Genome sequence studies have become more and more important for plant breeding. Brassicas and Legumes: From Genome Structure to Breeding comprises 16 chapters and presents both an overview and the latest results of this rapidly expanding field. Topics covered include: genome analysis of a flowering plant, Arabidopsis thaliana; the sequence of the Arabidopsis genome as a tool for comparative structural genomics in Brassicaceae; application of molecular markers in Brassica coenospecies; the molecular genetic basis of flowering time variation in Brassica species; quantitative trait loci for clubroot resistance in Brassica oleracea; structural differences of S locus between Brassica oleracea and Brassica rapa; Brassica and legume chromosomes; sequence analysis of the Lotus japonicus genome; introduction of an early flowering accession ‘Miyakojima’ MG-20 to molecular genetics in Lotus japonicus; genetic linkage map of the model legume Lotus japonicus; construction of a high quality genome library of Lotus japonicus; genome analysis of Mesorhizobium loti: a symbiotic partner to Lotus japonicus; molecular linkage map of the model legume Medicago truncatula; genetic mapping of seed and nodule protein markers in diploid alfalfa (Medicago sativa); mapping the chickpea (Cicer arietinum) genome: localization of fungal resistance genes in interspecific crosses.  

Biotechnology Biotechnology is is now now established established as as a a major major area area of of technology, technology, concerned concerned with with the' the' application application of of biological biological organisms, organisms, systems systems or or processes processes to to manufac turing turing or or service service industries'. industries'. Although Although the the exploitation exploitation of of organisms organisms by by man man is is not not new, new, many many of of the the techniques techniques which which are are stimulating stimulating the the rapid rapid advances advances in in biotechnology biotechnology have have developed developed from from recent recent scientific scientific discoveries. discoveries. Throughout Throughout history history man man has, has, knowingly knowingly or or not, not, been been exploiting exploiting yeast yeast in in the the production production of of alcoholic alcoholic beverages beverages and and bread, bread, and and these these processes processes still still represent represent major major biotechnological biotechnological industries. industries. The The brewer's brewer's and and baker's baker's yeast yeast Sac charomyces charomyces cerevisiae cerevisiae is, is, however, however, also also a a favoured favoured organism organism for for the the production production of of many many new new biotechnological biotechnological products. products."

Dynamic Modeling of Musculoskeletal Motion introduces biomechanists at all levels of expertise to modern methods of modeling and analyzing dynamic biomechanical systems in three dimensions. Using vector kinematics, the reader is taught a systematic method which significantly reduces the complexity of working with multiple, moving limb segments in three dimensions. Operations which usually require the application of differential calculus are replaced by simple algebraic formulae. To derive dynamical equations of motion, a practical introduction to Kane's Method is given. Kane's Method builds upon the foundation of vector kinematics and represents one of the most exciting theoretical developments of the modern era. Together, these techniques enable biomechanists to decipher and model living systems with great realism, efficiency and accuracy. Using these methods, much more time can be spent on biomechanical issues, and much less time must be expended tediously deriving equations of motion. Interwoven with the theoretical presentation are chapters and examples which highlight the subtle differences between inanimate linkages and the biomechanical systems we seek to understand.
About the Cover - Dancer Alea Canning twirls a girl at the Lirio de los Valles orphanage in Baja California, Mexico. Photograph and artwork by Gary T. Yamaguchi.
Solutions Manual: Course instructors may request a copy of solutions to the exercises listed at the end of each chapter by sending a written request to: Gary T. Yamaguchi, Ph.D., Managing Engineer, Biomechanics Practice, ExponentA, A(R) Failure Analysis AssociatesA, A(R), 23445 North 19th Avenue / Phoenix, AZ 85027 or via email to

[email protected] send information regarding the instructor's name, title, and institution, the department, course number and title, the semester and year the course will be taught, and the expected enrollment.

This edited book provides the first comprehensive overview on conventional and emerging processing technologies for the extraction and purification of proteins and/or peptides from plant sources with a special focus on subsequent product development. The book opens with an introduction to the most conventional processing technologies used in industry today: the alkaline extraction followed by isoelectric precipitation, and air classification. The book also focusses on novel extraction and purification technologies, covering the most recent green emerging technologies based on enzymatic processes, solvents, high-pressure processing, barometric membrane technologies, and microwave-assisted extraction, among others. The final chapters bridge the gap between the presented methods and product development and highlight how these technologies can alter protein functionality and nutritional quality of the extracted protein, and thereby, impact human health. In the context of rising consumer interest in foods from plant-protein ingredients and the United Nations targets for Sustainable Development Goal 12 on 'Responsible Consumption and Production', this book will provide an indispensable resource for students, engineers and researchers in academia and industry, working in the area of food science, food technology and plant-based product development.

With contributions from nearly 130 internationally renowned experts in the field, this reference details advances in transgenic plant construction and explores the social, political, and legal aspects of genetic plant manipulation. It provides analyzes of the history, genetics, physiology, and cultivation of over 30 species of transgenic seeds, fruits, and vegetables. Stressing the impact of genetic engineering strategies on the nutritional and functional benefit of foods as well as on consumer health and the global market economy, the book covers methods of gene marking, transferring, and tagging public perceptions to the selective breeding, hybridization, and recombinant DNA manipulation of food.

This book addresses Synthetic Biology (SynBio), a new and promising biotechnology that has attracted much interest from both a scientific and a policy perspective. Yet, questions concerning the patentability of SynBio inventions have not been examined in detail so far; as a result, it remains unclear whether these inventions are patentable on the basis of current norms and case law. The book addresses this question, focusing especially on the subject matter's eligibility and moral criteria. It provides an overview of the legislation and decisions applicable to SynBio patents and examines this new technology in view of the ongoing debate over the patentability of biotechnologies in general. The legal analysis is complemented by the practical examination of several patent applications submitted to the European and US patent offices (EPO and USPTO), and by an assessment of the patent issues that are likely to be raised by future SynBio developments.

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

The Flax Genome is a comprehensive compilation of most recent studies focused on reference genome, genetic resources and molecular diversity, breeding, QTL mapping, gene editing tools, functional genomics and metabolomics, molecular breeding via genomic selection, and genomic resources. The flax genome reference sequences and the new genome assemblies are presented. A list of flax QTL and candidate genes associated with more than 35 traits, including yield and agronomic, seed quality and fatty acid composition, fibre quality and yield, abiotic stress, and disease resistance traits, are summarized. A QTL- based genomic selection strategy and genome-editing tools are systematically introduced. In addition, huge amounts of flax genomic resources generated in the last decade are summarized. The book contains 13 chapters with about 390 pages authored by globally reputed researchers in the relevant fields to this crop The book is intended to be useful to students, teachers, and researchers interested in traditional and molecular breeding, pathology, molecular genetics and breeding, bioinformatics and computational biology, and functional genomics

The biotechnology industry across the globe is growing dramatically in line with rapidly emerging scientific and technological developments. This book explores both the theoretical and practical aspects of entrepreneurship in the biotechnology industry, focusing on the innovation processes underpinning success for new biotechnology firms (NBFs). It argues that biotechnology is at a crossroads: to date the science has been solid, yet commercial success remains elusive, and that it will be the commercial success of NBFs which will dictate the long term viability of this crucial industry. The authors go on to examine the roles played by both entrepreneurship and innovation in the competitiveness of biotechnology companies through a focus on: intellectual property strategies, product development, valuing biotechnology ventures, funding innovation and R&D, alliances and networking, changing industry structures evidenced through the shifting value chain and the impact of globalization on the changing industry and organizational life cycles. International case studies with a focus on human biosciences support the important theoretical developments at the heart of this book. Innovation and Entrepreneurship in Biotechnology offers original and valuable insights to researchers, academics and students as well as to practitioners involved with innovation and entrepreneurship in the field of biotechnology.

The marine environment has been, and continues to be, a fruitful source of novel chemical compounds that are not found in terrestrial and freshwater organisms. Many of these substances show potential biomedical applications, which could lead to development of new pharmaceutical products. Research on the utilization of natural products from marine organisms is growing by leaps and bounds; one important reason why being that, investigators, through new diving technologies, are becoming able to explore at greater depths. Studies of these marine natural products include investigations of neuronal membrane-active toxins, ion channel blockers, antitumor and antiviral agents, and anti-inflammatory molecules. This volume is the sixth in the ongoing series.

Comprehensive Biotechnology, Third Edition, Six Volume Set unifies, in a single source, a huge amount of information in this growing field. The book covers scientific fundamentals, along with engineering considerations and applications in industry, agriculture, medicine, the environment and socio-economics, including the related government regulatory overviews. This new edition builds on the solid basis provided by previous editions, incorporating all recent advances in the field since the second edition was published in 2011.

Global industrial growth has resulted in numerous pollutants being introduced into the environment. It has additionally caused decreased water availability for agricultural activity in developing countries, which, in turn, has compelled farmers to use wastewater irrigation. In advanced agricultural systems, farmers are adapting various strategies to achieve a higher yield and thus sustain crop productivity. Consequent to the introduction of contaminants in the environment, soil pollutants have become a critical issue. Selection of disease-resistant, high-yielding crop varieties, and extensive fertilizer applications are quite common among farming communities. This book provides insight into environmental pollutants with special reference to their interference with plant nutrition. It additionally discusses the physiological aspects of plant nutrition. This book enhances current knowledge of the effects of pollutants on plant growth and physiology.

Biodesign in the Age of Artificial Intelligence: Deep Green investigates the potential of nature based technology for shaping the evolution of contemporary architecture and design. It takes on the now pervasive topic of design intelligence, extending its definition to encompass both biological and digital realms. As in their first title, Systemic Architecture: Operating Manual for the Self Organizing City, the authors engage the topic through the specific lens of their innovative design practice, ecoLogicStudio and their research at the University of Innsbruck and at the Bartlett, UCL. Part One of the book, entitled PhotoSynthetica (TM), illustrates design solutions that engage the urban microbiome and seek to achieve an immediate impact, while Part Two, entitled Deep Green, includes synthetic landscapes and operates within a much larger spatio-temporal frame, going beyond human perception and life span to envision design as a geographical and geological force. In the age of catastrophic climate change, such perceptual expansion helps to clarify that change cannot simply be stopped or rolled back. We must instead establish more positive dynamics of change within the living world. To this end, this book proposes to engage with design and architecture as an extended cognitive interface, a sentient being that is co-evolutionary and symbiotic with the living planet, contributing to its beauty and to our continued enjoyment of it.

Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Nanotechnology has shown great potential in all spheres of life. With the increasing pressure to meet the food demands of rapidly increasing population, thus, novel innovation and research are required in agriculture. The principles of nanotechnology can be implemented to meet the challenges faced by agricultural demands. Major challenges include the loss of nutrients in the soil and nutrient-deficient plants, which result in a lower crop yield and quality. Subsequently, consumption of such crops leads to malnourishment in humans, especially in underprivileged and rural populations. One convenient approach to tackle nutrient deficiency in plants is via the use of fertilizers; however, this method suffers from lower uptake efficiency in plants. Another approach to combat nutrient deficiency in humans is via the use of supplements and diet modifications; however, these approaches are less affordably viable in economically challenged communities and in rural areas. Therefore, the use of nano-fertilizers to combat this problem holds the greatest potential. Additionally, nanotechnology can be used to meet other challenges in agriculture including enhancing crop yield, protection from insect pests and animals, and by use of nano-pesticides and nano-biosensors to carry out the remediation of polluted soils. The future use of nanomaterials in soil ecosystems will be influenced by their capability to interact with soil constituents and the route of nanoparticles into the environment includes both natural and anthropogenic sources. The last decade has provided increasing research on the impact and use of nanoparticles in plants, animals, microbes, and soils, and yet these studies often lacked data involving the impact of nanoparticles on biotic and abiotic stress factors. This book provides significant recent research on the use of nano-fertilizers, which can have a major impact on components of an ecosystem. This work should provide a basis to further study these potential key areas in order to achieve sustainable and safe application of nanoparticles in agriculture.

The book discusses the importance of eggplant (Solanum melongena L.) as a crop, highlighting the potential for eggplant to serve as a model for understanding several evolutionary and taxonomic questions. It also explores the genomic make-up, in particular in comparison to other Solanaceous crops, and examines the parallels between eggplant and tomato domestication as well as between the most common eggplant species and two related eggplants native to Africa (Ethiopian eggplant [Solanum aethiopicum L.] and African eggplant [Solanum macrocarpon L.]). The eggplant genome was first sequenced in 2014, and an improved version was due to be released in 2017. Further investigations have revealed the relationships between wild species, domesticated eggplant, and feral weedy eggplant (derived from the domesticate), as well as targets of selection during domestication. Parallels between eggplant and tomato domestication loci are well known and the molecular basis is currently being investigated. Eggplant is a source of nutrition for millions of people worldwide, especially in Southeast Asia where it is a staple food source. Domesticated in the old world, in contrast to its congeners tomato and potato, the eggplant is morphologically and nutritionally diverse. The spread of wild eggplants from Africa is particularly interesting from a cultural point of view. This book brings together diverse fields of research, from bioinformatics to taxonomy to nutrition to allow readers to fully understand eggplant's importance and potential.

This book covers the latest developments in tissue electrical conductivity and current density imaging, increasingly popular as well as challenging applications of MRI. These applications are enabled by the acquisition of high-quality MR phase images. This book provides a practical description of the MRI physics needed to understand and acquire phase images in MRI and the key details required to reconstruct them into conductivity, current density or electric field distributions. Comprehensive details are provided about the electrical properties of biological tissues, computational modeling considerations, experimental methods, construction of non-biological and biological phantoms and MRI pulse sequences. An inclusive review of image reconstruction algorithms, and their potential applications is provided for applications directed at determining current density or electric fields, such as in transcranial DC or AC stimulation techniques; as well as electrical conductivity reconstructions that may be of use in quantitative MRI applications used to detect cancer or other pathologies. This is an excellent book for undergraduate and graduate students beginning to explore phase, current density, and conductivity imaging in MRI, and will also be of great use to researchers interested in the area of MR-based electrical property imaging.

The consumption of petroleum has surged during the 20th century, at least partially because of the rise of the automobile industry. Today, fossil fuels such as coal, oil, and natural gas provide more than three quarters of the world's energy. Unfortunately, the growing demand for fossil fuel resources comes at a time of diminishing reserves of these nonrenewable resources. The worldwide reserves of oil are sufficient to supply energy and chemicals for only about another 40 years, causing widening concerns about rising oil prices. The use of biomass to produce energy is only one form of renewable energy that can be utilized to reduce the impact of energy production and use on the global environment. Biomass can be converted into three main products such as energy, biofuels and fine chemicals using a number of different processes. Today, it is a great challenge for researchers to find new environmentally benign methodology for biomass conversion, which are industrially profitable as well. This book focuses on the conversion of biomass to biofuels, bioenergy and fine chemicals with the interface of biotechnology, microbiology, chemistry and materials science. An international scientific authorship summarizes the state-of-the-art of the current research and gives an outlook on future developments.

Biotic stresses cause yield loss of 31-42% in crops in addition to 6-20% during post-harvest stage. Understanding interaction of crop plants to the biotic stresses caused by insects, bacteria, fungi, viruses, and oomycetes, etc. is important to develop resistant crop varieties. Knowledge on the advanced genetic and genomic crop improvement strategies including molecular breeding, transgenics, genomics-assisted breeding and the recently emerging genome editing for developing resistant varieties in vegetable crops is imperative for addressing FPNEE (food, health, nutrition. energy and environment) security. Whole genome sequencing of these crops followed by genotyping-by-sequencing have facilitated precise information about the genes conferring resistance useful for gene discovery, allele mining and shuttle breeding which in turn opened up the scope for 'designing' crop genomes with resistance to biotic stresses. The nine chapters each dedicated to a vegetable crop or crop-group in this volume will deliberate on different types of biotic stress agents and their effects on and interaction with crop plants; will enumerate on the available genetic diversity with regard to biotic stress resistance among available cultivars; illuminate on the potential gene pools for utilization in interspecific gene transfer; will brief on the classical genetics of stress resistance and traditional breeding for transferring them to their cultivated counterparts; will enunciate the success stories of genetic engineering for developing biotic stress resistant varieties; will discuss on molecular mapping of genes and QTLs underlying biotic stress resistance and their marker-assisted introgression into elite varieties; will enunciate on different emerging genomics-aided techniques including genomic selection, allele mining, gene discovery and gene pyramiding for developing resistant crop varieties with higher quantity and better quality; and will also elaborate some case studies on genome editing focusing on specific genes for generating disease and insect resistant crops.

Examples from various organs and diseases illustrate the potential benefit obtained when both therapeutic approaches are combined with delivery strategies. Representing the combined effort of several leading international research and clinical experts, this book, Emerging Trends in Cell and Gene Therapy, provides a complete account on and brings into sharp focus current trends and state-of-the-art in important areas at the interface of cell- and gene-based therapies. This book addresses the current fragmented understanding regarding these two research areas and fills the vast unmet educational need and interest of both students and researchers in academia and industry. Main features of the book: * Biological aspects of stem cell sources, differentiation and engineering. * Application of microfluidics to study stem cell dynamics * Potential clinical application of stem cells and gene therapy to specific human disease. * Utilization of biomaterials and stem cells in regenerative medicine with particular emphasis on spinal cord repair, ligament and bone tissue engineering. * Biomimetic multiscale topography for cell alignment.

Biomaterials for Clinical Applications is organized according to the World Health Organization 's report of the top 11 causes of death worldwide, and lays out opportunities for both biomaterials scientists and physicians to tackle each of these leading contributors to mortality. The introductory chapter discusses the global burden of disease. Each of the subsequent eleven chapters focuses on a specific disease process, beginning with the leading cause of death worldwide, cardiovascular disease. The chapters start with describing diseases where clinical needs are most pressing, and then envisions how biomaterials can be designed to address these needs, instead of the more technologically centered approached favored by most books in the field. This book, then, should appeal to chemical engineers and bioengineers who are designing new biomaterials for drug delivery and vaccine delivery, as well as tissue engineering.

Cotton, the most important natural fiber crop, has been improved by conventional breeding-largely through planned hybridization of different cotton genotypes, since the discovery of Mendelian genetics. All these efforts resulted in the development of resilient high yielding cotton varieties. However, the progress through conventional breeding procedures is slow because of long lag periods for developing a variety, little control over the new genetic combinations, unwanted traits and lack of foolproof performance testing system. Genomic assays discovered over the last two decades have made it possible to understand the "language" of the genome by associating the genes with specific traits. Together with the more recently established gene-editing tools like CRISPR-Cas9, the cotton genome can be tailored much more precisely than ever before. In this regard, genetic information has been harnessed, through (i) sequencing of the progenitor and cultivated cotton species, (ii) ongoing mega pan-genome sequencing projects, (iii) genetic and physical mapping, and (iv) introgression of genes from alien sources, that resulted in the development of resilient cotton cultivars. These technologies have been deployed or are attempting to overcome the challenges of water shortage, excessive heat in most cotton growing regions, infectious diseases and infestation of insect pests, as well as rising production cost, for sustainable cotton production beyond 2030. In this book, new knowledge generated by the cotton research community and its application for developing resilient cotton are comprehensively summarized. This book contributed by well-known cotton researchers is a timely collection of the challenges and successes of precision cotton breeding in a changing environment.

Valuation is a hot topic among life sciences professionals. There is no clear understanding on how to use the different valuation approaches and how to determine input parameters. Some do not value at all, arguing that it is not possible to get realistic and objective numbers out of it. Some claim it to be an art. In the following chapters we will provide the user with a concise val- tion manual, providing transparency and practical insight for all dealing with valuation in life sciences: project and portfolio managers, licensing executives, business developers, technology transfer managers, entrep- neurs, investors, and analysts. The purpose of the book is to explain how to apply discounted cash flow and real options valuation to life sciences p- jects, i.e. to license contracts, patents, and firms. We explain the fun- mentals and the pitfalls with case studies so that the reader is capable of performing the valuations on his own and repeat the theory in the exercises and case studies. The book is structured in five parts: In the first part, the introduction, we discuss the role of the players in the life sciences industry and their p- ticular interests. We describe why valuation is important to them, where they need it, and the current problems to it. The second part deals with the input parameters required for valuation in life sciences, i.e. success rates, costs, peak sales, and timelines.