A number of books written by statisticians address the mathematical optimization of biological systems, but do not directly address statistical optimization. Statistical Optimization of Biological Systems covers the optimization of bioprocess systems in its entirety, devoting much-needed attention to the experimental optimization of biological systems using statistical techniques. Employing real-life bioprocess optimization problems and their solutions as examples, this book: Describes experimental design from identifying process variables to selecting a screening design, applying response surface methodology, and conducting regression modeling Demonstrates the statistical analysis and optimization of different experimental designs, the results of which are used to establish important variables and optimum settings Details the optimization techniques employed to determine optimum levels of the process variables for both single- and multiple-response systems Discusses important experimental designs, such as evolutionary operation programs and Taguchi's designs Delineates the concept of hybrid experimental design using the essence of a genetic algorithm Statistical Optimization of Biological Systems examines the complex nature of biological systems, the need for optimization, and the rationale of statistical and non-statistical optimization methods. More importantly, the book explains how to successfully apply mathematical and statistical techniques to the optimization of biological systems.

Plant cell cultures are used extensively in studies of secondary metabolism, for the biosynthesis of pharmaceuticals, flavors, essences, and pigments. This book highlights recent developments in the in vitro growth of cultured plant cells and in the production of valuable secondary metabolites.
Plant Cell Culture Secondary Metabolism details research on many exciting areas including:

This two-volume set will be useful to the established continuous culture operator, as well as the researcher, teacher, and student who is interested in learning how the technique of continuous culture could be useful in answering both basic and applied questions in microbiology and cell biology.

Marine Enzymes Biotechnology: Production and Industrial Applications, Part II - Marine Organisms Producing Enzymes provides a huge treasure trove of information on marine organisms. Nowadays, marine organisms are good candidates for enzymes production and have been recognized as a rich source of biological molecules that are of potential interest to various industries. Marine enzymes such as amylases, carboxymethylcellulases, proteases, chitinases, keratinases, xylanases, agarases, lipases, peroxidase and tyrosinases are widely used in the industry for the manufacture of pharmaceuticals, foods, beverages, and confectioneries, as well as in textile and leather processing, and in waste water treatment. The majority of the enzymes used in the industry are of microbial origin because microbial enzymes are relatively more stable than the corresponding enzymes derived from plants and animals.

Evidence-Based Validation of Herbal Medicines: Translational Research on Botanicals brings together current thinking and practice in the characterization and validation of natural products. The book describes different approaches and techniques for evaluating the quality, safety and efficacy of herbal medicine, particularly methods to assess their activity and understand compounds responsible and their probable underlying mechanisms of action. This book brings together the views, expertise and experiences of scientific experts in the field of medicinal plant research, hence it will be useful for researcher who want to know more about the natural lead with their validation and also useful to exploit traditional medicines.

This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing� of cells in various environments.

Definitive Treatment of the Numerical Simulation of Bioheat Transfer and Fluid Flow Motivated by the upwelling of current interest in subjects critical to human health, Advances in Numerical Heat Transfer, Volume 3 presents the latest information on bioheat and biofluid flow. Like its predecessors, this volume assembles a team of renowned international researchers who cover both fundamentals and applications. It explores ingenious modeling techniques and innovative numerical simulation for solving problems in biomedical engineering. The text begins with the modeling of thermal transport by perfusion within the framework of the porous-media theory. It goes on to review other perfusion models, different forms of the bioheat equation for several thermal therapies, and thermal transport in individual blood vessels. The book then describes thermal methods of tumor detection and treatment as well as issues of blood heating and cooling during lengthy surgeries. It also discusses how the enhancement of heat conduction in tumor tissue by intruded nanoparticles improves the efficacy of thermal destruction of the tumor. The final chapters focus on whole-body thermal models, issues concerning the thermal treatment of cancer, and a case study on the thermal ablation of an enlarged prostate.

This practical resource offers a concise guide to the combined use of classical and molecular methods for the genetic analysis and breeding of fungi - presenting basic concepts and experimental designs and demonstrating the power of fungal genetics for applied research in biotechnology and phytopathology. Addressing each major topic in the manipulation and analysis of fungi, Fungal Genetics explains genetic processes...examines spontaneous mutations, mutation induction, and the isolation and characterization of mutants...discusses sexual and parasexual genetic analysis...details physical karyotypes and restriction fragment analysis...describes the organization of genetic material in fungal nuclei as well as meiotic and mitotic processes...compares genetic mapping techniques and breeding strategies...and more. Written by over 25 international experts representing nine countries and containing nearly 1000 bibliographic citations, Fungal Genetics is an indispensable reference for mycologists, microbiologists, phytopathologists, biotechnologists, biochemists, molecular and cell biologists, geneticists, botanists, plant physiologists and pathologists, microbial and fungal ecologists, and upper-level undergraduate and graduate students in these disciplines.

In this unique book, experts describe practices applicable to the large-scale processing of biotechnological products. Beginning with processing and bulk storage preservation techniques, the book provides strategies for improving efficiency of process campaigns of multiple products and manufacturing facilities for such processing techniques. Large-scale chromatography for the purification of biomolecules in manufacturing and lyophilization of protein pharmaceuticals are discussed. Includes a case study on blow-fill-seal processing technology and a chapter on economic and cost factors for bioprocess engineering.

This book covers the structure and classification of adhesion molecules, immunohistochemical localization, junctional, functional and inflammatory adhesion molecules and molecular imaging. It discusses adhesion molecules in relation to signaling pathways, gene expression, arginine, stem cells, neutrophil migration, leukocytes decompression sickness, ischemia reperfusion injury, diabetes, obesity, metabolic syndrome, weight loss, hypoxia, kidney disease, smoking, osteoprotegerin, tumors, bioinformatics, high-throughput technologies, chemotherapy, hematopoiesis, lipoproteins, fatty acids, atrial fibrillation and heart disease, and the brain and dementia.

Because artificial sensors have assumed a major role in both domestic and industrial settings, the development of new technologies continues. Sensor Materials is the first publication that approaches the subject of sensors from a materials standpoint and provides a global overview of the field in a single, compact, and accessible volume.
Introductory chapters present a solid introduction to the classification of sensors and the various properties of materials used in sensing. The subsequent chapters deal with specific areas of sensor materials, including metal, semiconductor, dielectric, solid electrolytic, magnetic, fiber optics, radiant, and biological materials. In addition, there is substantial bibliographic information that provides a source of published research literature for each sensor.

High Throughput Formulation Development of Biopharmaceuticals: Practical Guide to Methods and Applications provides the latest developments and information on the science of stable and safe drug product formulations, presenting a comprehensive review and detailed description of modern methodologies in the field of formulation development, a process starting with candidate and pre-formulation screening in its early development phase and then progressing to the refinement of robust formulations during commercialization in the later phases of development. The title covers topics such as experiment design, automation of sample preparation and measurements, high-throughput analytics, stress-inducing methods, statistical analysis of large amounts of formulation study data, emerging technologies, and the presentation of several case studies, along with a concluding summary.

Advances in genomics and combinatorial chemistry during the past two decades inspired innovative technologies and changes in the discovery and pre-clinical development paradigm with the goal of accelerating the process of bringing therapeutic drugs to market. Written by William Kisaalita, one of the foremost experts in this field, 3D Cell-Based Biosensors in Drug Discovery Programs: Microtissue Engineering for High Throughput Screening provides the latest information - from theory to practice - on challenges and opportunities for incorporating 3D cell-based biosensors or assays in drug discovery programs. The book supplies a historical perspective and defines the problem 3D cultures can solve. It also discusses how genomics and combinatorial chemistry have changed the way drug are discovered and presents data from the literature to underscore the less-than-desirable pharmaceutical industry performance under the new paradigm. The author uses results from his lab and those of other investigators to show how 3D micro environments create cell culture models that more closely reflect normal in vivo-like cell morphology and function. He makes a case for validated biomarkers for three-dimensionality in vitro and discusses the advantages and disadvantages of promising tools in the search of these biomarkers. The book concludes with case studies of drugs that were abandoned late in the discovery process, which would have been discarded early if tested with 3D cultures. Dr. Kisaalita presents evidence in support of embracing 3D cell-based systems for widespread use in drug discovery programs. He goes to the root of the issue, establishing the 3D cell-based biosensor physiological relevance by comparing 2D and 3D culture from genomic to functional levels. He then assembles the bioengineering principles behind successful 3D cell-based biosensor systems. Kisaalita also addresses the challenges and opportunities for incorporating 3D cell-based biosensors or cultures in current discovery and pre-clinical development programs. This book makes the case for widespread adoption of 3D cell-based systems, rendering their 2D counterparts, in the words of Dr. Kisaalita "quaint, if not archaic" in the near future.

Colloids show great potential in a wide variety of applications, including drug delivery and medical imaging, and the design and fabrication of colloid systems has attracted considerable interest in the research community. Colloids in Biotechnology describes developments in the field of biotechnological applications in the past decade and bridges the gap between these research efforts and commercially viable options. Highlights the role of colloids in a plethora of biotechnical applications Striking a balance between theory and experiment, between principles and applications, and between molecular and physical approaches to the subject, the book assembles contributions from an international community of colloid scientists to provide a comprehensive reference on the role of colloids in biotechnology and biomedicine. The authors discuss new types of biosurfactants; mixtures of surfactants; and peptides, proteins, and polyelectrolytes. They also describe the formation and properties of magnetic colloids and review their applications in chemical biology and medicine. They highlight current progress in the design of self-assembled materials for biotechnology, and they also cover the formation of nanofibres and the use of sol-gel technology in biology. Contains contributions from a diverse team of researchers The chapter authors have been given the freedom to present the spectrum of the relevant science, from pure to applied, in their particular topic. The compilation of this vast experience makes this text a valuable reference for those working in research and development in a range of technologies as well as academic scientists in the colloid and surface science field.

This work present practical, biotechnological applications of flow cytometry techniques for the study of animal, plant and microbial cells, explaining methodologies for sample preparation, staining and analysis. It discusses cell variability in cell culture processes and shows how the quantitative analysis of heterogeneous populations aids in the biotechnological exploitation of cells.

Nanoparticles are considered to be the building blocks for nanotechnology and are referred to as the particles having more than one dimension of the order of 100 nm or less.The nanostructured materials are being offered as better built, long lasting, cleaner, safer, and smarter products for use in communications, medicine, transportation, agriculture and other industries. Topics in molecular recognition, biomolecule-nanocrystal conjugates as fluorescence label for biological cells, and DNA-mediated groupings of nanocrystals are widespread, intriguing researchers from both biological and engineering fields. The diversity of nanotechnology covers fields from biology to material science, physics to chemistry, and other fields with variety of specialties. Controlled size, shape, composition, crystallinity, and structure-dependent properties of nanoparticles govern the unique properties of nanotechnology. The controlled biosynthesis of nanoparticles is of high scientific and technological interest as the microorganisms grab target ions from their environment and then turn the metal ions into the element metal through enzymatic mechanism generated through their cellular ("Intra"/ "Extra") activities.

The project aims to introduce the basics and advancements made so far in the field of biosynthesis of nanoparticles for graduate students and researchers around the world. The main aims are to (a) introduce the reader to the variety of microorganisms and their ability to synthesize the nanoparticles, (b) provide an overview of the methodologies applied to biosynthesize the variety of nanoparticles of medical and commercial uses, (c) provide a literature review on diversity of microorganisms able to synthesize nanoparticles of different types, (d) to discuss the regulatory mechanisms in microorganism able to synthesize variety of nanoparticles, (e) discuss experimental design problems associated with the controlled biosynthesis of nanoparticles, (f) discuss the stability and toxicity of nanoparticles in varying environment towards their therapeutic implications. The regulations, challenges and implications of biosynthesized nanoparticles for commercial significance will also represent among the main sections of the book. These aims will be organized by invited research/ review articles from renowned researchers exploring biosynthesis of variety of nanoparticles, and differ in length and number of chapters, with the literature review section containing the bulk of the text.

A thorough overview of nanobiotechnology and its place in advances in applied science and engineering, The Nanobiotechnology Handbook combines contributions from physics, bioorganic and bioinorganic chemistry, molecular and cellular biology, materials science, and medicine as well as from mechanical, electrical, chemical, and biomedical engineering to address the full scope of current and future developments. World-class experts discuss the role of nanobiotechnology in bioanalysis, biomolecular and biomedical nanotechnology, biosensors, biocatalysis and biofuel, and education and workforce development. It includes downloadable resources that contain all figures in the book. The book begins with discussions of biomimetic nanotechnology, including a comprehensive overview of DNA nanostructure and DNA-inspired nanotechnology, aptamer-functionalized nanomaterials as artificial antibodies, artificial enzymes, molecular motors, and RNA structures and RNA-inspired nanotechnology. It shows how nanotechnology can be inspired by nature as well as adverse biological events in diagnostic and therapeutic development. From there, the chapters cover major important and widely used nanofabrication techniques, applications of nanotechnology for bioprocessing followed by coverage of the applications of atomic force microscopy (AFM), optical tweezers and nanofluidics as well as other nanotechnology-enabled biomolecular and cellular manipulation and detection. Focusing on major research trends, the book highlights the importance of nanobiotechnology to a range of medical applications such as stem cell technology and tissue engineering, drug development and delivery, imaging, diagnostics, and therapeutics. And with coverage of topics such as nanotoxicity, responsible nanotechnology, and educational and workforce development, it provides a unique overview and perspective of nanobiotechnology impacts from a researcher's, entrepreneur's, economist's and educator's point of view. It provides a resource for current applications and future development of nanobiotechnology.

Tissue engineering research continues to captivate the interest of researchers and the general public alike. Popular media outlets like The New York Times, Time, and Wired continue to engage a wide audience and foster excitement for the field as regenerative medicine inches toward becoming a clinical reality. Putting the numerous advances in the field into a broad context, Tissue Engineering: Principles and Practices explores current thoughts on the development of engineered tissues. With contributions from experts and pioneers, this book begins with coverage of the fundamentals, details the supporting technology, and then elucidates their applications in tissue engineering. It explores strategic directions, nanobiomaterials, biomimetics, gene therapy, cell engineering, and more. The chapters then explore the applications of these technologies in areas such as bone engineering, cartilage tissue, dental tissue, vascular engineering, and neural engineering. A comprehensive overview of major research topics in tissue engineering, the book: Examines the properties of stem cells, primary cells, growth factors, and extracellular matrix as well as their impact on the development of tissue-engineered devices Focuses upon those strategies typically incorporated into tissue-engineered devices or utilized in their development, including scaffolds, nanocomposites, bioreactors, drug delivery systems, and gene therapy techniques Presents synthetic tissues and organs that are currently under development for regenerative medicine applications The contributing authors are a diverse group with backgrounds in academia, clinical medicine, and industry. Furthermore, this book includes contributions from Europe, Asia, and North America, helping to broaden the views on the development and application of tissue-engineered devices. The book provides a useful reference for courses devoted to tissue engineering fundamentals and those laboratories developing tissue-engineered devices for regenerative medicine therapy.

Nanostructured materials with multiple components and complex structures are the current focus of research and are expected to develop further for material designs in many applications in electrochemical, colloidal, medical, pharmaceutical, and several other fields. This book discusses complex nanostructured systems exemplified by nanoporous silicates, spontaneously formed gels from silica-nanocolloidal solutions, and related systems, and examines them using molecular dynamics simulations. Nanoporous materials, nanocolloidal systems, and gels are useful in many applications and can be used in electric devices and storage, and for gas, ion, and drug delivery. The book gives an overview of the history, current status, and frontiers of the field. It also discusses the fundamental aspects related to the common behaviors of some of these systems and common analytical methods to treat them.

Gleaning information from more than 100 experts in the field of cancer diagnosis, prognosis, and therapy worldwide, Cancer Biomarkers: Non-Invasive Early Diagnosis and Prognosis determines the significance of clinical validation approaches for several markers. This book examines the use of noninvasive or minimally invasive molecular cancer markers that are under development or currently in use. It deals with a majority of commonly prevalent cancers and can help anyone working in the health-care industry to recommend or develop early diagnostics, at-risk tests, and prognostic biomarkers for various cancers. It explores the practice of determining biomarkers by their characteristics and relative methodologies, and presents the most recent data as well as a number of current and upcoming early diagnostic noninvasive molecular markers for many common cancers. It also considers the sensitivity and specificity of markers, biomarker market, test providers, and patent information. Approximately 30-35 Cancer Specific Noninvasive Molecular Diagnostic Markers in a Single Volume The book details the general and technical aspects of noninvasive cancer markers. It covers imaging, cutting-edge molecular technologies for biomarker development, and noninvasive or minimally invasive sources of molecular markers, as well as quality control and ethical issues in cancer biomarker discovery. It also provides a detailed account of brain, head and neck, and oral cancer markers, and provides information on a number of gastrointestinal cancers, lung cancer, and mesothelioma markers. Emphasizes the Importance of Volatile Markers in Early Cancer Diagnosis Presents noninvasive early molecular markers in urological cancers Describes gynecological and endocrine cancer markers Details noninvasive markers of breast, ovarian, cervical, and thyroid cancers Addresses hematological malignancies Contains information on noninvasive molecular markers in myelodysplastic syndromes, acute myeloid leukemia, Hodgkin's lymphoma, and multiple myeloma Provides comprehensive information on diagnostic and prognostic biomarkers in cutaneous melanoma This text considers molecular technologies for biomarker development, noninvasive or minimally invasive sources of molecular markers, and quality control and ethical issues in cancer biomarker discovery.

Oceans are an abundant source of diverse biomaterials with potential for an array of uses. Marine Biomaterials: Characterization, Isolation and Applications brings together the wide range of research in this important area, including the latest developments and applications, from preliminary research to clinical trials. The book is divided into four parts, with chapters written by experts from around the world. Biomaterials described come from a variety of marine sources, such as fish, algae, microorganisms, crustaceans, and mollusks. Part I covers the isolation and characterization of marine biomaterials-bioceramics, biopolymers, fatty acids, toxins and pigments, nanoparticles, and adhesive materials. It also describes problems that may be encountered in the process as well as possible solutions. Part II looks at biological activities of marine biomaterials, including polysaccharides, biotoxins, and peptides. Chapters examine health benefits of the biomaterials, such as antiviral activity, antidiabetic properties, anticoagulant and anti-allergic effects, and more. Part III discusses biomedical applications of marine biomaterials, including nanocomposites, and describes applications of various materials in tissue engineering and drug delivery. Part IV explores commercialization of marine-derived biomaterials-marine polysaccharides and marine enzymes-and examines industry perspectives and applications. This book covers the key aspects of available marine biomaterials for biological and biomedical applications, and presents techniques that can be used for future isolation of novel materials from marine sources.

This is a review of what needs to be done to realize the potential of monoclonal antibodies. It assesses the competing technologies with advice on the best approach for a particular situation. Monoclonal antibodies have revolutionized immunology and are now promising to have a similar impact on clinical medicine. Recent developments should overcome many of the difficulties experienced in the past which has seen a great deal of talk about the therapeutic applications of monoclonal antibodies, but very little routine therapy.

Analysis of the structure and organization of the human genome is proceeding apace, bringing with it new insights into its function. This volume is a review of the relationship between structure and function in the human genome, and a detailed description of some of the important methdologies for unravelling the function of genes and genomic structures. Analysis of the structure and organization of the human genome is proceeding apace, bringing with it new insights into its function.

Addressing medium- and long-term expectations for human health, this book reviews current scientific and technical developments in nanotechnology for biomedical, agrofood, and environmental applications. This collection of perspectives on the ethical, legal, and societal implications of bionanotechnology provides unique insight into contemporary technological developments. Readers with a technical background will benefit from the overview of the state-of-the-art research in their field, while readers with a social science background will benefit from the discussion of realistic prospects of nanotechnology. The text also includes a glossary as well as extensive end-of-chapter references.

Nanoscale techniques and devices have had an explosive influence on research in life sciences and bioengineering. Reflecting this influence, Nanopatterning and Nanoscale Devices for Biological Applications provides valuable insight into the latest developments in nanoscale technologies for the study of biological systems. Written and edited by experts in the field, this first-of-its-kind collection of topics: Covers device fabrication methods targeting the substrate on the nanoscale through surface modification Explores the generation of nanostructured biointerfaces and bioelectronics elements Examines microfluidically generated droplets as reactors enabling nanoscale sample preparation and analysis Gives an overview of key biosensors and integrated devices with nanoscale functionalities Discusses the biological applications of nanoscale devices, including a review of nanotechnology in tissue engineering Readers gain a deep understanding of the cutting-edge applications of nanotechnologies in biological engineering, and learn how to apply the relevant scientific concepts to their own research. Nanopatterning and Nanoscale Devices for Biological Applications is the definitive reference for researchers in engineering, biology, and biomedicine, and for anyone exploring the newest trends in this innovative field.