This book offers a comprehensive collection of papers on CRISPR/Cas genome editing in connection with agriculture, climate-smart crops, food security, translational research applications, bioinformatics analysis, practical applications in cereals, floriculture crops, engineering plants for abiotic stress resistance, the intellectual landscape, regulatory framework, and policy decisions. Gathering contributions by internationally respected experts in the field of CRISPR/Cas genome editing, the book offers an essential guide for researchers, students, teachers and scientists in academia; policymakers; and public companies, private companies and cooperatives interested in understanding and/or applying CRISPR/Cas genome editing to develop new agricultural products.

This second edition volume provides an overview of some of the types of nanostructures commonly used in nanobiomedicine. The chapters in this book discuss practical information on the synthesis and characterization of a variety of solution-phase and surface-bound nanomaterials, with examples of how they can be used in sensing, imaging, and therapeutics. Specific topics include the synthesis and characterization of molecule and biomolecule-functionalized nanoconjugates with gold, iron oxide, or polymeric cores; the development of biosensing, imaging, and therapeutic applications of multicomponent/multifunctional nanostructures; and the application of flow cytometry in nanobiomedicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.< Thorough and comprehensive, Biomedical Nanotechnology: Methods and Protocols, Second Edition is a useful resource for scientists and researchers at all levels who are interested in working in a new area of nanoscience and technology, or in expanding their knowledge base in their current field.

The generation of tridimensional tissues, assembled from scaffolding materials populated with biologically functional cells, is the great challenge and hope of tissue bioengineering and regenerative medicine. The generation of biomaterials capable of harnessing the immune system has been particularly successful. This book provides a comprehensive view of how immune cells can be manipulated to suppresses inflammation, deliver vaccines, fight cancer cells, promote tissue regeneration or inhibit blood clotting and bacterial infections by functionally engineered biomaterials. However, long-lived polymers, such as those employed in orthopedic surgery or vascular stents, can often induce an immune reaction to their basic components. As a result, this book is also an important step towards coming to understand how to manipulate biomaterials to optimize their beneficial effects and downplay detrimental immune responses.

People working in development of drugs, pesticides, washing detergents, etc., are obliged by law to conduct analyses of the "metabolic pathways" or "maps" for the chemical compounds that they are using or proposing.
Everyone in these industries or carrying out research toward such products is therefore interested in having a reference on these compounds.
Key Features
* Covers literature relating to degradation and metabolic profiles of molecules
* Draws together the literature on degradation and metabolism patterns with that on diversified conditions of the environmental systems
* Indicates 3D chemical structures, predicted physico-chemical parameters, logP and the SMILES chemical notations of the parent compounds to assist scientists design the lead compounds for new discovery using computer-aided technology

This collection of 14 essays written by leading researchers on a variety of topics related to biotechnology focuses on the social, ethical, economic, legal, and political aspects of biotechnological applications. Usually defined as any technique that uses living organisms or processes to make or modify products, to improve plants or animals, or to develop micro-organisms, biotechnology has political characteristics similar to those of other technological advances, such as applications of artificial intelligence in manufacturing or new procedures in medicine. In all of these innovations, the rapid application of new scientific knowledge challenges existing social values, legal and political protections, and production processes. In presenting some of the technically and politically complex policy issues that need to be faced by local, state, and national-level policy makers, as well as academic, business, agricultural, and medical institutions during the next decade, these chapters anticipate an array of social, economic, and institutional consequences that will occur if biotechnology gains wide acceptance. The volume is divided into four sections that assess various facets of the biotechnological phenomenon. In Part I, biotechnology's social and political dimensions are probed in three chapters that examine the subject from three very different perspectives. American universities, agricultural cooperatives, and developing countries are the focus of Part II which investigates the response of institutions to biotechnological development. In Part III, biotechnology's potential impacts are gauged in three chapters that analyze economic and legal influences, inquire into the dairy industry and regulation of genetically engineered organisms, and evaluate regulatory experience with food safety. The final section is devoted to a presentation of public policy responses to biotechnology and includes four chapters that center on issue development and responsibilities, economic development and public policy as they relate to biotechnology, and a challenge to congressional policy makers and policy analysts. A real groundbreaker with substantial implications for the 21st century, this collection of essays must be read by all public policy makers and by scientists working to further biotechnological development. This work is suitable as a textbook in upper level and graduate courses in public policy, interdisciplinary biotechnology, and science and technology courses.

The 19th ESACT meeting was to highlight the novel capabilities of the industry to move the products towards the clinic and was attended by a wide range of workers in the industry and for many it was their first ESACT meeting. The meeting was started with a session on Transcription to Secretion with a notable set of presentations on the emerging issues. The other sessions that followed Therapeutic Cell Engineering, Gene Medicine, Cells to Tissue, Protein products and Process Technology guided the delegates through the advances made for the progression of the biotechnology towards the industrial application of the products from cells. The meeting was supported by some exceptional invited speakers from around the world whose contributions complemented the emerging technologies and the changes being made at the industrial end of the ESACT spectrum.

Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors focuses on direct measurement sensors, indirect methods, ligands, and related technologies, including methods involving electrochemical detectors, recognition ligands, antibodies, aptamers, and peptides, amongst many other subjects. Written in the highly successful Methods in Molecular Biologya" series format, chapters include brief introductions to the topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.

This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting interesting examples of these assays, organized according to the type of analytic methods (colorimetric, fluorescence, electrochemical, etc.) and detected objects (protein, nucleic acid, small-molecule, ion, etc.). It also includes a chapter discussing the introduction of sandwich assays as biosensors for the detection of a range of targets. It is an interesting and useful resource for a wide readership in various fields of chemical science and nanotechnology.

At the end of the 20th century, a tremendous progress was made in biotechnology in its widest sense. This progress was largely possible as a result of joint efforts of top academic researchers in both pure fundamental sciences and applied research. The surplus value of such interdisciplinary approaches was clearly highlighted during the 9th European Congress on Biotechnology that was held in Brussels, Belgium (11-15 July, 1999). The present volume in the 'Focus on Biotechnology' series, entiteld 'Physics and Chemistry Basis for Biotechnology' contains selected presentations from this meeting, A collection of experts has made serious efforts to present some of the latest developments in various scientific fields and to unveil prospective evolutions on the threshold of the new millenium. In all contributions the emphasis is on emerging new areas of research in which physicochemical principles form the foundation. In reading the different chapters, it appears that more than ever significant advances in biotechnology very often depend on breakthroughs in the biotechnology itself (e.g.

This book focuses on various types of bioactive compounds, including secondary metabolites, oligosaccharides, polysaccharides, flavonoids, peptides/proteins, carotenoid pigments, quinones, terpenes, and polyunsaturated fatty acids, and presents an overview of their nutraceutical activities. It covers the current status and future potential of food compounds, as well as extraction technologies for bioactives derived from plant, fungi and marine-derived bioactive agents. Finally, health-promoting effects of plant, fungi and marine-derived bioactive agents are discussed. Chapters come from top researchers in this area from around the globe. The volume caters to the needs of undergraduate and post-graduate students in the area of food biotechnology, food bioprocessing, biotechnology, food engineering, etc., and also contains information pertinent to researchers.

Biohydrogen is considered the most promising energy carrier and its utilization for energy storage is a timely technology. This book presents latest research results and strategies evolving from an international research cooperation, discussing the current status of Biohydrogen research and picturing future trends and applications.

This detailed volume provides background on recent new technology developments highlighting the potential of the genomic era in wheat breeding with invaluable instruction on the methodology, which is complemented by overview chapters on the status of new technology application in major wheat production countries. The topics, addressed by internationally renowned scientists active in the field, cover methods underpinning the latest developments in the field of wheat biotechnology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Wheat Biotechnology: Methods and Protocols serves as a vital resource for scientists working to breed future high-yielding wheat varieties to sustain a growing population in an increasingly unpredictable world.

Coronavirus Drug Discovery, Volume Two: Antiviral Agents from Natural Products and Nanotechnological Applications presents detailed information on drug discovery against COVID-19. Sections in this volume present chapters that focus on the various antiviral agents from natural products that have the propensity to be used as chemical scaffolds for the development of drugs against COVID-19. Also captured are the dietary sources of antioxidant bioactives that may help boost the immune system for the management of COVID-19. Other chapters describe the application of nanotechnology for efficient and effective delivery of drugs against COVID-19. Written by global team of experts, this book is an excellent resource for drug developers, medicinal chemists, pharmaceutical companies in R&D and research institutes in both academia and industry.

th We compiled this volume mostly from presentations at the 6 International Plant Cold Hardiness Seminar (PCHS) after consulting with Professor Tony H. H. Chen, Oregon State University, USA, Professor Pekka Heino, University of Helsinki, Finland, th and Dr. Gareth J. Warren, University of London, Surrey, UK. The 6 International PCHS was held at the Unitas Congress Center, Helsinki, Finland from July 1-5, 2001. There were 110 registered scientists at the serttinar representing 20 countries: Australia, Belgium, Canada, Chile, the Czech Republic, Denmark, Estonia, Finland, Gennany, Hungary, Iceland, Italy, Japan, Norway, Poland, Spain, Sweden, Taiwan, United Kingdom, and United States of America. The infonnation compiled represents the state of the art of research in phmt cold hardiness in tenns of gene regulation, gene expression, signal transduction, the physiology of cold hardiness and, ultimately, the genetic engineering for cold tolerant plants. The International PCHS was initiated in 1977 at the University of Minnesota, St. Paul, Minnesota. It has been traditionally held at 5-year intervals at various locations. th Because of the rapid advances of research in plant cold hardiness, attendees at the 6 meeting unanimously adopted a resolution to hold the seminar in 3-year intervals instead of 5 in the future. Consequently, the next seminar will be held in 2004 in Sapporo, Japan, and Professor Seizo Fujikawa from Hokkaido University will serve as the host.

Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Building Biotechnology helps readers start and manage biotechnology companies and understand the business of biotechnology. This acclaimed book describes the convergence of scientific, policy, regulatory, and commercial factors that drive the biotechnology industry and define its scope. In addition to its popularity among business professionals and scientists seeking to apply their skills to biotechnology, Building Biotechnology has also been adopted as a course text in dozens of advanced biotechnology programs. This fourth edition significantly expands upon the foundation laid by the first three, updating case law and business models in this dynamic industry and adding significantly more case studies, informative figures and tables. Most importantly, Building Biotechnology enables seasoned business professionals and entrepreneurial scientists alike to understand the drivers of biotechnology businesses and apply their established skills for commercial success.

"Cardiac Tissue Engineering: Methods and Protocols "presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe. These include methods and protocols for cell preparation, biomaterial preparation, cell seeding, and cultivation in various systems. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, "Cardiac Tissue Engineering: Methods and Protocols "highlights the major techniques, both experimental and computational, for the study of cardiovascular tissue engineering.

Arbuscular Mycorrhiza (AM) is the most common mycorrhizal type involved in agricultural systems, and the most widespread plant root symbiosis. The fungi involved (Glomales) are known to promote plant growth and health by acting as biofertilizers, bioprotectors and bioregulators. The main aim of this book is to provide readers with theoretical and applied knowledge essential for the use of AM fungi in improving plant health and fitness, production of high quality food and in conservation of natural resources. The different chapters target understanding the role of AM fungi in sustainable crop production, discussing ways to improve biological equilibria between microorganisms in the mycorrhizosphere, analysing genetic, physiological, cellular and molecular bases of AM functioning and establishing technologies for inoculum production, according to the regulatory guidelines for application.

Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Protocols, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 1: Optical-Based Detectors delves into direct and indirect optical detectors, including methods involving surface plasmon resonance, interferometric sensors, CCD based detectors, and spectrometers, among many other cutting-edge technologies. Written in the highly successful Methods in Molecular Biologya" series format, chapters include brief introductions to the subjects, lists of the necessary materials, step-by-step, readily reproducible protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Protocols is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.

The tobacco BY-2 cell system is a unique model cell line for the study of dynamic features of plant cells. As extension of Volume 53, Tobacco BY-2 Cells, which presented basic aspects of the cell system, this present volume provides a wealth of new approaches. Topics include: various aspects of the cell cycle and cellular dynamics using BY-2 cells; physiological and developmental aspects of BY-2 cells; recent developments in the knowledge of intracellular traffic of BY-2 cells; BY-2 cells as hosts for infectious diseases; dynamic features of mitochondrial fusion and division; BY-2 cells as tools to elucidate the biosynthesis of isoprenoids; recent developments in the omics of BY-2 cells; and novel techniques for handling BY-2 cells. This latest volume in the series is an invaluable source of information for scientists in basic and applied plant biology.

My journey into this fascinating field of biotechnology started about 26 years ago at a small biotechnology company in South San Francisco called Genentech. I was very fortunate to work for the company that begat the biotech industry during its formative years. This experience established a solid foundation from which I could grow in both the science and business of biotechnology. After my fourth year of working on Oyster Point Boulevard, a close friend and colleague left Genentech to join a start-up biotechnology company. Later, he approached me to leave and join him in of all places - Oklahoma. He persisted for at least a year before I seriously considered his proposal. After listening to their plans, the opportunity suddenly became more and more intriguing. Finally, I took the plunge and joined this ent- preneurial team in cofounding and growing a start-up biotechnology company. Making that fateful decision to leave the security of a larger company was extremely difficult, but it turned out to be the beginning of an entrepreneurial career that forever changed how I viewed the biotechnology industry. Since that time, I have been fortunate to have cofounded two other biotechnology com- nies and even participated in taking one of them public. During my career in these start-ups, I held a variety of positions, from directing the science, operations, regulatory, and marketing components, to subsequently becoming CEO.

This book discusses the recent innovations in the development of various advanced biopolymeric systems, including gels, in situ gels, hydrogels, interpenetrating polymer networks (IPNs), polyelectrolyte complexes (PECs), graft co-polymers, stimuli-responsive polymers, polymeric nanoparticles, nanocomposites, polymeric micelles, dendrimers, liposomes and scaffolds. It also examines their applications in drug delivery.

This book provides a comprehensive review of biosynthetic approaches to the production of industrially important chemicals and the environmental challenges involved. Its 19 chapters discuss different aspects of biosynthetic technology from the perspective of leading experts in the field. It covers various biorefinery approaches, including the use of microbes, metabolically engineered plants, biomass-based and green technology methods. Further, it examines important research in the areas of organic and hazardous waste composting, management and recovery of nutraceuticals from agro-industrial waste, biosynthesis and technological advancements of biosurfactants and waste water bioremediation. This book contributes to the scientific literature on biosynthetic technologies and the related environmental challenges for researchers and academics working in this area around the globe.