Cotton is the most important textile and cash crop and is widely cultivated in more than 70 countries, including the United States, China, and India. Because its long life cycle and complicated genetic background, it is hard to improve cotton using traditional breeding techniques although it has made much progress in the last several decades. Currently, transgenic techniques have become a powerful tool to improve cotton and transgenic cotton is among the first commercially genetically modified crops. "Transgenic Cotton: Methods and Protocols" provides a comprehensive collection of methods for creating and monitoring transgenic cotton and its application on agricultural and basic research. Divided into five convenient sections, topics covered include the current status and perspectives of transgenic cotton, the principle and methods for making transgenic cotton, the methods for detecting foreign gene copy and expression in transgenic plants, the improvement of cotton using transgenic technology, and finally the methods for monitoring the potential impact of transgenic cotton on environment, including gene flow. Written in the successful "Methods in Molecular Biology " series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, "Transgenic Cotton: Methods and Protocols" will serve as an excellent resource for scientists as well as graduate students who work on transgenic plants, plant genetics, molecular biology and agricultural sciences. "

This book covers the latest developments in tissue electrical conductivity and current density imaging, increasingly popular as well as challenging applications of MRI. These applications are enabled by the acquisition of high-quality MR phase images. This book provides a practical description of the MRI physics needed to understand and acquire phase images in MRI and the key details required to reconstruct them into conductivity, current density or electric field distributions. Comprehensive details are provided about the electrical properties of biological tissues, computational modeling considerations, experimental methods, construction of non-biological and biological phantoms and MRI pulse sequences. An inclusive review of image reconstruction algorithms, and their potential applications is provided for applications directed at determining current density or electric fields, such as in transcranial DC or AC stimulation techniques; as well as electrical conductivity reconstructions that may be of use in quantitative MRI applications used to detect cancer or other pathologies. This is an excellent book for undergraduate and graduate students beginning to explore phase, current density, and conductivity imaging in MRI, and will also be of great use to researchers interested in the area of MR-based electrical property imaging.

This book presents deliberations on molecular and genomic mechanisms underlying the interactions of crop plants to the abiotic stresses caused by heat, cold, drought, flooding, submergence, salinity, acidity, etc., important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomic-assisted breeding, and the recently emerging genome editing for developing resistant varieties in fruit crops is imperative for addressing FHNEE (food, health, nutrition, energy, and environment) security. Whole genome sequencing in many of these crops followed by genotyping-by-sequencing has provided precise information regarding the genes conferring resistance useful for gene discovery, allele mining, and shuttle breeding which in turn opened up the scope for 'designing' crop genomes with resistance to abiotic stresses. The seven chapters each dedicated to a fruit crop and a fruit crop group in this volume elucidate different types of abiotic stresses and their effects on and interaction with the crops; enumerate the available genetic diversity with regard to abiotic stress resistance among available cultivars; illuminate the potential gene pools for utilization in interspecific gene transfer; present brief on classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; depict the success stories of genetic engineering for developing abiotic stress-resistant crop varieties; discuss on molecular mapping of genes and QTLs underlying stress resistance and their marker-assisted introgression into elite varieties; enunciate different genomics-aided techniques including genomic selection, allele mining, gene discovery, and gene pyramiding for developing adaptive crop varieties with higher quantity and quality of yields, and also elaborate some case studies on genome editing focusing on specific genes for generating abiotic stress-resistant crops.

A reference is needed that addresses the recent progress in aspects of PK/PD methods and developments of nanoparticles for novel drug delivery systems. No other consolidated published reference discusses the PK/PD study of nanoparticle drug delivery systems. This book discusses the advantages of nanoparticle drug delivery systems (NPDDS) in enhancing the pharmacokinetics of many drugs that are not easily metabolized or that obtain the desired therapeutic effect with minimum toxicity. The authors provide an overview of biodistribution with a focus on polymer and lipid nanoparticles. This thorough reference is divided into three parts: Modelling, Specific carries and their potential to treat specific diseases.

This book is an inclusive coverage of advances in aquaculture health management. It offers latest updates as well as explains the novel concepts and issues related to aquatic animal health management. To support the understanding of the concepts, there is extensive use of illustrations. Chapters emphasize on the state of art techniques and hold great promise for the sustainable development of aquaculture. This book is of interest to teachers, researchers, aquatic biologists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of aquatic sciences, marine sciences, biotechnology, ecology, and environmental sciences. National and international aquatic scientists, policy makers will also find this to be a useful read.

This edited book provides the first comprehensive overview on conventional and emerging processing technologies for the extraction and purification of proteins and/or peptides from plant sources with a special focus on subsequent product development. The book opens with an introduction to the most conventional processing technologies used in industry today: the alkaline extraction followed by isoelectric precipitation, and air classification. The book also focusses on novel extraction and purification technologies, covering the most recent green emerging technologies based on enzymatic processes, solvents, high-pressure processing, barometric membrane technologies, and microwave-assisted extraction, among others. The final chapters bridge the gap between the presented methods and product development and highlight how these technologies can alter protein functionality and nutritional quality of the extracted protein, and thereby, impact human health. In the context of rising consumer interest in foods from plant-protein ingredients and the United Nations targets for Sustainable Development Goal 12 on 'Responsible Consumption and Production', this book will provide an indispensable resource for students, engineers and researchers in academia and industry, working in the area of food science, food technology and plant-based product development.

This contributed volume presents the current state of research on regenerative rehabilitation across a broad range of neuro- and musculoskeletal tissues. At its core, the primary goal of regenerative rehabilitation is to restore function after damage to bones, skeletal muscles, cartilage, ligaments/tendons, or tissues of the central and peripheral nervous systems. The authors describe the physiology of these neuro- and musculoskeletal tissue types and their inherent plasticity. The latter quality is what enables these tissues to adapt to mechanical and/or chemical cues to improve functional capacity. As a result, readers will learn how regenerative rehabilitation exploits that quality, to trigger positive changes in tissue function. Combining basic, translational, and clinical aspects of the topic, the book offers a valuable resource for both scientists and clinicians in the regenerative rehabilitation field.

Genome Mapping and Molecular Breeding in Plants presents the current status of the elucidation and improvement of plant genomes of economic interest. The focus is on genetic and physical mapping, positioning, cloning, monitoring of desirable genes by molecular breeding and the most recent advances in genomics. The series comprises seven volumes: Cereals and Millets; Oilseeds; Pulses, Sugar and Tuber Crops; Fruits and Nuts; Vegetables; Technical Crops; and Forest Trees.

Pulses, Sugar and Tuber Crops includes reviews in 15 chapters contributed by 47 eminent scientists from 10 countries. The chapters on common bean, pea, cowpea, sugarcane and potato include comprehensive reviews of voluminous research findings. Fundamental aspects and molecular results are presented for eight orphan crops of high agroeconomic importance, including mungbean, lentil, chickpea, lathyrus, pigeonpea, sweet potato, cassava and yam. Additionally, works on quinoa and Bambara groundnut are reviewed for the first time."

This Volume describes methods for simulating natural environments by using reproducible and controllable meso- and microcosm experiments to analyse hydrocarbon-degrading microorganisms and to test hypotheses. It presents important aspects of the preparation of experimental set-ups, hydrocarbon application and sampling, and features protocols for experiments with different types of samples, such as biofilms, aquatic systems (e.g. groundwater, streams), and sediments, including oscillating oxic-anoxic mesocosms, as well as for in-situ experimentation in subtidal and deep sediments. Two chapters are dedicated to cultivation under high-pressure conditions, and several chapters include protocols for processing samples for downstream chemical, microbial or activity analyses. Several of the approaches presented are generic and will benefit anyone embarking on designing meso- and microcosm experiments. < Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

This detailed volume explores perspectives and methods using cell-free expression (CFE) to enable next-generation synthetic biology applications. The first section focuses on tools for CFE systems, including a primer on DNA handling and reproducibility, as well as methods for cell extract preparation from diverse organisms and enabling high-throughput cell-free experimentation. The second section provides an array of applications for CFE systems, such as metabolic engineering, membrane-based and encapsulated CFE, cell-free sensing and detection, and educational kits. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cell-Free Gene Expression: Methods and Protocols serves as an ideal guide for researchers seeking technical methods to current aspects of CFE and related applications.

This book focuses on a broad range of immunomodulators, including both natural and synthetic ones. It highlights their role in human health. The book discusses nanotechnology-based immunomodulators with special emphasis on different types of formulation to boost the immune system. It highlights the role of immunomodulators against different microbes. This book describes important topics such as Classification and uses of various immunomodulators; Potential role of herbs and spices on immune system; Immune booster activity of prebiotics; Recent advances of nutraceutical and functional foods in Immune health; Antioxidants and immunomodulation; Nanotechnology and immunomodulators in cancer; Immunomodulatory role for the treatment and management of tuberculosis; Immunomodulation in autoimmune disorders; Herbal immunomodulators and COVID-19; Role of Cytokines as Immunomodulators and Immunomodulatory effects of endocrine disrupting chemicals. This book covers various aspects of product innovation, development, and launching of nutritional and pharmaceutical products with reference to immunomodulators. This book is meant for researchers and students in the field of immunology and pharmaceutical sciences.

The edited book covers all potential products from microalgal-based biorefinery having the focus on contemporary technologies and future outlook. Along with the focus on microalgal biorefinery products, the book also focuses on biotechnological advances via the utilization of modern molecular biology, system biology, synthetic biology, or metabolic engineering approach in microalgal biorefinery. The development of any technologies has a direct effect on the human being and the environment, therefore, the socio-economic, techno-economic, and environmental impact of the microalgae-based biorefineries will also be included in the book. In microalgal biomass-based biorefinery different biofuel- biodiesel, bioethanol, bio-hydrogen, and value-added compounds such as carotenoids, fatty acids, and protein can be produced simultaneously. Understanding the technical advances to develop an integrated biorefinery approach with the motive of designing a consolidated self-sustainable microalga-based biorefinery. This book is equally beneficial for researchers and engineers in biomass-based biorefineries or the bachelors, master, or young budding graduate students as a textbook.

This edited book is a comprehensive compilation highlighting sources of biostimulants, their production, influence on plant growth and development, and regulatory status of plant biostimulants for better understanding and opening new vistas for future research. Biostimulants, the biological formulations are known to meliorate the plants growth and vigour, improve nutritional efficiency along with maintaining their well-being mainly via providing protection against a wide range of infections. Both horticultural as well as agricultural crops involve the utilization of the biostimulants. Fulvic and humic acids, nitrogen-containing compounds, protein hydrolysates, favourable bacteria and fungi, and extracts of seaweed are the chief active components of these. The major driving force for these materials is the organic farming industry and demand for sustainable crop production. This book will be of great interest to researchers, teachers, climate change scientists, capacity builders, and policy makers. Moreover, this book does the work of a supplementary reading for students in various fields such as agriculture, soil science, ecology, environmental science and forestry at undergraduate as well as graduate level. This will be a gainful read for national and international agricultural scientists and the policy makers. * Elaborates on biostimulants induced influence of plant growth and development * Covers all aspects of biostimulants sources and its role in plant life in detail * Discusses evidence based approach in biostimulants sources and its useful applications in plants

This self contained book presents a comprehensive overview of the past, present and future of the galactose regulon of yeast, the classical model system of molecular biologists. The book starts with a brief historical overview on yeast research. This is followed by molecular genetics of the galactose regulon, isolation of genes and testing of the hypotheses. Contemporary topics including genomics, evolution, binary and graded responses, and stochasticity are all addressed.

DNA Vaccines: Methods and Protocols, Third Edition explores innovative approaches and technologies used to design, deliver, and enhance the efficacy of DNA vaccines. Featuring applications which should be of great value in moving vaccines from research to clinic, this detailed volume includes sections on DNA vaccine design and enhancement, delivery systems, production, purification, and quality, as well as chapters on new vaccine applications. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Vaccines: Methods and Protocols, Third Edition serves the important role of further documenting the potential of the DNA vaccination as a platform technology for treatment and prevention of human disease.

A leading artificial intelligence researcher lays out a new approach to AI that will enable us to coexist successfully with increasingly intelligent machines In the popular imagination, superhuman artificial intelligence is an approaching tidal wave that threatens not just jobs and human relationships, but civilization itself. Conflict between humans and machines is seen as inevitable and its outcome all too predictable. In this groundbreaking book, distinguished AI researcher Stuart Russell argues that this scenario can be avoided, but only if we rethink AI from the ground up. Russell begins by exploring the idea of intelligence in humans and in machines. He describes the near-term benefits we can expect, from intelligent personal assistants to vastly accelerated scientific research, and outlines the AI breakthroughs that still have to happen before we reach superhuman AI. He also spells out the ways humans are already finding to misuse AI, from lethal autonomous weapons to viral sabotage. If the predicted breakthroughs occur and superhuman AI emerges, we will have created entities far more powerful than ourselves. How can we ensure they never, ever, have power over us? Russell suggests that we can rebuild AI on a new foundation, according to which machines are designed to be inherently uncertain about the human preferences they are required to satisfy. Such machines would be humble, altruistic, and committed to pursue our objectives, not theirs. This new foundation would allow us to create machines that are provably deferential and provably beneficial.

This updated and expanded second edition reviews numerous aspects of the marine microbiome and its possible industrial applications. The marine microbiome is the total of microorganisms and viruses in the ocean and seas and in any connected environment, including the seafloor and marine animals and plants. In the first part of the book, diversity, origin and evolution of the marine microorganisms and viruses are discussed. The microbes presented originate from all three domains of life: Bacteria, Archaea, and Eukarya. The second part sheds some light on the different communities: it describes marine habitats and how their inhabitants control biogeochemical cycles. The third part finally examines the microbial ocean as a global system and evaluates methods of utilizing marine microbial resources. Adopting a translational approach, the book connects academic research with industrial applications, making it a fascinating read and valuable resource for microbiologists from both domains.

Advances in Applied Microbiology, Volume 120 continues the comprehensive reach of this widely read and authoritative review source in microbiology, providing invaluable references and information on a variety of areas relating to the topics of microbiology.

The Flax Genome is a comprehensive compilation of most recent studies focused on reference genome, genetic resources and molecular diversity, breeding, QTL mapping, gene editing tools, functional genomics and metabolomics, molecular breeding via genomic selection, and genomic resources. The flax genome reference sequences and the new genome assemblies are presented. A list of flax QTL and candidate genes associated with more than 35 traits, including yield and agronomic, seed quality and fatty acid composition, fibre quality and yield, abiotic stress, and disease resistance traits, are summarized. A QTL- based genomic selection strategy and genome-editing tools are systematically introduced. In addition, huge amounts of flax genomic resources generated in the last decade are summarized. The book contains 13 chapters with about 390 pages authored by globally reputed researchers in the relevant fields to this crop The book is intended to be useful to students, teachers, and researchers interested in traditional and molecular breeding, pathology, molecular genetics and breeding, bioinformatics and computational biology, and functional genomics

Animal cell technology has undergone a rapid transformation over the last decade from a research tool and highly specialised technology to a central resource for innovation in pharmaceutical research and development. These proceedings of the 14th Meeting of the European Society for Animal Cell Technology (Vilamoura, Portugal, May 1996) bring up to date the historical perspective of animal cell technology for the benefit of society, `From Vaccines to Genetic Medicine', and will charter this vital technology for the years to come. Strong contributions are grouped in the traditional ESACT areas of 'Cell and Physiology Engineering' dealing with cell state, including genetics, and its environment, and 'Animal Cell Process Engineering' covering integration of bioreaction with bioseparation coupled with on-line monitoring to improve protein production and consistency. Extensive coverage of metabolic engineering on synthesis, folding, assembly, transiting and secretion is dealt with in the session on 'Recombinant Proteins: Biosynthesis and Bioprocessing'. Two traditional but expanding areas of animal cell technology relevance are highlighted in the broad sessions of 'Animal Cells as Tools for Discovery and Testing' and 'Animal Cell Vaccines: Present and Future'. Two sessions finally cover the more recent domains of animal cell technology work - 'Tissue Engineering and Biomedical Devices' and 'Cells and Vectors for Genetic Medicine' - where one can foresee a very bright future.

This book discusses the various aspects of sustainable packaging edibles in food industry. It is divided into five main parts. The first section of the book addresses details of edible films, various sources, origin, scope and functions. Second section covers different sustainable alternatives such as seed gums, fruits and vegetable peels, sea weeds, fruits wastes, dairy by products & anti-oxidant edible packaging. This book also discusses about methods of improvements of mechanical properties of packaging edibles & their food applications, testing methods, innovations, limitations, challenges and nano edibles. It provides insights about the large quantity of wastes and by-products generated by food processing industries. Disposal of these wastes is a big problem due to their high biochemical oxygen demand (BOD) & chemical oxygen demand (COD) which causes severe problem of pollution to the environment. These wastes contain large amounts of proteins, carbohydrates, lipids, minerals, various bioactive compounds and have eco-friendly packaging potential. The book emphasizes on the fact that recycling these wastes as packaging edibles are sustainable and economical. As a world foreseeing food technology revolution, this book explores the unique topics in food packaging which possesses mammoth commercial applications and environmental potential. Due to its immense scope, this book is highly useful for researchers, food scientists, students and food packaging industry experts.

This book is the ultimate assembly of recent research activities on molecular architectonics and nanoarchitectonics by authors who are worldwide experts. The book proposes new ways of creating functional materials at the nano level using the concepts of molecular architectonics and nanoarchitectonics, which are expected to be the next-generation approaches beyond conventional nanotechnology. All the contents are categorized by types of materials, organic materials, biomaterials, and nanomaterials. For that reason, non-specialists including graduate and undergraduate students can start reading the book from any points they would like. Cutting-edge trends in nanotechnology and material sciences are easily visible in the contents of the book, which is highly useful for both students and experimental materials scientists.

The aim of the Technical Advisory Committee, in planning the c~:>Dtent of this meeting, was to illustrate the range of separation processes in which the use of membranes was practical and effective at an industrial scale. As Professor Strathmann reveals, the market for process equipment built around membranes is now worth about $5x1(f annually, and it seemed important to review this technology, and to point the direction of future technical advances. All but the most critical reader should find some items of interest. The Committee would admit to not fulftlling all of thier aims, although those delegates who attended the meeting in Edinburgh judged it a success. In the event it provided representative examples of processes from the food and beverage industry, from water treatment, and from the chemical industry, of which the removal of alcohol from fermented beverages, shipboard desalination and solvent recovery are three. The major uses of charged membranes and sterile processes are not covered, nor 9 is the largest market, $1.2x10 annually, for artificial kidney dialysis. However, it is interesting to see artificial kidney now finding an alternative use as a reactor for the production of monoclonal antibodies. We are also reminded by Professor Michel of the importance and efficiency of natural membranes in the kidney under conditions where fouling is crucial to their performance and enhances their selectivity.

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science.Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification.In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds.

The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.