Somatic genome manipulation is required when a sexual crossing approach cannot be used in breeding or genetic treatment of an individual organism. Examples can include gene- or cell-therapy of a person to correct disease, genetic improvement of vegetatively propagated plants, and genetic replacement of cytoplasm without significantly modifying the nuclear genome. The advantage of somatic genome manipulation is maintenance of the general genotype while correcting one or more traits. Somatic genome manipulation is also an option for genetic improvement of sexually propagated plants in polyploidy breeding or in overcoming issues of sexual incompatibility. Recent novel technologies in somatic genome manipulation are developing quickly but much of this literature is fragmented and difficult or inconvenient to access. This book represents the first attempt to assemble updated reviews, detailed protocols, and their applications in all fields in which somatic genome manipulation has thrived. This is a truely one-of-a-kind work that brings together the most important and relevant advances in somatic genome manipulation in plants, algae, microorganisms, humans and animals, and demonstrates where the science interacts and where it diverges. The chapters are written by experts on the topic with ready-to-use protocols that were originally developed or adapted from the literature in their laboratories. We expect that this book will be useful for students, researchers, and teachers in both plant and animal research as a resource for the latest information on somatic genome manipulation and for its useful laboratory methods.

Biological literature of the Roman imperial period remains somehow 'underestimated'. It is even quite difficult to speak of biological literature for this period at all: biology (apart from medicine) did not represent, indeed, a specific 'subgenre' of scientific literature. Nevertheless, writings as disparate as Philo of Alexandria's Alexander, Plutarch's De sollertia animalium or Bruta ratione uti, Aelian's De Natura Animalium, Oppian's Halieutika, Pseudo-Oppian's Kynegetika, and Basil of Caeserea's Homilies on the Creation engage with zoological, anatomic, or botanical questions. Poikile Physis examines how such writings appropriate, adapt, classify, re-elaborate and present biological knowledge which originated within the previous, mainly Aristotelian, tradition. It offers a holistic approach to these works by considering their reception of scientific material, their literary as well as rhetorical aspects, and their interaction with different socio-cultural conditions. The result of an interdisciplinary discussion among scholars of Greek studies, philosophy and history of science, the volume provides an initial analysis of forms and functions of biological literature in the imperial period.

Despite the recent advances made in the improvement of crucifer crops using conventional breeding techniques, the yield levels and the oil and meal quality could not be improved as expected. The understanding of genetic material (DNA/RNA) and its manipulation by scientists has provided the opportunity to improve crucifers by increasing its diversity beyond conventional genetic limitations. The application of the biotechnological techniques will have major impacts in two ways: first, it provides a number of techniques/methods for efficient selection for favorable variants and second, it gives an opportunity to utilize alien variation available in the crucifers by using the novel techniques of biotechnology to develop high yielding varieties with good nutritional quality, having resistance to insect, pest, and disease resistance.

The book deals essentially with the aspects that are of immediate concern to new researchers in the field of botanicals and natural products. It presents the first comprehensive overview of the plant products since they were introduced in the pest management covering both theoretical and practical applications. This book covers the key aspects of the plant products including: Natural pest management agents from plants, extraction of plants products, characterization and formulation and bioassay of extracts against different pests. The book reports for the first time in the field of botanicals, a study on the stability of the prepared extracts towards their various biological activity against different microbial and stored grain pests through a large number of the prepared extracts and formulations in both water and organic media. The book is an indispensable and interdisciplinary text for researchers and scientists from Chemical Sciences, Life Sciences, Agricultural Sciences and related disciplines, working in this important and fascinating area of botanicals and natural products in Integrated Pest Management IPM concept.