Books > Science & Mathematics > Physics > Applied physics & special topics > Biophysics
|
Buy Now
Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition (Paperback, Softcover reprint of the original 1st ed. 2017)
Loot Price: R2,789
Discovery Miles 27 890
|
|
Dynamics and Mechanism of DNA-Bending Proteins in Binding Site Recognition (Paperback, Softcover reprint of the original 1st ed. 2017)
Series: Springer Theses
Expected to ship within 10 - 15 working days
|
Using a novel approach that combines high temporal resolution of
the laser T-jump technique with unique sets of fluorescent probes,
this study unveils previously unresolved DNA dynamics during search
and recognition by an architectural DNA bending protein and two DNA
damage recognition proteins. Many cellular processes involve
special proteins that bind to specific DNA sites with high
affinity. How these proteins recognize their sites while rapidly
searching amidst ~3 billion nonspecific sites in genomic DNA
remains an outstanding puzzle. Structural studies show that
proteins severely deform DNA at specific sites and indicate that
DNA deformability is a key factor in site-specific recognition.
However, the dynamics of DNA deformations have been difficult to
capture, thus obscuring our understanding of recognition
mechanisms. The experiments presented in this thesis uncover, for
the first time, rapid (~100-500 microseconds) DNA unwinding/bending
attributed to nonspecific interrogation, prior to slower (~5-50
milliseconds) DNA kinking/bending/nucleotide-flipping during
recognition. These results help illuminate how a searching protein
interrogates DNA deformability and eventually "stumbles" upon its
target site. Submillisecond interrogation may promote preferential
stalling of the rapidly scanning protein at cognate sites, thus
enabling site-recognition. Such multi-step
search-interrogation-recognition processes through dynamic
conformational changes may well be common to the recognition
mechanisms for diverse DNA-binding proteins.
General
Is the information for this product incomplete, wrong or inappropriate?
Let us know about it.
Does this product have an incorrect or missing image?
Send us a new image.
Is this product missing categories?
Add more categories.
Review This Product
No reviews yet - be the first to create one!
|
|
Email address subscribed successfully.
A activation email has been sent to you.
Please click the link in that email to activate your subscription.