The book will address selected topics in postharvest pathology aiming at highlighting recent development in the science, technology and control strategies of postharvest diseases to reduce losses and enhance safety of harvested agricultural products. Topics will include: 1) Introduction: Perspectives and challenges in postharvest pathology 2) Elucidating host-pathogen interactions 3) Next generation technologies for management and detection of postharvest pathogens 4) Food safety in postharvest pathology 5) Alternative postharvest diseases control strategies 6) Chemical control of postharvest diseases

This volume on iron-sulfur proteins includes chapters that describe the initial discovery of iron-sulfur proteins in the 1960s to elucidation of the roles of iron sulfur clusters as prosthetic groups of enzymes, such as the citric acid cycle enzyme, aconitase, and numerous other proteins, ranging from nitrogenase to DNA repair proteins. The capacity of iron sulfur clusters to accept and delocalize single electrons is explained by basic chemical principles, which illustrate why iron sulfur proteins are uniquely suitable for electron transport and other activities. Techniques used for detection and stabilization of iron-sulfur clusters, including EPR and Mossbauer spectroscopies, are discussed because they are important for characterizing unrecognized and elusive iron sulfur proteins. Recent insights into how nitrogenase works have arisen from multiple advances, described here, including studies of high-resolution crystal structures.

Novel Food Fermentation Technologies provides a comprehensive overview of innovations in food fermentation technologies and their application. Current novel technologies for microbial culture production and preservation are covered in detail, as are fermentation techniques for the production of bioactives from various food matrices, including food processing by-products and waste. Readers are provided with a close look at thermal and non-thermal technologies applicable to fermented food products. The text covers immobilization, microencapsulation technologies and novel preservation techniques for cultures in fermentation. In-depth studies of high pressure processing, pulsed electric field, power ultrasound and gamma irradiation in fermentation are provided in addition to novel thermal and non-thermal technologies and process analytical techniques. A wide variety of fermented products are covered, including meat, marine-based, grain-based, dairy and vegetable-based products. Current technologies for extraction of bioactives are examined, as are current innovations in fermented food packaging. Readers are presented with current and future challenges in food fermentation as well. As a comprehensive reference for food fermentation, this work provides up-to-date insights into emerging fermentation technologies which facilitate the processing of wholesome and safe food products.

Every day many people suffer from intestinal diseases. These disorders can result from pathogens like bacteria, fungi, parasites and viruses, but the causes of non-infectious intestinal disorders and colorectal cancers remain to be elucidated. Disturbances to the normal gut flora (the microbiota) are central to the development of many, if not all, of these disorders.

Disturbed gut microbiota is a prelude to public health issues like traveller's-, antibiotic- and "Clostridium difficile"-associated diarrhoea, irritable bowel syndrome, inflammatory bowel disease, and colorectal cancers. This book discusses the way intestinal disorders affect the microbiota, how the disturbed microbiotal balance leads to enteric disorders and the ways to prevent these disorders.

Further his book explores the potential ofprobiotics (live microorganisms that when ingested bring a health benefit) in treating enteric disorders by analysing the probiotic genome through proteomics, metabolomics and functional assays. Discussed is how the ingestion of specific microorganisms repairs the disturbed microbiota and subsequently ameliorates enteric disorders. Finally this book addresses how genetic engineering and biotechnology will contribute to the development of effective and safe designer probiotics. "

This book is devoted to nanomicrobiology and the nanosystems of bacteria. The initial chapter discusses some of the controversies in the geochemical and biomedical fields associated with the reports of nanobacteria in the environment. Current knowledge of several internal and surface structures of bacteria is addressed in this book. Included are chapters discussing carboxysomes, S-layers, gliding motility of bacteria, and aggregation of iron to produce nano-magnetite. Information about the activities of outer membrane vesicles produced by Gram-negative bacteria is discussed as a benefit to bacteria that produce it and some potential industrial applications are presented. A broad review of bacterial-mineral interactions is addressed in a chapter of metallic nanoparticles and colloids production by bacterial reduction of soluble redox active elements. The structures of bacterial nanowires are discussed and their application in extra-cellular electron transport is reviewed. Nanomotor activities of bacteria are discussed as pertains to the mechanics of flagellar rotation, production of energy by ATP synthase, DNA packing, and translocation of proteins across membranes by secretion systems. The rapidly evolving field of nanosystem technology is embracing many areas, and it is the hope that this book will stimulate the use of bacterial nanostructures for future developments in nanotechnology.

Carotenoids are a family of yellow to orange-red terpenoid pigments synthesized by photosynthetic organisms and many bacteria and fungi. They have beneficial health effects protecting against oxidative damage and may be responsible for the colours associated with plants and animals. In "Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols, "expert researchers in the field detail many of the most up-to-date methods which are now commonly used to study carotenoids. These include methods for the study of canthaxanthin production, construction of carotenoid reporter systems, directed evolution of carotenoid synthases, and improvement of b-carotene hydroxylase catalytic activity are described. Additionally, the book includes methods of DNA fingerprinting for the identification of carotenogenic "Dunaliella" species, ketocarotenoid biosynthesis in microalgae expressing the beta-C-4-carotene oxygenase gene, characterization of carotenogenesis genes in "Anabaena" sp., obtaining lutein from microalgal biomass, NMR-based isotopologue profiling of microbial carotenoids, and analysis of diapocarotenoids. Written in the highly successful "Methods in Molecular Biology " series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, " Microbial Carotenoids from Bacteria and Microalgae: Methods and Protocols "provides practical experimental laboratory procedures for a wide range of carotenoids producing microorganisms."

Foodborne illnesses caused by various bacterial, viral, and fungal pathogens lead to a high number of morbidity and mortality in the U.S. and throughout the world. Recent advances in microbial genomics have significantly improved our understanding of the physiology, evolution, ecology, epidemiology, and pathogenesis of different foodborne pathogens. This book focuses on the genomics of foodborne bacterial pathogens. It begins with a brief overview of the recent advances in microbial genomics and the impact of genomics on food safety research. Then, eight chapters follow that elaborate some in-depth reviews on the genomics of several common foodborne bacterial pathogens including Bacillus, Campylobacter, Clostridium, Escherichia coli, Listeria, Salmonella, Staphylococcus, and Vibrio. Finally, the last four chapters focus on some current genomic, transcriptomic, and proteomic technologies and their applications in studying the epidemiology, evolution, and pathogenesis of foodborne bacterial pathogens. Genomics of Foodborne Bacterial Pathogens can be used as a reference by scientists and professionals in academia, government, and industry who are interested in understanding microbial genomics and using genomics tools to study foodborne bacterial pathogens. This book can also be used as a textbook for instructors and professors who teach food microbiology or microbial genomics-related courses at the post-graduate level.

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Systems biology is changing the way biological systems are studied by allowing us to examine the cell and organism as a whole. Systems biotechnology allows optimal design and development of upstream to downstream bioprocesses by taking a systems-approach. E. coli has been a model organism for almost all biological and biotechnological studies. This book brings together for the first time the state-of-the-art reviews by the world-leading experts on systems biology and biotechnological applications of E. coli. The topics covered include genomics and functional genomics, resources for systems biology, network analysis, genome-scale metabolic reconstruction, modelling and simulation, dynamic modelling and simulation, systems-level analysis of evolution, plasmids and expression systems, protein synthesis, production and export, engineering the central metabolism, synthetic biology, and systems metabolic engineering of E. coli. This book provides readers with guidance on how a complex biological system can be studied using E. coli as a model organism. It also presents how to perform synthetic biology and systems metabolic engineering studies on E. coli with successful examples, the approaches of which can be extended to other organisms. This book will be a complete resource for anyone interested in systems biology and biotechnology.

This book will contain a series of solicited chapters that concern with the molecular machines required by viruses to perform various essential functions of virus life cycle. The first three chapters (Introduction, Molecular Machines and Virus Architecture) introduce the reader to the best known molecular machines and to the structure of viruses. The remainder of the book will examine in detail various stages of the viral life cycle. Beginning with the viral entry into a host cell, the book takes the reader through replication of the genome, synthesis and assembly of viral structural components, genome packaging and maturation into an infectious virion. Each chapter will describe the components of the respective machine in molecular or atomic detail, genetic and biochemical analyses, and mechanism. Topics are carefully selected so that the reader is exposed to systems where there is a substantial infusion of new knowledge in recent years, which greatly elevated the fundamental mechanistic understanding of the respective molecular machine. The authors will be encouraged to simplify the detailed knowledge to basic concepts, include provocative new ideas, as well as design colorful graphics, thus making the cutting-edge information accessible to broad audience.

Viruses and Climate Change, Volume 114 in the Advances in Virus Research series, highlights new advances in the field, with this new volume presenting interesting chapters on carbon-cycle and vector-borne viruses. Chapters in this release cover Viruses in the carbon cycle and the impacts on climate change and Climate change and mosquito-borne virus transmission.

Palms are monocots, Angiosperms, belonging to the family Palmae (Arecaceae), perennials having woody stems. Palmae (Arecaceae) family comprised of about six subfamilies, 200 genera and 2,700 species that are distributed all over the tropical, subtropical and Mediterranean landscape. Palms are diverse (ecologically and morphologically) group of plants. Ornamental palms are important component of landscape as well as interiorscapes. Additionally, these plants are good source of food, feed and shelter with numerous other commercial benefits. Likewise other trees and crops, landscape and field nurseries of palms are also subjected to various threats of insect pest and diseases (caused by different plant pathogens). Amongst fungal diseases leaf spots, leaf blights, Fusarium wilts, butt rots, bud rots, root rots, lethal yellowing and decline of palms are major growth constraints of palm growth. In developing countries very little attention has been paid on the etiology and management of these fungal diseases on ornamental palms. Accurate diagnosis and reliable management plan of palm fungal diseases usually requires expertise in both modern and advanced plant pathological approaches. Historically it was general belief that plant pathogens are not associated with human diseases. Since 19th century, several clinical reports are available indicating many plant pathogenic fungi (Aspergillus spp., Penicillium spp., Alternaria spp., Trichoderma spp., Fusarium spp., Curvularia spp. and Colletotrichum Spp) as novel agents of human diseases. Besides the association of fungal plant pathogens infecting ornamental palms, harbouring any of earlier mentioned or other fungal species (capable of causing certain diseases in human beings or pets) by the ornamental palms cultivation (either grown indoor or outdoor) is an important area of research to be explored and addressed thoroughly. This book will provide the deep information regarding major fungal diseases of ornamental palms, their symptoms, disease identification, and etiology and management strategies. This book will also provide unique knowledge regarding the ornamental palms harbouring kinds of human fungal pathogens and their practical management at domestic and commercial scale, in order to make cultivation of these plant more beneficial for humans, animals and environment.

This book examines aspects of paediatric infectious diseases written by leading authorities in the field. It is based on a lecture given at the seventh Infection and Immunity in Children (IIC) course held at the end of June 2009 at Keble College, Oxford.

This is the first English-language book dedicated to Brazilian sand flies and their medical importance. No other country has so many species of these haematophagous insects as Brazil and their diversity has reached an astonishing level. The book contains comprehensive chapters, written by Brazilian experts on their regional distribution, their ecology and their importance as vectors of pathogens and parasites. Methods for sampling, processing and preserving phlebotomines are reviewed as are perspectives on surveillance and leishmaniasis vector control. A novel classification is presented whose aim is to help investigators identify the species that they are working with more efficiently.

Nature helps... of course at first itself by developing measures that give bacteria, fungi, plants and animals a chance to be successful in their struggle for life. As a latecomer on Earth, Homo sapiens was gifted with some droplets of the divine spirit of recognition and thus became able to observe, to analyse and recombine skills of other living beings and to use them for his overwhelming career over the last 10,000 years.Of course fungi, plants, animals and even bacteria were primarily used by mankind as food or as lifestyle products such as beer, but soon it became clear that there was much more potential hidden in these organisms and that they could be used for other purposes, too. Extracts of plants and fungi were recognized as powerful remedies, as medicines, as insecticides or acarizides, as repellents against parasites or even as weapons, e.g. when poisonous compounds from frogs or plants were applied to arrowheads. Over the last 110 years the pharmaceutical industry has often simulated nature by analyzing complex organic substances taken from living organisms and then producing by synthesis absolutely pure compounds, which mostly consisted of only one single active substance. These products had the advantage of acting against precisely one target and thus produced fewer possible side effects than the complex plant extracts. However, the more serious side effect was that disease agents could develop resistances to pure medicinal products much more easily. Thus after 70 years of excellent prospects for chemotherapy, some dark clouds appeared and quickly gathered, so that several therapeutic remedies now no longer work. Therefore in many countries - especially in those where the pure chemotherapeutics are too expensive for the poor population - the cry "back to nature" is becoming louder and louder. This has led to an enormous increase of studies that again use natural extracts as remedies in the fight against diseases. The present book summarizes examples of promising aspects in a broad spectrum of applications and shows how extracts derived from bacteria, marine organisms, plants or even animals may help to treat infectious diseases, how such organisms may keep away parasites and pests from the bodies of plants or animals, including humans, and how they can be used directly to aid in diagnosis, promote wound healing and even to help catch criminals. These 15 chapters offer not only basic research on these different fields, but also show how useful and effective products can be developed from research.

This edited book details the plant-assisted remediation methods, which involves the interaction of plant roots with associated rhizospheric microorganisms for the remediation of soil and water contaminated with high levels of heavy metals, pesticides, radionuclides, agricultural by-products, municipal wastes, industrial solvents, petroleum hydrocarbons, organic compounds, and various other contaminants. Each chapter highlights and compares the beneficial and economical alternatives of phytoremediation to currently practiced soil, water, and air removal. This book covers state-of-the-art approaches in phytoremediation contributed by leading and eminent scientists from across the world. Phytoremediation approaches for environmental sustainability dealing the readers with a cutting-edge of multidisciplinary understanding in the principal and practical approaches of phytoremediation from laboratory research to field application. This book is of interest to researchers, teachers, environmental scientists, environmental engineers, environmentalists, and policy makers. Also, the book serves as additional reading material for undergraduate and graduate students of environmental microbiology, biotechnology, eco-toxicology, environmental remediation, waste management, and environmental sciences as well as the general audience.

Due to the possibility that petroleum supplies will be exhausted in the next decades to come, more and more attention has been paid to the production of bacterial pl- tics including polyhydroxyalkanoates (PHA), polylactic acid (PLA), poly(butylene succinate) (PBS), biopolyethylene (PE), poly(trimethylene terephthalate) (PTT), and poly(p-phenylene) (PPP). These are well-studied polymers containing at least one monomer synthesized via bacterial transformation. Among them, PHA, PLA and PBS are well known for their biodegradability, whereas PE, PTT and PPP are probably less biodegradable or are less studied in terms of their biodegradability. Over the past years, their properties and appli- tions have been studied in detail and products have been developed. Physical and chemical modifications to reduce their cost or to improve their properties have been conducted. PHA is the only biopolyester family completely synthesized by biological means. They have been investigated by microbiologists, molecular biologists, b- chemists, chemical engineers, chemists, polymer experts, and medical researchers for many years. PHA applications as bioplastics, fine chemicals, implant biomate- als, medicines, and biofuels have been developed. Companies have been est- lished for or involved in PHA related R&D as well as large scale production. It has become clear that PHA and its related technologies form an industrial value chain in fermentation, materials, feeds, and energy to medical fields.

This book will cover both the evidence for biofilms in many chronic bacterial infections as well as the problems facing these infections such as diagnostics and treatment regimes. A still increasing interest and emphasis on the sessile bacterial lifestyle biofilms has been seen since it was realized that that less than 0.1% of the total microbial biomass lives in the planktonic mode of growth. The term was coined in 1978 by Costerton et al. who defined the term biofilm for the first time.In 1993 the American Society for Microbiology (ASM) recognised that the biofilmmode of growth was relevant to microbiology. Lately many articles have been published on the clinical implications of bacterial biofilms. Both original articles and reviews concerning the biofilm problem are available.

This volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Schizosaccharomyces pombe: Methods and Protocols hopes to serve as an excellent resource for faculty researchers, undergraduate student researchers, graduate researchers, government, and the medical community.

The discovery that most of the chronic infections in humans, including the oral, lung, vaginal and foreign body-associated infections, are biofilm-based, has prompted the need to design new and properly focused preventive and therapeutic strategies for these diseases. Microbial Biofilms: Methods and Protocols provides a detailed description of the currently available methods and protocols to investigate bacterial and fungal biofilms, exhaustively illustrated and critically annotated in 25 chapters written by authors well known for their experience in the respective fields. The book has joined together microbiologists and specialists in infectious diseases, hygiene and public health involved in exploring different aspects of microbial biofilms as well as in designing new methods and/or developing innovative laboratory protocols. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Microbial Biofilms: Methods and Protocols presents readers with the most established and validated experimental procedures to investigate microbial biofilms.

The book discusses ways to overcome the side effects of using hydrocarbon-based products as energy sources. Hydrocarbons produce raw crude oil waste of around 600,000 metric tons per annum, with a range of uncertainty of 200,000 metric tons per year. The various chapters in this book focus on approaches to reduce these wastes through the application of potential microbes, in a process called bioremediation. The book is a one-stop reference resource on the methods, mechanisms and application of the bio-composites, in the laboratory and field. Focusing on resolving a very pressing environmental issue, it not only provides details of existing challenges, but also offers deeper insights into the possibility of solving problems using hydrocarbon bioremediation.

The book summarises the current understanding of the Nervous -, Endocrine and Immune systems with emphasis on shared mediators and receptors and functional interaction. In addition to the fundamental physiological and pathophysiological mechanisms, which are presented in detail, some clinically relevant subjects are also presented, such as inflammation, asthma and allergy, autoimmune disease, immunodeficiency and the acute phase response.

- A comprehensive presentation of neuroimmune biology
- Introduces the subject matter to the uninformed reader
- Contains basic information, theoretical considerations and up-to-date clinical chapters
- The clinical chapters will be helpful to practising physicians

This book provides the first comprehensive review of Acanthamoeba research. Everything that is known about Acanthamoeba is critically reviewed and divided into easy-to-follow sections. The book presents the current state of research on every aspect of this organism, detailing major advances in areas such as genomics, molecular and cellular biology, life cycles, geographical distribution, role in ecosystem, morphology, motility, phylogenetics, genotyping, metabolism, regulation of morphogenesis, host-parasite interactions, the molecular and immunological basis of pathogenesis, methods of transmission, epidemiology, clinical manifestation, diagnosis, treatment, new target development and drug resistance, as well as its role as a Trojan horse of the microbial world, including viral, bacterial, protozoal and fungal pathogens, and much more. There is a significant emphasis on the knowledge of Acanthamoeba infections that has grown in the molecular era. In addition, this book provides a historical perspective on Acanthamoeba research that will be of considerable interest. This compilation will serve as an essential reference for microbiologists, immunologists, and physicians in the field of basic and medical microbiology, as well as an invaluable reference for new and experienced researchers who wish to understand this organism better. The book is the definitive guide to current research on this increasingly important organism.