The goal of this book is to make some underutilized but potentially very useful methods in experimental design and analysis available to ecologists, and to encourage better use of standard statistical techniques. Ecology has become more and more an experimental science in both basic and applied work, but experiments in the field and in the laboratory often present formidable statistical difficulties. Organized around providing solutions to ecological problems, this book offers ways to improve the statistical aspects of conducting manipulative ecological experiments, from setting them up to interpreting and reporting the results. An abundance of tools, including advanced approaches, are made available to ecologists in step-by-step examples, with computer code provided for common statistical packages. This is an essential how-to guide for the working ecologist and for graduate students preparing for research and teaching careers in the field of ecology.

It is now more than ten years since Dr. Alec Jeffreys (now Professor Sir Alec Jeffreys, FRS) reported in Nature that the investigation of certain minisatellite regions in the human genome could produce what he termed DNA fingerprints and provide useful information in the fields of paternity testing and forensic analysis. Since that time we have witnessed a revolution in the field of forensic identification. A total change of technology, from serological or electrophoretic analysis of protein polymorphisms to direct investigation of the underlying DNA polymorphisms has occurred in a short space of time. In addition, the evolution and development of the DNA systems themselves has been rapid and spectacular. In the last decade we have progressed from the multilocus DNA fing- prints, through single locus systems based on the same Southern blot RFLP technology, to a host of systems based on the PCR technique. These include Allele Specific Oligonucleotide (ASO)-primed systems detected by dot blots, the "binary" genotypes produced by mapping variations within VNTR repeats demonstrated by minisatellite variant repeat (MVR) analysis, and yet other fragment-length polymorphisms in the form of Short Tandem Repeat (STR) loci. Hand in hand with the increasing range of systems available has been the development of new instrumentation to facilitate their analysis and allow us to explore the possibilities of high volume testing in the form of mass scre- ing and offender databases.

Offering all aspects of humidity measurement and instrumentation, this work includes rudiments and theory, common applications, advantages and limitations of frequently-used sensors and techniques, and guidelines for installation, maintenance and calibration. The disk is intended for easy conversions of humidity parameters and units.

Response Surfaces: Designs and Analyses; Second Edition presents techniques for designing experiments that yield adequate and reliable measurements of one or several responses of interest, fitting and testing the suitability of empirical models used for acquiring information from the experiments, and for utilizing the experimental results to make decisions concerning the system under investigation.
This edition contains chapters on response surface models with block effects and on Taguchi's robust parameter design, additional details on transformation of response variable, more material on modified ridge analysis, and new design criteria, including rotatability for multiresponse experiments. It also presents an innovative technique for displaying correlation among several response. Numerical examples throughout the book plus exercises--with worked solutions to selected problems--complement the text.

Let this down-to-earth book be your guide to the statistical integrity of your work. Without relying on the detailed and complex mathematical explanations found in many other statistical texts, Principles of Experimental Design for the Life Sciences teaches how to design, conduct, and interpret top-notch life science studies. Learn about the planning of biomedical studies, the principles of statistical design, sample size estimation, common designs in biological experiments, sequential clinical trials, high dimensional designs and process optimization, and the correspondence between objectives, design, and analysis. Each of these important topics is presented in an understandable and non-technical manner, free of statistical jargon and formulas. Written by a biostatistical consultant with 25 years of experience, Principles of Experimental Design for the Life Sciences is filled with real-life examples from the author's work that you can quickly and easily apply to your own. These examples illustrate the main concepts of experimental design and cover a broad range of application areas in both clinical and nonclinical research. With this one innovative, helpful book you can improve your understanding of statistics, enhance your confidence in your results, and, at long last, shake off those statistical shackles!

This work elucidates the power of modern nuclear magnetic resonance (NMR) techniques to solve a wide range of practical problems that arise in both academic and industrial settings. This edition provides current information regarding the implementation and interpretation of NMR experiments, and contains material on: three- and four-dimensional NMR; the NMR analysis of peptides, proteins, carbohydrates and oligonucleotides; and more.

Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.

Proceedings of the 51st Course of the International School of Subnuclear Physics on 'Reflections on the next step for LHC', Erice, 24 June - 3 July 2013.

The Second Edition of this bestseller brings together basic plant pathology methods published in diverse and often abstract publications. The Second Edition is updated and expanded with numerous new figures, new culture media, and additional methods for working with a greater number of organisms. Methods are easy to use and eliminate the need to seek out original articles. This reference allows for easy identification of methods appropriate for specific problems and facilities. Scientific names of pathogens and some of their hosts are updated in this edition. The book also acts as a research source providing more than 1,800 literature citations.
The Second Edition includes chapters on the following:
Sterilization of culture apparatus and culture media
Culture of pathogens with detailed techniques for 61 fungi and selected bacteria
Long-term storage of plant pathogens
Detection and estimation of inoculum for 28 soilborne fungal pathogens and 5 bacterial genera-15 methods for airborne inoculum and 13 methods for seedborne pathogens
Establishment of disease and testing for disease resistance
Work with soil microorganisms
Fungicide evaluation
Biological control
Bright-field microscopy

In this book, the major paradigm-shifting discoveries made in the past century on key cellular nanomachines are described in great detail: their complex yet precise and elegant design and function, as well as the diseases linked to their dysfunction and the therapeutic approaches to overcome them. The major focus of this book is the "porosome" nanomachine, the universal secretory portal in cells. This is an ideal book for students, researchers, and professionals in the fields of nanoscience and nanotechnology.

Trace Analysis is a highly practical book which deals with the science rather than the paperwork of quality assurance systems. Produced as part of the UK Valid Analytical Measurement (VAM) initiative, it provides the analyst with a systematic approach across the broad spectrum of trace analysis, offering practical advice and guidance on methodology and techniques. The book is structured to take the analyst step-by-step through the stages of any trace analysis. The approach is general, being broken down only into types of analyte. Additional chapters explain the application of groups of techniques to each analyte type. Each section contains references to published material which will allow the analyst to obtain further information on specific topics. Throughout the book, the analyst is reminded of pitfalls which lead to unreliable results. This new book therefore offers invaluable advice to analysts in all areas and at all levels, providing practical 'expert' advice on methodology. It will prove indispensable as a single, comprehensive bench guide for analysts in university, college and industrial laboratories.

We cannot imagine a world without plastics. Plastic products make our daily life safe, healthy and convenient. Besides all the benefits, the current plastics economy gives rise to environmental concerns with respect to fossil oil depletion and plastic waste accumulation. In a circular economy, however, plastics can be redesigned for reusability and recyclability. This book makes the topic of sustainable plastics approachable for students and career starters alike, describing the nature and chemistry of (bio)polymers as well as how to create a closed loop of plastic materials.

This updated and revised edition of a classic work provides a summary of methods for numerical computation of high resolution conventional and scanning transmission electron microscope images. At the limits of resolution, image artifacts due to the instrument and the specimen interaction can complicate image interpretation. Image calculations can help the user to interpret and understand high resolution information in recorded electron micrographs. The book contains expanded sections on aberration correction, including a detailed discussion of higher order (multipole) aberrations and their effect on high resolution imaging, new imaging modes such as ABF (annular bright field), and the latest developments in parallel processing using GPUs (graphic processing units), as well as updated references. Beginning and experienced users at the advanced undergraduate or graduate level will find the book to be a unique and essential guide to the theory and methods of computation in electron microscopy.

A time-tested, systematic approach to the buying and selling of complex research instruments

Searching for the best laboratory instruments and systems can be a daunting and expensive task. A poorly selected instrument can dramatically affect results produced and indirectly affect research papers, the quality of student training, and an investigator's chances for advancement. "Buying and Selling Laboratory Instruments" offers the valuable insights of an analytical chemist and consultant with over four decades of experience in locating instruments based upon both need and price. It helps all decision makers find the best equipment, service, and support while avoiding the brand-loyalty bias of sales representatives so you can fully meet your laboratory's requirements.

The first section of the book guides buyers through the hurdles of funding, purchasing, and acquiring best-fit instruments at the least-expensive price. It explains how to find vendors that support their customers with both knowledgeable service and application support. Also offered is guidance on adapting your existing instruments to new applications, integrating new equipment, and what to do with instruments that can no longer serve in research mode.

The second section explains the sales process in detail. This is provided both as a warning against manipulative sales reps and as a guide to making the sale a win-win process for you and your vendor. It also shows you how to select a knowledgeable technical guru to help determine the exact system configuration you need and where to find the best price for it. Added bonuses are summary figures of buying sequence and sales tools and an appendix containing frequently asked questions and memory aids.

"Buying and Selling Laboratory Instruments" is for people directly involved in selecting and buying instruments for operational laboratories, from the principle investigator to the person actually delegated with investigating and selecting the system to be acquired. Sales representatives; laboratory managers; universities; pharmaceutical, biotech, and forensic research firms; corporate laboratories; graduate and postdoctoral students; and principle investigators will not want to be without this indispensible guide.

Ideal for use with any introductory physics text, Loyd's PHYSICS LABORATORY MANUAL is suitable for either calculus- or algebra/trigonometry-based physics courses. Designed to help students demonstrate a physical principle and learn techniques of careful measurement, Loyd's PHYSICS LABORATORY MANUAL also emphasizes conceptual understanding and includes a thorough discussion of physical theory to help students see the connection between the lab and the lecture. Available with InfoTrac Student Collections http: //gocengage.com/infotrac.

This book provides a comprehensive overview of the latest developments in the field of spin dynamics and magnetic damping. It discusses the various ways to tune damping, specifically, dynamic and static control in a ferromagnetic layer/heavy metal layer. In addition, it addresses all optical detection techniques for the investigation of modulation of damping, for example, the time-resolved magneto-optical Kerr effect technique.

This book is written to help and enable students in how to observe biological specimens in terms of viscosity, mass, elasticity and work producing elements. The observations are related to underlying chemical reactions by means of strain (fractional length change) sensitivity of the reactions, and a theory is developed how to connect these. Their mathematical derivation is complex when three or more states are involved, but a method is presented here to demonstrate how to simplify this complex problem. Basic mathematical solutions that are useful for this book, are presented (Fourier and Laplace transforms, differential equations, matrix operations) together with Fortran programs in the Appendix.

Archaeologists and archaeology students have long since needed an authoritative account of the techniques now available to them, designed to be understood by non-scientists. This book fills the gap and it offers a two-tier approach to the subject. The main text is a coherent introduction to the whole field of science-based dating, written in plain langauge for non-scientists. Additional end-notes, however, offer a a more technical understanding, and cater for those who have a scientific and mathematical background.

This open access book brings out the state of the art on how informatics-based tools are used and expected to be used in nanomaterials research. There has been great progress in the area in which "big-data" generated by experiments or computations are fully utilized to accelerate discovery of new materials, key factors, and design rules. Data-intensive approaches play indispensable roles in advanced materials characterization. "Materials informatics" is the central paradigm in the new trend. "Nanoinformatics" is its essential subset, which focuses on nanostructures of materials such as surfaces, interfaces, dopants, and point defects, playing a critical role in determining materials properties. There have been significant advances in experimental and computational techniques to characterize individual atoms in nanostructures and to gain quantitative information. The collaboration of researchers in materials science and information science is growing actively and is creating a new trend in materials science and engineering.

Since the first recognition of outbreaks of cerebrospinal or spotted fever at the end of the nineteenth and the beginning of the twentieth centuries, the menace of the meningococcus has been high on the list of public health prio- ties. Few if any pathogens surpass the meningococcus in the rapidity and sev- ity with which it devastates previously healthy individuals. The challenge of understanding the biology of this fascinating microbe is immense, but few will doubt that successful control of meningococcal meningitis and septicemia will only transpire through the application of a body of extraordinary detailed inf- mation, including key minutiae of its molecular biology. In the first of two companion volumes, Meningococcal Vaccines, the team of experts gathered by Andy Pollard and Martin Maiden converge to p- vide an impressive accumulation of molecular tools with which to lay bare the secrets of the meningococcus. The results of this ambitious and welcome v- ume represent an exciting and much needed resource for all of us in the field.

This guide covers aspects of designing microarray experiments and analysing the data generated, including information on some of the tools that are available from non--commercial sources. Concepts and principles underpinning gene expression analysis are emphasised and wherever possible, the mathematics has been simplified. The guide is intended for use by graduates and researchers in bioinformatics and the life sciences and is also suitable for statisticians who are interested in the approaches currently used to study gene expression.* Microarrays are an automated way of carrying out thousands of experiments at once, and allows scientists to obtain huge amounts of information very quickly* Short, concise text on this difficult topic area* Clear illustrations throughout* Written by well--known teachers in the subject* Provides insight into how to analyse the data produced from microarrays

This informationpacked book Great Personalities of India chronicles the lives of influential personalities whose achievements have helped shape and reshape the contours of India and its people. The compilation includes outstanding statesmen and women, entrepreneurs, scientists, social reformers, film artists, sportsmen and women, among others. Also included in the book are eminent people who have achieved distinction as writers, musicians, business persons, philanthropists, etc. Enjoy reading live and sacrifice of the 80 famous personalities who have influenced our lives, and are a source of constant inspiration and motivation for all of us. The text is organised categorywise for easy search and reference. Each biography includes life sketch, a trivia, a quote, successes & failures and awards associated with him/her to inspire the readers and satisfy their curious instincts. Open up the pages of the book to read the success stories, and discover for yourself how fast you can imbibe their traits to make it big in your life.

The accurate measurement of temperature is a vital parameter in many fields of engineering and scientific practice. Responding to emerging trends, this classic reference has been fully revised to include coverage of the latest instrumentation and measurement methods.

Featuring:

• Brand new chapters on computerised temperature measuring systems and temperature measurement in medicine.

• Sections on noise thermometers, the development of photoelectric and two-wave pyrometers and the latest IEC (International Electrotechnical Commission) standards

• Coverage of fibre optic thermometers, imaging of temperature fields and measurement in hazardous areas.

• Examination of virtual instruments, fuzzy logic in temperature measurement and new methods for thermometer calibration.

Dealing with the volume, complexity, and diversity of data currently being generated by scientific experiments and simulations often causes scientists to waste productive time. Scientific Data Management: Challenges, Technology, and Deployment describes cutting-edge technologies and solutions for managing and analyzing vast amounts of data, helping scientists focus on their scientific goals. The book begins with coverage of efficient storage systems, discussing how to write and read large volumes of data without slowing the simulation, analysis, or visualization processes. It then focuses on the efficient data movement and management of storage spaces and explores emerging database systems for scientific data. The book also addresses how to best organize data for analysis purposes, how to effectively conduct searches over large datasets, how to successfully automate multistep scientific process workflows, and how to automatically collect metadata and lineage information. This book provides a comprehensive understanding of the latest techniques for managing data during scientific exploration processes, from data generation to data analysis. Enhanced by numerous detailed color images, it includes real-world examples of applications drawn from biology, ecology, geology, climatology, and more. Check out Dr. Shoshani discuss the book during an interview with International Science Grid This Week (iSGTW): http://www.isgtw.org/?pid=1002259

The evolution of observational instruments, simulation techniques, and computing power has given aquatic scientists a new understanding of biological and physical processes that span temporal and spatial scales. This has created a need for a single volume that addresses concepts of scale in a manner that builds bridges between experimentalists and theoreticians in aquatic ecology. Handbook of Scaling Methods in Aquatic Ecology: Measurement, Analysis, Simulation is the first comprehensive compilation of modern scaling methods used in marine and freshwater ecological research. Written by leading researchers, it presents a systematic approach to dealing with space and time in aquatic ecology. This is a compendium that analyzes themes related to the response or behavior of organisms to processes occurring over multiple spatial and temporal scales. This book covers: novel techniques for data collection, focusing on processes over a broad range of scales (from bacteria to ocean basins); newly-developed concepts and data analysis algorithms; and innovative computer models and simulations to mimic complex biological processes. The Handbook serves as a reference volume for investigators seeking insight into new experimental approaches and data analysis, as well as the sensor design required for optimal sampling. Many of the algorithms and models provided are directly applicable to your experimental data. This comprehensive treatment of scaling methods and applications can help foster a unified understanding of subject matter among the modeling, experimental, and field research communities.