This volume focuses on mitochondrial RNA metabolism, emphasizing recent discoveries and technological advances in this fast moving area that increase our understanding of mitochondrial gene function. Topics addressed include the interplay of mitochondria with the nucleus and cytosol, structure-function connections, and relevance to human disease. Mitochondria are the powerhouses of the cell, and a great deal is known about mitochondrial energy metabolism. Less well known is the plethora of amazing mechanisms that have evolved to control expression of mitochondrial genomes. Several RNA processes and machineries in protozoa, plants, flies and humans are discussed, including: transcription and RNA polymerase mechanism; tRNA processing of 5' and 3' ends; mRNA maturation by nucleotide insertion/deletion editing and by RNA splicing; mRNA stability; and RNA import. Specialized factors and ribonucleoproteins (RNPs) examined include pentatricopeptide repeat (PPR) proteins, RNase P, polymerases, helicases, nucleases, editing and repair enzymes. Remarkable features of these processes and factors are either not found outside mitochondria, differ substantially among eukaryotic lineages, or are unique in biology.

This book focuses on the application of nanotechnology in medicine and drug delivery, including diagnosis and therapy. Nanomedicine can contribute to the development of a personalized medicine both for diagnosis and therapy. By interacting with biological molecules at nanoscale level, nanotechnology opens up an immense field of research and applications. Interactions between artificial molecular assemblies or nanodevices and biomolecules can be understood both in the extracellular medium and inside human cells. Operating at nanoscale allows exploitation of physical properties different from those observed at microscale, such as the volume to surface area ratio. A number of clinical applications of nanobiotechnology, such as disease diagnosis, target-specific drug delivery, and molecular imaging are being investigated. Some promising new products are also undergoing clinical trials. Such advanced applications of this approach to biological systems will undoubtedly transform the foundations of diagnosis, treatment, and prevention of disease in the future. Nanomedicine sales reached $16 billion in 2015, with a minimum of $3.8 billion in nanotechnology R&D being invested each year. Global funding for emerging nanotechnology increased by 45% per year in recent years, with product sales exceeding $1 trillion in 2013. As the nanomedicine industry continues to grow, it is expected to have a significant impact on the global economy. This book provides clear, colorful and simple illustrations, tables, and case studies to clearly convey the content to a general audience and reader. This book also discusses the development of nanobiomaterials from biogenic (biological sources) systems for healthcare and disease therapies. This book, therefore, is useful for researchers and academicians in the fields of nanotechnology, medicine, nano-biotechnology and pharmacology.

Bifidobacteria are Gram-positive anaerobic bacteria, found naturally in the gut of humans and other mammals. They are widely used as probiotic organisms in a vast array of formulations for the prevention, alleviation, and treatment of many intestinal disorders. However, bifidobacteria are fastidious microorganisms and are difficult to study in the laboratory, so until recently, understanding of their genetics lagged behind that of other high GC content Gram-positive bacteria. The application of modern whole genome approaches to bifidobacteria research has changed all of this, permitting the accumulation of an impressive amount of data, something that could not have been foreseen a few years ago. This book brings together the expertise and enthusiasm of the leading bifidobacteria researchers from around the world to provide a state-of-the art overview of the molecular biology and genomics of this exciting and important microbial genus. Topics include: ecology, genomics, comparative geno

The Microbiology of Central Nervous System Infections, Volume 3, discusses modern approaches to the diagnosis, treatment and prophylaxis of central nervous system (CNS) infections. This new release is divided into five sections that cover treatment strategies, imaging, molecular diagnosis, management of CNS infections with metal nanoparticles, and prophylaxis of CNS infections, including bacterial, viral and fungal infections. The last section contains a chapter on transmissible spongiform encephalopathies and modern trends in its diagnosis and treatment. University teachers, medical practitioners, graduate and postgraduate students, researchers in microbiology, and those in the pharmaceutical and laboratory diagnostic industries will find the book very important.

This book gives a comprehensive overview on the various aspects of Trichoderma, a filamentous fungus ubiquitously present in soil. Topics addressed are the biology, diversity, taxonomy, ecology, biotechnology and cultivation of Trichoderma, to just name a few. Basic as well as applied aspects are covered and a special focus is given on use of Trichoderma in agriculture and beyond. Trichoderma species are widely distributed throughout the world in soil, rotting plant material, and wood. Although they are often considered as a contaminants, Trichoderma species are also known for their ability to act as biocontrol agents against various plant pathogens and plant diseases, and also as biostimulants promoting plant growth. The contents of this book will be of particular interest to, agricultural scientists, biotechnologists, plant pathologists, mycologists, and microbiologists, students, extension workers, policy makers and other stakeholders.

This book covers current developments in membrane-based technologies for the successful recovery of food bioactive ingredients and molecules. Chapters explore emerging technologies, such as microfiltration, ultrafiltration, nanofiltration, and membrane distillation, for the selective concentration and food ingredients from food by-products, as well as techniques, such as pervaporation, for the selective separation and recovery of aroma compounds. The text provides one of the first examinations of other membrane-based technologies, such as liquid membranes (microemulsions), membrane distillation (MD) and pervaporation (PV), as thermal driven membrane processes. The separation of metabolites from microalgae and fermentation broths using membrane technologies is also covered. Researchers in food science, pharmaceutics and biotechnology looking to stay up-to-date on bioactive recovery, as well as membranologists exploring new applications for membrane-based technologies, will find this text a useful resource.

This updated second edition covers the molecular biology, genome engineering tools and comprehensive analysis techniques for Corynebacterium glutamicum. Aside from modern omics-based approaches, the authors also focus on cell physiology, including cell division, central carbon metabolic pathways, and the respiratory chain. Readers will learn how primary mechanisms like energy metabolism can be applied in processes like biorefinery. Newly added topics include cell envelope structures and aromatic compound metabolism in C. glutamicum. These chapters will be particularly useful for those interested in the microbial production of commodity chemicals, fuels, and proteins. Corynebacteriacea are already some of the most important industrial microorganisms. Understanding the cell physiology of C. glutamicum will help manufacturers to increase their product range and productivity through efficient metabolic engineering.

Environmental sustainability is one of the biggest issues faced by the mankind. Rapid & rampant industrialization has put great pressure on the natural resources. To make our planet a sustainable ecosystem, habitable for future generations & provide equal opportunity for all the living creatures we not only need to make corrections but also remediate the polluted natural resources. The low-input biotechnological techniques involving microbes and plants can provide the solution for resurrecting the ecosystems. Bioremediation and biodegradation can be used to improve the conditions of polluted soil and water bodies. Green energy involving biofuels have to replace the fossil fuels to combat pollution & global warming. Biological alternatives (bioinoculants) have to replace harmful chemicals for maintaining sustainability of agro-ecosystems. The book will cover the latest developments in environmental biotech so as to use in clearing and maintaining the ecosystems for sustainable future.

This book reviews the wide range of products and applications of solid state fermentation as well as the development of this cultivation technology over the last years. In this book, readers will also learn about the challenges of solid state fermentation, including process management, reactor design, scale-up and the formation of process-specific products. Solid fermentation is a traditional cultivation technique of food technology and involves all cultivations of microorganisms on a solid substrate without free liquid phase. In the course of development of Biotechnology it was replaced by liquid cultivation mainly in the western countries. Over the past few years, solid-state fermentation is now becoming more important and has moved more back into focus. Especially, it is suitable for the cultivation of filamentous organisms, like ascomycetes and basidiomycetes, but also for various yeasts and bacteria. The products and applications of solid-state fermentation are as diverse as the microorganisms. They range from enzyme production to the production of antibiotics and pigments to the use in environmental technology and energy production.

This book addresses all the major mechanisms by which endophytes are thought to impact plant growth and health. A unique aspect of this publication is that it is multidisciplinary, covering plant microbiology, plant physiology, fungal and bacterial endophytes, plant biochemistry, and genomics. Just as research on the mammalian microbiome has demonstrated its importance for overall health of the host, the plant microbiota is essential for plant health in natural environments. Endophytes, the microorganisms living fully within plants, can provide a multitude of benefits to the host including N-fixation, P solubilization, increased photosynthetic efficiency and water use efficiency, stress tolerance, pathogen resistance, and overall increased growth and health. A variety of culturable endophytes have been isolated and shown to be mutualistic symbionts with a broad range of plant species. These studies point to the functional importance of the microbiota of plants and suggest the potential for tailoring plant microbiota for improved vigor and yields with reduced inputs. This review covers the major benefits of microbial endophytes to plants and discusses the implications of using symbiosis as an alternative to chemical inputs for agriculture, forestry, and bioenergy.

This volume presents state-of-the-art protocols for key experiments that have revolutionized our understanding of the bacterial nucleoid. This book is divided into five parts: Part I introduces molecular genetic methods to study bacterial nucleoids; Part II highlights the study of bacterial nucleoid with whole genome analysis method; Part III discusses molecular biology methods to study nucleoid structuring factors; Part IV looks at imaging bacterial nucleoid; and Part V explores biophysics of the bacterial nucleoid. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, The Bacterial Nucleoid: Methods and Protocols is a valuable resource that provides a wealth of new information about this chromosome.

This book gathers the latest findings on the microbial ecology of saline habitats, plant-microbe interactions under saline conditions, and saline soil reclamation for agricultural use. The content is divided into four main parts: Part I outlines the definition of salinity, its genesis and impacts, and microbial diversity in saline habitats. Part II deals with impact of salinity on microbial and plant life/health. Part III highlights plant - microbe interactions in saline environments, and Part IV describes strategies for mitigation and reclamation of saline soils. The salinization of arable land is steadily increasing in many parts of the world. An excessive concentration of soluble salts (salinity) in soils or irrigation water adversely affects plant growth and survival. This problem is exacerbated in arid and semiarid areas due to their low precipitation and high evaporation rates. In turn, poor management practices and policies for using river water for the irrigation of agriculture crops often lead to the secondary salinization of soils. Considering the growing demands of a constantly expanding population, understanding the microbial ecology and interactions under saline conditions and their implications for sustainable agriculture is of utmost importance. Providing both an essential review of the status quo and a future outlook, this book represents a valuable asset for researchers, environmentalists and students working in microbiology and agriculture. .

This book gathers the latest insights into soil health and its sustainability, providing an up-to-date overview of the various aspects of soil quality and fertility management, e.g., plant-microbe interactions to maintain soil health; and the use of algal, fungal and bacterial fertilizers and earthworms for sustainable soil health and agricultural production. It first discusses the past, present, and future scenarios of soil health, and then explores factors influencing soil health, as well as the consequences of degradation of soil health for sustainable agriculture. Lastly it highlights solutions to improve and maintain soil health so as to achieve greater productivity and sustainability without damaging the soil system or the environment. Soil health is defined as the capacity of a soil to function within ecosystem frontiers, to sustain biological productivity, to maintain environmental quality and to promote plant, animal and human health. Soil health is established through the interactions of physical, chemical and biological properties, e.g., soil texture, soil structure, and soil organisms. Healthy soil provides adequate levels of macro- and micronutrients to plants and contains sufficient populations of soil microorganisms. As a result of the increasingly intensified agriculture over the past few decades, soils are now showing symptoms of exhaustion and stagnating or declining crop yields. Exploring these developments as well as possible solutions based on holistic and sustainable approaches, this book is a valuable resource for researchers in the area of soil and environmental science, agronomy, agriculture, as well as students in the field of botany, ecology and microbiology.

This book focuses on the regulation of transcription and translation in Archaea and arising insights into the evolution of RNA processing pathways. From synthesis to degradation and the implications of gene expression, it presents the current state of knowledge on archaeal RNA biology in 13 chapters. Topics covered include the modification and maturation of RNAs, the function of small non-coding RNAs and the CRISPR-Cas defense system. While Archaea have long been considered exotic microbial extremophiles, they are now increasingly being recognized as important model microorganisms for the study of molecular mechanisms conserved across the three domains of life, and with regard to the relevance of similarities and differences to eukaryotes and bacteria. This unique book offers a valuable resource for all readers interested in the regulation of gene expression in Archaea and RNA metabolism in general.

Brazilian Microbiome: Current status and perspectives unites a set of distinguished investigators conducting microbiome research and builds a comprehensive reference book with up-to-date information regarding the Brazilian microbiome studies and trends. It covers terrestrial and host associated microbiomes, unveiling biological, biotechnological and technical aspects of research. This book is devoted to students and professionals interested in learning techniques for microbiome surveys, including culture-independent approaches, and to better understand the biology of microorganisms in nature, with emphasis on the Brazilian microbiomes.

Biodegradation has been the subject of active concern for the past 40 years. Recently, the field has expanded to encompass a wide variety of chemicals, a broad array of issues, and the development of the new bioremediation industry. This book presents the basic principles of biodegradation and shows how these principles relate to bioremediation. Authored by a world-renowned environmental microbiologist, Biodegradation and Bioremediation presents microbiological, chemical, toxicological, environmental, engineering, and technological aspects of the subject.
* Praise for the First Edition
* "Serves an important function in presenting a complete picture of biodegradation in the environment. I t] provides biologists, chemists and engineers interested in this process with information complementary to their own fields and thus belongs on the shelf of everybody active in the study of biodegradation and bioremediation."
* --JOURNAL OF CONTAMINANT HYDROLOGY
* "A compendious account of the field in a form accessible to microbiologists, chemists, toxicologists and environmental scientists and engineers. His book succeeds admirably...Well indexed, its diagrams large and clear....It will serve as an excellent text for advanced undergraduate and postgraduate courses and as an invaluable source of reference to all those with a research interest in this expanding field."
* --SOCIETY FOR GENERAL MICROBIOLOGY QUARTERLY
* "The book could serve as a text for an upper-level or graduate class in the principles of biodegradation and bioremediation for microbiologists and environmental engineers. In summary, Alexander has prepared a cogent and well-organized review of the principles of biodegradation in a book that should prove useful to a great many people."
* --JOURNAL OF ENVIRONMENTAL QUALITY
* "Provides a unity of style, and generally, an excellent intellectual flow between topics....Alexander does a spectacular job of addressing the kinetics of degradation reactions--the discussion is one of the best I have seen, especially for those not well versed in mathematics."
* --BIOSCIENCE
* "Written by a very knowledgeable scientist...A very timely book...Recommended as a valuable resource for environmental scientists, engineers, or other persons with an interest in the use of organisms for environmental decontamination and a need for better insight into the principles underlying bioremediation."
* --SOIL SCIENCE

This book covers both the molecular basics of fungal stress response strategies as well as biotechnological applications thereof. The complex regulatory mechanisms of stress response pathways are presented in a concise and well-readable manner. Also, light will be shed on the interconnection of pathways responding to different types of stress. Profound knowledge of stress responses in yeast and filamentous fungi is crucial for further optimization of industrial processes. Applications are manifold, for example in fungicide development, for improving the resistance of crop plants to fungal pathogens, but also in medicine to help curing fungal infections. The book targets researchers from academia and industry, as well as graduate students interested in microbiology, mycology and biomedicine.

Aimed at students, researchers, nutritionists, and developers in food technology, this research text addresses the nascent field of metabiotics. Metabiotics are products based on components of cells, metabolites, and signaling molecules released by probiotic strains, engineered to optimize host-specific physiological functions in a way that traditional probiotics cannot. This book examines the history, processes, design, classifications, and functions of metabiotics. It includes an overview of the composition and function of the gut microbiota, and discusses development of target-specific metabiotics. Further coverage includes comparisons to traditional probiotics, as well as probiotic safety and side-effects. Metabiotics: Present State, Challenges and Perspectives provides a complete history and understanding of this new field, the next phase of the probiotic industry.

This volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.

Extensively revised, accessible, and up-to-date—the new edition of Tate’s classic soil microbiology text Now in its second edition, Soil Microbiology offers students a broad knowledge of the behavior and function of microbes in soil—all the essentials they will need in order to address the long-term stewardship of the earth’s soil resources. Designed for use as the core text for microbiology courses in the soil science curriculum, it explores the tremendous diversity of life found in soil ecosystems. With its amplified focus on the reclamation of contaminated and damaged soils, greenhouse gas production, and the sustainability of soil ecosystems, this book is an ideal resource for upper-level undergraduates and graduate students, as well as a useful reference for professionals in soil and environmental science. Soil Microbiology, Second Edition features:

• A focus on new advances as well as environmental science aspects of soil microbiology
• Completely new chapters on the biological diversity of soil ecosystems, soil remediation, and soil systems management
• A strong emphasis on research in real-world settings as well as theoretical concerns

A proper understanding of the diversity, systematics, and nomenclature of microbes is increasingly important in many branches of biological science. The molecular approach to phylogenetic analysis - pioneered by Carl Woese in the 1970s and leading to the three-domain model (Archaea, Bacteria, Eucarya) - has revolutionized our thinking about evolution in the microbial world. The technological innovation of modern molecular biology and the rapid advancement in computational science have led to a flood of nucleic acid sequence information, bioinformatic tools, and phylogenetic inference methods. Phylogenetic analysis has long played a central role in microbiology and the emerging fields of comparative genomics and phylogenomics require substantial knowledge and understanding of phylogenetic analysis and computational methods. In this book, leading scientists from around the world explore current concepts in molecular phylogeny and their application with respect to microorganisms. The authors describe the different approaches applied today to elucidate the molecular phylogeny of prokaryotes (and eukaryotic protists) and review current phylogenetic methods, techniques, and software tools. Topics covered include: a historical overview, computational tools, multilocus sequence analysis, 16S rRNA phylogenetic trees, rooting of the universal tree of life, applications of conserved indels, lateral gene transfer, endosymbiosis, and the evolution of plastids.

This is a completely revised edition, including new material, from 'Culture Media for Food Microbiology' by J.E.L. Corry et al., published in Progress in Industrial Microbiology, Volume 34, Second Impression 1999.

Written by the Working Party on Culture Media, of the International Committee on Food Microbiology and Hygiene, this is a handy reference for microbiologists wanting to know which media to use for the detection of various groups of microbes in food, and how to check their performance.

The first part comprises reviews, written by international experts, of the media designed to isolate the major groups of microbes important in food spoilage, food fermentations or food-borne disease. The history and rationale of the selective agents, and the indicator systems are considered, as well as the relative merits of the various media. The second part contains monographs on approximately 90 of the most useful media.

The first edition of this book has been frequently quoted in standard methods, especially those published by the International Standards Organisation (ISO) and the European Standards Organisation (CEN), as well as in the manuals of companies manufacturing microbiological media. In this second edition, almost all of the reviews have been completely rewritten, and the remainder revised. Approximately twelve monographs have been added and a few deleted. This book will be useful to anyone working in laboratories examining food - industrial, contract, medical, academic or public analyst, as well as other microbiologists, working in the pharmaceutical, cosmetic and clinical (medical and veterinary) areas - particularly with respect to quality assurance of media and methods in relation to laboratory accreditation.

The origin of life is one of the biggest unsolved scientific questions. This book deals with the formation and first steps of the chemical evolution of nucleic acids, including the chemical roots behind the origin of their components from the simplest sources in a geochemical context. Chemical evolution encompasses the chemical processes and interactions conducive to self-assembly and supramolecular organization, leading to an increase of complexity and the emergence of life. The book starts with a personal account of the pioneering work of Stanley Miller and Jeffrey Bada on the Chemistry of Origins of Life and how the development of organic chemistry beginning in the 19th century led to the emergence of the field of prebiotic chemistry, situated at the frontier between organic, geo- and biochemistry. It then continues reviewing in tutorial manner current central topics regarding the organization of nucleic acids: the origin of nucleobases and nucleosides, their phosphorylation and polymerization and ultimately, their self-assembly and supramolecular organization at the inception of life.

Zika Virus provides an authoritative account of one of most fascinating viruses of the 21st century, covering all the main points. It includes coverage of clinical manifestations, such as fever and fatigue, but also delves into neurological manifestations like acute demyelinating neuropathy. In addition, the book discusses new evidence that suggests that Zika fever in pregnant women can cause abnormal brain development in fetuses by mother-to-child transmission. The Zika virus infection has become one of the first where women are actively discouraged from getting pregnant. Readers will find this book to be a comprehensive resource on the topic.

This book discusses how aquatic microbial communities develop interactive metabolic coordination both within and between species to optimize their energetics. It explains that microbial community structuration often includes functional stratification among a multitude of organisms that variously exist either suspended in the water, lodged in sediments, or bound to one another as biofilms on solid surfaces. The authors describe techniques that can be used for preparing and distributing microbiologically safe drinking water, which presents the challenge of successfully removing the pathogenic members of the aquatic microbial community and then safely delivering that water to consumers. Drinking water distribution systems have their own microbial ecology, which we must both understand and control in order to maintain the safety of the water supply. Since studying aquatic microorganisms often entails identifying them, the book also discusses techniques for successfully isolating and cultivating bacteria. As such, it appeals to microbiologists, microbial ecologists and water quality scientists.