Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering.

This six volume book set examines a range of topics and applications related to biotechnology. Volumes include fermentation and algal biotechnologies; agricultural biotechnology; medical biotechnology, biopharmaceutics; biosafety, bioethics, biotechnology policy; microbiomes; bioenergy and environmental biotechnology for sustainable development. The topics address significant aspects of the dairy and fermented foods; crop genetics, breeding and genomics; microalgae and novel products; molecular diagnostics and DNA forensics; biosafety, bioethics and legal issues in biotechnology; biotechnology policy advocacy, enlightenment and engagement with stakeholders; medical and pharmaceutical biotechnology; omics and bioinformatics; waste recycling, biofuels and environmental remediation; animal genetics, breeding and genomics; genetic resources conservation and utilization; medicinal and underutilized plants; medical insect biotechnology, genomics and molecular genetics of pests; microbiomes and microbial biotechnology; biotechnology education and curriculum development; and water and waste water research. Volumes include: I: Fermentation and Algal Biotechnologies for the Food, Beverage and other Bioproduct Industries II: Agricultural Biotechnology, Biodiversity and Bio-resources Conservation and Utilization III: Medical Biotechnology, Biopharmaceutics, Forensic Science and Bioinformatics IV: Biosafety and Bioethics in Biotechnology: Policy, Advocacy, and Capacity Building V: Microbiomes and Emerging Applications VI: Bioenergy and Environmental Biotechnology for Sustainable Development The book is a timely knowledge product that documents key issues on advances in biotechnology for use by a variety of readers including postgraduate students, professionals in the field, policy makers, science advocacy groups.

In this 2nd edition of "Micro-Drops and Digital Microfluidics," Jean Berthier explores the fundamentals and applications of digital microfluidics, enabling engineers and scientists to design this important enabling technology into devices and harness the considerable potential of digital microfluidics in testing and data collection.

This book describes the most recent developments in digital microfluidics, with a specific focus on the computational, theoretical and experimental study of microdrops.

Unique in its emphasis on digital microfluidics and with diverse applications ranging from drug delivery to point-of-care diagnostic chips, organic synthesis to microreactors, "Micro-Drops and Digital Microfluidics" meets the needs of audiences across the fields of bioengineering and biotechnology, and electrical and chemical engineering.
Authoritative reporting on the latest changes in microfluidic science, where microscopic liquid volumes are handled as ""microdrops"" and separately from ""nanodrops.""
A methodical examination of how liquid microdrops behave in the complex geometries of modern miniaturized systems and interact with different morphological (micro-fabricated, textured) solid substrates.
A thorough explanation of how capillary forces act on liquid interfaces in contact with micro-fabricated surfaces.
Analysis of how droplets can be manipulated, handled, or transported using electric fields (electrowetting), acoustic actuation (surface acoustic waves), or by a carrier liquid (microflow).
A fresh perspective on the future of microfluidics."

This book is intended to give technological background and practical examples, but also to give general insight into the on-going technology development in the area of biodetection. The content is therefore suitable for an array of stakeholders (decision makers, purchasing officers, etc.) and end-users of biodetection equipment within the areas of health, environment, safety and security, and military preparation. The book is divided into three sections. The first section discusses the fundamental physical and biological properties of bioaerosol's. The second section goes into more detail and discusses in-depth the most commonly used detection principles. The third section of the book is devoted to technologies that have been used in standoff applications. The last section of the book gives an overview of trends in bioaerosol detection. The reader of this book will gain knowledge about the different biodetection technologies and thus better judge their capabilities in relation to desired applications.

Synthetic Biology - A Primer gives a broad overview of the emerging field of synthetic biology and the foundational concepts on which it is built. It will be of interest to final year undergraduates, postgraduates and established researchers who are interested in learning about this exciting new field. The book introduces readers to fundamental concepts in molecular biology and engineering and then explores the two major themes for synthetic biology, namely 'bottom-up' and 'top-down' engineering approaches. 'Top-down' engineering utilises a conceptual framework of engineering and systematic design to build new biological systems by integrating robustly characterised biological parts into an existing system through the use of extensive mathematical modelling. The 'bottom-up' approach involves the design and building of synthetic protocells using basic chemical and biochemical building blocks from scratch. Exemplars of cutting-edge applications designed using synthetic biology principles are presented, including the production of novel biofuels from renewable feedstocks, microbial synthesis of pharmaceuticals and fine chemicals, and the design and implementation of biosensors to detect infections and environmental waste. The book also uses the Internationally Genetically Engineered Machine (iGEM) competition to illustrate the power of synthetic biology as an innovative research and training science. Finally, the primer includes a chapter on the ethical, legal and societal issues surrounding synthetic biology, illustrating the integration of social sciences in synthetic biology research.

The application of methodological approaches and mathematical formalisms proper to Physics and Engineering to investigate and describe biological processes and design biological structures has led to the development of many disciplines in the context of computational biology and biotechnology. The best known applicative domain is tissue engineering and its branches. Recent domains of interest are in the field of biophysics, e.g.: multiscale mechanics of biological membranes and films and filaments; multiscale mechanics of adhesion; biomolecular motors and force generation.

Modern hypotheses, models, and tools are currently emerging and resulting from the convergence of the methods and phylosophycal apporaches of the different research areas and disciplines. All these emerging approaches share the purpose of disentangling the complexity of organisms, tissues, and cells and mimiking the function of living systems.

The contributions presented in this book are current research highlights of six challenging and representative applicative domains of phyisical, engineering, and computational approaches in medicine and biology, i.e tissue engineering, modelling of molecular structures, cell mechanics and cell adhesion processes, cancer physics, and physico-chemical processes of metabolic interactions. Each chapter presents a compendium or a review of the original results achieved by authors in the last years. Furthermore, the book also wants to pinpoint the questions that are still open and that could propel the future research.

This book presents new food production systems (for plants and animals) involving agrochemicals that increase in a controlled manner the bioactives content, under greenhouse conditions. Moreover, conception and design of new instrumentation for precision agriculture and aquiculture contributing in food production is also highlighted in this book.

Volume II presents the latest advances in catalytic hydrodeoxygenation and other transformations of some cellulosic platform chemicals to high value-added products. It presents the theoretical evaluation of the energetics and catalytic species involved in potential pathways of catalyzed carbohydrate conversion, pathways leading to the formation of humin-based by-products, and thermal pathways in deriving chemicals from lignin pyrolysis and hydrodeoxygenation. Catalytic gasification of biomass under extreme thermal conditions as an extension of pyrolysis is also discussed. Marcel Schlaf, PhD, is a Professor at the Department of Chemistry, University of Guelph, Canada. Z. Conrad Zhang, PhD, is a Professor at the Dalian Institute of Chemical Physics, Chinese Academy of Sciences, China.

Reviews in Fluorescence 2016, the tenth volume of the book serial from Springer, serves as a comprehensive collection of current trends and emerging hot topics in the field of fluorescence and closely related disciplines. It summarizes the year's progress in fluorescence and its applications, with authoritative reviews specialized enough to be attractive to professional researchers, yet also appealing to the wider audience of scientists in related disciplines of fluorescence. Reviews in Fluorescence offers an essential reference material for any research lab or company working in the fluorescence field and related areas. All academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in the continuously emerging field of fluorescence will find it an invaluable resource.

The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry. Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system. Written in the highly successful Methods in Molecular Biology (TM) series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Plant molecular biology came to the fore in the early 1980s and there has been tremendous growth in the subject since then. The study of plant genes and genomes and the development of techniques for the incorporation of novel or modified genes into plants eventually led to the commercialisation of genetically modified (GM) crops in the mid-1990s. This was seen as the start of a biotechnological revolution in plant breeding. However, plant biotechnology has become one of the hottest debates of the age and, in Europe at least, one of the greatest challenges that plant scientists have ever faced.This book covers the history and development of the science and techniques that underpin plant biotechnology. It describes the GM crops that are or have been grown commercially around the world, including failures as well as successes, and the new varieties that are being developed. The safety record of GM crops is reviewed together with the legislation that has been adopted to cover their use. The book also deals with the concerns of consumers, the GM crop debate and the prospects for the technology. In the second edition, sections on current GM crops and future developments in plant biotechnology have been greatly expanded, while those on techniques, legislation and the GM crop debate have also been updated.The book is a concise, comprehensive and readable study that is accessible to a general readership with a scientific background but also provides useful information for the specialist.

The series Topics in Current Chemistry Collections presents critical reviews from the journal Topics in Current Chemistry organized in topical volumes. The scope of coverage is all areas of chemical science including the interfaces with related disciplines such as biology, medicine and materials science. The goal of each thematic volume is to give the non-specialist reader, whether in academia or industry, a comprehensive insight into an area where new research is emerging which is of interest to a larger scientific audience. Each review within the volume critically surveys one aspect of that topic and places it within the context of the volume as a whole. The most significant developments of the last 5 to 10 years are presented using selected examples to illustrate the principles discussed. The coverage is not intended to be an exhaustive summary of the field or include large quantities of data, but should rather be conceptual, concentrating on the methodological thinking that will allow the non-specialist reader to understand the information presented. Contributions also offer an outlook on potential future developments in the field. Chapters "Sonocatalysis: A Potential Sustainable Pathway for the Valorization of Lignocellulosic Biomass and Derivatives", "Valorisation of Biowastes for the Production of Green Materials Using Chemical Methods" and "Green and Sustainable Separation of Natural Products from Agro-Industrial Waste: Challenges, Potentialities, and Perspectives on Emerging Approaches" are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

This book review series presents current trends in modern biotechnology. The aim is to cover all aspects of this interdisciplinary technology where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science. Volumes are organized topically and provide a comprehensive discussion of developments in the respective field over the past 3-5 years. The series also discusses new discoveries and applications. Special volumes are dedicated to selected topics which focus on new biotechnological products and new processes for their synthesis and purification. In general, special volumes are edited by well-known guest editors. The series editor and publisher will however always be pleased to receive suggestions and supplementary information. Manuscripts are accepted in English.

Due to various special physiological features and a genome that greatly differs in structure, gene content and organization from other yeasts, Y. lipolytica is widely used as a host system. With its characteristics, such as the ability to grow on lipids or grease, to accumulate oil and the high capacity for secretion of proteases and lipases, the yeast is of great interest for biotechnological applications. The main topics covered in this Microbiology Monograph are: expression and secretion of heterologous proteins; acid and alkaline extracellular proteases; genetics, production, biochemical characterization and biotechnological application of lipases; production and secretion of several organic acids and flagrances; as well as the functional expression of P450 systems and its use in steroid biotransformation.

This textbook is written as a unified approach to various topics, ranging from drug discovery to manufacturing, techniques and technology, regulation and marketing. The key theme of the book is pharmaceuticals - what every student of pharmaceutical sciences should know: from the active pharmaceutical ingredients to the preparation of various dosage forms along with the relevant chemistry, this book makes pharmaceuticals relevant to undergraduate students of pharmacy and pharmaceutical sciences.
This book explains how a particular drug was discovered and then converted from lab-scale to manufacturing scale, to the market. It explains the motivation for drug discovery, the reaction chemistry involved, experimental difficulties, various dosage forms and the reasoning behind them, mechanism of action, quality assurance and role of regulatory agencies. After having a course based on this book, the student will be able to understand: 1) the career prospects in the pharmaceutical industry, 2) the need for interdisciplinary teamwork in science, 3) the techniques and technology involved in making pharmaceuticals starting from bulk drugs, and 4) different dosage forms and critical factors in the development of pharmaceutical formulations in relation to the principles of chemistry.
A few blockbuster drugs including atorvastatin, sildanefil, ranitidine, ciprofloxacin, amoxicillin, and the longest serving drugs such as aspirin and paracetamol are discussed in detail. Finally, the book also covers the important current pharmaceutical issues like quality control, safety, counterfeiting and abuse of drugs, and future prospects for pharmaceutical industry.
Unified approach explaining drug discovery, bulk drug manufacturing, formulation of dosage forms, with pharmacological and therapeutic actionsManufacturing processes of representative active pharmaceutical ingredients and their chemistry plus formulation of dosage forms presented in this book are based on actual industrial processesCovers many aspects relevant to students of the pharmaceutical sciences or newly employed pharmaceutical researchers/employees. It contains summary information about regulatory agencies of different countries

This book highlights the advances in essential oil research, from the plant physiology perspective to large-scale production, including bioanalytical methods and industrial applications. The book is divided into 4 sections. The first one is focused on essential oil composition and why plants produce these compounds that have been used by humans since ancient times. Part 2 presents an update on the use of essential oils in various areas, including food and pharma industries as well as agriculture. In part 3 readers will find new trends in bioanalytical methods. Lastly, part 4 presents a number of approaches to increase essential oil production, such as in vitro and hairy root culture, metabolic engineering and biotechnology. Altogether, this volume offers a comprehensive look at what researchers have been doing over the last years to better understand these compounds and how to explore them for the benefit of the society.

Commercially, D-xylitol is produced by chemical reactions that are tailored to the requirements of various sectors. However, due to the rising interest in sustainable development and ecologically benign practices, microbial transformation processes are generally preferred over the conventional chemical conversion process. The former have multiple advantages, including less chemical load on the environment, higher efficiency, and the ability to dilute multiple downstream transformation attempts while maintaining product yield and recovery.

This book aims to disseminate the most current advances in the biotechnological production of D-xylitol and its applications in medical and health care. It is a unique collection of 15 book chapters split into 5 sections and written by experts in their respective fields, who present critical insights into several topics, review current research, and discuss future progress in this area. This book also provides essential information on hemicellulose hydrolysis to recover D-xylose, detoxification of hemicellulose hydrolysates, and improved fermentation methods for increased D-xylitol production. The highlights of strain improvement to increase the D-xylitol titers and downstream recovery of D-xylitol are also discussed in several sections. The current applications of D-xylitol in medical and health care have been used to justify the cost incurred for setting up the demonstration plant for D-xylitol production in the market. Apart from researchers and post-graduate students in the field of microbial biotechnology, this book will assist those in the business community who deal with the economic analysis of bio-based products and their marketing.

Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation.

Encompasses a variety of different approaches, methodologies and disciplines.

Split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation.