This first edited Volume on IgY-Technology, addresses the historical and dynamic development of IgY-applications. The authors cover the biological basis and theoretical context, methodological guidance, and applications of IgY-Technology. A focus is laid on the use of IgY-antibodies for prophylactic/therapeutic purposes in human and veterinary medicine. Aside from applications, the chapters also offer an evolutionary understanding of the IgY molecule, IgY receptors and practical prerequisites to produce IgY-antibodies. Guidance is given for every step of the process. Starting with an introduction to hens as a model species and including hen husbandry, hen egg-laying capacity and total IgY outcomes. Readers will also learn about immunization techniques, the advantages and limitations of different IgY extraction methods, as well as storage stability of the final product. The last part of the volume highlights hands-on aspects of applications, such as IgY delivery strategies, new methods to produce monoclonal IgY-antibodies or production of functional IgY fragments by phage-display as well as commercial exploitation of the technology. Thus, this book is a valuable resource and guide for Scientists, Clinicians and Health Product Developers in both human and veterinary medicine.

This book presents a detailed look at experimental and computational techniques for accurate structure determination of free molecules. The most fundamental property of a molecule is its structure - it is a prerequisite for determining and understanding most other important properties of molecules. The determination of accurate structures is hampered by a myriad of factors, subjecting the collected data to non-negligible systematic errors. This book explains the origin of these errors and how to mitigate and even avoid them altogether. It features a detailed comparison of the different experimental and computation methods, explaining their interplay and the advantages of their combined use. Armed with this information, the reader will be able to choose the appropriate methods to determine - to a great degree of accuracy - the relevant molecular structure.

Analytical techniques are powerful tools in a chemist's armoury. Spectroscopic data and chemical information are used routinely in laboratories to follow a chemical reaction or elucidate a chemical structure. However, the sophistication of the analytical techniques used changes rapidly, hence the routinely used method of today can all too readily be superseded by the new technology of tomorrow. More Modern Chemical Techniques identifies some applications of the important chemical techniques in use today that are less well known in schools and colleges and which illustrate how chemistry is using state-of-the-art technology to push back the frontiers of the subject. Examples include: elemental analysis such as atomic absorption spectrometry and inductively coupled plasma techniques; separations including electrophoresis, structure determination (eg x-ray diffraction and optical microscopy); and sampling and sample preparation.

Introduction to Statistical Analysis of Laboratory Data presents a detailed discussion of important statistical concepts and methods of data presentation and analysis * Provides detailed discussions on statistical applications including a comprehensive package of statistical tools that are specific to the laboratory experiment process * Introduces terminology used in many applications such as the interpretation of assay design and validation as well as fit for purpose procedures including real world examples * Includes a rigorous review of statistical quality control procedures in laboratory methodologies and influences on capabilities * Presents methodologies used in the areas such as method comparison procedures, limit and bias detection, outlier analysis and detecting sources of variation * Analysis of robustness and ruggedness including multivariate influences on response are introduced to account for controllable/uncontrollable laboratory conditions

This is the first book written on using Blender (an open-source visualization suite widely used in the entertainment and gaming industries) for scientific visualization. It is a practical and interesting introduction to Blender for understanding key parts.

Analytical techniques are powerful tools in a chemist's armoury and this book identifies some of the most important chemical techniques currently in use, along with their applications. Aimed at those with some familiarity with modern chemical techniques, as well as those completely new to them, the book covers much of the basic theory without emphasising the mathematics and physics involved. Where appropriate, descriptions of the instrumentation and sample preparation are included, as are problems with example solutions. More advanced ideas are presented in highlighted boxes, so the novice can happily skip these if desired. Modern Chemical Techniques is based on a series of 'hands-on' symposia that enabled individuals to update their chemical skills and learn about the newest methods, techniques, and instrumentation available. The resource material presented at the symposia is published here, developed and extended into an accessible, illustrated book, making the valuable information it contained available to a much wider audience.

This companion volume to "Fundamental Polymer Science" (Gedde and Hedenqvist, 2019) offers detailed insights from leading practitioners into experimental methods, simulation and modelling, mechanical and transport properties, processing, and sustainability issues. Separate chapters are devoted to thermal analysis, microscopy, spectroscopy, scattering methods, and chromatography. Special problems and pitfalls related to the study of polymers are addressed. Careful editing for consistency and cross-referencing among the chapters, high-quality graphics, worked-out examples, and numerous references to the specialist literature make "Applied Polymer Science" an essential reference for advanced students and practicing chemists, physicists, and engineers who want to solve problems with the use of polymeric materials.

In this book, the major paradigm-shifting discoveries made in the past century on key cellular nanomachines are described in great detail: their complex yet precise and elegant design and function, as well as the diseases linked to their dysfunction and the therapeutic approaches to overcome them. The major focus of this book is the "porosome" nanomachine, the universal secretory portal in cells. This is an ideal book for students, researchers, and professionals in the fields of nanoscience and nanotechnology.

Although the specimen of choice in the US drug testing industry is urine, and serum in clinical medicine, interest has recently grown in the use of other matrices as drug testing media. This book provides researchers and forensic toxicologists with a convenient general text summarizing the state of knowledge today. Chapters focus specifically on the application of these practices to drugs of abuse. The up-to-date information provided is complemented with thorough references.

Neutrons are extremely versatile probes for investigating structure and dynamics in condensed matter. Due to their large penetration depth, they are ideal for in-situ measurements of samples situated in sophisticated and advanced environments. The advent of new high-intensity neutron sources and instruments, as well as the development of new real-time techniques, allows the tracking of transformation processes in condensed matter on a microscopic scale. The present volume provides a review of the state of the art of this new and exciting field of kinetics with neutrons.

For some people with disabilities, their interest and skills are best applied to laboratory work. Science laboratories are environments where hazardous materials and processes are in use, and assessments are required to mitigate risk and ensure compliance with Occupational Safety and Health Administration (OSHA) and Environmental Protection Agency (EPA) regulations. Accommodating individuals in a laboratory requires balancing adherence to those regulations, as well as the Americans with Disabilities Act (ADA) technical access standards. Individualized assessment and accommodation are needed to ensure that a qualified individual with a disability can work or study effectively in the laboratory while ensuring a safe working environment for all. This book is intended to be a helpful guide for professionals to understand how to provide equal access to people with disabilities in a laboratory environment. It will review the breadth of protections that are provided by the ADA. This book also covers the roles and responsibilities of persons involved in laboratory oversight, including institutional policies and their limitations with respect to providing appropriate support for individualized assessments in the laboratory.

Algae are simple, primitive, heterogeneous, autotrophic, eukaryotic or prokaryotic organisms that lead a symbiotic, parasitic or free-living mode of life. Microalgae and macroalgae possess great potential in various fields of application. Microalgae are ubiquitous and extremely diverse microorganisms that can accumulate toxic contaminants and heavy metals from wastewater, making them a superior candidate to become a powerful nanofactory. Algae were discovered to reduce the presence of metal ions, and afterwards aid in the biosynthesis of nanoparticles. Since algae-mediated biogenic nanoparticles are eco-friendly, cost-effective, high-yielding, speedy and energy-efficient, a large number of studies have been published on them in the last few years. This book focuses on recent progress on the utilization of algae for the synthesis of nanoparticles, their characterization and the possible mechanisms involved. Bioprospecting Algae for Nanosized Materials describes the synthesis of algal nanomaterials and its application in various fields for sustainable development. This book outlines the procedures to prepare phyconanomaterials, techniques to utilize the nanomaterials, and applications in agriculture, environment and medicine.

This informationpacked book Great Personalities of India chronicles the lives of influential personalities whose achievements have helped shape and reshape the contours of India and its people. The compilation includes outstanding statesmen and women, entrepreneurs, scientists, social reformers, film artists, sportsmen and women, among others. Also included in the book are eminent people who have achieved distinction as writers, musicians, business persons, philanthropists, etc. Enjoy reading live and sacrifice of the 80 famous personalities who have influenced our lives, and are a source of constant inspiration and motivation for all of us. The text is organised categorywise for easy search and reference. Each biography includes life sketch, a trivia, a quote, successes & failures and awards associated with him/her to inspire the readers and satisfy their curious instincts. Open up the pages of the book to read the success stories, and discover for yourself how fast you can imbibe their traits to make it big in your life.

This book discusses fundamentally new biomedical imaging methods, such as holography, holographic and resonant interferometry, and speckle optics. It focuses on the development of holographic interference microscopy and its use in the study of phase objects such as nerve and muscle fibers subjected to the influence of laser radiation, magnetic fields, and hyperbaric conditions. The book shows how the myelin sheath and even the axon itself exhibit waveguide properties, enabling a fresh new look at the mechanisms of information transmission in the human body. The book presents theoretically and experimentally tested holographic and speckle-optical methods and devices used for investigating complex, diffusely scattering surfaces such as skin and muscle tissue. Additionally, it gives broad discussion of the authors' own original fundamental and applied research dedicated to helping physicians introduce new contact-less methods of diagnosis and treatment of diseases of the cardiovascular and neuromuscular systems into medical practice. The book is aimed at a broad spectrum of scientific specialists in the fields of speckle optics, holography, laser physics, morphology and cytochemistry, as well as medical professionals such as physiologists, neuropathologists, neurosurgeons, cardiologists and dentists.

Most cells will survive removal from the natural mic- environment of their in vivo tissue and placement into a sterile culture dish under optimal conditions. Not only do they survive, but they also multiply and express differen- ated properties in such a culture dish. A few cells do this in suspension, but most will need some kind of mechanical support substituting for their natural connections with other cells. The surface of a culture dish that might have to be coated is usually sufficient. The recent trend to standa- ization of conditions and the existence of commercial ent- prises with adequate funds and specializing in the needs of scientists were responsible for the tremendous proliferation of cell culture techniques in all fields of research in the last 20 years. No longer does a scientist have to concentrate all his/her efforts on that technology; the new trends make it feasible to employ cell culture techniques as only one of the many methods available in a small corner of a larger research laboratory. Some areas of research depend more heavily than others on cell culture techniques. Neuroscience is one of the areas that has developed hand in hand with the prol- eration of cell culture methodology. Molecular biological aspects, cell differentiation and development, neurophy- ological and neurochemical studies, as well as investigations into the nature of various diseases are now to a large extent dependent on the use of cell cultures.

Mammalian cells have evolved a complex multicomponent machinery that enables them to sense and respond to a wide variety of potentially toxic agents present in their environment. These stress responses are often associated with an increased cellular capacity to tolerate normally lethal levels of an insult. The realization that the mammalian stress response may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer, has led to an explosion of interest in this research area. Stress Response: Methods and Protocols brings together a diverse array of practical methodologies that may be employed to address various aspects of the response of mammalian cells to environmental stress. The p- tocols are carefully described by authors who have both devised and succe- fully employed them, and they represent a mixture not only of well-established techniques, but also new technologies at the leading edge of research. The areas covered include the detection and assay of stress-induced damage, the acti- tion of signal transduction pathways, stress-inducible gene expression, and stress protein function. Although no volume of this size can be comprehensive and the topics covered reflect a personal choice, it is hoped that it will prove of subst- tial interest and use to a wide range of research workers in the field.

The accurate measurement of temperature is a vital parameter in many fields of engineering and scientific practice. Responding to emerging trends, this classic reference has been fully revised to include coverage of the latest instrumentation and measurement methods.

Featuring:

• Brand new chapters on computerised temperature measuring systems and temperature measurement in medicine.

• Sections on noise thermometers, the development of photoelectric and two-wave pyrometers and the latest IEC (International Electrotechnical Commission) standards

• Coverage of fibre optic thermometers, imaging of temperature fields and measurement in hazardous areas.

• Examination of virtual instruments, fuzzy logic in temperature measurement and new methods for thermometer calibration.

A superb collection of time-tested, key techniques for investigating nervous system function, as well as the actions of drugs on the nervous system. The methods range from authoritative treatment of such well-established techniques as recording electrical activity in the brain, to practical coverage of state-of-the-art methods, including neural transplantation and antisense technology. The techniques described are particularly suitable for studying the mechanisms of action of psychotropic drugs and drugs of abuse, as well as the etiology and pharmacotherapy of neuropsychiatric diseases and neurodegenerative disorders. Several of the methods detailed are highly useful in screening potential drugs for the treatment of depression, anxiety disorders, schizophrenia, and Parkinson's and Alzheimer's disease.

This detailed book highlights recent advances in molecular imaging techniques and protocols, designed to be immediately applicable in global bio-laboratories. The chapters are categorized into seven major groups according to the reporter materials, such as imaging with passive optical readouts, activatable bioluminescent probes, functional substrates and luciferases, organic fluorescent probes, BRET probes, FRET probes, as well as with advanced instrumentation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Live Cell Imaging: Methods and Protocols aims to direct and inspire researchers into creating smarter, next-generation imaging techniques that are truly quantitative, highly sensitive, and readily comprehended, in the effort to engender deeper understanding of biological systems and break new ground in the research fields of life science.

* Inclusion of realistic 3D simulations that behave very much like the real thing. This isn't just setting a value and reading something off the screen. These incorporate the physicality of the experiments, which might mean positioning yourself so that a moving needle can be seen accurately by using your position to remove parallax. * Based on academic research into teaching online. * Coverage of all of the required practicals (AQA). * Inclusion of background information on each experiment. * Detailed accounts of how to perform the experiment for real or with the simulation. * The simulations have the Association for Science's Green Tick of approval.

This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization.