Applications of microbial nanotechnology are currently emerging with new areas being explored. Biosynthesis of nanomaterials by microorganisms is a recently attracting interest as a new, exciting approach towards the development of 'greener' nanomanufacturing compared to traditional chemical and physical approaches. This book will cover recent advances of microbial nanotechnology in agriculture, industry, and health sectors.

Bioactive natural compounds have gained attention in recent years due to their potential health benefits, including reducing the risk of diabetes, cancer, and cardiovascular diseases. These benefits derive from bioactive compounds' anti-tumor, anti-inflammatory, anti-oxidative, anti-hypertensive and anti-hyperlipidemic activities, which serve in addition to their basic nutritional functions. Over the last decade, researchers have investigated the health impact of bioactive compounds in detail, and the development of food applications has attracted great interest. Consumer demand has surged for functional foods (nutraceuticals), superfoods, and tailor-made foods, generated by supplementing traditional food products with bioactive ingredients. Food Bioactives and Health offers comprehensive coverage of the properties and health effects of food bioactives in view of new trends in processing, food science and food technology. Starting with the metabolic characteristics of polyphenols, glucosinolates, and other food bioactives, the text then dives into their impact on human health and recent applications in the world of food technology. For food scientists, food technologists, and product developers looking to understand the role of food bioactives in health and develop applications in personalized nutrition, functional foods and nutraceuticals, Food Bioactives and Health serves as a one-stop reference.

This book presents a comprehensive overview of DNA barcoding and molecular phylogeny, along with a number of case studies. It discusses a number of areas where DNA barcoding can be applied, such as clinical microbiology, especially in relation to infection management; DNA database management; and plant -animal interactions, and also presents valuable information on the DNA barcoding and molecular phylogeny of microbes, algae, elasmobranchs, fishes, birds and ruminant mammals. Furthermore it features unique case studies describing DNA barcoding of reptiles dwelling in Saudi Arabian deserts, genetic variation studies in both wild and hatchery populations of Anabas testudineus, DNA barcoding and molecular phylogeny of Ichthyoplankton and juvenile fishes of Kuantan River in Malaysia, and barcoding and molecular phylogenetic analysis of indigenous bacteria from fishes dwelling in a tropical tidal river. Moreover, since prompt identification and management of invasive species is vital to prevent economic and ecological loss, the book includes a chapter on DNA barcoding of invasive species. Given its scope, this book will appeal not only to researchers, teachers and students around the globe, but also to general readers.

Freshwater Microbiology: Perspectives of Bacterial Dynamics in Lake Ecosystems provides a comprehensive and systematic analysis of microbial ecology in lakes. It offers basic information on how well the bacterial community composition varies along the spatio-temporal and trophic gradients along with the evaluation of the bioindicator species of bacteria so as to act as a key to predict the trophic status of lake ecosystems. The book helps to identify the factors of potential importance in structuring the bacterial communities in lakes as it delves into the dynamics and diversity of bacterial community composition in relation to various water quality parameters. It helps to identify the possibility of bioremediation plans and devising future policy decisions, with better conservation and management practices.

This thesis demonstrates a technology that enables pipetting-free high-throughput screening (HTS) on a miniaturized platform, eliminating the need for thousands of one-by-one pipetting and conventional liquid handling systems. This platform enhances accessibility to HTS and enables HTS to be used in small-to-medium scale laboratories. In addition, it allows large-scale combinatorial screening with a small number of valuable cells, such as patients' primary cancer cells. This technique will have a high impact for widespread use of HTS in the era of personalized medicine. In this thesis, the author firstly describes the need and concept of 'partipetting' for pipetting-free HTS platform. It is realized by the one-step pipetting and self-assembly of encoded drug-laden microparticles (DLPs) on the microwells. Next, the technical implementations required for the platform demonstration are described. It includes preparation of encoded DLPs, plastic chip fabrication, and realization of automated system. Lastly, screening of sequential drug combinations using this platform is demonstrated. This shows the potential of the proposed technology for various applications.

Bacteriology Methods for the Study of Infectious Diseases provides knowledge, understanding and experience of contemporary, robust methodologies for studies into the pathogenicity and virulence of human/animal bacterial pathogens. This book presents contemporary, yet widely utilized methodologies, for the study of pathogenicity and virulence in bacterial pathogens of human and/or animal origin. Protocols are clearly outlined, with lists of required equipment and reagents, alongside underpinning theory. This text will provide undergraduate and postgraduate students with practical guidance for dissertation projects with protocols for individual project ideas that can be developed further, hence a starting point for additional literature searches is also provided.

Achieving environmental sustainability with rapid industrialization is a major challenge of current scenario worldwide. As globally evident, industries are the key economic drivers, but are also the major polluters as untreated/partially treated effluents discharged from the industries is usually thrown into the aquatic resources and also dumped unattended. Industrial effluents are considered as the major sources of environmental pollution as these contains highly toxic and hazardous pollutants, which reaches far off areas due to the medium of dispersion and thus, create ecological nuisance and health hazards in living beings. Hence, there is an urgent to find ecofriendly solution to deal with industrial waste, and develop sustainable methods for treating/detoxifying wastewater before its release into the environment. Being a low cost and eco-friendly clean technology, bioremediation can be a sustainable alternative to conventional remediation technologies for treatment and management of industrial wastes to protect public health and environment. Therefore, this book (Volume I) covers the bioremediation of different industrial wastes viz. tannery wastewater, pulp and paper mill wastewater, distillery wastewater, acid mine tailing wastes, and many more; which are lacking in a comprehensive manner in previous literature at one place. A separate chapter dedicated to major industries and type of waste produced by them is also included. This book will appeal to students, researchers, scientists, industry persons and professionals in field of microbiology, biotechnology, environmental sciences, eco-toxicology, environmental remediation and waste management and other relevant areas, who aspire to work on the biodegradation and bioremediation of industrial wastes for environmental safety.

Smart Bioremediation Technologies: Microbial Enzymes provides insights into the complex behavior of enzymes and identifies metabolites and their degradation pathways. It will help readers work towards solutions for sustainable medicine and environmental pollution. The book highlights the microbial enzymes that have replaced many plant and animal enzymes, also presenting their applications in varying industries, including pharmaceuticals, genetic engineering, biofuels, diagnostics and therapy. In addition, new methods, including genomics andmetagenomics, are being employed for the discovery of new enzymes from microbes. This book brings all of these topics together, representing the first resource on how to solve problems in bioremediation.

This volume comprehensively reviews recent advances in our understanding of the diversity of microbes in various types of terrestrial ecosystems, such as caves, deserts and cultivated fields. It is written by leading experts, and highlights the culturable microbes identified using conventional approaches, as well as non-culturable ones unveiled with metagenomic and microbiomic approaches. It discusses the role of microbes in ecosystem sustainability and their potential biotechnological applications. The book further discusses the diversity and utility of ectomycorrhizal and entomopathogenic fungi and yeasts that dwell on grapes, it examines the biotechnological applications of specific microbes such as lichens, xylan- and cellulose-saccharifying bacteria and archaea, chitinolytic bacteria, methanogenic archaea and pathogenic yeasts.

This book sheds light on the major functions of microbial communities in aquaculture ecosystems, showing that by recycling nutrients, degrading organic matter and preventing disease outbreaks, a variety of microbes are truly beneficial to a wide range of aquaculture industries. It discusses how deteriorating environmental quality enables some microbial strains to trigger disease, describes the development of highly sustainable tools to improve water quality, and identifies crucial factors that endanger microbial homeostasis in aquaculture ecosystems. The book also covers post-antibiotic approaches for preventing and treating opportunistic microbial infections based on harnessing environmental and fish-associated microbial communities. Furthermore, it explores how manipulating and engineering these complex microbial communities using bio-agents such as probiotics, phages, natural nutritional additives, or with fine-tuned biofilters will open the door for new ways to develop a more sustainable and cost-effective aquaculture industry. Including an accessible presentation of modern high-throughput sequencing technology to identify host-microbial interactions in aquaculture ecosystems, this book is a valuable resource for scientists, aquaculture and fishery experts, sustainability enthusiasts and scholars in the areas of biology and marine agriculture.

This book presents a comprehensive review of renewable energy-based sustainable drying techniques for developing countries. Aspiring towards a world with zero food waste, the book has provided discussion on sustainable drying techniques in terms of energy efficiency. The socio-economic condition of each developing country is unique; therefore, has specific technological requirements. As such, the book presents discussions on food waste scenario around the world, the socio-economic status of developing countries and their correlation with food. The book gives an overview of the quality aspects of drying, along with the required energy and time to retain these features. Additionally, a method of selecting drying techniques for developing countries, taking the cost and safety factor into consideration, has been discussed extensively Also, the renewable and non-renewable energy resources of low income, lower-middle income, middle income, and high-income developing countries have been analyzed and presented. The book also highlights the available drying techniques that are currently being practiced by the consumers and industries of developing countries. The book recommends ten sustainable drying technologies for the developing countries and describes their working principle. Discussion on potential challenges for sustainable drying technology adoption is also presented. The book presents up-to-date research on sustainable drying techniques and their impact on developing countries to reduce food waste. Food waste is not only a humanitarian concern but also a threat to environmental sustainability. Currently, one-third of all produced food is being wasted, when nearly 805 million people - including children remain undernourished on a daily basis. In an effort to solve this crisis, a number of food preservations techniques are being practiced in food supply chain. Drying is one such preservation technique that prevents microbial proliferation, slows enzymatic reaction and preserves the physio-chemical properties of food. Albeit, drying is an effective means of food preservation; it is also highly energy-intensive. Developing countries do not have sufficient energy and financial resources to adopt conventional (expensive and high energy) drying techniques. As such, this is the first reference work dedicated to discussing the prospects and challenges of sustainable (renewable energy based and inexpensive) drying techniques for developing countries in order to reduce food waste. Sustainable food drying techniques in developing countries: Prospects and Challenges is a singular work in the field of food preservation and affordable drying technology.

Rhinovirus Infections: Rethinking the Impact on Human Health and Disease provides a timely review of the impact of rhinovirus infections on human health. It identifies disease mechanisms relating to the virus, human host and environmental factors. This viewpoint allows us to look forward to the development of treatments for a virus for which treatment options are currently non-existent. By providing detailed insights into this virus, its host and the environmental factors that play into rhinovirus induced diseases, this book explains disease mechanisms and summarizes existing and developing therapeutic approaches for better research, diagnosis and potential treatments.

The generation of well-defined nanoparticles of excellent size and shape involves physical and chemical methodologies that are complicated, expensive, and produce hazardous toxic waste that is harmful to the environment and to human health. In order to combat the disadvantages of these methods, scientists have created "the biological method," a new synthetic methodology that serves as a proper alternative to physical and chemical methodologies because of its easy utility, low cost, rapid synthesis, controlled size characteristics, controlled toxicity, and eco-friendliness. Nanobiotechnology is the science in which living matter can be manipulated and exploited to produce materials within the nano-scale. It is a multidisciplinary field of science framed by biology, chemistry, engineering, materials, and life sciences. Different biological entities can be exploited to yield biologically synthesized nanomaterials including bacteria, actinomycetes, yeast, fungi, viruses, algae, plant extracts, and agro-industrial waste extracts. This book represents a comprehensive review concerning the state of the art in nanobiotechnology, emphasizing the use of diverse biological entities in the science, and its versatile applications. It describes currently existing methodology with the latest published references, and provides safety information. It serves as the ideal guide for scientists interested in exploring nanobiotechnology.

Over 50% of known flaviviruses have been associated with human disease. The "Flavivirus" genus constitutes some of the most serious human pathogens including Japanese encephalitis, dengue and yellow fever. Flaviviruses are known for their complex life cycles and epidemic spread, and are considered a globally-emergent viral threat.
Detection, Diagnosis and Vaccine Development, the third volume of The Flaviviruses details the current status of technologies for detection and differentiation of these viruses, their use in surveillance and outbreak investigation, and also reviews the latest clinical research.
* Comprehensive approach to the scientific disciplines needed to unravle the complexities of virus-host interactions
* Descibes the technologies that have contributed to our current knowledge about the Flaviviruses
* Identifies the major problems faced in understanding the virus-host interactins that result in disease
* An exhaustive compendium of current and past knowledge on the Flavivirus family

This volume provides a clear understanding of how microbes, following their degradative processes, contribute maximally to the benefit of mankind through biotransformations of waste materials as well as a wide variety of health-risk compounds.

The book contains twenty four chapters contributed by leading scientists from different parts of the world, covering various aspects of bioremediation of xenobiotics such as toxic, carcinogenic, teratogenic, and mutagenic compounds, which include halogenated aromatics, derivatives of heavy metals, microbial toxins, tannins, dyes, sulfur compounds of coal and petroleum and pesticides. The bioremediation of agricultural residue, industrial as well as municipal wastes, fuel oils, lubricants, natural rubber products, and other synthetic polymers, which pollute the environment substantially, also constitutes an important component of the book. All biotechnological aspects of microbial transformations pertaining to biodegradation/bioremediation of hazardous wastes, ranging from screening methods for microbes with degradative potential, processes of degradation, strain improvement for enhanced biodegradation and elimination of xenobiotics of health and environmental concern have been dealt with.

The book intends to widen the scope of Applied Microbiology and Biotechnology in general and biotransformations in particular. It will provide an opportunity for scientists in the areas of biochemistry, food industry, environmental science and engineering and their implications in technologically feasible, environment friendly and economically viable bioremediation options. Also, it forms an interface between agro-industrial establishments and the academic world and will generate new thought provoking ideas for scientists of future generations for the safeguard of both human and animal health as well as the environment.

Salmonellae are important pathogens, responsible for an estimated one million deaths and 100 million human infections annually. Their genomes are mosaic puzzles, results of lateral transfer events that occur within a stable genetic background. Extraordinary diversity of host ranges and pathogenicity traits between different strains are the consequence of both specific genome insertions/deletions and minute changes in genome composition. Genomic information decoded from a multitude of different Salmonella strains and new dramatic insights into pathogenic processes emphasize the fact that Salmonella research is currently at a very exciting juncture. In addition to their fascinating resilience in both the environment and eukaryotic hosts, Salmonella prefer tumors over any other location within the human host (by a factor of 1000 or more). This ability could propel Salmonella into future use as a therapeutic delivery agent to control and/or cure cancers. In this book, internationally accla

The Pigments from Microalgae Handbook presents the current state of knowledge on pigment production using microalgae-based processes, and covers both the scientific fundamentals of this technology and its practical applications. It addresses biology, chemistry, biochemistry, analysis and engineering aspects, as well as applications of natural pigments in photosynthetic organisms. The book also describes the analytical procedures associated with the characterization of pigments and the engineering aspects of microalgal pigment production. It considers the three major classes of pigments(chlorophylls, carotenoids and phycobiliproteins) produced and surveys the main commercial applications of these chemicals. The book offers a valuable source of information for industrial researchers and practitioners in industrial biotechnology, as it covers various engineering aspects of microalgal pigment production, such as bioreactors and bioprocesses, industrial extraction processes, and the bioeconomy of production including life-cycle assessment. The book will also be of interest to undergraduate and graduate students of biochemistry, food chemistry, and industrial microbiology.

This book addresses multiple aspects of biological clocks in prokaryotes. The first part of the book deals with the circadian clock system in cyanobacteria, i.e. the pioneer of bacterial clocks. Starting with the history and background of cyanobacteria and circadian rhythms in microorganisms, the topics range from the molecular basis, structure and evolution of the circadian clock to modelling approaches, Kai systems in cyanobacteria and biotechnological applications. In the second part, emergent timekeeping properties of bacteria in microbiomes and bacteria other than cyanobacteria are discussed. Since the discovery of circadian rhythms in cyanobacteria in the late 1980s, the field has exploded with new information. The cyanobacterial model system for studying circadian rhythms (Synechococcus elongatus), has allowed a detailed genetic dissection of the bacterial clock due to state-of-the-art methods in molecular, structural, and evolutionary biology. Cutting-edge research spanning from cyanobacteria and circadian phenomena in other kinds of bacteria, to microbiomes has now given the field another major boost. This book is aimed at junior and senior researchers alike. Students or researchers new to the field of biological clocks in prokaryotes will get a comprehensive overview, while more experienced researchers will get an update on the latest developments.

Fungal growths affect both human and animal well-being. Many natural pathogens of laboratory animals alter host physiology, rendering the host unsuitable for experimental uses. While the number and prevalence of few pathogens have declined considerably, many still turn up in laboratory animals and represent unwanted variables in research. Investigators using laboratory animals in biomedical experimentation should be aware of the profound effects that many of these agents can have on research. What does the future hold regarding the natural pathogens of laboratory animals? The selection of an animal model must carefully address issues of the type of human disease to mimic, the parameters to follow, and the collection of the appropriate data to answer those questions being asked. Overall, animal models of fungal infection will continue to deepen our understanding of how these infections occur. This book provides a valuable source of information to biological and biomedical scientists and to clinical and doctoral researchers working in the area of fungal infections and diseases of laboratory animal species.

Cyanobacteria constitute the most widely distributed group of photosynthetic prokaryotes found in almost all realms of the earth and play an important role in Earth's nitrogen and carbon cycle. The gradual transformation from reducing atmosphere to oxidizing atmosphere was a turning point in the evolutionary history of the earth and made conditions for present life forms possible. Cyanobacteria: From Basic Science to Applications is the first reference volume that comprehensively discusses all aspects of cyanobacteria, including the diverse mechanisms of cyanobacteria for the advancement of cyanobacterial abilities, towards higher biofuel productivity, enhanced tolerance to environmental stress and bioactive compounds and potential for biofertilizers.

Plant endophytes are a potential source for the production of bioactive compounds that can fight against devastating diseases in both plants and humans. Among these endophytic microorganisms, endophytic fungi are one of the dominant group of microorganisms with a potential role in plant growth promotion and the discovery of noble bioactive natural products. Endophytic fungi possess several bioactivities like anticancer, antimicrobial, insecticidal, plant growth stimulants, crop protection, phytoremediation, etc. Presence of modular biosynthetic genes clusters like PKS and NRPS in several endophytic fungi underscores the need to understand and explore such organisms. This volume presents and demonstrates the applied aspects of endophytic fungi. Practical applications of such endophytes are discussed in detail, including studies in pharmaceutical development and agricultural management of important microbial diseases. The beneficial effects that endophytic fungi provide to host plants-enhancing growth, increasing fitness, strengthening tolerance to abiotic and biotic stresses through secondary metabolites-are also discussed. The reader is provided with a comprehensive and detailed understanding of such relationships between endophytic fungi and their host.

This book gathers selected studies on the industrial applications of glycoside hydrolases (GHs), presenting an updated classification of these enzymes, and discussing their structure, mechanisms, and various approaches to improve their catalytic efficiency. Further, it explains the various industrial applications of glycoside hydrolases in food, effluent treatment, biofuel production, and the paper and pulp industries. Lastly, the book provides a comparative analysis of glycoside hydrolases and discusses the role of metagenomics in the discovery of industrially important enzymes. As such it is a thought-provoking, instructive and informative resource for biochemists, enzymologists, molecular biologists and bioprocess technologists.

This book introduces readers to basic studies on and applied techniques involving lactic acid bacteria, including their bioengineering and industrial applications. It summarizes recent biotechnological advances in lactic acid bacteria for food and health, and provides detailed information on the applications of these bacteria in fermented foods. Accordingly, it offers a valuable resource for researchers and graduate students in the fields of food microbiology, bioengineering, fermentation engineering, food science, nutrition and health.