Baculovirus Expression Systems and Biopesticides Edited by Michael L. Shuler, H. Alan Wood, Robert R. Granados, and Daniel A. Hammer Baculovirus Expression Systems and Biopesticides provides an integrated perspective on the use of the continually evolving baculovirus-insect cell system in the production of recombinant proteins and genetically engineered pesticides. Divided into three main sections--Developing Effective Virus-Insect Culture Systems, Bioreactor Design and Scale-Up Issues, and Commercial Application of Insect Cell Culture--the book, written by highly regarded editors in the field, describes:
* The molecular biology and genetics of baculoviruses
* The use of baculoviruses as expression systems
* Principles and methods for small- and large-scale insect cultures
* The use of wild type and genetically engineered baculoviruses as viral pesticides
The breadth of coverage in Baculovirus Expression Systems and Biopesticides will meet the needs of molecular biologists, chemical engineers, biotechnologists, virologists, and entomologists.

This book presents a comprehensive study on microextrusion-based 3D bioprinting technologies for bioinks with various crosslinking mechanisms, chiefly focusing on the bioprinting process and bioink properties to provide readers with a better understanding of this state-of-the-art technology. Further, it summarizes a number of general criteria and research routes for microextrusion-based 3D bioprinting using three experimental studies based on shear-thinning, thermo-sensitive and non-viscous hydrogel bioinks. The book also presents sample applications in the areas of stem cells and cell matrix interaction. The book highlights pioneering results in the development of bioprinting technologies and bioinks, which were published in high-quality journals such as Advanced Materials, Biofabrication and ACS Biomaterials Science & Engineering. These include an in-situ crosslinking strategy that overcomes the viscosity limits for bioinks, which is virtually impossible using conventional strategies, and can be generalized for other bioink formulations.

This book examines how biotechnology can improve livestock breeding and farming, and thereby also animal products. In the first chapters the reader will discover which techniques and approaches are currently used to improve animal breeding, animal health and the value of animal products. Particular attention is given to reproduction techniques, animal nutrition and livestock vaccines that not only enhance animal health but also have a significant effect on human health by ensuring safe food procurement and preventing zoonotic diseases. In addition, modern biotechnology can increase not only productivity but also the consistency and quality of animal food, fiber and medical products. In the second part of the book, issues such as how animal biotechnology could affect the environment and the important topic of animal waste management are explored. In the concluding chapter, the authors discuss future challenges related to animal biotechnology. This work will appeal to a wide readership, from scientists and professionals working in animal production, to those in farm animal management and veterinary science.

"Biomarkers and Biosensors" offers thorough coverage of biomarker/biosensor interaction, current research trends, and future developments in applications of drug discovery. This book is useful to researchers in this field as well as clinicians interested in new developments in early detection and diagnosis of disease or the mode of operation of biomarkers. "Biomarkers and Biosensors" also emphasizes kinetics, and clearly delineates how this influences the biomarker market.
Offersthorough coverage of the kinetics of biomarker interaction with the biosensor surfaceProvides evidence-based approach to evaluate effectivenessProvides pharmaceutical chemists the possibilities and methodology in assessing the effectiveness of new drugsProvides the information needed for the selection of the best biomarker for a specific application"

Basic Laboratory Methods for Biotechnology, Third Edition is a versatile textbook that provides students with a solid foundation to pursue employment in the biotech industry and can later serve as a practical reference to ensure success at each stage in their career. The authors focus on basic principles and methods while skillfully including recent innovations and industry trends throughout. Fundamental laboratory skills are emphasized, and boxed content provides step by step laboratory method instructions for ease of reference at any point in the students' progress. Worked through examples and practice problems and solutions assist student comprehension. Coverage includes safety practices and instructions on using common laboratory instruments. Key Features: Provides a valuable reference for laboratory professionals at all stages of their careers. Focuses on basic principles and methods to provide students with the knowledge needed to begin a career in the Biotechnology industry. Describes fundamental laboratory skills. Includes laboratory scenario-based questions that require students to write or discuss their answers to ensure they have mastered the chapter content. Updates reflect recent innovations and regulatory requirements to ensure students stay up to date. Tables, a detailed glossary, practice problems and solutions, case studies and anecdotes provide students with the tools needed to master the content. To succeed in the lab, it is crucial to be comfortable with the math calculations that are part of everyday work. This accessible introduction to common laboratory techniques focuses on the basics, helping even readers with good math skills to practice the most frequently encountered types of problems. Basic Laboratory Calculations for Biotechnology, Second Edition discusses very common laboratory problems, all applied to real situations. It explores multiple strategies for solving problems for a better understanding of the underlying math. Primarily organized around laboratory applications, the book begins with more general topics and moves into more specific biotechnology laboratory techniques at the end. This book features hundreds of practice problems, all with solutions and many with boxed, complete explanations; plus hundreds of "story problems" relating to real situations in the lab. Additional features include: Discusses common laboratory problems with all material applied to real situations Presents multiple strategies for solving problems help students to better understand the underlying math Provides hundreds of practice problems and their solutions Enables students to complete the material in a self-paced course structure with little teacher assistance Includes hundreds of "story problems"that relate to real situations encountered in the laboratory

Synthetic biology is a new area of biological research that combines science and engineering in order to design and build novel biological functions and systems. The definition of synthetic biology has been generally accepted as the engineering of biology: the synthesis of complex, biologically based (or inspired) systems, which display functions that do not exist in nature. This engineering perspective may be applied at all levels of the hierarchy of biological structures from individual molecules to whole cells, tissues and organisms. As with any multi-disciplinary field, there is an immense and rapidly-growing body of literature concerning synthetic biology, with several dedicated journals now available. However, locating the best information, or identifying the hottest topics can be time-consuming. This Specialist Periodical Report presents critical and comprehensive reviews of the recent literature in themed chapters prepared by invited authors from across the globe. The series editors are active in the field, ensuring that the most valuable information is presented in an authoritative manner.

Pretreatment of Biomass provides general information, basic data, and knowledge on one of the most promising renewable energy sources- biomass for their pretreatment- which is one of the most essential and critical aspects of biomass-based processes development. The quest to get the environment greener, less polluted, and less hazardous has led to the concept of Biorefineries growing fast globally for developing bio-based processes and products using biomass as the feedstock. Each kind of biomass requires some kind of pretreatment to make it suitable for bioprocess. This book provides state-of-art information on the methods currently available for this.

This book provides data-based scientific information on the most advanced and innovative processing for the pretreatment of lignocellulosic and algal biomass for further processing. Pretreatment of biomass is considered one of the most expensive steps in the overall processing in a biomass-to-biofuel program. With the strong advancement in developing lignocellulose biomass- and algal biomass-based biorefineries, global focus has been on developing pretreatment methods and technologies which could offer technical and economic feasibility. This book provides a comprehensive overview of the latest developments on different methods used for the pretreatment of biomass. A separate section is devoted to the methods and technologies of algal biomass due to the increasing global attention of its use.
Provides information on the most advanced and innovative pretreatament processes and technologies for biomassCovers information on lignocellulosic and algal biomass to work on the principles of biorefineryUseful for researchers intending to study scale-upProvides information on integration of processes and technologies for the pretreatment of biomass

From Dolly the sheep to Frankenfood, life-saving medicines, and beyond, this insightful work describes the technology and controversy behind genetic engineering. From the publication of Aldous Huxley's Brave New World in 1932 to the cloning of Dolly the sheep in 1996, the public has long been fascinated by the idea that humans may one day be able to mold or even create life. In less than 30 years, genetic engineering has itself mutated from science fiction to science fact. Supporters claim such innovations as genetically modified crops and gene therapy are poised to bring unparalleled benefits by eliminating hunger and hereditary disease, whereas critics warn the dream could easily become a nightmare. Packed with key facts and analysis, Genetic Engineering: A Reference Handbook, Second Edition provides an expert guide to the very latest discoveries in genetic engineering and genetic modification and the technology's complex ethical, scientific, and economic implications. Includes an expert guide to print and online resources on genetic engineering and related areas Features a comprehensive glossary designed for the general reader

Plant genomics and biotechnology have recently made enormous strides, and hold the potential to benefit agriculture, the environment and various other dimensions of the human endeavor. It is no exaggeration to claim that the twenty-first century belongs to biotechnology. Knowledge generation in this field is growing at a frenetic pace, and keeping abreast of the latest advances and calls on us to double our efforts. Volume II of this two-part series addresses cutting-edge aspects of plant genomics and biotechnology. It includes 37 chapters contributed by over 70 researchers, each of which is an expert in his/her own field of research. Biotechnology has helped to solve many conundrums of plant life that had long remained a mystery to mankind. This volume opens with an exhaustive chapter on the role played by thale cress, Arabidopsis thaliana, which is believed to be the Drosophila of the plant kingdom and an invaluable model plant for understanding basic concepts in plant biology. This is followed by chapters on bioremediation, biofuels and biofertilizers through microalgal manipulation, making it a commercializable prospect; discerning finer details of biotic stress with plant-fungal interactions; and the dynamics of abiotic and biotic stresses, which also figure elsewhere in the book. Breeding crop plants for desirable traits has long been an endeavor of biotechnologists. The significance of molecular markers, marker assisted selection and techniques are covered in a dedicated chapter, as are comprehensive reviews on plant molecular biology, DNA fingerprinting techniques, genomic structure and functional genomics. A chapter dedicated to organellar genomes provides extensive information on this important aspect. Elsewhere in the book, the newly emerging area of epigenetics is presented as seen through the lens of biotechnology, showcasing the pivotal role of DNA methylation in effecting permanent and transient changes to the genome. Exclusive chapters deal with bioinformatics and systems biology. Handy tools for practical applications such as somatic embryogenesis and micropropagation are included to provide frontline information to entrepreneurs, as is a chapter on somaclonal variation.Overcoming barriers to sexual incompatibility has also long been a focus of biotechnology, and is addressed in chapters on wide hybridization and hybrid embryo rescue. Another area of accomplishing triploids through endosperm culture is included as a non-conventional breeding strategy. Secondary metabolite production through tissue cultures, which is of importance to industrial scientists, is also covered. Worldwide exchange of plant genetic material is currently an essential topic, as is conserving natural resources in situ. Chapters on in vitro conservation of extant, threatened and other valuable germplasms, gene banking and related issues are included, along with an extensive account of the biotechnology of spices - the low-volume, high-value crops. Metabolic engineering is another emerging field that provides commercial opportunities. As is well known, there is widespread concern over genetically modified crops among the public. GM crops are covered, as are genetic engineering strategies for combating biotic and abiotic stresses where no other solutions are in sight. RNAi- and micro RNA- based strategies for crop improvement have proved to offer novel alternatives to the existing non-conventional techniques, and detailed information on these aspects is also included. The book's last five chapters are devoted to presenting the various aspects of environmental, marine, desert and rural biotechnology. The state-of-the-art coverage on a wide range of plant genomics and biotechnology topics will be of great interest to post-graduate students and researchers, including the employees of seed and biotechnology companies, and to instructors in the fields of plant genetics, breeding and biotechnology.

This volume is comprised of 18 chapters, covering various aspects of DNA modification and RNA modified bases. It also discusses in detail circular RNA, therapeutic oligonucleotides and their different properties. The chemical nature of DNA, RNA, protein and lipids makes these macromolecules easily modifiable, but they are also susceptible to damage from both endogenous and exogenous agents. Alkylation and oxidation show a potential to disrupt the cellular redox equilibrium and cause cellular damage leading to inflammation and even chronic disease. Furthermore, DNA damage can drive mutagenesis and the resulting DNA sequence changes can induce carcinogenesis and cancer progression. Modified nucleosides can occur as a result of oxidative DNA damage and RNA turnover, and are used as markers for various diseases. To function properly some RNA needs to be chemically modified post-transcriptionally. Dysregulation of the RNA-modification pattern or of the levels of the enzymes that catalyze these modifications alters RNA functionality and can result in complex phenotypes, likely due to defects in protein translation. While modifications are best characterized in noncoding ribonucleic acids like tRNA and rRNA, coding mRNAs have also been found to contain modified nucleosides. This book is a valuable resource, not only for graduate students but also researchers in the fields of molecular medicine and molecular biology.

This book reviews efforts to produce chemicals and fuels from forest and plant products, agricultural residues and more. Algae can potentially capture solar energy and atmospheric CO2; the book details needed research and legislative initiatives.

Bone substitute biomaterials are fundamental to the biomedical sector, and have recently benefitted from extensive research and technological advances aimed at minimizing failure rates and reducing the need for further surgery. This book reviews these developments, with a particular focus on the desirable properties for bone substitute materials and their potential to encourage bone repair and regeneration.

Part I covers the principles of bone substitute biomaterials for medical applications. One chapter reviews the quantification of bone mechanics at the whole-bone, micro-scale, and non-scale levels, while others discuss biomineralization, osteoductivization, materials to fill bone defects, and bioresorbable materials. Part II focuses on biomaterials as scaffolds and implants, including multi-functional scaffolds, bioceramics, and titanium-based foams. Finally, Part III reviews further materials with the potential to encourage bone repair and regeneration, including cartilage grafts, chitosan, inorganic polymer composites, and marine organisms.
Provides a detailed and accurate overview of the bone substitute biomaterials, a fundamental part of the biomaterials and biomedical sectorProvides readers with the principles of bone substitute biomaterialsReviews biomaterials for bone regeneration

The existence of life at high temperatures is quiet fascinating. At elevated temperatures, only microorganisms are capable of growth and survival. A variety of microbes survive and grow at such high temperatures. Many thermophilic microbial genera have been isolated from man-made (washing machines, factory effluents, waste streams and acid mine effluents) and natural (volcanic areas, geothermal areas, terrestrial hot springs, submarine hydrothermal vents, geothermally heated oil reserves and oil wells, sun-heated litter and soils/sediments) thermal habitats throughout the world. Both culture-dependent and culture-independent approaches have been employed for understanding the diversity of microbes in hot environments. These organisms not only tolerate such high temperatures but also usually require these for their growth and survival. They are known as thermophiles/thermophilic microbes, which include a wide variety of prokaryotes (Bacteria and Archaea) as well as eukaryotes (Fungi, Algae, Protozoa). Interest in their diversity, ecology, and physiology has increased enormously during the past few decades as indicated by the deliberations in international conferences on extremophiles and thermophiles every alternate year. The Phylogenetic relationship of the known microorganisms indicates the presence of thermophilic microorganisms at the position close to the Last Universal Common Ancestor (LUCA). It is widely accepted that metal-reducing microorganisms have a large impact on the geochemistry of subsurface environments through the cycling of metals and organic matter, and thereby affect water quality and taste. Furthermore, metal-reducing micro-organisms have potential applications in bioremediation, mineral leaching and energy generation processes and are of evolutionary interest as metal reduction is considered to be a very ancient form of respiration. Protein characterization surprisingly indicated that possible additional functionality and alternate site promiscuity could contribute to the diverse biochemical abilities of the bacteria, especially with respect to microbe-metal interactions. Thermophilic bacteria are also able to reduce a wide spectrum of other metals including Mn (IV), Cr (VI), U (VI), Tc (VII), Co (III), Mo (VI), Au (I, III), and Hg (II) which can be used for immobilization of toxic metals/radionuclides, e.g. for the bioremediation of hot waste water of disposal sites of radioactive wastes having temperature range favorable for thermophiles for a long period of time. The main sources of CO in hot environments inhabited by anaerobic thermophiles are volcanic exhalations and thermal degradation of organic matter. A number of phylogenetically diverse anaerobic prokaryotes, both Bacteria and Archaea, are known to metabolize CO. CO transformation may be coupled to methanogenesis, acetogenesis, hydrogenogenesis, sulfate or ferric iron reduction. The key enzyme of anaerobic CO utilization, the Ni-containing CO dehydrogenase, is synthesized in hydrogenogens as an enzyme complex with the energy-converting hydrogenase. The genomic analysis shows this enzymatic complex to be encoded by a single gene cluster. Themophilic moulds and bacteria have been extensively studied in plant biomass bioconversion processes, as sources of industrial enzymes and as gene donors for the heterologous expression of thermostable enzymes. In the development of third generation biofuels such as bioethanol, thermophilic fungal and bacterial enzymes are of particular interest. The entire genomes of several thermophilic bacteria and archaea have already been sequenced. The analysis of the genomic data provided resources for novel and useful proteins and enzymes. The entire genomic data have also provided specific feature of microbes and important information on the evolution of thermophilic microorganisms. In some thermophilic archaea, multiple types of chaperonins have been identified. The chaperonins have been found to change according to the environmental conditions, suggesting that the mechanism for maintaining correct structure of thermostable proteins in the thermophilic archaea is regulated by changing chaperonine molecules. These organisms have evolved several structural and chemical adaptations, which allow them to survive and grow at elevated temperatures. Thermostable enzymes play an important role in the biosynthesis of fine chemicals. They are generally more robust against the conditions of industrial biocatalysis utilized by the industry, which can be solvent based or at elevated temperatures. Many non-natural industrially interesting substrates are often not soluble under aqueous conditions and at ambient temperatures. The thermophilic Archaea are a good source of these enzymes, which have been cloned and over-expressed in Escherichia coli. These include alcohol dehydrogenases for chiral alcohol production, aminoacylases for optically pure amino acids and amino acid analogues, transaminases for chiral amine production and gamma lactamases for chiral gamma lactam building blocks which are subsequently incorporated into carbocyclic nucleotides. Considerable interest has been generated in the mechanism that nature utilizes to increase the stability of enzymes found in thermophilic and hyperthermophilic species. A comparative approach has been used to carry out a detailed study of specific enzymes from a range of organisms in order to understand acquired stability at a structural level. A directed or site-specific mutagenesis approach has been used for stabilizing mesophilic proteins. The specific mutations have been introduced by looking at the most primitive forms of life, which are thought to have evolved in a thermophilic environment. The book is aimed at bringing together scattered up-to-date information on various aspects of thermophiles such as the diversity of thermophiles and viruses of thermophiles, their potential roles in pollution control and bioremediation, composting and microb

Transport phenomena in porous media continues to be a field which attracts intensive research activity. This is primarily due to the fact that it plays an important and practical role in a large variety of diverse scientific applications. "Transport Phenomena in Porous Media II" covers a wide range of the engineering and technological applications, including both stable and unstable flows, heat and mass transfer, porosity, and turbulence.

"Transport Phenomena in Porous Media II" is the second volume in a series emphasising the fundamentals and applications of research in porous media. It contains 16 interrelated chapters of controversial, and in some cases conflicting, research, over a wide range of topics. The first volume of this series, published in 1998, met with a very favourable reception. "Transport Phenomena in Porous Media II" maintains the original concept including a wide and diverse range of topics, whilst providing an up-to-date summary of recent research in the field by its leading practitioners.

Volumes in this widely revered series present comprehensive reviews of drug substances and additional materials, with critical review chapters that summarize information related to the characterization of drug substances and excipients. This organizational structure meets the needs of the pharmaceutical community and allows for the development of a timely vehicle for publishing review materials on this topic.
The scope of the Profiles series encompasses review articles and database compilations that fall within one of the following six broad categories: Physical profiles of drug substances and excipients; Analytical profiles of drug substances and excipients; Drug metabolism and pharmacokinetic profiles of drug substances and excipients; Methodology related to the characterization of drug substances and excipients; Methods of chemical synthesis; and Reviews of the uses and applications for individual drug substances, classes of drug substances, or excipients.

Key features:

* Contributions from leading authorities * Informs and updates on all the latest developments in the field

Six years after its first edition, "Computed Tomography: Principles, Design, Artifacts, and Recent Advances, Second Edition" provides and updated overview of the evolution of CT, the mathematical and physical aspects of the technology, and the fundamentals of image reconstruction algorithms. Given the high visibility and public awareness of the impact of x-ray radiation, the second edition features a new chapter on x-ray dose and presents different dose reduction techniques ranging from patient handling, optimal data acquisition, image reconstruction, and postprocess. Based on the advancements over the past six years, the second edition includes new sections on cone beam reconstruction algorithms, nonconventional helical acquisition and reconstruction, new reconstruction approaches, and dual-energy CT. Finally, new to this edition is a set of problems for each chapter, providing opportunities to enhance reader comprehension and practice the application of covered material.

This book gives a comprehensive overview of electrochemical-based biosensors and their crucial components. Practical examples are given throughout the text to illustrate how the performance of electrochemical-based biosensors can be improved by nanoscale surface modification and how an optimal design can be achieved. All essential aspects of biosensors are considered, including electrode functionalization, efficiency of the mass transport of reactive species, and long term durability and functionality of the sensor. This book also: * Explains how the performance of an electrochemical-based biosensor can be improved by nanoscale surface modification * Gives readers the tools to evaluate and improve the performance of a biosensor with a multidisciplinary approach that considers electrical, electrostatic, electrochemical, chemical, and biochemical events * Links the performance of a sensor to the various governing physical and chemical principles so readers can fully understand how a biosensor with nanoscale modified electrode surface functions.

Ion-exchange Technology I: Theory and Materials describes the theoretical principles of ion-exchange processes. More specifically, this volume focuses on the synthesis, characterization, and modelling of ion-exchange materials and their associated kinetics and equilibria. This title is a highly valuable source not only to postgraduate students and researchers but also to industrial R&D specialists in chemistry, chemical, and biochemical technology as well as to engineers and industrialists.

"Analysis, Removal, Effects and Risk of Pharmaceuticals in the Water Cycle" provides an overview of the current analytical methods for trace determination of pharmaceuticals in environmental samples. The book also reviews the fate and occurrence of pharmaceuticals in the water cycle for their elimination in wastewater and drinking water treatment, focusing on the newest developments in treatment technologies, such as membrane bioreactors and advanced oxidation processes.

Pharmaceutically active substances are a class of new, so-called emerging contaminants that have raised great concern in recent years. Human and veterinary drugs are continuously being released into the environment mainly as a result of the manufacturing processes, the disposal of unused or expired products, and via excreta. The analytical methodology for the determination of trace pharmaceuticals in complex environmental matrices is still evolving, and the number of methods described in the literature has grown considerably. This volume leads the way, keeping chemistry students, toxicologists, engineers, wastewater managers and related professionals current with developments in this quickly evolving area.
Covers the latest developments in trace determinationsConcise and critical compilation of the recent literature Focuses on new treatment technologies