This book provides a detailed survey of the more powerful and less conventional HPLC detection methods available today. Addressing both theory and application, experts give their assessment of some of the most promising detection methods being developed. Long lived luminescence, near-infrared semiconductor laser fluorescence, and HPLC-mass spectrometry are among the methods discussed. In addition, an entire chapter is devoted to showing how the ultimate power of NMR may be combined with HPLC.
This book serves the needs of chromatographers who find their work restricted by the limits of conventional HPLC detection methods, and whose practical needs dictate the use of newer, more powerful methods.

Sales Arguments
1. New applications of a very important method are described. 2. Practical aspects are stressed.
3. Inclusion of chapters on photochemical reaction detection makes the book unique and current.
4. Books on chemiluminescence published are orientated toward applications involving enzymatic analysis and immunoassay only.
5. This bookss main target group are applied chromatographers.

Brings together practical information previously available only in numerous separate sources. "Handbook of Analytical Derivation Reactions" begins with an overview of analytical derivatization with a section on apparatus. Describes analytical derivatization methods organized according to the sample to be derivatized. Discussion of methods includes derivative type, reagent, sample type, reaction, and typical procedure with comments and references for each.

"Instrumental Thin-Layer Chromatography" delivers comprehensive coverage of this separation tool with particular emphasis on how this tool can be used in advanced laboratories and integrated into problem-solving scenarios. Significant improvements in instrumentation have outpaced the development of information resources that describe the latest state-of-the-art and demonstrate the full capabilities of TLC. This book provides a contemporary picture of the fundamentals and practical applications of TLC at a level suitable for the needs of professional scientists with interests in project management where TLC is a common tool. Compact, highly focused chapters convey essential information that defines modern TLC and how it can be effectively implemented in most areas of laboratory science. Numerous figures and tables provide access to material not normally found in a single source yet are required by working scientists.
Contributions written by recognized authoritative and visionary expertsFocuses on state-of-the-art instrumental thin-layer chromatography and advanced applications across many areasProvides guidance on the analysis of complex, dirty mixtures of compoundsOffers a cost-effective analytic technique for laboratories working under strict budgets

The book is about the technology and application of Mixed-mode chromatography (MMC). Unlike conventional single-mode HPLC, which resolves the analytes primarily based on their ionic or hydrophobic properties, MMC employs multifunctional stationary phases to exploit at least two modes of interactions (i.e., ionic and hydrophobic) with the analytes and as such often provides resolution that far exceeds that observed with a single-mode process. Over the past two decades, MMC has developed into an important analytical and purification tool in a number of applications in pharmaceutical and biotechnology industries. The technique has been used widely for the analyses of nucleic acids, amino acids, peptides, proteins, glycoproteins, carbohydrates, antibiotics, vaccines, and other products. The purpose of this book is to present a comprehensive survey of mixed-mode chromatography and is intended as a reference guide for graduate students and experienced scientists in pharmaceutical and biotechnology disciplines wishing to gain a deep understanding of this continuously evolving technology.

The rapid development of HPLC instrumentation and technology opens numerous possibilities - and entails new questions. Which column should I choose to obtain best results, which gradient fits to my analytical problem, what are recent and promising trends in detection techniques, what is state of the art regarding LC-MS coupling? All these questions are answered by experts in ten self-contained chapters. Besides these more hardware-related and technical chapters, further related areas of interest are covered: Comparison of recent chromatographic data systems and integration strategies, smart documentation, efficient information search in internet, and tips for a successful FDA inspection. This practical approach offers in a condensed manner recent trends and hints, and will also display the advanced reader mistakes and errors he was not aware of so far.

Principles and Practice of Modern Chromatographic Methods, Second Edition takes a comprehensive, unified approach in its presentation of chromatographic techniques. Like the first edition, the book provides a scientifically rigid, but easy-to-follow presentation of chromatography concepts that begins with the purpose and intent of chromatographic theory - the "what and why" that are left out of other books attempting to cover these principles. This fully revised second edition brings the content up-to-date, covering recent developments in several new sections and an additional chapter on composite methods. New topics include sample profiling, sample preparation, sustainable green chemistry, 2D chromatography, miniaturization/nano-LC, HILIC, and more.

The quantity and composition of aroma and avour compounds in foods and food products exert a marked in uence on the consumer acceptance and, consequently, on the commercial value of the products. It has been established many times that one of the main properties employed for the evaluation of the product quality is the avour, that is, an adequate avour composition considerably enhances the m- ketability. Traditional analytical methods are generally unsuitable for the accurate determination of the quantity of this class of compounds. Moreover, they do not contain any useful information on the concentration of the individual substances and they are not suitable for their identi cation. As the stability of the aroma compounds and fragrances against hydrolysis, oxidation and other environmental and tech- logical conditions shows marked differences, the exact determination of the avour composition of a food or food product may help for the prediction of the she- life of products and the assessment of the in uence of technological steps on the aroma compounds resulting in more consumer-friendly processing methods. Furthermore, the qualitative determination and identi cation of these substances may contribute to the establishment of the provenance of the product facilitating the authenticity test. Because of the considerable commercial importance of avour composition, much effort has been devoted to the development of methods suitable for the separation and quantitative determination of avour compounds and f- grancesinfoodsandinotherindustrialproducts.

Dieses handliche Fachbuch bietet HLPC-Experten ausfuhrliche praktische Informationen, um Gradienten-Trennverfahren zuverlassig und effizient durchzufuhren.

This volume covers various aspects of affinity chromatography along with examples of its applications. Chapters guide readers through methodologies to purify a diverse array of molecular targets such as antibodies, extracellular vesicles, recombinant proteins, biomarkers, metabolites, plant organelles, nucleic acids, ligand identification and protocols on building affinity matrix. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Affinity Chromatography: Methods and Protocols aims to be an invaluable resource to anyone employing affinity chromatography-based methodologies.

High pressure, or high performance, liquid chromatography (HPLC) is the method of choice for checking purity of new drug candidates, monitoring changes during scale up or revision of synthetic procedures, evaluating new formulations, and running control/assurance of the final drug product. HPLC Method Development for Pharmaceuticals provides an extensive overview of modern HPLC method development that addresses these unique concerns. Includes a review and update of the current state of the art and science of HPLC, including theory, modes of HPLC, column chemistry, retention mechanisms, chiral separations, modern instrumentation (including ultrahigh-pressure systems), and sample preparation. Emphasis has been placed on implementation in a pharmaceutical setting and on providing a practical perspective.
HPLC Method Development for Pharmaceuticals is intended to be particularly useful for both novice and experienced HPLC method development chemists in the pharmaceutical industry and for managers who are seeking to update their knowledge.
* Covers the requirements for HPLC in a pharmaceutical setting including strategies for software and hardware validation to allow for use in a regulated laboratory
* Provides an overview of the pharmaceutical development process (clinical phases, chemical and pharmaceutical development activities)
* Discusses how HPLC is used in each phase of pharmaceutical development and how methods are developed to support activities in each phase

The second edition of Fundamentals of Preparative and Nonlinear Chromatography is devoted to the fundamentals of a new process of purification or extraction of chemicals or proteins widely used in the pharmaceutical industry and in preparative chromatography. This process permits the preparation of extremely pure compounds satisfying the requests of the US Food and Drug Administration. The book describes the fundamentals of thermodynamics, mass transfer kinetics, and flow through porous media that are relevant to chromatography. It presents the models used in chromatography and their solutions, discusses the applications made, describes the different processes used, their numerous applications, and the methods of optimization of the experimental conditions of this process.

A unique approach to solving HPLC problems.
Everyone who bought "Problem Solving in HPLC" by Stavros Kromidas will equally benefit from nearly 100 new practical examples for optimization, trouble-shooting, and instrument performance given in this sequel.
The author provides
- guidance for selecting and evaluating methods, intstruments and columns,
- practical help with everyday trouble-shooting,
- advice for optimizing separations, always explaining the reason why.
In each case the problem, the solution and the conclusions are presented over a maximum of 4 pages, and in addition the book contains manufacturers' addresses, references, data tables and checklists.

The third edition of this guidebook provides all the basic applications needed to practice thin layer chromatography (TLC). New material includes the latest techniques on sample preparation and zone detection, the hybridization of TLC with high performance liquid chromatography (HPTLC) as it has been developed in the last few years, emphasis on numerous applications of HPTLC involving pharmaceuticals and drugs, plus the fundamental studies of mechanisms, theories and optimization of TLC.

The book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book's unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here.
Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time.

Updated and expanded, the classic guide to GC/MS helps chromatographers quickly learn to use this technique for analyzing and identifying compounds. After explaining the fundamentals, it discusses optimizing, tuning, using, and maintaining GC/MS equipment; explores advances in miniaturized and field-portable GC/MS systems and microfluidic components; and more. Complete with a CD-ROM, it covers applications in the environmental laboratory and in forensics, toxicology, and space science. This is the premier resource for professionals in those fields and for students.

Recent advances in the pharmaceutical sciences and biotechnology have facilitated the production, design, formulation and use of various types of pharmaceuticals and biopharmaceuticals. This book provides detailed information on the background, basic principles, and components of techniques used for the analysis of pharmaceuticals and biopharmaceuticals. Focusing on those analytical techniques that are most frequently used for pharmaceuticals, it classifies them into three major sections and 19 chapters, each of which discusses a respective technique in detail. Chiefly intended for graduate students in the pharmaceutical sciences, the book will familiarize them with the components, working principles and practical applications of these indispensable analytical techniques.

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.
Key Features
* Liquid chromatography
* Electrophoresis
* Mass spectrometry

This series of books provides coverage of all of the major analytical techniques and their application in the most important areas of physical, life and materials science. Each text is presented in an open learning/distance learning style, in which the learning objectives are clearly identified. The reader's understanding of the material is constantly evaluated by the use of self-assessment and discussion questions.

The combination of gas chromatography with mass spectrometry (GC-MS) has been a routine analytical tool for many years, while the related hybrid technique of liquid chromatography-mass spectrometry (LC-MS) is a rather more recent development. However, with the advent of new interfacing technology, the benefits of this powerful hybrid analytical method are now currently being realised, as witnessed by the exponential growth in applications of this technique in both the chemical and life sciences.

The topics covered in this text include the following:

• The basic principles of liquid chromatography and mass spectrometry

• The limitations of the component techniques when used in isolation, and how a combination of the two allows these to be overcome

• Descriptions of the various approaches, including thermospray, electrospray and atmospheric pressure chemical ionization, which are used to interface the two techniques, along with the advantages and disadvantages of each system

• Illustrative examples of the applications of LC-MS, including the molecular weight and structure determinations of both biopolymers and low-molecular-compounds, along with the development of quantitative methods

Key features include:

• The provision of many discussion and self-assessment questions throughout the text, thus providing opportunities for testing the reader's understanding of the subject matter

• Presented in the AnTS (open learning/distance learning) style, it is ideal for use as both a self-study guide and as the basis of a taught course

• A comprehensive bibliography, including books, papers/articles in journals and on websites, along with specific references for the individual chapters, to guide the reader to more specialised texts and original sources

Written with a student audience in mind, this text will provide an invaluable introduction to this increasingly important analytical tandem technique.

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations.

How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful "HPLC Expert" in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications.

An indispensable resource for busy researchers
Your time is valuable-too valuable to spend hunting through the technical literature in search of the right HPLC assay techniques for your projects. With HPLC Methods for Recently Approved Pharmaceuticals, you'll quickly identify and replicate the ideal procedures for your project needs, without having to refer to original source publications. More of your time can then be spent in the lab, not the library.
Covering the relevant world literature through 2003, this book picks up where Dr. Lunn's acclaimed HPLC Methods for Pharmaceutical Analysis left off. It arms you with established HPLC assay techniques for hundreds of newly approved drugs, as well as drugs for which assay methods were only recently developed. Combining detailed descriptions of procedures with specially annotated references, this practical handbook gives you:
* HPLC methods for 390 commonly prescribed pharmaceutical compounds
* Various procedures for each drug listed together-making it easy to mix and match for customized approaches
* Methods for drugs in biological fluids and for bulk and formulated drugs
* Chemical structures, molecular weights and formulas, and CAS Registry Numbers
* Cross-references to The Merck Index
* Retention times of other drugs that can be assayed using the same methods

Jump into the HPLC adventure

Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners.

New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresisHow to obtain peak capacityvan Deemter curves and other coherencesHydrophilic interaction chromatographyMethod transferComprehensive two-dimensional HPLCFast separations at 1000 barHPLC with superheated water

In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added.

A completely new chapter is presented on quality assurance covering: Is it worth the effort?Verification with a second methodMethod validationStandard operating proceduresMeasurement uncertaintyQualifications, instrument test, and system suitability testThe quest for quality

Reviews of earlier editions

"That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." --"The Analyst"

..".provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories...a valuable introduction." - "American Journal of Therapeutics"

An in-depth guide to HPLC column technology High-performance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries; environmental laboratories; and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now, no book has focused on the theory and practice of column technology. HPLC Columns provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and academician alike. Along with a comprehensive discussion of the chemical and physical processes of the HPLC column, it includes fundamental principles, separation mechanisms and available technologies, column selection criteria, and special techniques. Special features include:

• Comprehensive overview of state-of-the-art HPLC column technology
• Explanation of the underlying principles of HPLC columns
• Methods for selecting columns
• Practical advice on using and applying columns, including examples
• Section by M. Zoubair El Fallah on methods development
• Special techniques, including preparative chromatography, continuous chromatography, and the simulated moving bed
• Troubleshooting section

HPLC Columns helps laboratory practitioners make better choices in column selection, methods development, and troubleshooting: it is also an excellent textbook for graduate-level courses and HPLC short courses.

Analytical techniques based on separation processes, such as chromatography and electrophoresis, are finding a growing range of applications in chemical, pharmaceutical and clinical laboratories. The Wiley Separation Science Series provides the analyst in these laboratories with well-focused books covering individual techniques, so that they can be applied more efficiently and effectively to contemporary analytical problems. The different enantiomers of a drug can exhibit widely different physiological activity in degree and nature. As a result, the separation and identification of enantiomers is now a very important analytical problem and chiral chromatography is the natural technique to apply to the resolution of such mixtures. Chiral Chromatography provides the reader with a basic understanding of the nature of chromatographic separations and relates the principles specifically to the separation of enantiomers. The following information is included:

• chiral separations involving both gas and liquid chromatography
• descriptions of the apparatus used for both techniques
• detailed discussion on the retention mechanism that results in chiral selectivity
• the structure and synthesis of a wide range of chirally active stationary phases used in both gas and liquid chromatography
• preparative applications for large scale purification of enantiomers
• applications of capillary electrophoresis and capillary electrochromatography.

In addition to the above, a large number of examples of the separation of both commercially and physiologically interesting chiral mixtures are given, as is a detailed discussion on the mechanism of selectivity of each example. Thomas Beesley was founder and is the CEO for a leading manufacturer of chiral stationary phases and has published papers on TLC, HPLC and chiral separations involving cyclodextrins. He has also coauthored papers with Daniel W. Armstrong, an expert on modern cyclodextrin columns. Raymond Scott has worked in the field of separation science for over 40 years and has contributed extensively to the development of both gas and liquid chromatography publishing over 160 papers on the subjects.