This book focuses on corrosion and microbial corrosion, providing solutions for these problems based on nanotechnology and nanobiotechnology. It introduces the causes, consequences, cost and control of corrosion processes. It gives a particular emphasis on microbial corrosion of steel and other metals in oil, gas and shipping industries. The book presents the materials vulnerable to such kind of corrosion, and the use of nanomaterials to control it.

Light Microscopic Analysis of Mitochondrial Heterogeneity in Cell Populations and Within Single Cells, by S. Jakobs, S. Stoldt, and D. Neumann
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Advanced Microscopy of Microbial Cells, by J. A. J. Haagensen, B. Regenberg, and C. Sternberg

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Algebraic and Geometric Understanding of Cells, Epigenetic Inheritance of Phenotypes Between Generations, by K. Yasuda

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Measuring the Mechanical Properties of Single Microbial Cells, by C. R. Thomas, J. D. Stenson, and Z. Zhang
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Single Cell Analytics: Pushing the Limits of the Doable,
by H. Kortmann, L.M. Blank, and A. Schmid

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Cultivation-Independent Assessment of Bacterial Viability, by F. Hammes, M. Berney, and T. Egli
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Resolution of Natural Microbial Community Dynamics by Community Fingerprinting, Flow Cytometry and Trend Interpretation Analysis, by P. Bombach, T. Hubschmann, I. Fetzer, S. Kleinsteuber, R. Geyer, H. Harms, and S. Muller
*Multivariate Data Analysis Methods for the Interpretation of Microbial Flow Cytometric Data, by

H.M. Davey, and C.L. Davey

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From Single Cells to Microbial Population Dynamics:

Modelling in Biotechnology Based on Measurements of Individual Cells, by T. Bley"

Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells. In Microbial Metabolic Engineering: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study metabolic engineering. These include methods and techniques to engineer genes and pathways, use of modern biotechnology tools in microbial metabolic engineering, and examples of metabolic engineering for real world applications such as whole cell biosensors and acetate control in large scale fermentation. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microbial Metabolic Engineering: Methods and Protocols seeks to provide researchers with an overview of key topics on microbial metabolic engineering.

Bacterial polysaccharides represent a diverse range of macromolecules that include peptidoglycan, lipopolysaccharides, capsules, and exopolysaccharides - compounds whose functions range from structural cell-wall components (e.g. peptidoglycan) - and important virulence factors (e.g. Poly-N-acetylglucosamine in S. aureus) to permitting the bacterium to survive in harsh environments (e.g. Pseudomonas aeruginosa in the human lung). Polysaccharide biosynthesis is a tightly-regulated, energy-intensive process. Understanding the subtle interplay between the regulation and energy conservation, the polymer modification and synthesis, and the external ecological functions is a huge area of research. The potential benefits are enormous and should enable, for example, the development of novel antibacterial strategies (e.g. new antibiotics and vaccines) and the commercial exploitation to develop novel applications. In this timely book, experienced and authoritative experts review the most important innovations in the research and biotechnological applications of bacterial polysaccharides. The book takes an interdisciplinary view that examines this fascinating subject area in detail from molecular biology, genome-, transcriptome-, and proteome-wide perspectives, and looks at the ecological aspects and systems biology approaches. It provides a sound basis for future research directions, including high efficiency applications of bacterial polysaccharides in medicine, the food industry, and renewable energy production. Topics include: peptidoglycan, lipopolysaccharide, arabinogalactan, capsule gene expression in Escherichia coli, immune response to polysaccharides, polysaccharides from periodontopathic bacteria, role in dental plaque, biofilms, levan, amylovoran, and much more.

The 21st ESACT conference was held in the beautiful surroundings of the CityWest Hotel resort in Dublin, Ireland. For the first time in ESACT history the number of participants exceeded 900: a sign of the ever increasing importance of this area. The conference commenced on Sunday June 5th with two sets of parallel workshops on the subjects listed below. An additional workshop was held on Monday lunchtime of the conferenceProcess Analytical Technology (PAT), Quality by Design (QbD) and other recent regulatory developments.

2. Innovative media products for the 21st century biopharmaceutical industry.

3. The impact of high titre media feed-streams on monoclonal antibody purification.

4. Advances in genomics and proteomics.

5. Stem Cell Technology: new developments and clinical applications.

Conceived with the aim of sorting fact from fiction over genetically modified (GM) crops, this book brings together the knowledge of 30 specialists in the field of transgenic plants. It covers the generation and detection of these plants as well as the genetic traits conferred on transgenic plants. In addition, the book looks at a wide variety of crops, ornamental plants and tree species that are subject to genetic modifications, assessing the risks involved in genetic modification as well as the potential economic benefits of the technology in specific cases. The book s structure, with fully cross-referenced chapters, gives readers a quick access to specific topics, whether that is comprehensive data on particular species of ornamentals, or coverage of the socioeconomic implications of GM technology. With an increasing demand for bioenergy, and the necessary higher yields relying on wider genetic variation, this book supplies all the technical details required to move forward to a new era in agriculture.

"

Adult Stem Cells, second edition, takes a critical look at issues concerning the developmental or differentiation potential for a variety of tissue types and for specific adult stem cell types. Since the first edition appeared a decade ago, our understanding of adult stem cells, and more specifically tissue-specific adult stem cells, has advanced tremendously. And an increased interest in regenerative medicine and potential stem cell applications has driven a quest for better understanding of stem cell biology. In turn, this has spawned much activity on generation and utilization of more and better reagents to identify and isolate stem cells and stem cell-like subpopulations, and on assays elucidating their developmental or differentiation potential and functional integration with host tissues and organs. In this fully updated new edition, chapters cover topics ranging from signaling pathways maintaining stemness in hematopoietic cells to regeneration after injury and endocrine mechanisms underlying the stem cell theory of aging. Other chapters cover stem cells by organ or system including pituitary, cardiac, epithelial, teeth, lung, ovary, prostate, liver, and many more. Importantly, the authors of the chapters have not only summarized their successes, but have also summarized some of the difficulties that each particular field is still facing with respect to maximizing the utility of stem cells in clinical settings. Collectively, they impart both the excitement and challenges facing stem cell utilization for repair and regeneration making this book essential reading for those involved in stem cell research as well as those involved in clinical assays.

The human ambition to reproduce and improve natural objects and processes has a long history, and ranges from dreams to actual design, from Icarus's wings to modern robotics and bioengineering. This imperative seems to be linked not only to practical utility but also to our deepest psychology. Nevertheless, reproducing something natural is not an easy enterprise, and the actual replication of a natural object or process by means of some technology is impossible. In this book the author uses the term naturoid to designate any real artifact arising from our attempts to reproduce natural instances. He concentrates on activities that involve the reproduction of something existing in nature, and whose reproduction, through construction strategies which differ from natural ones, we consider to be useful, appealing or interesting.

The development of naturoids may be viewed as a distinct class of technological activity, and the concept should be useful for methodological research into establishing the common rules, potentialities and constraints that characterize the human effort to reproduce natural objects. The author shows that a naturoid is always the result of a reduction of the complexity of natural objects, due to an unavoidable multiple selection strategy. Nevertheless, the reproduction process implies that naturoids take on their own new complexity, resulting in a transfiguration of the natural exemplars and their performances, and leading to a true innovation explosion. While the core performances of contemporary naturoids improve, paradoxically the more a naturoid develops the further it moves away from its natural counterpart. Therefore, naturoids will more and more affect our relationships with advanced technologies and with nature, but in ways quite beyond our predictive capabilities.

The book will be of interest to design scholars and researchers of technology, cultural studies, anthropology and the sociology of science and technology."

Systems biology is changing the way biological systems are studied by allowing us to examine the cell and organism as a whole. Systems biotechnology allows optimal design and development of upstream to downstream bioprocesses by taking a systems-approach. E. coli has been a model organism for almost all biological and biotechnological studies. This book brings together for the first time the state-of-the-art reviews by the world-leading experts on systems biology and biotechnological applications of E. coli. The topics covered include genomics and functional genomics, resources for systems biology, network analysis, genome-scale metabolic reconstruction, modelling and simulation, dynamic modelling and simulation, systems-level analysis of evolution, plasmids and expression systems, protein synthesis, production and export, engineering the central metabolism, synthetic biology, and systems metabolic engineering of E. coli. This book provides readers with guidance on how a complex biological system can be studied using E. coli as a model organism. It also presents how to perform synthetic biology and systems metabolic engineering studies on E. coli with successful examples, the approaches of which can be extended to other organisms. This book will be a complete resource for anyone interested in systems biology and biotechnology.

Discover the latest, fast-developing technology to help move towards more cost-effective, small-batch, decentralized manufacturing of personalized systems 3D printing has revolutionized manufacturing. Its precision and flexibility have enabled the large-scale production of materials and devices too complex for conventional industrial manufacturing. This has been particularly revolutionary in the field of pharmaceutical production, where 3D printing is being integrated into the manufacture of both drugs and drug delivery devices. It has never been more important for industry professionals to understand this form of production. 3D Printing of Pharmaceuticals and Drug Delivery Devices: Progress from Bench to Bedside offers a comprehensive overview of 3D printing technology and its pharmaceutical applications. It introduces readers to a world in which bespoke drug delivery systems developed for specific users or conditions is rapidly becoming a reality. Its detailed coverage of strategies and industrial processes incorporates the latest research and real-world experience of production. 3D Printing of Pharmaceuticals and Drug Delivery Devices: Progress from Bench to Bedside readers will also find: A multi-disciplinary authorial team of industry leaders Discussion of common technical and regulatory barriers and their possible solutions Far-ranging discussion of pharmaceutical applications across all sectors 3D Printing of Pharmaceuticals and Drug Delivery Devices: Progress from Bench to Bedside is essential reading for pharmaceutical industry professionals and researchers looking to occupy the leading edge.

This book presents an overview of fundamental aspects of surface-based biosensors and techniques for enhancing their detection sensitivity and speed. It focuses on rapid detection using miniaturized sensors and describes the physical principles of nanoscale transducers, surface modifications, microfluidics and reaction engineering, diffusion and kinetics. A key challenge in the field of bioanalytical sensors is the rapid delivery of target biomolecules to the sensing surface. While various nanostructures have shown great promise in sensitive detection, diffusion-limited binding of analyte molecules remains a fundamental problem. Recently, many researchers have put forward novel schemes to overcome this challenge, such as nanopore channels, electrokinetics, and dielectrophoresis, to name but a few. This book provides the readers an up-to-date account on these technological advances.

The book covers the fundamentals of the field of biocatalysis that are not treated in such detail (or even not at all) in existing biocatalysis books or biochemistry textbooks. It of course does not substitute existing biochemistry textbooks but will serve a suitable supplement as it discusses biochemical fundamentals in connection with the respective topics. With focus on the interdisciplinary nature of biocatalysis, the book contains many aspects of fundamental organic chemistry and some of inorganic chemistry as well, which should make it interesting not only for biochemistry but also for chemistry students. An important theme being emphasized in the book is that applied biocatalysis is one of the main prerequisites for a sustainable development. The topics covered ranges from basic enzyme chemistry (biosynthesis, structure, properties, interaction forces, kinetics) to a detailed description of catalytic mechanisms. It covers the fundamentals of the different enzyme classes together with their applications in native and in immobilized state or in the form of whole cells in aqueous as well as non-conventional media. Topics such as catalytic antibodies, nucleic acid catalysts, non-ribosomal peptide synthesis, evolutionary methods, and the design of cells are also included.

The book covers the fundamentals of the field of biocatalysis that are not treated in such detail (or even not at all) in existing biocatalysis books or biochemistry textbooks. It of course does not substitute existing biochemistry textbooks but will serve a suitable supplement as it discusses biochemical fundamentals in connection with the respective topics. With focus on the interdisciplinary nature of biocatalysis, the book contains many aspects of fundamental organic chemistry and some of inorganic chemistry as well, which should make it interesting not only for biochemistry but also for chemistry students. An important theme being emphasized in the book is that applied biocatalysis is one of the main prerequisites for a sustainable development. The topics covered ranges from basic enzyme chemistry (biosynthesis, structure, properties, interaction forces, kinetics) to a detailed description of catalytic mechanisms. It covers the fundamentals of the different enzyme classes together with their applications in native and in immobilized state or in the form of whole cells in aqueous as well as non-conventional media. Topics such as catalytic antibodies, nucleic acid catalysts, non-ribosomal peptide synthesis, evolutionary methods, and the design of cells are also included.

Cellulolytic Enzyme Production and Enzymatic Hydrolysis for Second-Generation Bioethanol Production, by Mingyu Wang, Zhonghai Li, Xu Fang, Lushan Wang und Yinbo Qu Bioethanol from Lignocellulosic Biomass, by Xin-Qing Zhao, Li-Han Zi, Feng-Wu Bai, Hai-Long Lin, Xiao-Ming Hao, Guo-Jun Yue und Nancy W. Y. Ho Biodiesel From Conventional Feedstocks, by Wei Du und De-Hua Liu Establishing Oleaginous Microalgae Research Models for Consolidated Bioprocessing of Solar Energy, by Dongmei Wang, Yandu Lu, He Huang und Jian Xu Biobutanol, by Hongjun Dong, Wenwen Tao, Zongjie Dai, Liejian Yang, Fuyu Gong, Yanping Zhang und Yin Li Branched-Chain Higher Alcohols, by Bao-Wei Wang, Ai-Qin Shi, Ran Tu, Xue-Li Zhang, Qin-Hong Wang und Feng-Wu Bai Advances in Biogas Technology, by Ai-Jie Wang, Wen-Wei Li und Han-Qing Yu Biohydrogen Production from Anaerobic Fermentation, by Ai-Jie Wang, Guang-Li Cao und Wen-Zong Liu Microbial Fuel Cells in Power Generation and Extended Applications, by Wen-Wei Li and Guo-Ping Sheng Fuels and Chemicals from Hemicellulose Sugars, by Xiao-Jun Ji, He Huang, Zhi-Kui Nie, Liang Qu, Qing Xu and George T. Tsao

This book presents the Proceedings of ICON-2019, an international meeting exclusively dedicated to nanostructured materials in medicinal applications. The conference emphasized the recent advances in multidisciplinary research on processing, morphology, structure and properties of nanostructured materials and their applications in various medicinal fields. The papers encompass basic studies and applications and address topics of novel issues, difficulties, and breakthroughs in the field of nanomedicine in cancer, tuberculosis, tissue engineering, regenerative medicine etc.

This book focuses on biogas production by anaerobic digestion, which is the most popular bioenergy technology of today. Using anaerobic digestion for the production of biogas is a sustainable approach that simultaneously also allows the treatment of organic waste. The energy contained in the substrate is released in the form of biogas, which can be employed as a renewable fuel in diverse industrial sectors. Although biogas generation is considered an established process, it continues to evolve, e.g. by incorporating modifications and improvements to increase its efficiency and its downstream applications. The chapters of this book review the progress made related to feedstock, system configuration and operational conditions. It also addresses microbial pathways utilized, as well as storage, transportation and usage of biogas. This book is an up-to-date resource for scientists and students working on improving biogas production.

Describing advanced detectors and their visualization and investigation techniques, this book presents the major applications in nuclear and high-energy physics, astrophysics, medicine and radiation measurements.

This is the fourth updated and revised edition of a well-received book that emphasises on fungal diversity, plant productivity and sustainability. It contains new chapters written by leading experts in the field. This book is an up-to-date overview of current progress in mycorrhiza and association with plant productivity and environmental sustainability. The result is a must hands-on guide, ideally suited for agri-biotechnology, soil biology, fungal biology including mycorrhiza and stress management, academia and researchers. The topic of this book is particularly relevant to researchers involved in mycorrhiza, especially to food security and environmental protection. Mycorrhizas are symbioses between fungi and the roots of higher plants. As more than 90% of all known species of plants have the potential to form mycorrhizal associations, the productivity and species composition and the diversity of natural ecosystems are frequently dependent upon the pre sence and activity of mycorrhizas. The biotechnological application of mycorrhizas is expected to promote the production of food while maintaining ecologically and economically sustainable production systems.

This book addresses the analysis, in the continuum regime, of biological systems at various scales, from the cellular level to the industrial one. It presents both fundamental conservation principles (mass, charge, momentum and energy) and relevant fluxes resulting from appropriate driving forces, which are important for the analysis, design and operation of biological systems. It includes the concept of charge conservation, an important principle for biological systems that is not explicitly covered in any other book of this kind. The book is organized in five parts: mass conservation; charge conservation; momentum conservation; energy conservation and multiple conservations simultaneously applied. All mathematical aspects are presented step by step, allowing any reader with a basic mathematical background (calculus, differential equations, linear algebra, etc.) to follow the text with ease. The book promotes an intuitive understanding of all the relevant principles and in so doing facilitates their application to practical issues related to design and operation of biological systems. Intended as a self-contained textbook for students in biotechnology and in industrial, chemical and biomedical engineering, this book will also represent a useful reference guide for professionals working in the above-mentioned fields.