murashige and Skoog, USA who published a reliable protocol for gerbera micropro- gation. In short, my gerbera seed lines were immediately rendered obsolete by this e- cient cloning system, able to produce millions of plants of a matchless and previously unknown homogeneity, the uniformity of fower shape and colour being the basic requi- ments for the market. The success of micropropagation resulted in a tremendous growth in gerbera fower production worldwide, and this species conquered a leading place in the foriculture industry. This personal experience stresses the impact of micropropagation on the genetic improvement research strategies in ornamentals. Micropropagation has become "in- sive", especially in ornamental plant material issues. Today, hundreds of protocols exist; however, only a modest percentage of them are exploited economically. Thus, only micropropagation of plants with a high market price range, like orchids for instance, has proved cost-effective and achieved great success. Micropropagation is a labour-intensive system: hand-power is estimated to rep- sent 60-70% of total costs. This explains the outsourcing of the major labs in developing countries where labour is cheaper. Nevertheless, certain industrial protocols remain a proprietary technology of leading labs, mostly western, with the exception of Japan and Taiwan.

This book provides an in-depth analysis of one the of most popular medicinal plants-Echinacea a species that is native to only the US and Canada. There are nine Echinacea species and several roots and above-ground portions of these showy wildflowers have been used in herbal medicine as an immune stimulant and to reduce one's chances of catching a cold. Considerable medical research supports these claims. The most popular species and the primary one wild-harvested is the one native to the Great Plains, Echinacea angustifolia. It has a long history of use, including being both historically and currently the most widely-used medicinal plant by any of the Great Plains Native Americans. The importance of this species is described by the editor with a few key contributors chosen to relate the important facets of the story of this interesting plant: Echinacea's biology, ecology, medicinal uses, markets, production and harvest, along with population biology, legal protections, ethnobotany, and history. The US Forest Service has expressed concern about the conservation status of Echinacea species on their lands, especially on the National Grasslands and National Forest units in the northern Great Plains. Overall, the future status of Echinacea, as an important medicinal plant and in the wild is not grim, but this book provides a clear perspective of why both cultivated and wild-harvested Echinacea will continue to be available to consumers without threatening the remaining populations.

This is the first English-language book dedicated to Brazilian sand flies and their medical importance. No other country has so many species of these haematophagous insects as Brazil and their diversity has reached an astonishing level. The book contains comprehensive chapters, written by Brazilian experts on their regional distribution, their ecology and their importance as vectors of pathogens and parasites. Methods for sampling, processing and preserving phlebotomines are reviewed as are perspectives on surveillance and leishmaniasis vector control. A novel classification is presented whose aim is to help investigators identify the species that they are working with more efficiently.

Natural and constructed wetlands play a very important role within the landscape and their ecological services are highly valuable. Water management, including flood water retention, biomass production, carbon sequestration, wastewater treatment and as a biodiversity source are among the most important ecological services of wetlands. In order to provide these services, wetlands need to be properly evaluated, protected and maintained. This book provides results of the latest research in wetland science around the world. Chapters deal with such topics as the use of constructed wetlands for treatment of various types of wastewater, use of constructed wetlands in agroforestry, wetland hydrology and evapotranspiration, the effect of wetlands on landscape temperature, and chemical properties of wetland soils.

Edited by Jean-Claude Kader and Michel Delseny and supported by an international Editorial Board, " Advances in Botanical Research" publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 48th volume, the series features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This eclectic volume features six reviews on cutting-edge topics of interest to postgraduates and researchers alike.
* Multidisciplinary reviews written from a broad range of scientific perspectives
* For over 30 years, series has enjoyed a reputation for excellence
* Contributors internationally recognized authorities in their respective fields

In any ecosystem, plant and microbe interaction is inevitable. They not only co-exist but also support each other's survival and also provide for sustenance in stressful environment. Agro-ecosystems of many regions around the globe are affected by multi-stress. Major limiting factors affecting the agricultural productivity worldwide are environmental stresses. Apart from decreasing yield they introduce devastating impact on plant growth as well. Plants battle with various kind of stresses with the help of symbiotic association with the microbes in the rhizosphere. Naturally existing plant-microbe interaction facilitates survival of plants under these stressful conditions. Rhizosphere consists of many groups of microbes, plant growth-promoting bacteria (PGPB) is one such group of microbes which assist plants in coping with multiple stresses and in plant growth as well. These microbes help in stress physiology of the plants and can be extremely useful in solving agricultural as well food security problems. The proposed book is split into two parts, with an aim to provide comprehensive description and highlight a holistic approach. It elucidates various mechanisms in rhizosphere of nutrient management, stress tolerance and enhanced crop productivity. The book discusses rhizospheric flora and its importance in enhancement of plant growth, nutrient content, yield of various crops and vegetables as well as soil fertility and health. Both volumes of the book addresses fundamentals, applications as well as research trends and new prospects of agricultural sustainability. Volume 2: Nutrient Management and Crop Improvement, contains chapters which cover a broad overview of plant growth promoting activities of microbes. This proposed book also highlights the contribution of nitrogen, phosphorus, potassium, iron and zinc-solubilizing microbes from rhizospheric soil to develop efficient indigenous microbial consortia to enhance the food and nutritional security. With the given content and layout the proposed book will be an all-inclusive collection of information, which will be useful for students, academicians, researchers working in the field of rhizospheric mechanisms, agricultural microbiology, soil microbiology, biotechnology, agronomy and sustainable agriculture and also for policy makers in the area of food security and sustainable agriculture. It will be of special interest to both academics and professionals working in the fields of microbiology, soil microbiology, biotechnology and agronomy, as well as the plant protection sciences. Timely, this edited and research book provides an essential and comprehensive source of material from basic to advance findings on microbes and their role in agricultural and soil sustainability.

Plant Ecology & Conservation is an introduction to the world of plant ecology. It includes the main areas of current research including ideas about plant populations, nutrition and plant community ecology and has a particular emphasis on the interactions of plants with animals, fungi and microorganisms whose important is being increasingly demonstrated. With the world's environmental problems having such a high profile, the book focusses on the human impact on the world's plant species. Conservation of the terrestrial world starts with plants as they form the basis of all ecosystems on land. We can only understand how best to conserve the world's biodiversity with an understanding of the central role of plant ecology. This theme runs throughout with numerous examples of the disruption of ecosystems by human activity emphasising the connection between plant ecology and conservation. Key Features: Boxes present case studies, important statistics and interesting asides Full-colour photos depict key species and habitats and superb line drawings illustrate many concepts Important data are presented in Tables and Figures throughout Each chapter has Key Concepts and review questions to test a reader's grasp of the content Key References and Further Reading are given for each chapter to point the reader towards the most important and influential literature Jargon is kept to a minimum and a full Glossary of all technical terms is presented The book is aimed primarily at undergraduate and graduate students in any aspect of ecology or plant science. It should also appeal to anyone interested in how plants function and are concerned about what is needed for the conservation of the world's ecosystems.

Traditional medicinal knowledge, especially the use of ethnomedicinal plants in developing countries, has been passed down for generations. Today, however, scientists are poised to combine traditional medicinal plants and modern drug discoveries to further develop essential products that have followed the leads of indigenous cures used for centuries. Ethnomedicinal Plant Use and Practice in Traditional Medicine provides emerging research exploring the theoretical and practical aspects of indigenous knowledge and therapeutic potential within ethnobotany. Featuring coverage on a broad range of topics such as drug discovery, traditional knowledge, and herbal medicine, this book is ideally designed for doctors, healers, medical professionals, ethnobotanists, naturalists, academicians, researchers, and students interested in current research on the medical use and applications of natural-based resources.

The common names of plants often cause difficulties for translators or those engaged in international studies. Although used because they are easier for non-scientists to remember than Latin or Linnean names, one species may have several common names or one common name may be used for several species. The problem is greater for weed scientists because the confusion over common names can lead to misunderstandings over control measures or the importance of weed species. The proposal to list the common names of weeds in the European languages was made in 1972 by the Joint Panel of the Evaluation of Herbicides of the European Plant Protection Organisation, and the work continued by the Working Group on Education and Training of the European Weed Research Society. The result of their labours appears in two volumes. The first is Elsevier's Dictionary of Weeds of Western Europe which was published in 1982, since when it has been a valuable source of information on the common names and importance of weed species in the countries of Western Europe. Its companion volume is this new Dictionary of Weeds of Eastern Europe. Although several books exist which give common names of plants, there are none which have the range of languages covered in these two volumes or provide information on the importance of weed species. The new dictionary will undoubtedly prove to be as welcome and as useful as its predecessor to translators, weed/crop protection scientists, botanists, ecologists, and others.

The current scenario of increasing sensitivity towards the sustainable agriculture has given a large space to extensively utilize natural resources that are environmental friendly and are a good replacement of chemicals in agriculture. Application of organic additives in the sustainable disease management can provide new insight in sustenance of plant productivity along with improved host stress tolerance. In the present book we have focussed upon a range of organic strategies to control plant pathogens of wide spectrum in addition to maintaining robust plant health. A detailed account on the application of organic additives has been discussed, irrespective of their origin and nature. In addition, the methods of utilising these organic supplements in the management of plant diseases and promotion of plant yield in more economic way have also been presented with reference to developing, underdeveloped and developed countries. The book has included the works of eminent scholars from across the world thus flashing light on the key literature related to application of organic matters including phytoextracts, chopped leaves, composted organic manures and liquid manures in eco-friendly agriculture. The mechanisms underlying the effectiveness of these organic amendments in promoting plant health has also been presented and discussed in understandable ways.

Dr. Harris has played a major role in the development of this organism as a model system. Her previous version of the "Chlamydomonas Sourcebook" which published in 1989, has been a classic in the field and is considered required reading for anyone working with this organism. This latest edition has been expanded to include three volumes providing molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Methods for Chlamydomonas research and best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, are included. Additions to this volume also include help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants.
"This volume is part of a 3-Volume Set (ISBN: 978-0-12-370873-1) and is also sold individually."
* Expanded revision of gold standard reference
* Includes latest advances in research, including completion of the genome
* Provides broad perspective with studies in cell and molecular biology, genetics, plant physiology and related fields
* Available as part of a 3-Volume Set or sold individually

"Comparative Plant Virology" provides a complete overview of our current knowledge of plant viruses, including background information on plant viruses and up-to-date aspects of virus biology and control. It deals mainly with concepts rather than detail. The focus will be on plant viruses but due to the changing environment of how virology is taught, comparisons will be drawn with viruses of other kingdomes, animals, fungi and bacteria. It has been written for students of plant virology, plant pathology, virology and microbiology who have no previous knowledge of plant viruses or of virology in general.
* Boxes highlight important information such as virus definition and taxonomy
* Includes profiles of 32 plant viruses that feature extensively in the text
* Companion website providing image bank
* Full color throughout

This volume is a collection of detailed protocols describing state-of-art approaches that will facilitate the understanding of protein homeostasis in plant stress responses and development. Plant Proteostasis: Methods and Protocols is broken into four parts focusing on the study of ubiquitin-dependent post-translational modifications, protocols focused on Ubl post-translational modifications, protein homeostasis, and protocols for the in silico analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Proteostasis: Methods and Protocols aims to address next challenges in agriculture such as precision horticulture.

The book provides basic knowledge in mycorrhizal ecology, knitted with novel conceptual frameworks and contemporary perspectives, especially in the context of global change. In a fast changing world wherein anthropogenic climate change, biological invasions, deforestation, desertification, and frequent droughts have become routine hard realities, the contents of this book urge readers to rethink basic notions of setting and accomplishing objectives in mycorrhizal research to make sense vis-a-vis contemporary challenges. In this book, a global perspective of mycorrhizal diversity and distribution is provided, followed by some insights into the impact of various global change elements such as climate change, plant invasion, and extreme environmental conditions on mycorrhizas and the role of these mutualists in turn to help their host plants to withstand such novel selection pressures. Special attention here is given to the interesting, but largely neglected, topics such as the role of mycorrhizas in ecological restoration of degraded environments and mycorrhizal status of aquatic plants. The basic idea is to unify various topical areas in mycorrhizal science in an integrated framework. This book can be used by the undergraduate and graduate level students studying mycorrhizal symbioses in the context of current ecological applications. The materials in this book will benefit biological scientists actively involved in research on mycorrhizal ecology and global environmental change. Besides, the contents of the book could be of special interest to restoration ecologists and biodiversity managers. "