This volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.

This book covers both the molecular basics of fungal stress response strategies as well as biotechnological applications thereof. The complex regulatory mechanisms of stress response pathways are presented in a concise and well-readable manner. Also, light will be shed on the interconnection of pathways responding to different types of stress. Profound knowledge of stress responses in yeast and filamentous fungi is crucial for further optimization of industrial processes. Applications are manifold, for example in fungicide development, for improving the resistance of crop plants to fungal pathogens, but also in medicine to help curing fungal infections. The book targets researchers from academia and industry, as well as graduate students interested in microbiology, mycology and biomedicine.

Sterol Regulation of Ion Channels, Volume 80, the latest in the Current Topics in Membranes series provides outstanding content to the scientific community on relevant topics that relate to the broad field of membranes. Topics of note in this new release include Membrane structure and general mechanisms of sterol regulation of ion channels, the Regulation of ion channels by sterols as boundary lipids, the Differential effects of sterols on ion channels: specific vs. non-specific interactions, the Structural determinants of cholesterol-ion channels interactions, and the Regulation of Ca2+-sensitive K+ channels by cholesterol and bile acids via distinct channel subunits and sites, amongst other specialized chapters. The serial is targeted towards scientists and researchers in biochemistry and molecular and cellular biology, providing the necessary membrane research to assist them in discovering the current state of a particular field. The authors and editors associated with the Current Topics in Membranes series are recognized as world-renowned scientists in their respective fields, making CTM one of the premier serials on membranes

This book reviews the wide range of products and applications of solid state fermentation as well as the development of this cultivation technology over the last years. In this book, readers will also learn about the challenges of solid state fermentation, including process management, reactor design, scale-up and the formation of process-specific products. Solid fermentation is a traditional cultivation technique of food technology and involves all cultivations of microorganisms on a solid substrate without free liquid phase. In the course of development of Biotechnology it was replaced by liquid cultivation mainly in the western countries. Over the past few years, solid-state fermentation is now becoming more important and has moved more back into focus. Especially, it is suitable for the cultivation of filamentous organisms, like ascomycetes and basidiomycetes, but also for various yeasts and bacteria. The products and applications of solid-state fermentation are as diverse as the microorganisms. They range from enzyme production to the production of antibiotics and pigments to the use in environmental technology and energy production.

Myxomycetes: Biology, Systematics, Biogeography, and Ecology is a comprehensive overview of the body of accumulated knowledge that now exists on myxomycetes. Its broad scope takes an integrated approach to the knowledge of this organismal group, considering a number of important aspects of their genetics and molecular phylogeny. It also treats myxomycetes as a distinct group from fungi, and includes molecular information that discusses the systematics and evolutionary pathways of the group. Additionally, biomedical and engineering applicability is discussed, thus expanding the audience and use of the book in a multidisciplinary context. The book provides an authoritative resource for students, researchers and educators interested in the fields of protistology, microbial ecology, molecular microbiology, biogeography, mycology, biodiversity, and evolutionary biology, and will also interest the amateur naturalist and biologist.

Advances in Microbial Physiology, Volume 71, continues the long tradition of topical, important, cutting-edge reviews in microbiology. The book contains updates in the field, with comprehensive chapters covering The Microbiology of Ruthenium Complexes, The role of plant growth-promoting bacteria in metal phytoremediation, the Mechanism and Role of Globin Coupled Sensor Signaling, Cytochrome bd and gaseous ligands in bacterial physiology, and Haem-Based Sensors of O2: Lessons and Perspectives.

The origin of life is one of the biggest unsolved scientific questions. This book deals with the formation and first steps of the chemical evolution of nucleic acids, including the chemical roots behind the origin of their components from the simplest sources in a geochemical context. Chemical evolution encompasses the chemical processes and interactions conducive to self-assembly and supramolecular organization, leading to an increase of complexity and the emergence of life. The book starts with a personal account of the pioneering work of Stanley Miller and Jeffrey Bada on the Chemistry of Origins of Life and how the development of organic chemistry beginning in the 19th century led to the emergence of the field of prebiotic chemistry, situated at the frontier between organic, geo- and biochemistry. It then continues reviewing in tutorial manner current central topics regarding the organization of nucleic acids: the origin of nucleobases and nucleosides, their phosphorylation and polymerization and ultimately, their self-assembly and supramolecular organization at the inception of life.

This book gathers the latest findings on the microbial ecology of saline habitats, plant-microbe interactions under saline conditions, and saline soil reclamation for agricultural use. The content is divided into four main parts: Part I outlines the definition of salinity, its genesis and impacts, and microbial diversity in saline habitats. Part II deals with impact of salinity on microbial and plant life/health. Part III highlights plant - microbe interactions in saline environments, and Part IV describes strategies for mitigation and reclamation of saline soils. The salinization of arable land is steadily increasing in many parts of the world. An excessive concentration of soluble salts (salinity) in soils or irrigation water adversely affects plant growth and survival. This problem is exacerbated in arid and semiarid areas due to their low precipitation and high evaporation rates. In turn, poor management practices and policies for using river water for the irrigation of agriculture crops often lead to the secondary salinization of soils. Considering the growing demands of a constantly expanding population, understanding the microbial ecology and interactions under saline conditions and their implications for sustainable agriculture is of utmost importance. Providing both an essential review of the status quo and a future outlook, this book represents a valuable asset for researchers, environmentalists and students working in microbiology and agriculture. .

Ebola's Curse: 2013-2016 Outbreak in West Africa is about hemorrhagic fever viruses, especially Ebola, its initial origin in central Africa 1976, its unprecedented appearance in West Africa in 2013. The book records in sequence and detective style how the initial outbreak of Ebola from the index case in rural Guinea traveled to Sierra Leone, the work and fate of those working in the Kenema Government Hospital (KGH) isolation ward in Sierra Leone. The book provides vignettes of the three main players involved with Ebola at KGH, Sheik Khan, Pardis Sabeti, and Robert Garry. Khan was the head of the unit, declared a national hero by his Sierra Leone government. He died fighting Ebola and was/is recognized in the USA by American societies by awards created for his historic work and death. Pardis Sabeti, a geneticist from Harvard and Broad MIT Institute, who was honored as a "Scientist of the Year" by Time Magazine and the Smithsonian Institute. Robert Garry, head of the operation to fight hemorrhagic fevers and Ebola, shuttled between Tulane University, KGH, and The White House to make aware through the press and others the dilemma and tragedy that was unfolding, and the need to obtain additional medical and health care support and supplies. Sabeti and Garry currently work with Oldstone on Ebola at KGH and thus personal communication and knowledge was/is available to the author for the book.

Viral Proteases and Their Inhibitors provides a thorough examination of viral proteases from their molecular components, to therapeutic applications. As information on three dimensional structures and biological functions of these viral proteases become known, unexpected protein folds and unique mechanisms of proteolysis are realized. This book investigates how this facilitates the design and development of potent antiviral agents used against life-threatening viruses. Users will find descriptions of each virus that detail the structure and function of viral proteases, discuss the design and development of inhibitors, and analyze the structure-activity relationships of inhibitors. This book is ideal biochemists, virologists and those working on antiviral agents.

Microbial infections still represent one of the major causes of mortality and morbidity worldwide. Irrational usage of antimicrobials has lead to increased resistance, causing clinical, social and economical disabilities. Therefore, one of the major challenges of scientists is to develop novel alternative methods to handle infections and reduce resistance and other side effects produced by the actual therapies. The aim of this book is to offer a perspective on novel approaches to handle infections by using naturally-derived products in order to modulate the virulence of pathogens, without the risk of developing resistance. We intend to highlight the utility of microbial, vegetal and animal-derived compounds with potential antimicrobial activity by exploiting their effect on microbial virulence. Furthermore, this book aims to reveal the potential to assimilate recent bio-technological findings, like the usage of nanotechnology as efficient shuttles for stabilizing, improved targeting and the controlled release of natural products in order to efficiently fight infections.

Advances in Microbial Physiology, Volume 70 continues the long tradition of topical, important, cutting-edge reviews in microbiology with this new volume covering a variety of topics, including Bacterial Hemoprotein Sensors of NO: H-NOX and NosP, Manganese in Marine Microbiology, Nutritional Immunity and Fungal Pathogenesis: The Struggle for Micronutrients at the Host-Pathogen Interface, Metal-Based Combinations that Target Protein Synthesis by Fungi, Transition Metal Homeostasis in Streptococcus Pyogenes and Streptococcus Pneumoniae, Copper and Antibiotics: Discovery, Modes of Action, and Opportunities for Medicinal Applications, Metal Resistance and Its Association with Antibiotic Resistance, and The Role of Intermetal Competition and Mis-Metalation in Metal Toxicity.

This book gives an overview on techniques and future perspectives of various aspects of waste biomass management. It also presents the economic and environmental evaluation, and also the monetary value-benefits and sustainability of the different processes. Recycling processes of lignocellulosic biomass from palm oil mill waste are covered, as well as from sugar industry waste and agriculture waste. It also includes thermal and non-thermal technologies for resource recovery from waste biomass. Challenges in the reuse and recycling of waste biomass are discussed, i.e., the hygienic safety in biomass management and bioremediation technologies for conversion into valuable products. The book is aiming at scientists, researchers and students alike, who are working in the research areas pertaining to waste management

This book presents a summary of terrestrial microbial processes, which are a key factor in supporting healthy life on our planet. The authors explain how microorganisms maintain the soil ecosystem through recycling carbon and nitrogen and then provide insights into how soil microbiology processes integrate into ecosystem science, helping to achieve successful bioremediation as well as safe and effective operation of landfills, and enabling the design of composting processes that reduce the amount of waste that is placed in landfills. The book also explores the effect of human land use, including restoration on soil microbial communities and the response of wetland microbial communities to anthropogenic pollutants. Lastly it discusses the role of fungi in causing damaging, and often lethal, infectious diseases in plants and animals.

This book discusses how aquatic microbial communities develop interactive metabolic coordination both within and between species to optimize their energetics. It explains that microbial community structuration often includes functional stratification among a multitude of organisms that variously exist either suspended in the water, lodged in sediments, or bound to one another as biofilms on solid surfaces. The authors describe techniques that can be used for preparing and distributing microbiologically safe drinking water, which presents the challenge of successfully removing the pathogenic members of the aquatic microbial community and then safely delivering that water to consumers. Drinking water distribution systems have their own microbial ecology, which we must both understand and control in order to maintain the safety of the water supply. Since studying aquatic microorganisms often entails identifying them, the book also discusses techniques for successfully isolating and cultivating bacteria. As such, it appeals to microbiologists, microbial ecologists and water quality scientists.

Antimicrobial Stewardship (AMS), Volume Two includes the experience of ESGAP workshops and courses on antibiotic stewardship since 2012. It combines clinical and laboratory information about AMS, with a focus on human medicine. The ESCMID study group on antibiotic policies (ESGAP) is one of the most productive groups in the field, organizing courses and workshops. This book is an ideal tool for the participants of these workshops. With short chapters (around 1500 words) written on different topics, the authors insisted on the following points: A 'hands on', practical approach, tips to increase success, a description of the most common mistakes, a global picture (out- and inpatient settings, all countries) and a short list of 10-20 landmark references.

This book provides authentic and comprehensive information on the concepts, methods, functional details and applications of nano-emulsions. Following an introduction to the applications of nanotechnology in the development of foods, it elaborates on food-grade nano-emulsion and their significance, discusses various techniques and methods for producing food-grade nano-emulsion, and reviews the main ingredient and component of food-grade nano-emulsions. Further, the book includes a critical review of the engineering aspect of fabricating food-grade nano-emulsions and describe recently developed vitamin encapsulated nano-systems. In closing, it discuss the challenges and opportunities of characterizing nano-emulsified systems, the market risks and opportunities of nano-emulsified foods, and packaging techniques and safety issues - including risk identification and risk management - for nano-foods. The book offers a unique guide for scientists and researchers working in this field. It will also help researchers, policymakers, industry personnel, journalists and the general public to understand food nanotechnology in great detail.

This book provides information about the nontarget nature of selected soil enzymes which are implicated in soil fertility and health and the methods for their assay. It also shows how these soil enzymes are affected by two different pesticides, buprofezin and acephate, used both extensively and intensively in modern agriculture.

The study of the molecular events leading to cellular transformation and cancer has progressed significantly in the last decade, and it has become apparent that many genes subject to modification in cancer are, in fact, transcription factors that govern the execution of the genetic programme of the cell. Transcription factors can behave either as oncogenes or as tumour suppressor genes. To date only a limited number of transcription factors have been associated with cancer. This volume deals with several transcription factor families that were first identified in oncogenic retroviruses. Each chapter contains a description of the structure of the transcription factors, the nature of target genes, the regulation of their activities, and an explaination of how they can deregulate cell growth and differentiation. This text should be suitable for the specialist scientist and the advanced student

This book presents a compilation of case studies from different countries on achieving agricultural sustainability. The book stresses that, in order to meet the needs of our rapidly growing population, it is imperative to increase agricultural productivity. If global food production is to keep pace with an increasing population, while formulating new food production strategies for developing countries, the great challenge for modern societies is to boost agricultural productivity. Today, the application of chemicals to enhance plant growth or induced resistance in plants is limited due to the negative effects of chemical treatment and the difficulty of determining the optimal concentrations to benefit the plant. In the search for alternative means to solve these problems, biological applications have been extensively studied. Naturally occurring plant-microbe-environment interactions are utilized in many ways to enhance plant productivity. As such, a greater understanding of how plants and microbes coexist and benefit one another can yield new strategies to improve plant productivity in the most sustainable way. Developing sustainable agricultural practices requires understanding both the basic and applied aspects of agriculturally important microorganisms, with a focus on transforming agricultural systems from being nutrient-deficient to nutrient-rich. This work is divided into two volumes, the aim being to provide a comprehensive description and to highlight a holistic approach, respectively. Taken together, the two volumes address the fundamentals, applications, research trends and new prospects of agricultural sustainability.  Volume one consists of two sections, with the first addressing the role of microbes in sustainability, and the second exploring beneficial soil microbe interaction in several economically important crops. Section I elucidates various mechanisms and beneficial natural processes that enhance soil fertility and create rhizospheric conditions favourable for high fertility and sustainable soil flora. It examines the mechanism of action and importance of rhizobacteria and mycorrhizal associations in soil. In turn, section II presents selected case studies involving economically important crops. This section explains how agriculturally beneficial microbes have been utilized in sustainable cultivation with high productivity. Sustainable food production without degrading the soil and environmental quality is a major priority throughout the world, making this book a timely addition. It offers a comprehensive collection of information that will benefit students and researchers working in the field of rhizospheric mechanisms, agricultural microbiology, biotechnology, agronomy and sustainable agriculture, as well as policymakers in the area of food security and sustainable agriculture.

This book presents the latest results related to photocatalytic inactivation/killing of microorganisms, which is a promising alternative disinfection method that produces less or even no disinfection byproduct. The book is divided into 13 chapters, which introduce readers to the latest developments in the photocatalytic disinfection of microorganisms, examine essential photocatalytic (PC) and photoelectrocatalytic (PEC) disinfection studies, and forecast and make recommendations for the further development of PC and PEC disinfection. Bringing together contributions by various leading research groups worldwide, it offers a valuable resource for researchers and the industry alike, as well as the general public. Taicheng An, PhD, is Chair Professor and Director at the Institute of Environmental Health and Pollution Control, School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou, China. Huijun Zhao, PhD, is Chair Professor and Director at the Centre for Clean Environment and Energy & Griffith School of Environment, Griffith University, Australia. Po Keung Wong, PhD, is a Professor at the School of Life Sciences, the Chinese University of Hong Kong, Hong Kong SAR, China.

This Volume presents methods for analysing and quantifying petroleum, hydrocarbons and lipids, based on their chemical and physical properties as well as their biological effects. It features protocols for extracting hydrocarbons from solid matrices, water and air, and a dedicated chapter focusing on volatile organic compounds. Several approaches for separating and detecting diverse classes of hydrocarbons and lipids are described, including: (tandem) gas chromatography (GC) coupled with mass spectrometry (MS) or flame-ionisation detection, Fourier-transform induction-coupled-resonance MS, and fluorescence-based techniques. The book details high-performance liquid chromatography MS for microbial lipids, as well as a combination of techniques for naphthenic acids. Two chapters focus on quantifying bioavailable hydrocarbon fractions by using cyclodextrin sorbents and bacterial bioreporters, respectively, while a closing chapter explains how compound-specific stable-isotope analysis can be used to measure the fate of hydrocarbons in the environment. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.