The analysis and modification of glycans of recombinant proteins continues to be active and challenging area of research and for the successful manufacture of these proteins. In Cell Engineering, volume 3: Glycosylation, Dr. Mohammed Al-Rubeai has compiled a group of articles that will provide research workers not only with reviews of the advances that have been made in all facets of the subject but with an in-depth assessment of the state of the art methodology and the various approaches for the improvement of glycoprotein production. Particularly important in this respect is the advances made in the development of genetically engineered host cell lines with novel glycosylation properties, as well as the integration of mass spectrophotometric analysis with separation techniques.

This volume is intended not only for research students and senior scientists in cell culture and glycobiology, but also for industrial biotechnologists and biochemical engineers interested in the production of therapeutic glycoproteins, virus vector and ex vivo expansion of human cells for medical treatment.

More then 20 years have passed now since the first recombinant protein producing microorganisms have been developed. In the meanwhile, numerous proteins have been produced in bacteria, yeasts and filamentous fungi, as weIl as higher eukaryotic cells, and even entire plants and animals. Many recombinant proteins are on the market today, and some of them reached substantial market volumes. On the first sight one would expect the technology - including the physiology of the host strains - to be optimised in detail after a 20 year's period of development. However, several constraints have limited the incentive for optimisation, especially in the pharmaceutical industry like the urge to proceed quickly or the requirement to define the production parameters for registration early in the development phase. The additional expenses for registration of a new production strain often prohibits a change to an optimised strain. A continuous optimisation of the entire production process is not feasible for the same reasons.

Enzymatic catalysis has gained considerable attention in recent years as an efficient tool in the preparation of natural products, pharmaceuticals, fine chemicals, and food ingredients. The high selectivity and mild reaction con- tions associated with enzymatic transformations have made this approach an attractive alternative in the synthesis of complex bioactive compounds, which are often difficult to obtain by standard chemical routes. However, the maj- ity of organic compounds are not very soluble in water, which was traditi- ally perceived as the only suitable reaction medium for the application of biocatalysts. The realization that most enzymes can function perfectly well under nearly anhydrous conditions and, in addition, display a number of useful properties, e. g. , highly enhanced stability and different selectivity, has d- matically widened the scope of their application to the organic synthesis. Another great attraction of using organic solvents rather than water as a reaction solvent is the ability to perform synthetic transformations with re- tively inexpensive hydrolytic enzymes. It is worth reminding the reader that in vivo, the synthetic and hydrolytic pathways are catalyzed by different enzymes. However, elimination of water from the reaction mixture enables the "reversal" of hydrolytic enzymes and thus avoids the use of the expensive cofactors or activated substrates that are required for their synthetic count- parts.

The next article includes the description of the rich chemistry of phosphinines, including azaphosphinines. The sixth article deals with synthetic approaches to different types of 1- heterophosphacyclanes, including four-, five-, and six-membered P-heterocycles. The next two articles cover the chemistry of phosphorus containing mac- cycles. The phosphorus containing calixarenes have attracted much attention in recent years due to their various functions such as metal cations binding, catalysis, molecular recogination, and bioactivity. Likewise, other phosphorus-containing macrocycles, cryptands, and dendrimers find various uses in analytical chemistry and biochemistry. We hope to include the following articles in the second volume on phosphorous heterocycles: Diazaphospholes Selected phosphorous heterocycles containing a stereogenic phosphorus Heterophenes carrying phosphorus functional groups as key structures The synthesis and chemistry of the phospholane ring system Synthesis and bioactivity of 2,5-dihydro-1,2-oxaphosphole-2-oxide derivatives Recent developments in the chemistry of N-heterocyclic phosphines. I would be failing in my duty if I do not express my sincere thanks to the people at Springer, particularly Ms. Birgit Kollmar-Thoni and Ms. Ingrid Samide, for coordinating the project with great dedication.

This book starts from the fundamentals to the professional level, academic, practical and industrial classification and understanding of the many types and mechanisms of chemical reactions before illustrating the generalised kinetics and stoichiometry which may be applied to them. Several typical and numerical problems are solved in chemical kinetics, stoichiometry, material and energy balances relevant to the chemical engineering aspects of chemical reactor design.

Concerned with discovering the chemical pathways of biosynthesis, this book devotes four chapters to the use of isotopes in biosynthetic research and the biosynthesis of enzyme cofactors and vitamin B12 and of reduced polyketides such as erythromycin. The topics covered demonstrate the revolution that has occurred in biosynthetic studies with the advent of gene cloning and overexpression. Yet the book also shows that the more classical approach to biosynthetic studies must go hand in hand with these new techniques.

This book covers the material required for a basic understanding of chemical reaction engineering. Such material would normally be taught in a first chemical reaction engineering course in a university chemical engineering department. The principles of reaction engineering are simply and clearly presented; simple illustrative problems are used to demonstrate how these principles are practically applied. Further problems, with solutions, based on exam questions, are supplied. The book is written in a way that it could be used as a self-study guide and would be useful for undergraduate chemical engineers early in their degree as well as engineers and scientists of other disciplines interested in acquiring some knowledge of reaction engineering outside of a formal teaching environment.

This book provides an advanced level introduction to the electrochemistry. It provides the background required to follow current research in a subject which has again become highly topical through the recent development of surface sensitive techniques. It is divided into three parts covering the fundamentals of the subject, the experimental methods including surface sensitive techniques, and a few important problems in theoretical electrochemistry. A special chapter is devoted to liquid-liquid interfaces which have become of interest because of their relation to biological membranes.

This book offers a bridge at the interface between engineering and cell biology, demonstrating how a mathematical modeling approach combined with quantitative experiments can provide enhanced understanding of cell phenomena involving receptor/ligand interactions. Model frameworks are described over the entire spectrum of receptor processes, from fundamental cell surface binding, intracellular trafficking, and signal transduction events to the cell behavioural functions they govern, including proliferation, adhesion, and migration.

From reviews of the hardback:

Itraconazole nanoparticles with suitable size ranges are expected to improve the therapeutic efficacy and reduction of toxicity of this broad spectrum antifungal agent. Components of the SLNs were lipid (palmitic acid) and surfactants (Pluronic F127 and Tween 40). The Itraconazole loaded nanoparticles were prepared by microemulsion dispersion method. Particles size analysis and zeta potential measurements were done using Malvern Mastersizer Hydro 2000G. The particles were also subjected to DSC, IR and XRD analyses.The study elaborates on the feasibility and suitability of lipid based colloidal drug delivery system, employing optimize design to develop a clinically useful nanoparticle system with targeting potential. It is expected that this type of itraconazole loaded lipid nanoparticulate system could be clinically effective in better management of systemic and ocular inflammation with greater degree of safety and efficacy. Therefore, no doubt that the book will be of great use for the researchers those who are working on nano drug delivery system for BCS class drugs in India and abroad as well.

Containing over 1200 detailed equations and illustrations, Biochemical Engineering offers several features that make it an ideal textbook. For students. edifying worked-out examples problems thought-provoking end-of-chapter exercises helpful definitions of nomenclature a useful key word index For instructors.. outlines for an undergraduate 15-week semester course on biochemical engineering for students who have had an introductory class in biochemistry or a related biological science, or who are taking such a course concurrently additional or alternate topics for a graduate course for students without background in biological sciences and a timesaving solutions manual (available to instructors only)

This unique text is the first to demonstrate, to suppliers and users of paste flow equipment, a scientific means of approaching design and operation. It will be indispensable in developing new products and processes in addition to enhancing existing ones. All types of equipment are considered and particular emphasis is given to paste characterization and die design. Based on 25 years' indusrial experience and research work, the text blends theory and practice, with emphasis on the practical applications, for anyone with a background in engineering or science.

Uses a large number of industrially-significant problems to convey an in-depth understanding of modern calculation procedures. Includes numerous topical examples and problems, and both conventional and SI units.

3D industrial printing has become mainstream in manufacturing. This unique book is the first to focus on polymers as the printing material. The scientific literature with respect to 3D printing is collated in this monograph. The book opens with a chapter on foundational issues such and presents a broad overview of 3D printing procedures and the materials used therein. In particular, the methods of 3d printing are discussed and the polymers and composites used for 3d printing are detailed. The book details the main fields of applications areas which include electric and magnetic uses, medical applications, and pharmaceutical applications. Electric and magnetic uses include electronic materials, actuators, piezoelectric materials, antennas, batteries and fuel cells. Medical applications are organ manufacturing, bone repair materials, drug-eluting coronary stents, and dental applications. The pharmaceutical applications are composite tablets, transdermal drug delivery, and patient-specific liquid capsules. A special chapter deals with the growing aircraft and automotive uses for 3D printing, such as with manufacturing of aircraft parts and aircraft cabins. In the field of cars, 3D printing is gaining importance for automotive parts (brake components, drives), for the fabrication of automotive repair systems, and even 3D printed vehicles.

This textbook covers the fundamentals of physical chemistry, explaining the concepts in an accessible way and guiding the readers in a step-by-step manner. The contents are broadly divided into two sections: the classical physico-chemical topics (thermodynamics, kinetics, electrochemistry, transport, and catalysis), and the fabric of matter and its interactions with radiation. Particular care has been taken in the presentation of the algebraic parts of physico-chemical concepts, so that the readers can easily follow the explanations and re-work relevant discussion and derivations with pen and paper. The book is accompanied by a rich mathematical appendix. Each chapter includes a selection of (numerical) exercises and problems, so that students can practice and apply the learned topics. An appendix with solutions allows for controlling the learning success. Carefully prepared illustrative color images make this book a great support for teaching physical chemistry to undergraduate students.This textbook mainly addresses undergraduate students in life sciences, biochemistry or engineering, offering them a comprehensive and comprehensible introduction for their studies of physical chemistry. It will also appeal to undergraduate chemistry students as an accessible introduction for their physical chemistry studies.